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51.
Alcalase treatment for elimination of stickiness in pikeperch (Sander lucioperca L.) eggs under controlled conditions
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Elimination of egg stickiness is an important factor in artificial reproduction of pikeperch (Sander lucioperca L.). This study was conducted to evaluate the effectiveness of Alcalase enzyme to remove the adhesive layer of pikeperch eggs. The eggs were treated with Alcalase at 0.5, 1.0, 1.5, 2.0 and 5.0 mL L?1 or a milk/talc solution 2 min post insemination. Duration of exposure was 2 min in Alcalase and 60 min in milk/talc. The highest, albeit not significant, hatching rate (85.4%) was found with 1.5 mL L?1 of Alcalase, but hatching rates were similar in 0.5, 1.0 and 2.0 mL L?1. Hatching rates were significantly lower groups treated with 5.0 mL L?1 Alcalase enzyme (56.4%) compared to groups treated with milk powder and talc (61.3%). Nominally complete removal of adhesiveness was observed in 1.5 and 2 mL L?1. All Alcalase treatments led to significantly lower incubation duration compared with the traditional milk/talc treatment. The application of Alcalase successfully eliminated pikeperch egg stickiness in less time than with traditional milk/clay/talc methods. 相似文献
52.
Salman Naeem Vijay Baheti Jiri Militky Jakub Wiener Promoda Behera Azeem Ashraf 《Fibers and Polymers》2016,17(8):1245-1255
In this study, impregnation of iron chloride was carried out on needle punched web of waste acrylic fibers, which was subsequently carbonized under layer of charcoal by physical activation in high temperature furnace to produce iron impregnated activated carbon (FeAC). For comparison purpose, one more sample of activated carbon (AC) was prepared without impregnation of iron chloride. Both the webs were carbonized at 1200 °C with no holding time, and characterization of BET surface area, SEM morphology, EDX elemental analysis, XRD crystalline structure was performed. The FeAC web was used as adsorbent for the removal of methylene blue from aqueous solution. The dye removal percentage was investigated at different experimental parameters like different dye concentrations, adsorbent dosage, stirring speed and different pH. The obtained results were analyzed using linear and non-linear forms of Langmuir and Freundlich isotherms and adsorption kinetics (i.e. pseudo first order and pseudo second order model). 相似文献
53.
Martina Buckova Roman Licbinsky Vilma Jandova Jan Krejci Jana Pospichalova Jiri Huzlik 《Water, air, and soil pollution》2017,228(4):166
The article provides information about a new device, AlgaTox developed in the R&D project sponsored by the Technology Agency (n.TA02030179) and patented in Czech Republic (CZ 305687). Its functionality is based on the use of biosensor, and its main advantage is fast response rate. The toxicity detection is achieved through precise measurement of green algae oxygen production dynamics after their exposure to light of wavelength of 680 nm. Clark sensor with a resolution of 0.05% of the equilibrium oxygen concentrations and stability at a constant pressure and temperature of 0.1% of the equilibrium oxygen concentration at the 24-h measurement is used for the oxygen detection. Laboratory testing of the device has been made using silver nitrate, substance with known inhibitory effect on algae. Real samples of aqueous soil extracts and waste sample from old dried-up industrial tailing pond enriched with insecticide have been also tested. The values of oxygen production inhibition or stimulation determined with the new device in the evaluation of real samples were up to six times higher in comparison with the corresponding values of inhibition (stimulation) of growth rates determined by standard procedure. 相似文献
54.
Mapping tree root systems with ground-penetrating radar 总被引:3,自引:0,他引:3
A ground-penetrating radar (GPR) technique was used to study the three-dimensional distribution of root systems of large (DBH = 14 to 35 cm) oak trees (Quercus petraea (Mattusch.) Liebl.) in relatively dry, luvisoil on loamy deluvium and weathered granodiorite. We used a pulse EKKO 1000 GPR system, a profile grid of 0.25 x 0.25 meters, at 0.05 m intervals, and a signal frequency of 450 MHz, to assure resolution of about 3 cm in both directions (further increases in resolution up to 1 cm are possible with the system). Coarse root density was 6.5 m m(-2) of stand area and 3.3 m m(-3) of soil volume. Maximum rooting depth of the experimental oaks was 2 m, and the root ground plan was significantly larger (about 1.5 times) than the crown ground plan. Based on earlier studies of Quercus robur L. from floodplain forests, where the extent of the root systems was much smaller (root ground plan:crown ground plan ratio of 0.6), we conclude that the high root ground plan:crown ground plan ratio indicates less favorable conditions of water supply at the experimental site than in the floodplain forest. The ground-penetrating radar system is noninvasive and allows relatively rapid and repeated measurements of the distribution of coarse root systems of trees. 相似文献
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56.
Tomas Lošák Jaroslav Hlusek Jiri Martinec Johann Vollmann Jiri Peterka Radek Filipcik 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):313-321
Abstract Camelina (Camelina sativa (L.) Crtz., false flax) is described as a species requiring fewer inputs than other oilseed crops thus making it an interesting alternative in sustainable cropping systems. As information on the combined effects of nitrogen and sulphur on camelina yield and quality parameters is meagre, a pot fertilization experiment was carried out with nitrogen applied as NH4NO3 at three increasing rates equivalent to a range from 63 up to 127 kg ha?1. These treatments were combined with sulphur additions applied as (NH4)2SO4 to achieve a soil sulphate content of 25 or 45 mg kg?1, respectively (equivalent to 75 and 135 kg S ha?1). The medium and high nitrogen rates combined with the low sulphur fertilization level increased the number of branches per plant compared with the lowest nitrogen fertilization at the same sulphur rate. Camelina seed yield increased with increasing nitrogen doses at the same low sulphur level, whereas straw yield increased only at the highest rate of nitrogen. Thousand-seed mass increased at the highest nitrogen dose and with the low sulphur application rate. In addition, the increase in nitrogen fertilization reduced seed oil content from 39.8% to 37.1%. A highly negative correlation was observed between oil and protein content of seeds. Nitrogen fertilization increased total oil yield and total protein yield. Differences in crop parameters between the sulphur treatments were not significant statistically, although the higher sulphur treatment tended to increase seed yield as well as oil and protein content compared with the low sulphur treatment. Thus, the combined application of N and S as mineral fertilizers is only recommended when growing camelina on sulphur-deficient soils if the aim is to achieve both high oil and protein production. 相似文献
57.
Monika Moravkova Jiri Lamka Michal Slany Ivo Pavlik 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(1):99-102
IS901 RFLP analysis of 36 Mycobacterium avium subsp. avium (MAA) isolates from 15 pheasants (Phasianus colchicus) and two goshawks (Accipiter gentilis) from four pheasant farms was performed. Using this method, six different IS901 RFLP types (E, F, G, M, Q, and V) were identified. The distribution of IS901 RFLP profiles was tightly linked to individual flocks. Matching IS901 RFLP profiles observed in the present study indicate MAA transmission between pheasants and goshawks in the same locality. In two flocks, different pheasants within a flock as well as in various organs of five individual pheasants were found to have two distinct IS901 RFLP profiles. 相似文献
58.
59.
Laboratory Study of Stormwater Biofiltration in Low Temperatures: Total and Dissolved Metal Removals and Fates 总被引:1,自引:0,他引:1
Godecke-Tobias Blecken Jiri Marsalek Maria Viklander 《Water, air, and soil pollution》2011,219(1-4):303-317
Stormwater biofilters, which are recommended for application in both Water-Sensitive Urban Design and Low Impact Development, can remove up to 80% or 90% of total metals found in stormwater. However, their winter operation is a common concern. That was addressed in this study by investigating the metal removal effectiveness of replicate laboratory biofilter mesocosms at 2°C, 8°C and 20°C. As recommended for cold climate bioretention, coarse filter media were implemented and in the top 100 mm layer topsoil was added to increase the sorption capacity. Cd, Cu, Pb and Zn concentrations measured in the biofilter effluent were far below those in the influent and this significantly improved the treated stormwater quality. Contrary to a common notion that coarse media in the main filter body impair dissolved metal sorption, satisfactory removals of dissolved metals were found in this study with most metal burdens retained in the top layer of the filter in which the sorption capacity was enhanced by topsoil. Some metal uptake by the plants was also detected. Temperature did not affect Cd, Pb and Zn removals in general, but Cu removals increased with decreasing temperatures. This was explained by increased biological activities in the filters at warmer temperatures, which may have led to an increased release of Cu with dissolved organic matter originating from root turnover and decomposition of organic litter and debris. Furthermore, plant uptake and biofilm adsorption may also be influenced by temperature. However, even in the worst case (i.e. at 20°C), Cu was removed effectively from the stormwater. Further research needs were identified including the effects of road salts on stormwater biofiltration during the winter period. 相似文献
60.
Groth A Corpet A Cook AJ Roche D Bartek J Lukas J Almouzni G 《Science (New York, N.Y.)》2007,318(5858):1928-1931
DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone chaperone Asf1 and MCM2-7, the putative replicative helicase, are connected through a histone H3-H4 bridge. Depletion of Asf1 by RNA interference impedes DNA unwinding at replication sites, and similar defects arise from overproduction of new histone H3-H4 that compromises Asf1 function. These data link Asf1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand. 相似文献