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991.
Yagüe C Bayarri S Conchello P Lázaro R Pérez-Arquillué C Herrera A Ariño A 《Journal of agricultural and food chemistry》2005,53(13):5105-5109
A multicolumn solid-phase extraction cleanup for the determination of organophosphorus (OP) and organochlorine (OC) pesticides plus PCB congeners in virgin olive oil is presented. The method involves dissolution of the olive oil in hexane, followed by a cleanup system using a diatomaceous earth column (Extrelut-QE) with reversed (C(18)) and normal (alumina) phase SPE columns. Determination of OPs was by GC-NPD, while the OCs and PCBs were analyzed using GC-ECD. Recovery assays for OPs varied from 81.7% to 105.3%, for OCs ranged between 74.3% and 99.4%, while for PCBs were from 60.1% to 119.2%. Quantitation limits ranged from 10 to 25 microg/kg olive oil for OPs, and from 1 to 6 microg/kg olive oil for OCs and PCBs. In the case of positive samples, the confirmation of pesticide identity was performed by ion-trap GC-MS/MS. The applicability of the method was assayed with 19 virgin olive oil samples collected from different olive mills of Aragón (Spain). Only one OP pesticide (acephate) was detected in one sample at a concentration of 10 microg/kg. Organochlorine pesticides were found in 5-47% of samples at very low levels ranging from 1.5 to 5.2 microg/kg. PCBs were found in 20-90% of samples, showing concentrations between 2.3 and 17.3 microg/kg. 相似文献
992.
The purpose of this study is to identify species, hybrids, and cultivars of edible Citrus species with high contents of diosmin as a functional compound and also to identify the developmental progress of the fruit in which it reaches maximum levels; these findings would be useful for extraction purposes and for the modulating effect of plant growth regulators on diosmin content to increase the level of this flavone. The results obtained reveal that the highest contents of diosmin are present in immature fruits of certain varieties of citron (Buda's finger) and lemon (Meyer), whereas the contents in the edible parts of the fruits are irrelevant from a pharmacological point of view. Similarly, it is shown that it is possible to increase the content of this flavone using hormonal treatments (6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid) during the early stages of fruit growth. 相似文献
993.
Silva BM Andrade PB Mendes GC Valentão P Seabra RM Ferreira MA 《Journal of agricultural and food chemistry》2000,48(7):2853-2857
The phenolic compounds present in 17 samples of Portuguese commercial and three homemade quince jams were analyzed by reversed-phase HPLC/DAD, to determine their authenticity. Two different extraction methods were needed for the complete definition of quince jams profiles, one of them including an Amberlite XAD-2 cleaning step. These analyses showed that all the samples presented a similar profile composed of at least eight identified phenolic compounds, several unidentified characteristic procyanidin polymers, and sodium benzoate as preservative of quince jams. Several samples also contained arbutin, suggesting that these quince jam samples were fraudulently adulterated with pear puree. 相似文献
994.
To enhance efficiency of breeding programmes for Sclerotinia sclerotiorum resistance in sunflower capitula, two separate resistance tests have been recommended. However, the time necessary to develop
genotypes with two types of resistance makes this impractical. A strategy to combine the two tests was consequently proposed
to reduce the number of seasons per selection cycle, but genetic studies were necessary to determine if it could be applied
in breeding programmes. This was the objective of this work. Data from two genetically different sunflower inbred lines and
their F1, F2 and backcross generations were analysed in two years to determine the genetics of resistance to S. sclerotiorum in capitula measured by application on the same plants of a mycelium test combined with an ascospore test. Effects of maternal
origin were detected when the reciprocal generations were evaluated for the mycelium test. This suggests the importance of
choice of the inbred line used as female in population formation by hybridisation. Progeny subjected to the mycelium test
did not show any change in relative reactions to two S. sclerotiorum isolates suggesting that ranking genotypes according to the results of combined S. sclerotiorum tests is repeatable across Sclerotinia isolates and experimental seasons. Moderate narrow sense heritability indicated that selection of the best F2 plants should
be effective. Genetic gain from selection is possible because a reduction of lesion areas produced by the mycelium test can
be expected. Additive gene effects contribute significantly to reduction in lesion area. Consequences of results in population
improvement for S. sclerotiorum resistance in capitula are discussed.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
995.
The primary gene pool (GP-1) of a crop is composed of gene reservoirs that cross easily with the domesticated, while the crosses
regularly produce fertile offspring. The GP-1 is further subdivided into cultivated and wild gene pools. The cultivated gene
pool encompasses commercial stocks of the crop, as well as landraces. The wild GP-1 of the crop comprises putative ancestors
and closely related species that show a fair degree of fertile relationships with the domesticate. Two South American wild
subspecies of cassava (M. flabellifolia and M. peruviana) were proposed as natural members of the wild GP-1 of the crop. Another Brazilian species(M. pruinosa) is morphologically so close to both wild subspecies that it may turn out as another member of the wild GP-1.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
996.
del Baño MJ Lorente J Castillo J Benavente-García O del Río JA Ortuño A Quirin KW Gerard D 《Journal of agricultural and food chemistry》2003,51(15):4247-4253
The distribution of six compounds with three different polyphenol skeletons have been studied in Rosmarinus officinalis: phenolic diterpenes (carnosic acid, carnosol, and 12-O-methylcarnosic acid), caffeoyl derivatives (rosmarinic acid), and flavones (isoscutellarein 7-O-glucoside and genkwanin), each showing a characteristic behavior and distribution during the vegetative cycle. Only in leaves were all six compounds present, and the highest accumulation rate was related with the young stages of development. Rosmarinic acid showed the highest concentrations of all the polyphenols in all organs. The distribution of this acid in leaves, flowers, and stems suggests that in the first stages of flower growth, levels were due to in situ biosynthesis, and in the last stages, the contribution of transport phenomena was increased. The antioxidant activity of six extracts with different polyphenolic composition was evaluated in aqueous and lipid systems. The results clearly suggest that rosemary extracts are excellent antioxidants in both aqueous and lipid systems. 相似文献
997.
A.-H. Fostier M. B. Falótico E. S. B. Ferraz A. C. Tomazelli M. S. M. B. Salomão L. A. Martinelli R. L. Victoria 《Water, air, and soil pollution》2005,166(1-4):381-402
A sampling and analysis program has been completed between 1995 and 1998 in order to determine mercury concentrations in water, sediments, soils and fish in the Piracicaba river basin, one of the most populated and industrialized regions in Brazil. In sediment, the average Hg concentrations varied between 33 ± 17 ng g?1 and 106 ± 78 ng g?1, in samples collected during the rainy and dry season, respectively. The same averages were also found for soil samples (35 ± 14 and 109 ± 61 ng g?1). In water, the total Hg concentration varied between < 1.1 and 24.0 ng L?1. In piscivore fish, up to 943 μ g Hg kg?1 was found. Our results show that all compartments undergo Hg contamination, in view of the levels found in pristine environments. Water contamination seems to be due mainly to diffuse processes of soil erosion and suspension of river bed sediment during the rainy season. Also untreated wastewaters generated by industrial activities and from domestic sewage appear to be potential non-point sources in the most industrialized and populated part of the basin. On the other hand, atmospheric transport of mercury originating from the Campinas Metropolitan Region could be another source of mercury contamination, especially in the basin headwaters. 相似文献
998.
999.
Marques RC Dórea JG McManus C Leão RS Brandão KG Marques RC Vieira IH Guimarães JR Malm O 《Public health nutrition》2011,14(4):661-669
1000.
Peres R. Badial Jos P. Oliveira Filho Paulo Henrique J. Cunha Didier Q. Cagnini Joo P. Araújo Jr Nena J. Winand Alexandre S. Borges 《Research in veterinary science》2011,90(3):443-450
Hepcidin is part of the innate immune system, and it plays a central role in the regulation of iron homeostasis. This peptide has been previously characterized in man, non-human primates, rat, mouse, dog, swine, cattle, horse, fishes, reptiles and birds but until now not in sheep. The aim of this study was to sequence, characterize and perform hepcidin expression analysis in different tissues collected from healthy sheep. The resulting open reading frame consisted of 249 bp predicted to encode an 82 aa peptide with a putative 23 aa signal peptide, a 34 aa pro-region and the 25 aa mature hepcidin. The deduced sequence of the sheep hepcidin precursor was most homologous to Bos taurus and Bubalus bubalis. Hepcidin was predominantly expressed in liver, although high expression was present in abomasum and lower level expression occurred in other tissues. These findings extend our comparative knowledge showing the relationship of sheep hepcidin to other mammalian hepcidins and will be helpful for additional studies on iron metabolism and inflammatory processes in sheep. 相似文献