Chickpea evolution has selected for contrasting phenological mechanisms among different habitats

Arguably the most important adaptive criterion in annual crops is appropriate phenology that minimizes exposure to climatic stresses and maximizes productivity in target environments. To date this has been achieved empirically by selecting among diverse genotypes in target locations. This approach is likely to become inadequate with pending climate change because selection is imposed on the outcome (flowering time) rather than the underlying mechanism (i.e. responses to daylength, ambient or vernalizing temperatures). In contrast to the cereals, in legumes the interaction between phenological mechanisms and environmental selection pressure is largely unknown. This paper addresses this shortcoming through photothermal modelling of chickpea germplasm from the world’s key production areas using a meta-analysis of multi-environment trials located from 49° N to 35° S. Germplasm origin had significant effects on temperature and daylength responsiveness, the former strongly correlated to vegetative phase temperatures at the collection or development site (r = 0.8). Accordingly, temperature responses increase from winter- to spring-sown Mediterranean and Australian material, and then to north, central & southern India. Germplasm origin also affects the relationship between photoperiod and temperature response. In Eastern Mediterranean material a strong negative relationship (r = −0.77) enables temperature insensitive genotypes to compensate through a strong photoperiod response. Clearly, chickpea evolution has selected for different phenological mechanisms across the habitat range. Given that under the anticipated global warming temperature sensitive cultivars will flower relatively earlier than those responding largely to photoperiod, it is important to exploit this diversity in developing better-adapted genotypes for future cropping environments.     

High gamma power is phase-locked to theta oscillations in human neocortex

We observed robust coupling between the high- and low-frequency bands of ongoing electrical activity in the human brain. In particular, the phase of the low-frequency theta (4 to 8 hertz) rhythm modulates power in the high gamma (80 to 150 hertz) band of the electrocorticogram, with stronger modulation occurring at higher theta amplitudes. Furthermore, different behavioral tasks evoke distinct patterns of theta/high gamma coupling across the cortex. The results indicate that transient coupling between low- and high-frequency brain rhythms coordinates activity in distributed cortical areas, providing a mechanism for effective communication during cognitive processing in humans.     

Intake restriction strategies and sources of energy and protein during the growing period affect nutrient disappearance, feedlot performance, and carcass characteristics of crossbred heifers.

Two trials were conducted to evaluate intake restriction, energy, and protein source on the performance and carcass merit of heifers limit-fed corn gluten feed. Trial 1 crossbred heifers (n = 140) were allotted to ad libitum wet corn gluten feed (WCGF)-hay, ad libitum WCGF-corn, WCGF-corn continuously limit-fed to achieve a gain of 1.1 kg/d or WCGF-corn fed in the following sequence: 70% of ad libitum for 20 d, ad libitum for 20 d, and 2 d common intake during the 84-d growing period. One-half of these restricted-refed heifers received a rumen-undegraded protein supplement, and the other half received a rumen degraded protein supplement. Heifers were fed a common finishing diet ad libitum subsequent to the growing period. Heifers offered ad libitum WCGF-hay and WCGF-corn were slaughtered at a common compositional fat end point. Heifers limit-fed WCGF-corn were slaughtered at the same time as heifers offered ad libitum WCGF-corn, regardless of subcutaneous fat cover. Limit-feeding WCGF-corn diets to growing heifers reduced ADG (P < or = 0.01) but did not compromise feed efficiency. Method of intake restriction, continuous or ad libitum-interrupted, and supplemental protein source did not affect combined growing-finishing performance. However, when fed for a common length of time, the average of the limit-fed heifers had lighter carcass weights (P < or = 0.01) and lower (P = 0.04) marbling scores. Heifers offered ad libitum WCGF-hay gained slower (P < or = 0.01) and less efficiently (P < or = 0.01) than heifers offered WCGF-corn. Trial 2 crossbred heifers (n = 222) were allotted to dry corn gluten feed (DCGF)-corn ad libitum or restricted to 80% of ad libitum for 42, 84, or 126 d; or ad libitum corn silage or DCGF-corn silage at 80% of ad libitum for 84 d. Feed efficiency (P = 0.07) and ADG (P = 0.08) tended to behave quadratically, being poorer for heifers limit-fed for 126 d during the growing period. Heifers limit-fed DCGF-corn gained more efficiently (P = 0.05) than heifers grown on ad libitum corn silage. Limit-feeding CGF-corn to growing beef heifers can be used to achieve moderate rates of gain without compromising feed efficiency. However, limit-feeding during the growing period may result in lighter weight carcasses with lower quality grades if not fed to the same fat end point as heifers grown with free access to concentrate.     

Understory Response to Repeated Thinning in Douglas-fir Forests of Western Oregon

This study investigated effects of a second-thinning entry on understory vegetation and tree regeneration development and understory vegetation composition. Study sites were located in the Coast Range and Cascade Range mountains of western Oregon and were dominated by Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) forests. Stands were initially thinned between 1975 and 1982 and parts of these same stands were thinned again approximately 20 yr later. Thinning stands a second time resulted in greater amounts of fern, graminoid, and open-site species, but the abundance of tree regeneration was not affected. Despite different site conditions, compositional patterns in the understory consistently shifted toward open-site early seral species following the second thinnings. These results suggest that the initial impacts of a second thinning are not simply predictable from studies in which only a single thinning was implemented. It is important to consider that vegetation trends were already influenced by the previous thinnings, and impacts of the second thinning are thus not as easily detectable. Within these limitations, repeated thinnings may be an effective management tool to maintain early seral species in older forests, while other aspects of understory vegetation and tree regeneration are less influenced in the short term.     

Stem breakage of trees and energy dissipation during rockfall impacts

The capacity of individual trees to dissipate the energy released by rockfalls has previously only been quantified based on data obtained from static tree-pulling tests or from dynamic impact tests on wood samples. We predicted that these data are not representative of the maximum amount of energy that can be dissipated by living trees during rockfall impacts. To test this prediction, we carried out rockfall experiments on a forested slope in the French Alps. To calculate the rock's energy before and after impact, rockfalls were filmed digitally. The recordings of nine impacts causing instantaneous breakage of Abies alba Mill. trees were analyzed in detail. An exponential relationship between stem diameter at breast height (DBH) and the maximum amount of energy a tree can dissipate was highly correlated for all of our experimental data. We applied this relationship to other tree species based on published fracture energies. The relationships obtained for Cedrus spp., Fagus sylvatica L. and Picea abies (L.) Karst. were significantly correlated with data from other dynamic impact tests in the field and with maximum bending moments obtained from tree-pulling experiments. Multiple linear regressions showed that impact height influences the energy that will be dissipated by an A. alba tree, particularly for trees with a DBH less than 15 cm. For trees with a DBH greater than 15 cm, the effect of impact height was minimal up to a height of 1 m. There was a strong relationship between the amount of energy dissipated by a tree and the horizontal distance between the impact center and the vertical central axis of the tree.     

Element concentrations in the xylem sap of Picea abies (L.) Karst. seedlings extracted by various methods under different environmental conditions

We used a Scholander pressure chamber to assess the effects of various extraction methods under different environmental conditions on element concentrations in xylem sap of 3-year-old Picea abies (L.) Karst. seedlings. Sap from excised shoots contained higher element concentrations when extracted at low than at high over-pressures. When comparing plants differing in water status, we found that a high extraction over-pressure introduced a systematic error into the data. For example, in well-watered non-transpiring plants relative to unwatered transpiring plants, potassium concentrations were 70% higher in sap extracted at 0.1 MPa over-pressure, but only 10% higher in sap extracted at 1.0 MPa over-pressure. Moreover, treatment effects depended on the time of day when the sap was extracted. Increased water flux in transpiring plants relative to non-transpiring plants resulted in reduced xylem sap element concentrations when samples were collected after 9 h of transpiration, but not after 4 to 6 h of transpiration. Drought had little effect on xylem sap element concentrations, indicating that rates of element release into xylem conduits, element depletion by growing tissues, and water flow maintained a balance that may prevent nutrient stress during short-term drought.