Molecular cloning,purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.     

The Unified Representation of Interface Conditionsof Electromagnetic Quantities Using Exterior Differential Forms

A continuity condition of p-forms across a p-dimensional submanifold isderived.The,various interface conditions of electromagnetic quantities are obtained through theapplication of the continuity condition to Minkowski manifold of space-time.     

有机无机肥料对小麦的肥效及其交互作用

在京郊大同肥力水平土壤上,有机肥和化肥配合施用小麦增产量最高,其增量在中、高肥力试验点上大于低肥力试验点.有机肥和化肥配合施用时的增产作用是它们各自增产效应之和,它们之间的交互效应不显著.在高肥力土壤上甚至有负的交互效应,即配台施用时的增产量小于分施时各自增产量之和.根据肥料试验,本文提出了京郊不同肥力水平土壤上小麦有机无机肥料配合施用量,并发现在低肥力土壤上随着有机肥的施用,化肥氮的用量增加,化肥磷用量可减少;在高肥力土壤上随有机肥的施用,化肥氮用量减少,化肥磷用量增加;在中等肥力土壤上配合有机肥的施用,氮磷化肥用量均个应减少,应略为增加.     

厚膜集成电路STRS412的工作原理与维修

本文论述在电视机中普遍应用的厚膜集成电路STRS412的内部结构、外围电路的工作过程及典型故障检修。     

鸡沙门氏菌病的中西药结合治疗试验

贵阳市乌当区某个体养鸡场鸡群发病,诊断为鸡伤寒沙门氏菌不同血清型的菌株混合感染,药敏试验表明分离菌株对部分β-内酰胺类抗生素、四环素、氟喹诺酮类、红霉素、磺胺类具有不同程度的耐药性.笔者用中药黄芩、土大黄结合阿莫西林进行治疗,取得较好效果,现报道如下.     

北疆超晚播小麦品种生育特性及产量比较

为探明北疆超晚播条件下,冬、春性小麦品种间生育特性及产量的差异,采用田间试验（10月31日播种,冬前不灌水）,比较了6个冬性和5个春性小麦品种的生育特性及产量。结果表明,春性品种出苗率明显高于冬性品种,春性品种平均生育期（91.8 d）比冬性品种(95.0 d)短约3.0 d;春性品种总茎数平均峰值（869.3×10⁴·hm^-2）明显高于冬性品种（772.4×10⁴·hm^-2）;叶面积指数和干物质积累量的平均值在春、冬性品种间差异不明显;新冬41、新春6号、新春43号的产量较高,为7 581.6~7 935.6 kg·hm^-2,获得高产的穗数为431.7×10⁴~515.8× 10⁴·hm^-2,穗粒数 31.6~36.3,千粒重46.8~52.8 g。综合考虑产量、出苗率、成熟期等因素,初步认为新春6号、新冬41号、新春43号适合在北疆超晚播。     

红花双列杂交后代羟基黄色素A含量的遗传效应分析

选用6个羟基红花黄色素A（HSYA）含量差异较大的亲本,采用双列杂交方法配制杂交组合,测定2015年和2016年2年亲本及其后代F₁和F₂红花中的HSYA含量。运用双子叶植物种子数量性状遗传模型和统计分析方法,分析胚、细胞质和母体植株3套遗传体系的基因效应和环境互作效应。结果发现：在HSYA含量的遗传体系中,母体遗传效应影响最大,胚效应次之,细胞质效应影响最小。3套遗传体系均表现出基因主效应大于环境互作效应。机误方差较大,说明HSYA含量还受环境机误或抽样误差的影响。亲本遗传效应分析表明,豫红花1号（P₁）做亲本表现稳定,有利于增加杂交后代HSYA含量,达到提高品质、改良品种的效果。胚显性方差和母体显性方差均达到极显著水平,表明同时存在种子杂种优势和母体杂种优势,而且其主效应基因不受环境影响。综合考虑遗传主效应、胚显性效应和母体显性效应,亲本组合（P₁×P₅）有利于提高后代杂交品种的HSYA含量。该研究结果可为后代材料在杂种优势利用中的亲本选择提供理论支持。     

菌材伴栽与天麻高产栽培技术研究

菌材伴栽与培育栽培天麻法与室外林地常规田字格栽培法、菌材+枝条栽培法、双层菌材栽培法和单层菌材栽培法比较,菌材伴栽法能显著提高天麻产量、生物效益、产值和投入产出比等各项经济技术指标,减少了菌材提前培育过程。该方法实用性强,具有应用和推广价值。     

去势和热应激对筠连黄牛生产性能、瘤胃发酵和血液生化指标的影响

为研究去势和热应激对筠连黄牛生产性能、瘤胃发酵和血液生化指标的影响,本研究选用18头16月龄、体重相近((292.35±28.71) kg)的不同去势程度筠连黄牛(全去势FCG、半去势HCG、假手术SOG),分为3组,每组6个重复,每个重复1头牛,根据试验期间牛舍温湿度指数(THI),分为热应激期(7、8月)和非热应激期(9月),分析去势和热应激对黄牛各指标的影响。结果表明：1)与非热应激期相比,热应激期牛舍THI、肉牛呼吸频率和直肠温度均显著提高(P<0.05)。2)去势和热应激均显著提高血清热休克蛋白70、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)和雌二醇浓度(P<0.05),而睾酮、甲状腺素(T4)、胰岛素和游离脂肪酸浓度显著降低(P<0.05),且除血清葡萄糖、T4和LDL-C外,去势和热应激对其他血清指标存在显著互作效应(P<0.05)。3)全去势和热应激均显著降低肉牛平均日增重(ADG),且存在显著互作效应(P<0.05)。4)热应激期,去势显著降低粗蛋白、酸性洗涤纤维和钙表观消化率以及血清总抗氧化能力(T-AOC)(P<0....     

Effect of dietary vitamin B6 supplementation on growth and intestinal microflora of juvenile golden pompano (Trachinotus ovatus)

In order to investigate the dietary vitamin B₆ (VB₆) requirement for juvenile golden pompano, an experiment of six different diets with six dietary VB₆ levels (0, 3.75, 7.47, 10.6, 13.7 and 18.5 mg/kg) was conducted. The results indicated that the content of dietary VB₆ significantly increased weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR; p < 0.05). Golden pompano fed diet containing 7.47 mg/kg VB₆ achieved the maximum ALT, AST, GPx, hepatic VB₆ concentrations as well as the minimum MDA, however, the ALT, AST, GPx and hepatic VB₆ concentrations decreased while MDA increased with a further increase in dieatry VB₆. The highest values of GR and POD occurred at the 10.6 and 13.7 mg/kg dietary VB₆ (p < 0.05) respectively. A diet supplemented with 7.47 mg/kg VB₆ increased intestinal Na⁺, K⁺‐ATPase, Chymotrypsin, γ‐glutamyl transpeptidase, creatine kinase and amylase activities (p < 0.05). The relative abundance of Tenericutes and Bacteroidetes decreased while Proteobacteria and Firmicutes improved with an increase in dietary VB₆ levels up to 7.47 mg/kg. Quadratic regression analysis on WGR, hepatic VB₆ concentrations, AST and ALT indicated that the optimum dietary VB₆ levels for juvenile golden pompano were 8.84–9.28 mg/kg.