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81.
ZHOU Hua ZHANG Dai-fu SHAN Jiang SU Hai NIU Hui-yan WANG Juan YU Ping QI Wei-gang 《园艺学报》2004,20(12):2261-2264
AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT1 antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced. 相似文献
82.
三肽囊素对环磷酰胺诱导的鸡红细胞免疫功能低下的阻断作用 总被引:3,自引:2,他引:3
本文研究了三肽囊素对环磷酰胺诱导的墟岗黄鸡红细胞免疫功能的抑制模型的影响。180只一日龄墟岗黄母鸡随机分成对照组,三肽囊素组,三肽囊素 环磷酰胺组,环磷酰胺组四个组,三肽囊素组和环磷酰胺 三肽囊素组每只鸡肌注0.01mg/kg剂量的三肽囊素;环磷酰胺组和环磷酰胺 三肽囊素组每只鸡肌注40mg/kg剂量的环磷酰胺,对照组鸡注射等量的生理盐水。结果表明,14日龄和35日龄时各试验组鸡的红细胞总数没有显著差异,而在56日龄时,环磷酰胺组显著低于其它三组(P<0.05);从14日龄开始,环磷酰胺纽的RBC-C3bR花环率显著低于其它三组,而环磷酰胺组的RBC-IC花环率显著高于其它三组,并持续到实验结束。结果提示,三肽囊素可阻断由环磷酰胺诱导的鸡红细胞免疫功能低下。 相似文献
83.
84.
Sato等报道,在质粒载体上引入大肠杆菌Amp^ 抗性基因(内含有6核苷酸片段的ISS免疫增强序列),真皮免疫小鼠后ISS增强CTL和Th1细胞的免疫反应。有人提出利用含有病毒免疫原性基因的基因大片段进行基因免疫,以弥补单基因免疫活性的不足。将协同因子如IL-2、IL-12、TCA等的表达质粒与抗原表达质粒混合注射可使Th1型细胞免疫增强,而GMcsf能明显加强体液免疫, 相似文献
85.
Amino acid profiles of rumen undegradable protein: a comparison between forages including cereal straws and alfalfa and their respective total mixed rations
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B. Wang L. S. Jiang J. X. Liu 《Journal of animal physiology and animal nutrition》2018,102(3):601-610
Optimizing the amino acid (AA) profile of rumen undegradable protein (RUP) can positively affect the amount of milk protein. This study was conducted to improve knowledge regarding the AA profile of rumen undegradable protein from corn stover, rice straw and alfalfa hay as well as the total mixed ratio diets (TMR) based on one of them as forage source [forage‐to‐concentrate ratio of 45:55 (30% of corn stover (CS), 30% of rice straw (RS), 23% of alfalfa hay (AH) and dry matter basis)]. The other ingredients in the three TMR diets were similar. The RUP of all the forages and diets was estimated by incubation for 16 hr in the rumen of three ruminally cannulated lactating cows. All residues were corrected for microbial colonization, which was necessary in determining the AA composition of RUP from feed samples using in situ method. Compared with their original AA composition, the AA pattern of forages and forage‐based diets changed drastically after rumen exposure. In addition, the extent of ruminal degradation of analysed AA was not constant among the forages. The greatest individual AA degradability of alfalfa hay and corn stover was Pro, but was His of rice straw. A remarkable difference was observed between microbial attachment corrected and uncorrected AA profiles of RUP, except for alfalfa hay and His in the three forages and TMR diets. The ruminal AA degradability of cereal straws was altered compared with alfalfa hay but not for the TMR diets. In summary, the AA composition of forages and TMR‐based diets changed significantly after ruminal exposure, indicating that the original AA profiles of the feed cannot represent its AA composition of RUP. The AA profile of RUP and ruminal AA degradability for corn stover and rice straw contributed to missing information in the field. 相似文献
86.
Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin‐like growth factor‐1 gene
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Yang Zhao Yuedong Xuan Daolin Mou Hong Liu Pan Zhou Zhengfeng Fang Lianqiang Che Shengyu Xu Bin Feng Jian Li Xuemei Jiang Yan Lin De Wu 《Journal of animal physiology and animal nutrition》2018,102(5):1340-1350
The study aimed to investigate the effects of maternal dietary methyl donors on the performance of sows and their offspring, and the associated hepatic insulin‐like growth factor‐1 (IGF‐1) expression of the offspring. A total of 24 multiparous sows were randomly fed the control (CON) or the CON diet supplemented with methyl donors (MD) at 3 g/kg betaine, 15 mg/kg folic acid, 400 mg/kg choline and 150 μg/kg VB12, from mating until delivery. After farrowing, sows were fed a common lactation diet through a 28‐days lactation period and six litters per treatment were selected to be fed until at approximately 110 kg BW. Maternal MD supplementation resulted in greater birthweight (p < 0.05) and increased the piglet weights (p < 0.01) and litter weights (p < 0.05) at the age of day 28, compared with that in CON group. The offspring pigs in the MD group had greater ADG (p < 0.05) and tended to lower F:G ratio (p = 0.07) compared with that of CON group from day 28 to 180 of age. The offspring pigs from MD group had greater serum IGF‐1 concentrations and expressions of hepatic IGF‐1 gene and muscular IGF‐1 receptor (IGF‐1r) protein at birth (p < 0.05), and greater hepatic IGF‐1 protein (p = 0.03) and muscular IGF‐1r gene expressions (p < 0.05) at slaughter, than that from the CON group. Moreover, the methylation at the promoter of IGF‐1 gene in the liver of newborn piglets and finishing pigs was greater in the MD group than that of the CON group (p < 0.05). In conclusion, maternal MD supplementation throughout gestation could enhance the birthweight and postnatal growth rate of offspring, associated with an increased expression of the IGF‐1 gene and IGF‐1r, as well as the altered DNA methylation of IGF‐1 gene promotor. 相似文献
87.
乙烯利对干旱胁迫下草地早熟禾生理指标的影响 总被引:1,自引:0,他引:1
以草地早熟禾(Poa pratensis)‘Nuglade'品种为试验材料,研究叶片喷施乙烯利对草地早熟禾在水分充足、干旱及复水恢复3种处理下叶片电解质渗透率、叶绿素、可溶性蛋白、丙二醛(MDA)等生理指标的影响。结果表明,乙烯利与对照组(CK)相比,乙烯利在干旱胁迫下保持更高的叶片相对含水量23.5%(对照为11.5%)和更低的电解质渗透率68.1%(对照为90.7%)及MDA含量53.90μmol·g~(-1)(对照检查131.62μmol·g~(-1)),且复水后的电解质渗透率恢复程度更好;此外乙烯利处理提高了复水恢复过程草地早熟禾的可溶性蛋白含量;说明,叶片喷施乙烯利能够缓解草地早熟禾的干旱胁迫损伤,并促进干旱后的复水恢复。 相似文献
88.
为给我国新型兽医制度建设提供借鉴,对美国国家兽医认证计划(NVAP)及其主要制度进行了研究。研究表明,美国认证兽医是经美国动植物卫生监督局(APHIS)法定程序认证,代表联邦兽医(官)和州动物卫生官(SAHO)实施流通动物检疫监管,协助政府兽医(官)控制动物疫病的执业兽医,具有官方兽医属性。目前,全美80%的兽医为认证兽医,总量高达6.8万名,有效弥补了政府兽医(官)力量的不足。《美国联邦法规》第161.1条规定,NVAP的管理主要涉及认证兽医的分类、职责、培训教育、认证、复审、纪律处分等制度。 相似文献
89.
90.