The protein composition of sheep foetal fluids

Amniotic fluid contains several proteins including alpha-foetoprotein derived from the foetal plasma, but lacks the higher molecular weight alpha2- and gamma-globulins. The protein composition of the fluid, like that of the serum, remains unchanged after the first month of gestation, and is buffered from the influence of the allantoic fluid by the amnion. Allantoic fluid contains a significantly higher concentration and a greater variety of proteins; it is not a simple transudate of either foetal or maternal plasma, and though containing proteins derived from both sources, it is deficient in albumin but relatively abundant in gamma-globulins. The latter comprises gamma-foetoprotein and mature type IgG. It is believed that maternal IgG may reach the allantoic sac in appreciable amount, but its transmission to the amniotic sac and thence to the foetus may be prevented by the impermeability of the amnion.     

Longevity of colonies of Anaplasma marginale in midgut epithelial cells of Dermacentor andersoni

Colonies of Anaplasma marginale in midgut epithelial cells of experimentally infected Dermacentor andersoni were studied in adult ticks 1, 3, and 6 months old. Longevity of the parasite in ticks was assessed by evaluating its infectivity for splenectomized calves; calves were exposed by feeding ticks and by inoculation of tick gut homogenates. Longevity was also evaluated by determining size, type, and density of colonies in male and female ticks. The effect of incubation (2.5 days at 37 C) on colony density was also examined for ticks at each age period. All methods used to assess longevity of A marginale in ticks (tick transmission, calf inoculation, and histologic studies) indicated a decrease of the numbers of organisms in 6-month-old ticks. Furthermore, when tick gut homogenates from 6-month-old nonincubated ticks were not infectious for susceptible calves, incubation of ticks before dissection restored infectivity of homogenates. Colonies of A marginale were detected in gut tissues of 6-month-old ticks that were not infective; therefore, infectivity of ticks could not be confirmed merely by presence of A marginale colonies.     

Method for obtaining ovine uterine secretions from unilaterally pregnant ewes

Development of the ovine conceptus was confined to the uterine horn ipsilateral to the corpus luteum (CL) by placing a ligature around that uterine horn at a point near the uterine body on day 5 of pregnancy. On day 140 of gestation, seven of 10 ewes were still pregnant and from 21 to 815 ml of uterine fluid (488 +/- 94 ml, X +/- SEM) were collected from the nongravid uterine horn. Total recoverable protein (X +/- SEM) was 13.4 +/- 3.4 grams. Polyacrylamide gel electrophoresis of the reduced proteins in presence of sodium dodecyl sulfate indicated that protein composition of uterine fluid was distinct from that of colostrum, serum, amniotic fluid, and allantoic fluid, and revealed the presence of two major polypeptides with molecular weights of about 57,000 and 58,500, respectively, plus numerous other minor components. Gel filtration on columns of Sephadex G-200 and Sepharose CL-6B suggested that these polypeptides formed a series of aggregates of high molecular weight when kept under nonreducing conditions. Glucose (.18 +/- .03 mg/ml), but not fructose, was present in uterine fluid. In addition, high levels of prostaglandin F (451.4 +/- 83.3 ng/ml) were present.     

Quantifying potato pathogen DNA in soil

An improved method for the direct extraction of DNA from soil involving processing of a relatively large sample (60 g) was developed. The accurate and reliable detection and quantification of the soil-borne potato pathogens Colletotrichum coccodes (black dot), Rhizoctonia solani (black scurf) and Spongospora subterranea (powdery scab) following inoculation of soils was demonstrated. With this method, low levels of target DNA (30–40 pg DNA/g soil) could be detected in field soils. DNA recovery was proportionate across a wide range of inoculum (R² > 0.86) and there was no effect of soil type on the recovery of C. coccodes. The method was used to assess levels of naturally occurring pathogen DNA in 122 soil samples obtained from commercial potato fields.     

Assessment of retinal recognition technology as a biometric method for sheep identification

In order to assure effective traceability, food-producing animals must be identified by a tamper-proof and durable technique. With the advance in human biometric technologies, the deployment of retinal recognition technology for cattle identification and verification has been prompted. The objective of this study was to assess the accuracy of a commercially available retina biometric technology for sheep identification (i) by determining whether light conditions during retinal image capture (indoors and outdoors with shade) and different operators exerted any significant effect on the matching score of the built-in pattern matching algorithm; and (ii) by evaluating the recognition performance of the biometric system for enrolment of one retinal image per sheep and two retinal images per sheep (bimodal biometric system). Neither the light conditions nor the operators were found to have a statistically significant effect on the matching score values of the built-in algorithm; yet it was clear that the pupillary light reflex phenomenon played a major role in obtaining lower matching score values for retinal images taken outdoors. The recognition errors of the one-retina biometric system were estimated to be 0.25% for false matches and 0.82% for false non-matches. An improved bimodal biometric system, i.e., two retinas, that applies a decision criterion based on a simple OR logical operator and a sum of matching scores, has been proposed in this study in order to reduce both probabilities of false matches and false non-matches to near zero.     

Prediction of relative tissue proportions in wheat mill streams by fourier transform mid-infrared spectroscopy

Fourier-transform mid-infrared (FTIR) spectroscopy was investigated as a method to quantify the relative wheat grain tissue proportion in milling fractions. Spectra were acquired with a FTIR spectrometer equipped with an attenuated total reflectance device on ground samples, and the relative tissue proportion was determined according to the biochemical marker methodology as the reference method. Partial least-squares models were developed independently to predict the amount of outer pericarp, aleurone layer, starchy endosperm, and an intermediate layer (made up of inner pericarp plus seed coat plus nucellar epidermis). Good quality of prediction was obtained regardless of the target tissue. The standard errors of prediction obtained for the outer pericarp, intermediate layer, aleurone layer, and starchy endosperm quantification were, respectively, 3.4, 1.3, 3.4, and 4.6%.     

Conservation farming strategies in East and Southern Africa: Yields and rain water productivity from on-farm action research

Improved agricultural productivity using conservation farming (CF) systems based on non-inversion tillage methods, have predominantly originated from farming systems in sub-humid to humid regions where water is not a key limiting factor for crop growth. This paper presents evidence of increased yields and improved water productivity using conservation farming in semi-arid and dry sub-humid locations in Ethiopia, Kenya, Tanzania and Zambia. Results are based on on-farm farmer and research managed experiments during the period 1999–2003. Grain yield of maize (Zea mays L.) and tef (Eragrostis Tef (Zucc)) from conventional (inversion) tillage are compared with CF with and without fertilizer. Rain water productivity (WP_rain) is assessed for the locations, treatments and seasons. Results indicate significantly higher yields (p < 0.05) for CF+ fertilizer treatments over conventional treatments in most locations, increasing from 1.2 to 2 t ha^?1 with 20–120% for maize. For tef in Ethiopian locations, the yield gains nearly doubled from 0.5–0.7 to 1.1 t ha^?1 for “best bet” CF+ fertilizer. WP_rain improved for CF+ fertilizer treatments with WP gains of 4500–6500 m³ rainwater per t maize grain yield in the lower yield range from 0 to 2.5 t ha^?1. This is explained by the large current unproductive water losses in the on-farm water balance. There was a tendency of improved WP_rain in drier locations, which can be explained by the water harvesting effect obtained in the CF treatments. The experiences from East and Southern Africa presented in this paper indicate that for smallholder farmers in savannah agro-ecosystems, conservation farming first and foremost constitutes a water harvesting strategy. It is thus a non-inversion tillage strategy for in situ moisture conservation, rather than solely aimed at minimum tillage with mulch cover. Challenges for the future adoption of CF in sub-Saharan Africa include how to improve farmer awareness of CF benefits, and how to efficiently incorporate green manure/cover crops and manage weeds.     

Deposition of cell wall polysaccharides in wheat endosperm during grain development: Fourier transform-infrared microspectroscopy study

The time course and pattern deposition of the cell wall polysaccharides in the starchy endosperm of wheat (Triticum aestivum cv. Recital) during grain development was studied using Fourier transform infrared microspectroscopy (micro-FTIR). Three stages of grain development identified as key stages for cell wall construction were retained as follows: the end cellularization, the beginning of cell differentiation, and the beginning of maturation. Micro-FTIR revealed that beta-(1-->3),(1-->4) glucans and arabinoglactan proteins are the main cell wall components of endosperm at the end of the cellularization stage, whereas arabinoxylans (AX) appeared only at the cell differentiation stage. Past the differentiation stage, FTIR spectra were dominated by AX features. Cell walls at the beginning of cell differentiation and at endosperm maturation could be distinguished by spectral features that were ascribed to AX substitution. AX appeared more substituted at the beginning of cell differentiation. Moreover, a difference in the degree of AX substitution was found between peripheral and central parts of the grain at the cell differentiation stage; AX in central cells was less substituted. Thus, dramatic changes in endosperm cell wall composition were detected during wheat grain development with respect to both the relative occurrence of individual constituents and the fine structure of the AX.