Sulfated ferulic acid is the main in vivo metabolite found after short-term ingestion of free ferulic acid in rats

The bioavailability of ferulic acid (FA; 3-methoxy-4-hydroxycinnamic acid) and its metabolites was investigated in rat plasma and urine after an oral short-term ingestion of 5.15 mg/kg of FA. Free FA, glucuronoconjugates, and sulfoconjugates were quickly detected in plasma with a peak of concentration found 30 min after ingestion. Sulfoconjugates were the main derivates ( approximately 50%). In urine, the cumulative excretion of total metabolites reached a plateau 1.5 h after ingestion, and approximately 40% were excreted by this way. Free FA recovered in urine represented only 4.9 +/-1.5% of the native FA consumed by rats. Glucuronoconjugates and sulfoconjugates represented 0.5 +/- 0.3 and 32.7 +/- 7.3%, respectively. These results suggested that a part of FA incorporated in the diet was quickly absorbed and largely metabolized in sulfoconjugates before excretion in urine.     

Conservation of Northern European plant diversity: the correspondence with soil pH

Effective biodiversity conservation requires an analysis of the existing reserve system. In temperate and boreal regions, plant diversity has a strong positive association with soil pH. Consequently, in order to protect plant diversity effectively, a relatively large proportion of protected areas should be on high pH soils. Since biodiversity data are never complete for all taxa, biodiversity indicators, e.g., threatened species, should be used. We studied soil pH distributions in protected areas in Northern Europe and tested whether soil pH requirement differs between threatened and non-threatened bryophyte and vascular plant species. As result, the proportion of high pH soils in protected areas was significantly greater than the proportion of these soils in general. This ensures that a large regional pool of plant species preferring high pH soils is relatively well protected. Threatened and non-threatened species in Northern Europe did not differ in their soil pH requirements, but threatened species required a narrower soil pH range than non-threatened species. Consequently, threatened species diversity can be used for indicating overall plant diversity.     

Isolation of 4-methylthio-3-butenyl glucosinolate from Raphanus sativus sprouts (kaiware daikon) and its redox properties

The most promising among glucosinolates (GLs) are those bearing in their aglycon an extra sulfur function, such as glucoraphasatin (4-methylthio-3-butenyl GL; GRH) and glucoraphenin (4-methylsulfinyl-3-butenyl GL; GRE). The GRE/GRH redox couple is typically met among secondary metabolites of Raphanus sativus L. and, whereas GRE prevails in seeds, GRH is the major GL in full-grown roots. During the 10 days of sprouting of R. sativus seeds, the GRE and GRH contents were determined according to the Eurpean Union official method (ISO 9167-1). In comparison to the seeds, the GRE content in sprouts decreased from about 90 to about 12 micromol g(-1) of dry weight (dw), whereas a 25-fold increase--from about 3 to 76 micromol g(-1) of dw--of the GRH content was measured. An efficient pure GRH gram-scale production process from R. sativus (kaiware daikon) sprouts resulted in significant yield improvement of up to 2.2% (dw basis). The reaction of GRH with both H2O2 and ABTS*+ radical cation was investigated. Whereas H2O2 oxidation of GRH readily resulted in complete transformation into GRE, ABTS*+ caused complete decay of the GL. Even though not directly related to its radical scavenging activity, the assessed reducing capacity of GRH suggests that R. sativus sprouts might possess potential for health benefits.     

Birds like it Corky: the influence of habitat features and management of ‘montados’ in breeding bird communities

In the southwest part of the Iberian Peninsula the dominant land-use are the Portuguese montados and Spanish dehesas, parkland forested areas of anthropogenic origin dominated by cork oak. They form a wooded matrix with open areas, scattered woodlands and undisturbed patches of Mediterranean forest and scrublands. The montados are characterized by a rich bird community. We have focus our study in a multidisciplinary approach, evaluating how management and landscape patterns influence the bird community in order to identify potential threats to its conservation. The study was conducted in the Site of Community Importance of Serra de Monfurado were 70% of the area is cork and holm oaks. We used data from 120 10-min point counts. Using variation partitioning, we determined the independent and joint effects of Forest, Management and Habitat variables. The variation captured in bird assemblage was 65.06%. Most of the explained variation was related to the Habitat and Management variables. The explanatory variables that were high-lighted as important predictors were variables that reflected tree and shrub density and cork removal. Modelling for forest species through generalized linear models (GLM) emphasize that the management plays an important role in the species distribution. The most important variables selected in models reflected cattle grazing and cork removal. Our results point out that the type of management is crucial to maintain the equilibrium in bird community associated to montados. Farmland and forest species will benefit from areas with different tree densities, small patches of Mediterranean scrubs in the understory and correct livestock numbers.     

Forest structure, composition, and tree diversity response to a gradient of regeneration harvests in the mid-Cumberland Plateau escarpment region, USA

Upland hardwood stands on mesic, escarpment-oriented sites on the Cumberland Plateau region of northeastern Alabama provide a myriad management opportunities. Stands are primarily managed for Quercus, but the high species diversity allows for management that targets multiple species. Stand composition is unique in that dominant species include shade tolerant species such as Acer saccharum, intermediate tolerant Quercus spp. and Carya spp., and intolerant Liriodendron tulipifera. Three replications of five levels of disturbance were created to assess species compositional changes; disturbances included three levels of harvest intensity, a mid-story herbicide treatment, and a control. After eight growing seasons, there were no discernable differences in species richness, diversity or evenness. Importance values based on relative basal area and relative density also changed little, except for clearcuts where L. tulipifera greatly increased. An initial gradient in basal area, canopy cover, and light created by harvesting or thinning dissipated following five growing seasons. Options exist for future stand management, including promoting two-aged or uneven-aged systems. Maintenance of Quercus will require additional tending.     

Impacts of tree canopy structure on wind flows and fire propagation simulated with FIRETEC

Introduction   

Forest fuel management in the context of fire prevention generally induces heterogeneous spatial patterns of vegetation. However, the impact of the canopy structure on both wind flows and fire behavior is not well understood.     

Collection of Lupinus angustifolius L. Germplasm and Characterisation of Morphological and Molecular Diversity

Lupinus angustifolius L. is a Mediterranean species, domesticated in the 20th century, representing an important grain legume crop in Australia and other countries. This work is focused on the collection of wild germplasm and on the characterisation of morphological and molecular diversity of germplasm accessions. It reports the collection of 81 wild L. angustifolius accessions from the South and Centre of Portugal, available at the ‘Instituto Superior de Agronomia Gene Bank’, with subsequent morphological and molecular characterisation of a selection of these and other accessions. A multivariate analysis of morphological traits on 88 L. angustifolius accessions (including 59 wild Portuguese accessions, 15 cultivars and 14 breeding lines) showed a cline of variation on wild germplasm, with plants from Southern Portugal characterised by earlier flowering, higher vegetative development and larger seeds. AFLP and ISSR molecular markers grouped modern cultivars as sub-clusters within the wider diversity of wild germplasm, revealing the narrow pool of genetic diversity on which domesticated accessions are based. The importance of preserving, characterising and using wild genetic resources for L. angustifolius crop improvement is outlined by the results obtained.     

Antipeptide antibodies recognizing plasmin sensitive sites in bovine beta-casein sequence

To investigate plasmin activity in cheese, we produced antibodies to bovine beta-casein with controlled specificity, suitable as markers of the integrity of the major bonds involved in its initial breakdown. Sixteen rabbits were immunized with synthetic substitutes for six plasmin-sensitive peptides. Antisera raised to the peptides (f20-39), (f40-56), (f94-113), (f184-202), and (f193-209) recognized beta-casein in ACP-ELISA, Western-blot, and biosensor assays. Casein in vitro hydrolysis by plasmin or chymosin reduced the detection of these determinants in ACP-ELISA, in agreement with the enzymatic sensitivity of bonds included within the binding sites, or in their neighborhood. Antiserum to (f20-39) in particular allowed the specific detection of plasmin cleavage at the bond generating gamma1-CN. Antisera to C-terminus preferentially detected the cleavage by chymosin. Immunoassays using these antibodies would allow in situ monitoring of significant proteolysis events without bias originated in the secondary degradation of the released peptides.     

Effect of heat treatment on the antioxidant activity of extracts from citrus peels

The effect of heat treatment on the antioxidant activity of extracts from Citrus unshiu peels was evaluated. Citrus peels (CP) (5 g) were placed in Pyrex Petri dishes (8.0 cm diameter) and heat-treated at 50, 100, or 150 degrees C for 10, 20, 30, 40, 50, and 60 min in an electric muffle furnace. After heat treatment, 70% ethanol extract (EE) and water extract (WE) (0.1 g/10 mL) of CP were prepared, and total phenol contents (TPC), radical scavenging activity (RSA), and reducing power of the extracts were determined. The antioxidant activities of CP extracts increased as heating temperature increased. For example, heat treatment of CP at 150 degrees C for 60 min increased the TPC, RSA, and reducing power of EE from 71.8 to 171.0 microM, from 29.64 to 64.25%, and from 0.45 to 0.82, respectively, compared to non-heat-treated control. In the case of WE from CP heat-treated at the same conditions (150 degrees C for 60 min), the TPC, RSA, and reducing power also increased from 84.4 to 204.9 microM, from 15.81 to 58.26%, and from 0.27 to 0.96, respectively. Several low molecular weight phenolic compounds such as 2,3-diacetyl-1-phenylnaphthalene, ferulic acid, p-hydroxybenzaldoxime, 5-hydroxyvaleric acid, 2,3-diacetyl-1-phenylnaphthalene, and vanillic acid were newly formed in the CP heated at 150 degrees C for 30 min. These results indicated that the antioxidant activity of CP extracts was significantly affected by heating temperature and duration of treatment on CP and that the heating process can be used as a tool for increasing the antioxidant activity of CP.