Effects of Smo gene silencing on cell activity and apoptosis of human cervical carcinoma HeLa cells

AIM: To investigate the effect of Hedgehog (Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS: Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the HeLa cells was determined by RT-PCR and Western blot, respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS: Compared with control group, the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h (P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G₀/G₁ phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION: Smo gene silencing effectively inhibits the cell growth and induces the apoptosis of human cervical carcinoma cells.     

水生动物肌醇营养研究进展

肌醇是大多数水生动物保持健康、促进生长发育必需的营养素,它在细胞膜磷脂合成、神经递质传递和肝脏脂质转运等方面发挥重要作用。饲料中肌醇缺乏导致水生动物生长不良、体表充血、鳍条糜烂、体色发黑、脂肪肝等症状。本文综述了肌醇的理化性质,肌醇在水生动物营养中营养生理作用、需要量及影响因素等,同时还介绍了肌醇的来源和测定方法,并展望了肌醇在水生动物营养中进一步的研究和应用方向。     

藏青杨虫瘿的空间分布格局

为明确蚜虫在西藏工布自然保护区藏青杨(Populus szechuanica var.tibetica)叶片上的危害特征及其产生虫瘿的分布特点,基于野外调查数据,应用扩散系数(C)、Morisita指数(I)、Cassie指标(Ca)、平均拥挤度(M*)、Lloyd聚块性指标、负二项参数(K)、Iwao回归分析法、Taylor幂指数法对该区藏青杨叶片上虫瘿空间分布格局进行研究。结果表明,藏青杨叶片左上虫瘿出现率为25.36%、左下为24.30%、右上为24.98%、右下为25.36%,叶片左边为49.66%、右边为50.34%;藏青杨不同方向叶片上虫瘿出现的比率均在25%左右。藏青杨叶片上虫瘿主要以3~7个聚集的形式存在。虫瘿出现频次(y)与虫瘿数量级别(x)的拟合曲线为y=-2.150 3x~2+23.159x-7.309 1(R~2=0.818 4)。6级虫瘿数量最多,占总虫瘿数的22.15%。由虫瘿空间分布参数可知,藏青杨叶片上虫瘿空间分布呈聚集型,但虫瘿聚集强度不大。总体上虫瘿个体群大小随虫瘿密度的增加而增大,个体群指数L值平均大小为7.20。经拟合,虫瘿空间分布格局Iwao M*-M回归模型为M*=1.059 9m+0.523 4(R~2=0.993 9),Taylor模型为log S2=1.043 4logm+0.043 7(R~2=0.996 4),两模型均判定为聚集分布。综上,虫瘿在藏青杨不同方位的出现率相近,但在叶片上均呈聚集分布,虫瘿的聚集除与环境因素有关外,也与其生物学特性相关。     

饲粮添加硬脂酰乳酸钠对断奶仔猪生长性能、血清生化指标及养分表观消化率的影响

本试验旨在研究硬脂酰乳酸钠(SSL)对断奶仔猪生长性能、血清生化指标及养分表观消化率的影响。试验选择25日龄的断奶仔猪336头,按体重随机分为6组,每组7个重复,每个重复8头猪。各组仔猪饲粮中SSL添加水平分别为0(对照)、250、500、750、1 000和2 000 mg/kg,试验分2个阶段,每阶段21 d。试验测定仔猪生长性能,谷丙转氨酶、谷草转氨酶、总胆固醇等血清生化指标及能量、干物质、氮和粗脂肪表观消化率。结果表明:与对照组相比,1)饲粮中添加500、750和1 000 mg/kg SSL能显著降低仔猪第2阶段料重比(P0.05),添加1000 mg/kg SSL显著降低全期料重比(P0.05);2)饲粮中添加SSL有降低试验第42天仔猪血清谷草转氨酶、谷丙转氨酶活性,增加血清高密度蛋白胆固醇含量及高密度蛋白胆固醇/低密度蛋白胆固醇值的趋势,且当添加水平为2 000 mg/kg时差异达到显著水平(P0.05);3)饲粮中添加SSL能显著提高粗脂肪表观消化率(P0.05),并且有提高氮及能量表观消化率的趋势,当添加水平为2 000 mg/kg时差异达到显著水平(P0.05)。结果提示,饲粮添加SSL能降低仔猪料重比,提高饲料养分尤其是粗脂肪的表观消化率。     

Mapping QTL of Fiber Yield and Quality Traits in F2 Populations of Chromosome Segment Substitution Lines from Gossypium hirsutum × Gossypium barbadense

In this study, F₂ populations from two chromosome segment substitution lines MBI7455 and MBI7358, were quantified using SSR to evaluate the parents' genotype and detect QTL related to fiber quality plus yield traits of cotton. Results show that the recurrent parent (CCRI45) hosted 96.70% and 95.60% of chromosome segment substitution in MBI7455 with 12 chromosome segments and in MBI7358 with 16 chromosome substitution segments of Gossypium barbadense, respectively. In the F₂ population, the average rate of chromosome substitution of the recurrent parent (CCRI45) was 96.44%, and the average segments of chromosome substitution of Gossypium barbadense was 13.42, with an average segments of homozygous donor chromosome value of 3.90. Analysis showed 19 fiber quality-related QTL with a phenotypic variance of between 2.52%-13.11% and seven yield traits-related QTL with a range of 2.93%-11.40% phenotypic variance, resulting in a total of 26 QTL. The CSSLs could be used to detect QTL for fiber yield and quality traits, which offer an important foundation for the cotton molecular-assisted breeding.     

浅谈油气管道完整性管理模式

本文基于国内外油气管道完整性管理(PIM)成果的基础，从数据收集和研究、高后果区分析、完整性评估、管道检测与执行、管道维护修复、效能分析等方面进行具体PIM描述，从体系文件、标准规范、系统平台、支持技术、实施应用五个层面构建PIM管理体系框架。     

基于RS与GIS的山东某煤矿区历史遗留损毁土地调查

我国地域辽阔，但可利用土地资源少，同时由于生产建设和自然灾害等，造成许多优质土地资源损毁。调查清楚土地损毁情况，采取针对性的土地复垦措施，在目前经济发展中显得尤为迫切。以山东某矿区为例，确定了历史遗留损毁土地的划定依据，利用RS与GIS等技术手段对矿区进行地表沉陷预计，用遥感影像提取土地利用变化情况，得到了历史遗留损毁土地的位置、面积和损毁类型等信息，为后期土地复垦与规划提供参考。      

Design and Experiment of Slave Computer Control System for Applying Variable-rate Liquid Fertilizer

In order to increase the applying rate of liquid fertilizer and reduce environmental pollution, a slave computer control system for applying variable-rate liquid fertilizer was designed. The system used SMC as core processor and electrically controlled pressure regulator as execution component. The characteristic equation of the system was obtained by using classical control theory. Results indicated that the characteristic equation met the requirements of routh-criterion, which indicated the working process of the system was stable. Performance of the slave computer was verified via bench tests. Results demonstrated that there was no significant influence on the response from interclass error. The fertilization error was less than 0.9, and the fertilization accuracy was larger than 97%. The liquid fertilizer emitted by the fertilizing devices had no significant difference in uniformity, which met the demands of the slave computer control system for applying variable-rate liquid fertilizer.     

间接免疫荧光染色检测鸭源副黏病毒及在鸭体内的抗原定位

以抗新城疫病毒F蛋白的单克隆抗体为一抗,建立了间接免疫荧光染色法(IFA)检测石蜡切片中鸭副黏病毒(DPMV)的方法。以建立的IFA对DPMV人工感染鸭的各组织器官进行检测,结果显示:各组织器官均能检测到DPMV,但不同时间取样,阳性信号分布的器官不同。脾脏、胸腺、法氏囊、肠道、肝脏、肺脏DPMV的阳性检出率较高,表明这些器官为DPMV的主要靶器官。在所检测的阳性组织中,病毒抗原分布在细胞浆中。IFA检测石蜡切片中的DPMV具有直观、特异性强的优点,是对DPMV进行检测和抗原定位的良好方法。