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V. S. A. K. Sama K. Himabindu S. Bhaskar Naik R. M. Sundaram B. C. Viraktamath J. S. Bentur 《Euphytica》2012,187(3):393-400
Host plant resistance is the preferred management strategy for Asian rice gall midge (Orseolia oryzae), a serious pest in many rice-growing countries. Identification of simple sequence repeat (SSR) markers that are tightly linked to pest resistance genes can accelerate development of gene pyramids for durable/multiple resistance. Based on conventional and molecular allelism tests, we report herein that rice genotype Aganni possesses Gm8 gene, conferring hypersensitive independent (HR– type) resistance to gall midge biotypes GMB1, GMB2, GMB3, GMB4, and GMB4M. The gene Gm8 was mapped to chromosome 8 within a 400-kbp region, and the SSR markers RM22685 and RM22709 flank the gene closely. Using these closely linked flanking markers, nine other gall midge-resistant genotypes were identified as carrying the same gene Gm8. Through marker-assisted selection, Gm8 has been introgressed into an elite bacterial blight-resistant cultivar, Improved Samba-Mahsuri (IS). 相似文献
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S Naik N Bouladoux C Wilhelm MJ Molloy R Salcedo W Kastenmuller C Deming M Quinones L Koo S Conlan S Spencer JA Hall A Dzutsev H Kong DJ Campbell G Trinchieri JA Segre Y Belkaid 《Science (New York, N.Y.)》2012,337(6098):1115-1119
Intestinal commensal bacteria induce protective and regulatory responses that maintain host-microbial mutualism. However, the contribution of tissue-resident commensals to immunity and inflammation at other barrier sites has not been addressed. We found that in mice, the skin microbiota have an autonomous role in controlling the local inflammatory milieu and tuning resident T lymphocyte function. Protective immunity to a cutaneous pathogen was found to be critically dependent on the skin microbiota but not the gut microbiota. Furthermore, skin commensals tuned the function of local T cells in a manner dependent on signaling downstream of the interleukin-1 receptor. These findings underscore the importance of the microbiota as a distinctive feature of tissue compartmentalization, and provide insight into mechanisms of immune system regulation by resident commensal niches in health and disease. 相似文献
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Sabhavat Bhaskar Naik Dhanasekar Divya Nihar Sahu Raman Meenakshi Sundaram Preetinder Singh Sarao Kuldeep Singh Vattikuti Jhansi Lakshmi Jagadish Sanmallappa Bentur 《Euphytica》2018,214(3):53
The rice brown planthopper (BPH) Nilaparvata lugens (Stål) is one of the major pests of rice across Asia. Host-plant resistance is the most ecologically acceptable means to manage this pest. A rice breeding line RP2068-18-3-5 (RP2068) derived from the land race Velluthacheera is reported to be resistant to BPH populations across India. We identified a new R gene [Bph33(t)] in this line using advanced generation RILs derived from TN1 × RP2068 cross through phenotyping at two locations and linkage analysis with 99 polymorphic SSR markers. QTL analysis through IciMapping identified at least two major QTL on chromosome 1 influencing seedling damage score in seed box screening, honey dew excretion by adults and nymphal survival. Since no BPH R gene has been reported on chromosome 1, we designate this locus as a new gene Bph33(t) which accounted for over 20% of phenotypic variance. Scanning the region for candidate gene suggested two likely candidates a leucine rich repeat (LRR) gene and a heat shock protein (HSP) coding gene. Expression profiling of the two genes in the two contrasting parents and RILs showed induction of the HSP gene (LOC_Os01g42190.1) at 6 h after infestation while LRR gene did not show such induction. It is likely that the HSP represented Bph33(t). 相似文献
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Introgression lines (ILs) derived from interspecific crosses are a source of new genetic variability. A total of 55 ILs derived from two crosses Swarna × O. nivara IRGC81848 (population A) and Swarna × O. nivara IRGC81832 (population B) were characterized for yield and yield-related traits/QTLs. Segregation of 103 simple sequence repeat (SSR) markers associated with yield-related QTLs was studied. Population A showed an average of 12.6% homozygous O. nivara alleles and population B showed 10.6%. Interestingly, three SSR markers, RM223, RM128 and RM517, showed conspicuous pattern of segregation. The distribution of parental alleles at three loci RM223, RM128 and RM517 linked to yield-related traits was unique. These markers flanked to several yield-related QTLs. RM223, flanking to qyld8.3, was heterozygous in almost all the 55 ILs except in IL10-3S and IL131S. RM128 on chromosome 1 and RM517 on chromosome 3 were mutually exclusive in 46 out of 55 ILs. These 46 ILs showed either of the marker allele RM128 or RM517 from O. nivara but not both. IL166S had both RM128 and RM517 from O. nivara and the other ILs showed homozygous Swarna allele at RM517 except IL65S. Population structure assigned the 55 ILs to three sub-populations based on their genomic diversity. IL65S, IL166S, IL248S, IL7K and IL250K showed high yields in multi-location trials, and IL248S was released for cultivation as DRRDhan 40. 相似文献
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Shirugumbi Hanamanthagouda Manohar Poornananda Madhava Naik Nagella Praveen Hosakatte Niranjana Murthy 《林业研究》2009,20(3):268-270
The gymnemic acid contents in various organs of Gymnema sylvestre were investigated by High Performance Liquid Chromatography (HPLC) method. The results shows that the content of gymnemic
acid in various organs, obviously different, was 54.29, 31.66, 28.82, 27.67, 25.39, 20.56 and 1.31 mg·g−1 DW in shoot tips, flowers, nodes, leaves, internodes, roots and seeds, respectively. The highest gymnemic acid content (54.29
mg·g−1 DW) was found in shoot tip, 1.96 fold higher than that in leaves (27.67 mg·g−1 DW). Maximum quantity of gymnemic acid (35.39 mg·g−1 DW) was observed in the young leaves, which was 1.52 times higher than that in old leaves (23.07 mg·g−1 DW). The content of gymnemic acid in young, middle and old internodes was 26.47, 25.77 and 23.94 mg·g−1 DW, respectively, all lower than that in leaves (27.67 mg·g−1 DW), whereas the content of gymnemic acid in young, middle and old nodes was 27.96, 28.81 and 29.66 mg·g−1 DW, respectively, all higher than that in leaves. The study provides the scientific evidences for the rational development
and utilization of Gymnema sylvestre resources, since over exploitation of natural stands has caused depletion of these plants in nature.
Foundation project: This work was supported by University Grants Commission, New Delhi (No. 33-88/2007 (SR)) 相似文献
18.
Sean Pascoe Premachandra Wattage Debananda Naik 《Aquaculture Economics & Management (Blackwell Science)》2013,17(5-6):295-308
Abstract Over the last decade or so, a number of bioeconomic models have been developed with the expressed objective of identifying the optimal time to harvest farmed fish. The models explicitly incorporate growth rates, prices and costs, but have generally excluded risk except implicitly through the discount rate. Implicit in this is the assumption that risk is continuous, such that the probability of significant changes in key parameters (e.g. prices, mortality rates etc) is independent of time. In reality, these factors can vary over time. In this paper, the optimal harvesting strategies for two farmed species were analysed using a bioeconomic model. The model was based on previous models applied in the literature, with a new feed‐use component applied. The theoretical ‘optimal’ harvest strategy was compared with actual behaviour, and the factors that may affect divergences in the results examined. 相似文献
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The leaf nutritional status of mango orchards was assessed using diagnosis and recommendation integrated system (DRIS). The DRIS norms, which showed higher variance and lower coefficients of variation, are found to have greater diagnostic precision. As per DRIS indices, a relative deficiency for magnesium, zinc, and boron corresponding to relative sufficiency for calcium, nitrogen, phosphorus, sulfur, and potassium was detected in 9-year-old mango orchards. For the younger orchards (6–7 year old), the order of requirement of nutrients was found to be calcium > sulfur > potassium > boron > nitrogen > phosphorus > magnesium > zinc. Boron was found as the most yield-limiting elements in all age group of plants. When the DRIS indices were compared on the basis of soil pH, calcium and magnesium were most yield-limiting nutrients below pH 5.5, while nitrogen, zinc, and boron were found to be most limiting above pH 5.5. 相似文献
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