Cryptosporidiosis in buffalo calves (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>): Prevalence and potential risk factors

The objective of the present study was to describe the prevalence and risk factors associated with cryptosporidiosis in buffalo calves in Middle Egypt. During one year, 458 fecal samples were collected from buffalo calves less than 3 month age in 55 small scale herds and examined for the presence of Cryptosporidium oocysts. Data describing age, gender, season, and herd management practices were gathered to assess potential risk factors. Fecal examination showed that 14.19% of the examined calves were positive for Cryptosporidium spp. Calves at 1-15 days were at the highest risk (P < 0.001), and a significant relationship between season and infection (P < 0.05) was recorded. A significant association between infection and hygiene (P < 0.001), type of floor (P < 0.01) and source of water (P < 0.01) was also recorded. Statistical analysis concerning the clinical signs and fecal characteristics revealed a significant association with fecal consistency (P < 0.001), presence of blood (P < 0.01) and mucous (P < 0.01). Moreover, a significant association was found between infection and the desire for suckling (P < 0.05) and tenesmus (P < 0.05). The results of the present study demonstrated the strong relation between infections by Cryptosporidium spp. and diarrhea in buffalo calves.     

8种豆科灌木栽培种丛枝菌根真菌种类及分布

采集人工栽培决明(Cassia tora)、假木豆(Dendrolobium triangulare)、紫穗槐(Amorpha fruticosa)、白刺花(Sophora davidii)、猪屎豆(Crotalaria pallida)、多花木蓝(Indigofera amblyantha)、木豆(Cajanus cajan)和银合欢(Leucaena leucocephala)8种豆科灌木植物根际5-10、10-20和20-30 cm 3个土层的土样,研究其根际丛枝菌根(AM)真菌的侵染率、孢子密度、种类及多样性。结果表明,8种豆科灌木植物AM真菌侵染率普遍较高,最高的为猪屎豆,80.8%;土壤速效磷含量与侵染率呈显著负相关(r=-0.733,P=0.039);根际土壤中的AM真菌孢子主要分布于5-20 cm土层,5-10 cm土层猪屎豆孢子密度最大,为115.33个·10 g-1土壤;宿主植物种类与土壤层位对孢子密度交互效应影响显著(F=100.497,P0.001);AM真菌优势种枫香球囊霉(Glomus liquidambaris)、脆无梗囊霉(Acaulospora delicate)在8种豆科灌木植物的土壤中均有分布。本研究表明,喀斯特地区适生豆科灌木植物具有丰富的AM真菌资源,今后可以在喀斯特地区开展豆科灌木植物接种优势AM真菌和根瘤菌等相关研究,这将对农牧业生产和退化生态系统修复效应的影响具有重要意义。     

湖南省长沙市动物卫生监督执法工作调研

通过对湖南省长沙市动物卫生监督所提升行政执法能力探索的综述,分析了现有制度存在发展理念滞后、体制机制未理顺、监督力量薄弱、执法手段落后、法律法规不够完善等问题,建议从统筹规划、监管体系、工作保障、宣传引导等方面入手,全方位提升动物卫生监督执法能力。     

不同浓度乙醇对蜂胶中的铅提取效果的研究

采用不同浓度的乙醇90％、85％、80％、75％、70％、65％、50％，对蜂胶的有效成分进行提取，测定其提取液和残渣中的铅含量，并计算其提取率。结果表明：随乙醇浓度的上升，提取率及提取液中的铅含量增高。其中乙醇浓度为75％、70％和65％的蜂胶提取液中的铅含量均小于1ppm，符合我国食品卫生法关于食品中的铅含量不得超过1ppm的规定。     

胆固醇转运蛋白NPC1L1的研究进展

NPC1L1是近年来人们研究高脂血症的重点内容,该蛋白已被证实在胆固醇的肠道吸收和胆汁分泌中发挥了关键作用。NPC1L1调节体内胆固醇的生物合成,是维持生物体胆固醇动态平衡的重要因素,同时也是新型降脂药物依泽替米贝的作用靶点。     

Circulating oxidative stress status in dromedary camels infested with sarcoptic mange

Oxidative stress is an imbalance between radical-generating and radical-scavenging activity, resulting in oxidation products and tissue damage. This study was aimed to evaluate the status of oxidative stress indices in blood of camels naturally infested with S. scabiei. Forty-seven male camels (Camelus dromedaries) were divided according to the extent of the infested area with Sarcoptes scabiei into four groups, mild (MID, n = 12), moderate (MOD, n = 10), severely infested (SEV, n = 10) and healthy control group (n = 15). Blood was used for determination of red cell count (RBC), hemoglobin (Hb), packed cell volume (PCV), serum nitric oxide (NO^•, a free radical), ascorbate and albumin concentrations, and erythrocytic values of malondialdehyde (MDA, a marker of lipid peroxidation), protein carbonyls (PC, an indicator of protein oxidation), glutathione (GSH) superoxide dismutase (SOD) and catalase (CAT). Decreased levels (P < 0.05) of RBC, Hb, PCV, albumin and ascorbate were noticed in MOD and SEV compared to controls with the lowest values (P < 0.05) in SEV except for ascorbate, where MOD did not differ from SEV. Compared to controls, NO^• gradually increased (P < 0.05) in MID followed by MOD and SEV, whereas MDA and PC were higher (P < 0.05) in MOD and SEV. PC was higher (P < 0.05) in MOD than SEV. In addition, the antioxidants GSH, SOD and CAT were higher (P < 0.05) in MID and lower (P < 0.05) in MOD and SEV compared to controls. GSH was lower (P < 0.05) in SEV compared to MOD. Besides, Hb was negatively correlated with NO^• (r = −0.68, P < 0.001), MDA (r = −0.53, P < 0.001) and PC (r = −0.73, P < 0.001). In conclusion, dromedary sarcoptosis is accompanied by a state of oxidative stress process, which increased by increasing the area of infestation, and may contribute to the pathogenesis of the disease.     

利用氨基酸指标评价鱼粉质量及掺假鉴别

鱼粉是一种重要的优质动物蛋白质原料,富含各种必需氨基酸,通过对鱼粉样品氨基酸数据的分析,总结出合格、低质、掺假鱼粉的氨基酸含量变化规律,提供了运用氨基酸指标评价鱼粉质量及掺假鉴别的方法。     

苜蓿雄性不育系杂交制种利用的研究

通过近20年的研究,已选育出遗传性稳定的苜蓿雄性不育系Ms-4,该不育系属于完全不育的类型,适合于制种利用;已寻找确定出强优势的杂交组合--Ms-4×新疆大叶苜蓿,该组合小面积试验比对照增产58.4%,可供杂交制种之用;已开始小面积的杂交制种试验,并已获得杂交种种子,现在正在进行品比、区试和生产试验,预计到2000年我国自己制种出来的第一个杂交苜蓿即可投入生产上推广,该项成果可望成为21世纪我国苜蓿大幅度增产的先进措施之一.     

Frequent use of colistin-based drug treatment to eliminate extended-spectrum beta-lactamase-producing <Emphasis Type="Italic">Escherichia coli</Emphasis> in backyard chicken farms in Thai Binh Province,Vietnam

Reports of livestock infections with extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E) are increasing. Based on interviews conducted over a 6-month period, we found that veterinarians in the Vietnamese province of Thai Binh prefer to prescribe colistin-based drugs (CBD) in chicken farms. We aimed to clarify whether CBD use selects for strains of colistin-resistant ESBL-E. With the cooperation of seven local households, we detected ESBL-E in chickens’ feces after treating chickens with CBD. Phylogenetic groupings and the presence of CTX-M/AmpC genes were determined, and the multi-antibiotic susceptibility of isolates was analyzed. Our results showed that ESBL-E presented in seven chickens’ feces from two households. Seventy-two percent of ESBL-E isolates harbored CTX-M9 and the phylogenetic group A; the colistin minimum inhibitory concentration (MIC) of all isolated ESBL-E ranged from 0.064 to 1 μg mL^?1. Moreover, ESBL-E isolates were used to experimentally select for colistin resistance, and the effect of commercial CBD on ESBL-E was investigated. The results showed that an ESBL-E strain with a colistin MIC of 4 μg mL^?1 was able to grow in media with CBD. Although CBD treatment was effective, in vitro experiments demonstrated that ESBL-E can easily acquire colistin resistance. Therefore, restrictions on colistin use are necessary to prevent the emergence of colistin-resistant bacteria.     

牛奶来源质量监督调查报告

为保证牛奶来源的质量和食品安全,对42个牧场进行随机检查,发现挤奶环境差、现场管理不当、人为搀假问题严重.其中酒精实验不合格和干物质低的牛奶数量最多,酒精阳性奶共48.27 t,干物质低奶54.36 t,分别占总问题奶的37%和44%.希望引起广大奶牛养殖户和乳品企业的重视.