农杆菌介导的马铃薯茎段遗传转化体系优化研究

以甘肃主栽品种的茎段为外植体,研究了不同转化条件(菌液浓度、感染时间、预培养及共培养时间)对转化效率的影响,以及选择压和培养基对抗性出芽的影响,初步建立了马铃薯品种“陇薯3号”和“台湾红皮”的遗传转化体系并获得了转化植株。卡那霉素生根筛选和PCR鉴定结果表明,再生的转化植株假阳性率比较高,要获得预期的转基因植株群体需加大遗传转化工作中转基因植株的数量。     

Measurements to Protect Owners'' Benefits under the Non-price Bottom Bidding

Investment on construction projects has become an important factor in improving the development of economy in China.The non-price bottom bidding is a representation that the investment on projects is becoming standardized.During the years of trying out the non-price bottom bidding,the colluding in bidding or bidding below the cost has happened several times,which have damaged the benefits of owners.To solve theses problems,this paper firstly studies the reason of these problems-the failure in the normal relationship between principal and agent.Then,some measures have been presented as follows: building systematic and scientific system of qualification prejudication,judging behaviors of colluding in bidding and bidding below the cost with reason and providing a surety mechanism of the project.The authors hope that these measures are beneficial to non-price bottom bidding.     

Strength and Microstructure of Sulphoaluminate Cement Containing Ultra- Fine CaCO3

The existence of ettringite in sulphoaluminate cement hardened paste is mainly in the form of pole-like crystal,which causes unfavorable effect on the cement.In this paper the strength and microstructure of the sulphoaluminate cement containing ultra-fine CaCO_3 weze discussed.Experimet results show that the strength of sulphoaluminate cement was obviously improved by adding 3% of ultra-fine CaCO_3.After curing for 28d,the compressive strength of cement paste and mortar is 100.6MPa and 94.1MPa respectively,and the bending strength of the mortar reached 12.5MPa.SEM study on hardened paste of sulphoaluminate cement containing ultra-fine CaCO_3 showed that its structure was dense and homogeneous,there was hardly any massive crystall detected.     

Analysis on Longitudinal Cracks and Deflection of the Pre-stressed V-shape Slab Roof Structure

In this paper, the finite element method is used to analyze the behavior of the prestressed V-shape Slab Roof structure, especially the effect of temperature change on the stress of slab. The calculated results indicate that when the temperature changes, the tensile stress at the lower extreme fibers of the flange plate near rib exceeds the tensile strength of concrete subjected to the combined action of triaxial stresses and results in the longitudinal cracks of the flange plate. The deflection analysis is based on the mechanical model of the prestressed V shape slab with the longitudinal cracks. The calculated results are in general agreement with the field observations.     

Determination to Weighting Coefficient of Combination Forecast Based on Rough Set Theory

The problem of determination to weighting coefficient is a key and difficulty for combination forecast. A method of determining weighting coefficient based on rough set theory is showed in this paper. Determining weighting coefficient is translated into estimating significance of attributes among rough set. A relation data model about combination forecast is established. Knowledge systems are built through making attribute value into eigenvalue. Under data moving, the weighting coefficients of a combination forecast model are computed by analyzing the dependence and significance of forecasting method for the predicted object. The proposed approach overcomes the subjectivity of traditional determination to weighting coefficient, avoids computing linear or nonlinear extremum problem and makes combination forecast more objective. The validity of the proposed approach is verified with a case.     

观赏羽衣甘蓝的离体繁殖

以花蕾为外植体，离体繁殖羽衣甘蓝８个杂各一代材料。培养基为（ｍｇ／Ｌ）：ＭＳ＋ＢＡ１－２＋ＮＡＡ０．１－０．２＋ＩＡＡ０．５＋（或不加）ＧＡ３０．３＋３％蔗糖。平均１８％－６７％的接咎花蕾产生不定芽；药有３０％的不定芽发育成完整植物。培养物的严重褐化和再生植物的普遍玻璃质化是影响植株成活的主要因素。培养基中加入活性炭和青霉素，并给以强光照射，可提高植株成活率。     

多效唑在小麦花药培养中应用的研究

在小麦花粉愈伤诱导及分化培养基中,加入一种三唑类化介物---多效唑(MET),对出愈率和绿苗分化率影响不明显,但当其浓度高于2mg/L时,会出现负效应。在小麦壮苗培养基中附加MET3 mg/L,萘乙酸(NAA)0.5mg/L,蔗糖8％,不仅可改善春性小麦花粉植株的生长状态,还可提高花粉苗染色体加倍率。若与将愈伤转入分化培养基以前先在含秋水仙碱250mg/L的MS培养基中处理1天这一方法相配合使用,将会出现更好的加倍效果。冬性小麦花粉植株的壮苗培养基中添加MET,可有效地防止花粉苗在越夏过程中老化,增加分蘖数,从而大幅度提高移栽成活率,但同时花粉植株的染色体加倍率有所下降。     

生物质气体燃料概述与展望

论述了生物质气体燃料的种类以及生产各种气体燃料的相应技术及其国内外发展概况和存在问题.生物质气化方面国内外有产业化实例;生物发酵制氢大多数处于实验阶段,关键是培育高效产氢发酵菌种、进一步提高系统的产氢能力、降低生产成本;厌氧发酵制取沼气生产技术比较成熟,在其生产过程中不消耗其它能源、没有环境污染、而且可降解各种有机废弃物,是目前最有希望的生物质能源之一.     

嫁接苗盘自动叠放装置研究与设计

为了提高嫁接作业的自动化水平,实现嫁接操作的流水线生产,设计了用于嫁接作业后期对载有嫁接苗的苗盘自动叠放的装置。该装置的机构部分包括由滚筒和线性模组构成的推送平台,以及由链条、链轮、机架构成的升降机构,采用PLC进行系统控制。试验表明,该装置运行稳定可靠,升降定位精度±1.0mm,最长动作周期26s,适用于现阶段蔬菜嫁接生产流水线。     

提高渠系水有效利用系数的节水效果计算

提高渠系水有效利用系数能否节约水资源取决于渠系渗漏的水是否都转化成了被重新利用的地下水。本文建立了提高渠系利用系数的节水效果的计算公式，并进行了理论分析和实例计算。结果表明，虽然提高渠系利用系数的节水效果没有原来不考虑渠系渗漏水重新利用时简单估计的大，但仍然是十分明显的