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101.
Assessment of EST- and genomic microsatellite markers for variety discrimination and genetic diversity studies in wheat 总被引:22,自引:0,他引:22
Fiona Leigh Vince Lea John Law Petra Wolters Wayne Powell Paolo Donini 《Euphytica》2003,133(3):359-366
It is likely that in the near future sequence information from sequencing programmes and EST libraries will generate an abundance
of genic microsatellite markers. This study is focused on the assessment of their likely impact and performance vis-à-vis
their genomic counterparts. Microsatellites from two sources were used to assess the genetic diversity in 56 old and new varieties
of bread wheat on the UK Recommended List. A set of 12 microsatellite markers generated from genomic libraries and 20 expressed
sequence tag (EST)-derived microsatellites were used in the study, and the performance of both marker sets assessed. The EST-derived
or genic microsatellites delivered fingerprints of superior quality, amplifying clear products with few stutter bands. Diversity
levels as revealed bygenic microsatellites are similar to the few published results. The PIC values for the genic markers
were generally lower than those calculated for the genomic microsatellites, though advantages of both marker classes for variety
identification applications are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
102.
Linaria maroccana Hook. f. Ann., ‘Lace Violet’, Lupinus hartwegii ssp. cruikshankii Lindl. ‘Sunrise’ and Papaver nudicaule L. ‘Meadow Pastels’ seeds were directly sown into 105 cell plug trays and received either ambient light or supplemental high intensity discharge (HID) lighting. For each species, a 2 × 3 × 3 factorial was used with two light intensities during propagation, three transplant stages, and three night temperatures. Seedlings were transplanted at the appearance of 2–3, 5–6, or 8–9 true leaves. Transplanted Linaria and Papaver seedlings were placed at 5/11, 10/16, or 15/21 ± 1 °C night/day temperatures and Lupinus seedlings were placed at 15/24, 18/25, or 20/26 ± 2 °C night/day temperatures. For this study, the optimum production temperature for Linaria was 10/16 °C as the cut stems produced at 15/21 °C were unmarketable and production time was excessively long at 5/11 °C. At 10/16 °C, Linaria seedlings should be transplanted at the 2–3 leaf stage to maximize stem number, stem length and profitability. For Lupinus the optimum temperature was 15/24 °C due to long stems and high profitability per plant. Lupinus seedlings should be transplanted at the 2–3 leaf stage when grown at 15/24 °C to obtain the longest and thickest stems; however, $/m2 week was higher for plants transplanted at the 8–9 leaf stage due to less time in finishing production space. For Papaver, the 15/21 °C temperature was optimal as that temperature produced the longest stems in the shortest duration, resulting in the highest $/m2 week. At 15/21 °C Papaver plants should be transplanted at the 2–3 leaf stage. Supplemental HID lighting had no effect on any of the species. 相似文献
103.
The conversion of forests and farmlands to human settlements has negative impacts on many native species, but also provides
resources that some species are able to exploit. American Crows (Corvus brachyrhynchos), one such exploiter, create concern due to their impact as nest predators, disease hosts, and cultural harbingers of evil.
We used various measures of crow abundance and resource use to determine crows’ response to features of anthropogenic landscapes
in the Puget Sound region of the United States. We examined land cover and land use composition at three spatial scales: study
sites (up to 208 ha), crow home ranges within sites (18.1 ha), and local land cover (400 m2). At the study site and within-site scales crow abundance was strongly correlated with land cover providing anthropogenic
resources. In particular, crows were associated with the amount of ‘maintained forest’ cover, and were more likely to use
grass and shrub cover than forest or bare soil cover. Although crows did not show a generalized response to an edge variable,
they exhibited greater use of patchy habitat created by human settlements than of native forests. Radio-tagged territorial
adults used resources within their home ranges relatively evenly, suggesting resource selection had occurred at a larger spatial
scale. The land conversion pattern of new suburban and exurban settlements creates the mix of impervious surfaces and maintained
vegetation that crows use, and in our study area crow populations are expected to continue to increase.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
104.
There is debate among ecologists about whether total habitat area or patch arrangement contributes most to population and/or
community responses to fragmented or patchy landscapes. We tested the relative effects of patch area and isolation for predicting
bird occurrence in a naturally patchy landscape in the Bear River Mountains of Northern Utah, USA. We selected focal patches
(mountain meadows) ranging in elevation from 1,920 to 2,860 m and in size from 0.6 to 182 ha. Breeding birds were sampled
in each focal meadow during the summers of 2003 and 2004 using variable-distance point transects. Logistic regression and
likelihood-based model selection were used to determine the relationship between likelihood of occurrence of three bird species
(Brewer’s sparrow, vesper sparrow, and white-crowned sparrow) and area, isolation, and proximity metrics. We used model weights
and model-averaged confidence intervals to assess the importance of each predictor variable. Plots of area versus isolation
were used to evaluate complex relationships between the variables. We found that meadow area was the most important variable
for explaining occurrence for two species, and that isolation was the most important for the other. We also found that the
absolute distance was more appropriate for evaluating isolation responses than was the species-specific proximity metric.
Our findings add clarity to the debate between ecologists regarding the relative importance of area and isolation in species
responses to patchy landscapes. 相似文献
105.
Serean L Adams John F Smith Rodney D Roberts Achim R Janke Nick G King Harry Robin Tervit Stephen C Webb 《Aquaculture Research》2008,39(13):1434-1442
The robustness of Pacific oyster, Crassostrea gigas (Thunberg), sperm cryopreservation in the context of selective breeding based on family lines was investigated. Irrespective of egg density, high fertilization success was achieved with cryopreserved sperm when sperm:egg ratios of 1000:1 to 10 000:1 were used. Variation among replicate runs on the same oyster batches was minimal, indicating that cryopreservation and larval rearing procedures were repeatable. Twenty independent single male–female crosses were made to assess the utility of cryopreserved sperm in selective breeding. The fertility of unfrozen sperm was generally a poor predictor of cryopreserved sperm fertility. Based on D‐larval yields, 17 of the 20 crosses were likely to yield adequate spat for selective breeding (>105 D‐larvae from 1 million eggs), two were marginal (5 × 104 D‐larvae) and one was inadequate (4 × 103 D‐larvae). An alternative fertilization strategy to improve D‐yield from a given number of sperm was then tested. Fertilizing 10 million eggs at a sperm:egg ratio of 200:1 increased the total D‐yield when compared with fertilizing 1 million eggs at a sperm:egg ratio of 2000:1 for the same male–female pair. We conclude that, despite wide variation in fertility, cryopreserved sperm is useful for family production. 相似文献
106.
Little is known about the ecology of loggerhead turtles, Caretta caretta, in Canadian waters. However, Canada's eastern waters off the Scotian Shelf, Georges Bank and Grand Banks appear to be seasonal foraging habitats based on dates and locations where they have been captured as fishery bycatch. Estimates derived from data recorded by the international observer program (IOP) and used for this study suggest that thousands of mostly immature loggerheads have been captured in the Canadian pelagic longline fishery (PLF) in the western North Atlantic since 1999. These data suggest that the PLFs for tuna and swordfish are among the most important causes of incidental capture. Although U.S. and Canadian bycatch estimation methods differ and uncertainty associated with the estimates is high, it is clear from the IOP data that bycatch in the Canadian PLF is substantial and roughly within the same magnitude as that reported for the entire U.S. fleet in the North Atlantic for the same period (1999–2005). Analysis of bycatch observer data from the Canadian PLF and other empirical data suggests that fishing at temperatures below 20 °C using fish rather than squid bait and size-18 circle hooks could reduce loggerhead bycatch and mortality. However, research is needed to identify the best options and their commercial viability. Loggerheads that use Canadian Maritime waters are one of the least studied groups of loggerheads in the world, so systematic long-term studies on the ecology of loggerheads in Canada would help fill important information gaps and assist in developing effective recovery plans for this species. 相似文献
107.
Energy and resource consumption of land-based Atlantic salmon smolt hatcheries in the Pacific Northwest (USA) 总被引:1,自引:0,他引:1
John Colt Steve Summerfelt Tim Pfeiffer Sveinung Fivelstad Michael Rust 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):94-108
This paper evaluates the resource and energy requirements of six different types of land-based, hatchery production systems located in the U.S. Pacific Northwest: flow-through with a gravity water supply, flow-through with a pumped water supply, flow-through with pure oxygen, partial reuse system, partial reuse with heating, and a reuse system for the production of Atlantic salmon (Salmo salar) smolts. Key parameters used in the evaluation include direct energy, indirect energy, transportation energy, greenhouse gas emissions, and pollutant discharges.Power (electricity and natural gas) and feed energy accounted for the majority of the required energy for all the rearing option evaluated. The sum of the fixed capital and chemicals components accounted for less than 2–12% of the total energy budget for any rearing option. The energy efficiency (energy output/energy input) of the six options ranges from 0.97% for flow-through with pumped supply to 3.49% for the flow-through with gravity supply. The rearing options with the three highest energy efficiencies were flow-through with gravity supply (3.49%), partial reuse (2.75%), and reuse (2.64%).On a kg of smolt produced basis, the six rearing options showed a wide range in performance. The reuse system had the lowest water (2 m3 kg− 1) and land (0.13 m2 kg− 1) requirements and the third lowest total energy requirement (288 MJ kg− 1). The partial reuse system had the second lowest total power requirement (276 MJ kg− 1), a low land requirement (0.21 m2 kg− 1), and moderate water requirements (33 m3 kg− 1). The partial reuse with temperature control had the second highest total power requirement (657 MJ kg− 1) and land and water requirements similar to the partial reuse system without temperature control. The flow-through system with pumped water supply had the highest water (289 m3 kg− 1), land (2.19 m2 kg− 1), and energy requirements (786 MJ kg− 1) of any of the rearing options. By comparison, the flow-through system with gravity water supply had the lowest energy requirement (218 MJ kg− 1), a moderate land requirement (0.78 m2 kg− 1), and a high water requirement (214 m3 kg− 1). The ranking of the six rearing options based capital and operating costs are likely to be quite different from those based on energy, water, and greenhouse gas emissions. 相似文献
108.
正棉籽酸脱绒会危害育种家人身安全和潜在环境。需要1种无需用酸,能有效去除棉短绒且对棉种萌发无不利影响的脱绒方法。为了优化棉籽机械脱绒流程,美国农业部农业研究中心(USDA-ARS)棉花生产与加工部门(Lubbock,Texas)研发创制了一台棉籽机械脱绒样机(图1,图2)。测试设计了应用7种滚筒衬垫(1种是不用任何研磨材料,其余6种分别采用不同的研磨材料)、1个或2个辊刷"擦 相似文献
109.
Xi Liang John E. Erickson Wilfred Vermerris Diane L. Rowland Lynn E. Sollenberger Maria L. Silveira 《Journal of Crop Improvement》2017,31(1):39-55
Plant root architecture offers the potential for increasing soil water accessibility, particularly under water-limited conditions. The objectives of this study were to evaluate the root architecture in two genotypes of sorghum (Sorghum bicolor (L.) Moench) differing in root angles and to assess the influence of different deficit irrigation regimes on root architecture. The response of two sorghum genotypes, ‘Early Hegari-Sart’ (EH; steep root angle) and ‘Bk7’ (shallow root angle) to four irrigation treatments was investigated in two replicated outdoor studies using large pots. The results indicated that EH possessed steeper brace and crown root angles, fewer brace roots, greater root biomass, and root length density than Bk7 at deeper soil depths (i.e., 15–30 and 30–45 cm) compared with a shallower depth (i.e., 0–15 cm). Across the soil profile, EH had greater root length density and length of roots of small diameter (<1 mm) than Bk7. Accordingly, EH showed more rapid soil-water capture than Bk7. Different levels of irrigation input greatly affected root architecture. Severe deficit irrigation (25% of full crop transpiration throughout the season) increased the angle and number of crown roots, root biomass, and root length density compared with 75 and 100% of full crop transpiration treatments. Consequently, root system architecture can be effectively manipulated through both genotypic selection and irrigation management to ensure optimal performance under different levels of soil available water. 相似文献
110.
Tuyet Thi Anh Truong John Fry Phu Van Hoang Hoang Huy Ha 《Paddy and Water Environment》2017,15(4):931-941
The consumption of energy inputs in agricultural production has been increasing rapidly during the past decades. However, given the limitations and costs of non-renewable energy, increasing production while using the least energy possible has become a major concern of most nations. Prompted by this concern, we conducted a face-to-face survey of 90 farming households in Thai Nguyen Province, Vietnam, to find out how energy is being used in agriculture and, specifically, in their rice production. Through analysis of energy input–output balances, combined with economic efficiency analysis, a comparison was made of conventional and SRI methods of rice production. The study found that applying the SRI method can save around 23% of energy inputs, while increasing energy outputs by 11%. Economic benefits per hectare also rise by more than 8 million dong (USD 364) compared to those under the conventional cultivation system. The study also showed conflicts between the energy and economic balances for manual compared with machine ploughing operations. This study contributes to providing an overview of energy consumption in rice cultivation at the household level. Its findings can help stakeholders to assess current policies and make better decisions on the uses of energy in agricultural production. In addition, the comprehensive approach taken here to analysing energy use and efficiency could expand the analysis and comparison of energy uses at sectoral or activity level—still a new field in Vietnam and many other countries. 相似文献