Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.     

单株幼龄苹果树修剪仿真研究

修剪是果树生产中重要的技术手段,要想研究更加合理的修剪技术体系,就必须保证能够对修剪反应有准确、直观和及时的判断,而仿真模拟技术提供了这种可能。以幼龄苹果树为研究对象,采用不同修剪处理对比的方法,在田间试验的基础上,总结修剪反应规律,构建苹果树结构和修剪模型。在此基础上基于Qt框架和OpenGL图形库,开发了单株苹果树修剪仿真软件平台。结合优化算法,通过仿真平台模拟了给定生产目标的修剪方案优化,优化结果对虚拟学习和实际生产提供了可视化指导。     

Effect of soil matric potential on tomato yield and water use under drip irrigation condition

Field experiment was carried out to investigate the effect of soil matric potential (SMP) on tomato yield, evapotranspiration (ET), water use efficiency (WUE) and irrigation water use efficiency (IWUE) under drip irrigation condition in North China Plain. The experiment included five treatments, which controlled SMP at 0.2 m depth immediately under drip emitter higher than −10 (S1), −20 (S2), −30 (S3), −40 (S4) and −50 kPa (S5), respectively, after tomato plant establishment. The results showed that different SMP affected irrigation amount and tomato ET. Irrigation amount decreased from 185 mm (S1) to 83.6 mm (S5) in 2004, and from 165 mm (S1) to 109 mm (S5) in 2005, respectively. The ET decreased from 270 mm (S1) to 202 mm (S5) in both years. However, it was found that SMP did not affect the tomato yield significantly, for the range of SMP investigated. Both WUE and IWUE increased as SMP decreased. The maximum WUE (253 and 217 kg/ha mm) and IWUE (620 and 406 kg/ha mm) were for S5 in 2 years, whereas the minimum WUE (178 and 155 kg/ha mm) and IWUE 261 and 259 kg/ha mm) were for S1 in 2004 and 2005. Based on the above results, therefore, it is recommended that if the tomatoes are well irrigated (SMP is higher than −20 kPa) during establishment, controlling SMP higher than −50 kPa at 0.2 m depth immediately under drip emitter can be used as an indicator for drip irrigation scheduling during following period of tomato growth in North China Plain.     

Effect of water deficit in different growth stages on stem sap flux of greenhouse grown pear-jujube tree

The effects of water deficit in different fruit growth stages on the variation of stem sap flux of 6-year old greenhouse-grown pear-jujube trees were investigated. Treatments included sufficient water supply during the whole fruit-growing period (T₁), mild water deficit during the flowering–fruit setting stage (T₂), moderate water deficit during the fruit rapid growth stage (T₃) and severe water deficit during the fruit maturing stage (T₄). Results showed that significant compensation effect on stem sap flux after re-watering was observed in T₂, but not in T₃ and T₄ stages. At the end of rapid growth stage, the diurnal variation of stomatal conductance generally had a similar trend as that of stem sap flux, but with a distinct midday depression from 12:00 to 14:00 p.m. In addition, a linear relationship between the relative available soil water content (RAWC) and the ratio of daily stem sap flux to that of sufficient water treatment was observed (R² = 0.4489).     

Drip irrigation scheduling for tomatoes in unheated greenhouses

During the last two decades, energy-saving solar greenhouses without heating systems, about 150-800 m² (width: 5-10 m, length: 30-80 m) in size, have been widely used in the North of China for vegetable production during cold seasons. The greenhouse is also suitable for use in other countries located in the temperate or warm-temperate zones, especially in the developing countries, because it is inexpensive with a simple structure, and does not need any additional energy for heating in winter. Drip irrigation has been recognized as a sensible method of supplying water inside the greenhouses, and a simple method for drip irrigation scheduling at low cost is urgently required. Using the water-balance method, the authors studied the water consumption of tomato (Lycopersicon esculentum Mill.) and the relationship between evapotranspiration and water surface evaporation measured with 20 cm pan. Research results show that there is no significant water flux at 0.75 m depth when soil water potential is kept higher than -20 kPa at 15 cm depth, and around -20 kPa at 60 cm depth. The accumulative value of evapotranspiration is approximately equal to the accumulative value of water surface evaporation measured using 20 cm pan. Further analysis found that 20 cm pan can be used to determine the water requirement of tomato drip irrigation scheduling in this type of greenhouse.     

临界温度下循环喷油量对柴油机冷起动和排放特性的影响

为探明循环喷油量对小型农用柴油机冷起动特性和排放性能的影响规律,同时寻求其临界温度下的最佳循环喷油量,该研究以一款直列双缸高压共轨柴油发动机为研究对象,利用环境模拟舱整机试验平台,通过多次预试验测得海拔2000 m下,该机不开启预热装置能够成功起动的临界温度为-5 ℃。在此工况下,采用3次喷射方式,通过改变主喷油量研究循环喷油量对该机冷起动特性和排放性能的影响。结果表明：较大或较小的循环喷油量均会导致柴油机冷起动时间增加,甚至起动失败;随着循环喷油量增大,冷起动时间先减小后增加,循环喷油量为20 mg时冷起动时间最短,且HC、CO排放峰值均最低,分别为12984和3008 mg/L;增大或减少循环喷油量均会导致HC和CO排放峰值增加。循环喷油量较大时,CO排放峰值显著增加,并且峰值出现时刻提前。改变循环喷油量对NOx排放峰值有较大影响,循环喷油量为30 mg时NOx排放峰值最小,为368 mg/L。综合考虑,海拔高度2 000 m,环境温度-5 ℃工况下该柴油机起动的最佳循环喷油量为20 mg。研究结果可为柴油机冷起动特性和排放优化提供支撑。     

金钟藤在海南的危害与防治

对国内原产地的金钟藤进行初步调查,发现该种在海南岛分布广泛,并对该岛中部山区的中低海拔次生林和人工残次林造成严重危害;而在云南、广西等省区虽有分布,但危害较轻。根据对金钟藤生物生态学特性的分析,结合其在新旧分布地的现状,认为该种为典型的生态危害种,具有很强的潜在入侵能力,对其防治应以综合防治为主。     

沙棘植物柔性坝坝体变形初步分析

本文基于2005年在内蒙古准格尔旗西召沟小流域对已植的沙棘柔性坝进行野外调查,从植物生长角度对沙棘柔性坝坝体进行了纵向、横向、垂向(即沙棘柔性坝坝长、坝宽、坝高)的变形分析。对于沙棘柔性坝的坝长变化,主要从沙棘根系蘖苗萌生出发,分析得出其每年扩展约1m;柔性坝坝宽受沟宽限制,其发展趋势为扩展至满沟;柔性坝坝高变化采用三次函数和Logistic型函数两种具有“S”形特征的曲线进行拟合分析,经对比其变化过程服从Logistic型生长函数,并结合Logistic函数对沙棘植物柔性坝坝高的变化进行了具体意义的探讨。