First Isolation of Rotavirus Associated with Neonatal Diarrhoea in Guanacos (Lama guanicoe) in the Argentinean Patagonia Region

Group A rotavirus (RV) and coronavirus (CV) are common viral pathogens associated with neonatal diarrhoea in numerous animal species. The purpose of this work was to investigate the presence of these viral agents in two farm populations of captured guanacos (Lama guanicoe) in the Argentinean Patagonia region, that developed severe diarrhoea outbreaks. Stool and serum samples were analysed for RV and bovine CV antigen and antibody by enzyme‐linked immunosorbent assay. Rotavirus was detected in faeces from two new‐born guanacos with acute diarrhoea, one in each farm. After electrophoretic analysis, each isolated strain, showed a distinctive long dsRNA electropherotype characteristic of group A rotaviruses (4:2:3:2). In addition, 95% (38 of 40) of the sampled animals were positive for RV antibodies, suggesting a high prevalence of RV infection in the populations tested. No evidence of CV circulation by antigen or antibody analysis was observed. To our knowledge, this is the first report of the detection and isolation of RV associated with neonatal diarrhoea in Lama guanicoe.     

Risk factors for stillbirths in two swine farms in the south of Brazil

We evaluated stillbirth risk factors in two commercial swine farms of the Rio Grande do Sul State (south of Brazil). The study was conducted during 1 month in Farm A and during 2 months in Farm B, both during 1999. Data for all farrowings that occurred during the study period were recorded (101 for Farm A and 373 for Farm B), without interference in the farm management. In Farm A, 39% of all litters born during the period of interest had stillborn piglets and the stillborn risk for piglets was 12%. In Farm B, 25% of all litters had stillborn piglets whereas the stillborn risk was 2%. Variables considered as potential risk factors for stillbirths were: parity (1, 2–3, 4+); breed (purebred or crossbred); sow body-condition (normal or fat); use of oxytocin during parturition (yes or no); obstetric intervention through vaginal palpation (yes or no); farrowing duration (<4 or ≥4 h); mummified fetuses (yes or no); total litter size (<12 or ≥12 piglets); and litter birth weight (<11 or ≥11 kg). All stillborn piglets had their classification validated by necropsy. In multivariable logistic-regressions, the cases were the litters having at least one stillborn piglet. In Farm A, litters having at least 12 pigs and in which oxytocin was used during the parturition had 20.8-times-higher odds of stillborn occurrence. In Farm B, litters from sows having parity ≥4 had 2.2-times-higher odds of stillborn occurrence than litters from parity 2 to 3 females, litters having ≥12 pigs had 2.0-times-higher odds of a stillborn piglet than smaller litters and farrowings in which vaginal palpation was performed had 8.0-times-higher odds. Farrowing room management to minimize stillborn risk should target higher-parity females, large litters and optimization of practices of obstetric interventions.     

Correlation between carcass condemnations in slaughterhouses and official notifications of cattle testing positive for tuberculosis

Tropical Animal Health and Production - The objective of this study was to analyze the correlation between condemnations in slaughterhouses situated in Minas Gerais State and notifications to the...     

Evaluating response to selection and nutritional needs in a three-way cross of rabbits

An experiment was carried out to evaluate the response obtained in a three‐way cross of rabbits when the lines involved had undergone within‐line selection for litter size or postweaning daily gain. The interaction between the level of nutrition and genetic level of the crossbreds was also studied. Using frozen embryos, old (H1) and current (H2) crossbred does and young were compared contemporarily. Does were fed a standard diet or a diet having high digestible energy and protein. The difference in the number of generations between lines used to produce both crosses were: 13 or 0 for the maternal lines, and 12 for the paternal line. The traits studied in the crossbred does were: daily feed intake during gestation, during the first 3 weeks of lactation and during the last week before weaning; doe weight at mating and at kindling; litter weight at birth and at weaning; total litter size, number born alive and litter size at weaning. The recorded traits for the young were: individual weight at weaning and at 63 days; individual daily gain after weaning; daily feed intake and feed conversion index. The last two traits were recorded as averages per cage. A total of 1161 litters from 190 does were involved. Significant differences in litter‐size traits, between H1 and H2 does, were observed and were higher than the expected from the within‐line response. The doe effect was not significant for other doe traits, adjusted to a constant litter size. The estimated difference in postweaning daily gain between H1 and H2 young was significant and in favour of H2, but the value was considerably lower than expected. The effect of type of feed and the interaction with the type of doe was not significant for any doe trait. The type of feed consumed by the doe was significant for postweaning daily gain, and the interaction with the type of crossbreds was significant for this trait and for individual weight at 63 days.     

Plasma endotoxin concentration in horses: a methods study

Background: Accurate determination of plasma endotoxin concentration is critical for ex vivo and in vitro cellular and molecular studies of endotoxemia in horses. However, reports are conflicting with respect to anticoagulant, handling, and sample preparation.

Objective:

Objective: The purpose of this study was to determine the effect of blood sample fraction and handling time on measurement of endotoxin concentration in horses.

Methods:

Methods: Whole blood, anticoagulated with 3.8% (0.12 M) sodium citrate (9:1), was collected from 5 healthy horses. Whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma (PPP) were spiked with endotoxin (2 EU/mL). Endotoxin-spiked WB samples were centrifuged immediately to generate PRP for measurement. Endotoxin concentration was subsequently measured by Limulus amebocyte assay at 0, 15, 30, 45, and 60 minutes. Assays were performed in triplicate and results were analyzed using Student's t -test, with significance set at P < .05.

Results:

Results: Mean endotoxin concentrations in 2 EU/mL-spiked WB were significantly different from those in PPP at all time points tested. Recovery of endotoxin in PRP generated from WB was significantly diminished after just 15 minutes.

Conclusion:

Conclusion: PRP generated from WB is significantly more reliable than PPP in determining endotoxin concentration ex vivo. Measurement of endotoxin in PRP generated from WB was significantly diminished after 15 min, identifying a time frame within which to process blood samples for endotoxin analysis.     

Involvement of the ERK1/2 MAPK pathway in insulin-induced S6K1 activation in avian cells

In mammals, insulin regulates S6K1, a key enzyme involved in the control of protein synthesis, via the well-documented phosphoinositide-3'kinase (PI3K) pathway. Conversely, S6K1 is activated by insulin in avian muscle despite the relative insulin insensitivity of the PI3K pathway in this tissue. Mitogen-activated protein kinase (MAPK) cascade is another insulin sensitive pathway. The aim of this study was to explore the potential involvement of the ERK1/2 MAPK pathway in the control of p70 S6 kinase (S6K1) in avian species. Firstly, we characterized ERK1/2 MAPK in various chicken tissues. ERK2 was the only isoform detected in avian species whatever the tissue studied. We also showed that ERK2 is activated in vivo by insulin in chicken muscle. The regulation and the role of ERK2 in insulin signaling were next investigated in chicken hepatoma cells (LMH) and primary myoblasts. Insulin stimulation led to ERK2 and S6K1 phosphorylation, and concomitantly increased kinase activity. U0126, an inhibitor of the ERK MAPK pathway, completely abolished insulin-induced S6K1 phosphorylation and activity in chicken myoblasts, whereas its effect was only partial in LMH cells. In conclusion, these results show that ERK1/2 MAPK is involved in the control of S6K1 by insulin in chicken cells, particularly myoblasts.     

Rural veterinary services in Western Australia: Part B. Rural practice

OBJECTIVE: To determine the current status of rural veterinary services in Western Australia. PROCEDURE: A questionnaire was sent to all eligible rural practitioners registered in 2006 and the replies were transferred to Microsoft Excel for analysis. RESULTS: Of the rural practitioners invited to participate in the survey replies were received from 67%. There were equal numbers of females and males. Their mean age was 44 years. Ninety per cent of respondents considered knowledge gained as an undergraduate was sufficient to equip them for practice, but only 60% considered their practical skills adequate. Thirteen per cent of those in rural practices in 2005 had left by 2006. Eighty-nine per cent of respondents were in mixed practice, the balance in specific species practice, such as equine, large animal and production animal consultancy. The majority of rural practitioners relied on servicing companion animals for their viability; 7% earned their income from servicing production animals only. Seventy per cent utilised merchandising and the sale of pet foods to supplement the income received from the traditional veterinary services and 34% found it necessary to earn an independent income. A quarter considered that rural practice did not have a future. CONCLUSION: The majority of rural practitioners in Western Australia depend on companion animals, not production animals, to remain viable, with very few operating production animal services. Poor remuneration is a major reason why veterinarians leave rural practice, and many find it necessary to supplement their income or develop an independent income.