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排序方式: 共有385条查询结果,搜索用时 15 毫秒
71.
72.
Stephen AC Marklevitz Elizabete Almeida Joanna Flemming Jocelyne Hellou 《Journal of Soils and Sediments》2008,8(2):92-97
Background, Aims and Scope
Our study determined if and when an avoidance or attraction behavioural response would be displayed by Ilyanassa obsoleta when presented with a choice between harbour and reference sediments. Our goal was to develop a non-lethal approach that could be used in future assessments of the environmental quality of field sediments. 相似文献73.
Barrios-Rodiles M Brown KR Ozdamar B Bose R Liu Z Donovan RS Shinjo F Liu Y Dembowy J Taylor IW Luga V Przulj N Robinson M Suzuki H Hayashizaki Y Jurisica I Wrana JL 《Science (New York, N.Y.)》2005,307(5715):1621-1625
Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway to the p21-activated kinase (PAK) network, to the polarity complex, and to Occludin, a structural component of tight junctions. We show that Occludin regulates TGFbeta type I receptor localization for efficient TGFbeta-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions. 相似文献
74.
Joanna Burger 《Biological conservation》1981,21(3):231-241
I examined the direct and indirect effects of human activity on birds at a coastal bay refuge along the Atlantic Coast. Over the year, human activity varied at different sample sites on the refuge, but people were present on part of the refuge every day, although activity was concentrated on designated paths around a freshwater pond and at a fishing pier. On the refuge (exclusive of the ponds) people were present at the sample sites 17% of the time, birds were present 42% of the time when people were present, but birds were present 72% of the time when people were absent. Human activities, such as jogging or grass mowing, which involved rapud movement or close proximity to roosting birds, usually caused them to flush. Slow-walking bird watchers and clammers did not usually cause birds to flush. Gulls and terns were least affected as they usually relanded where they had been, ducks usually flushed and flew to the centre of the pond, and herons, egrets and shorebirds were most disturbed and flushed to distant marshes. These results suggest that if management objectives include providing roosting areas for migrating shorebirds then some areas must be protected from close and fast-moving human activities. 相似文献
75.
Katie A. McNaught Joanna S. Morris Mark McLaughlin 《Veterinary and comparative oncology》2020,18(3):292-302
Clusterin (CLU), also known as apolipoprotein J, is a widely expressed, heterodimeric, glycoprotein, important in tumourigenesis, apoptosis and immunoregulation. In humans, CLU expression has been associated with anaplastic large cell and Hodgkin's lymphoma. In this study, serum CLU levels in dogs with multicentric lymphoma (MLSA) were compared with healthy control dogs, using both western blot and enzyme‐linked immunosorbent assay (ELISA). Western blot confirmed the presence of CLU in dog sera at the predicted molecular weight and the relative levels detected correlated with the levels detected by ELISA. CLU level analysis by ELISA found treatment naïve dogs with MLSA had a significantly (P < .001) lower serum CLU level compared with healthy controls. However, there was no significant difference between MLSA dogs prior to treatment and in complete remission. The wide variation in serum CLU levels may limit its potential as a single candidate biomarker for MLSA, although any prognostic predictive value of serum CLU concentrations has yet to be assessed. 相似文献
76.
Justyna Połedniok Joanna Orzeł Justyna Gałeczka Rozalia Czoik 《Communications in Soil Science and Plant Analysis》2017,48(8):936-942
A simple and very sensitive spectrophotometric method determining microgram quantities of gallium (Ga) has been developed. The method is based on the complex of Ga(III) with Chrome Azurol S (CAS) in the presence of benzyldodecyldimethylammonium bromide (ST) and polyoxyethylenesorbitan monopalmitate (Tween 40). The complex forms in weakly acid solution (pH 3.8) and shows the absorption maximum at 627 nm. The molar absorptivity (ε) of this system is 1.87 × 105 L mol?1 cm?1, and it is clearly higher than ε of other spectrophotometric methods of gallium determination. The detection and quantification limits (LOD, LOQ) are 7.6 × 10–3 and 0.023 µg mL?1, respectively. Beer‘s law is obeyed in the range from 0.02 to 0.60 µg mL?1 of Ga. The precision and accuracy are good. This first spectrophotometric method of Ga(III) determination in the four-component system has been successfully applied for the analysis of mushrooms and grasses with low content of gallium. 相似文献
77.
Barbara Plytycz Malgorzata Klimek Joanna Homa Agnieszka Irena Mazur Jerzy Kruk A. John Morgan 《Pedobiologia》2011
Earthworm immune-competent cells (coelomocytes) can be adversely affected by soil metal contamination. The aim of the present paper was to perform comparative studies on the coelomocytes of four lumbricid species dermally exposed for 2 days to filter paper soaked with Ni, Cu, Zn, Cd, or Pb chlorides (1 mM). Coelomocytes were expelled by electro-stimulation, counted, and assayed ex vivo by flow cytometry and spectrofluorometry. The coelomocytes of Allolobophora chlorotica and Dendrobaena veneta contain both amoebocytes and riboflavin-storing eleocytes; consequently, they were subjected to assays based on flow cytometric quantification of autofluorescent eleocytes and spectrofluorometric measurement of riboflavin content in coelomocyte lysates. In Al. chlorotica, the number of coelomocytes, the percentage of eleocytes, and the amount of riboflavin were significantly lower in Cu-exposed worms although these cytometric parameters were less affected by Ni, Zn, Cd exposure, and entirely unaffected by Pb exposure. In D. veneta, such cellular effects were also only observed in Cu-exposed worms. The coelomic fluids of Aporrectodea caliginosa and Lumbricus rubellus are constitutionally impoverished of eleocytes; therefore, these species could not be subjected to assays measuring the riboflavin content of these granular cells. Rather, the amoebocytes of Ap. caliginosa and L. rubellus were subjected to flow cytometric measurements of in vitro neutral red uptake (NR). However, the NR uptake assay was technically demanding, requiring a strictly normalized incubation period over all samples to yield useful comparative data. In contrast, the riboflavin content in the coelomocyte lysates of eleocyte-rich species appears to be a robust and convenient immune-function biomarker of environmental stress. 相似文献
78.
Joanna Godek Kamila Milewska Angelika Tobolska ukasz Grabarczyk Wojciech Maksymowicz Isaac Bada Zbigniew Adamiak 《Anatomia, histologia, embryologia》2019,48(5):449-454
The aim of this study was to develop an anatomical model of the feline hip joint for low‐field magnetic resonance imaging (LF‐MRI) based on high‐field magnetic resonance imaging (HF‐MRI). The study was performed on six adult clinically healthy European shorthair cats, aged 1–3 years, with body weight of 2.8–4.4 kg. The animals were examined with the use of the Vet‐MRI Grande Esaote LF (0.25 T) scanner and high‐field Siemens Magnetom TRIO (3 T) MRI scanner. In the LF‐MRI, most satisfactory results in T1‐weighted images were obtained when TE was 26 ms in all three planes and when TR was 350–950 ms in the transverse plane, 950–1150 ms in the sagittal plane and 520–750 ms in the dorsal plane. In T2‐weighted images, TE was 90 ms in the transverse and dorsal plane and 120 ms in the sagittal plane. The results were presented as images acquired with LF‐MRI scanners in three planes. The slice thickness was 3 mm for each plane. In LF‐MRI, muscles in the hip joint region and round ligament were well visualized. Unlike in LF‐MRI, the cross section of the femoral nerve was identified in HF‐MRI scans. In examinations of the feline hip joint, the main limitations of LF‐MRI were a lack of reliable contrast between articular cartilage and synovial fluid as well as longer scan time. Despite the above, LF‐MRI images were characterized by good contrast between bones and the surrounding soft tissues. 相似文献
79.
Joanna Mucha Andrzej M. Jagodziński Bartosz Bułaj Piotr Łakomy Adrian M. Talaśka Jacek Oleksyn Marcin Zadworny 《Annals of Forest Science》2018,75(1):22
Key message
Quercus robur seedling mass was affected more by planting density than by taproot pruning. Root pruning enhanced stem biomass at the expense of roots in later growth stages. Alteration of biomass allocation due to nursery practices may result in greater susceptibility to injury and death of the seedlings under unfavorable environmental conditions.Context
Plants adjust their growth and modulate the resource allocation in response to applied treatments and environmental conditions.Aims
The aim was to examine how taproot pruning in seedlings grown at different densities affected long-term growth of Quercus robur.Methods
Seedlings, sown as acorns at two planting densities, with or without pruned roots were harvested in the second, fourth, and fifth years of growth. The effect of root pruning on biomass allocation was determined by measuring leaf, stem, and root mass fractions; carbohydrate concentrations in the roots; and C/N ratios. Specific leaf area and root length were also determined to assess morphological adaptations to growth conditions.Results
Total seedling mass was affected more by planting density than by taproot pruning. After 4 years of growth, root mass fractions were lower and stem mass fractions were greater in seedlings planted at a higher density. Five-year old root-pruned seedlings also had a lower root mass fraction and higher stem mass fractions than unpruned seedlings. Specific root length was not affected by root pruning or planting density.Conclusion
Decrease of relative root biomass with simultaneous increase of stem biomass may be a long-term consequence of taproot pruning of Q. robur, and the effects may manifest years after the seedling stage.80.