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排序方式: 共有178条查询结果,搜索用时 15 毫秒
1.
Summary All accessions tested of four cultivated species ofCucurbita responded to seedling treatment with 250 ppm 2-chloroethylphosphonic acid by developing only pistillate flowers for an extended period. This growth regulator is a promising means of facilitating hybrid seed production of squash. A similar treatment did not induce flowering of wild species ofCucurbita that were vegetative under the environmental conditions of the experiment.Approved by the Director of the New York State Agricultural Experiment Station for publication as Journal Paper No. 1737. 相似文献
2.
Mannheimia granulomatis PCR检测方法的建立 总被引:3,自引:0,他引:3
Mannheimia granulomatis是导致牛、羊呼吸道传染病的一种病原菌.为快速、准确诊断由该菌导致的疫病,从基因库中获得M.granulomdtis的基因序列,再从M.granulomatis的基因序列中获得与其他细菌包括溶血性曼氏杆菌和多杀性巴氏杆菌等不同的基因片段,设计引物,建立了特异性好、敏感性较高的M.granulomatis的PCR诊断方法.结果表明,其特异性为100%,最小检出量为5×104cfu/mL~5×103cfu/mL M.granulomatis或1/10个单个菌落. 相似文献
3.
Janice K Gard William C Hutton Jeanette A Baker RK Singh Paul CC Feng 《Pest management science》1999,55(2):215-217
While the use of NMR and stable isotopes in metabolism studies is hardly new, it is only recently that isotope-edited NMR spectroscopy has been applied in kinetic studies of glyphosate metabolism of soil microbes. NMR can detect multiple species simultaneously and non-destructively, yielding valuable information on structural identification of metabolites. T riple R esonance I sotope ED ited spectroscopy (TRIED), [2H]NMR, and [2H–13C] INEPT (I nsensitive N ucleus E nhancement through P olarization T ransfer) are three isotope-edited techniques which have been used in combination to examine the microbial degradation of glyphosate (N-phosphonomethylglycine). Using 13C- and 15N-labeled glyphosate, TRIED can detect multiple metabolites in crude matrices at submicrogram levels, an improvement over earlier techniques where milligrams were needed. It can detect 500 nanograms of 13C–15N-labeled compound in a crude sample (1 : 1400 mass ratio), only a few hours work being required. [2H]NMR and [2H–13C]INEPT were also used as complementary techniques to further examine metabolites whose 13C–15N bond has been cleaved. The three-isotope-edited methods produced results consistent with both radioactivity and HPLC analyses. Accordingly, we are able to detect minute levels of metabolites in the presence of complex mixtures, minimizing the costs and time of sample purification. ©1999 Society of Chemical Industry 相似文献
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Whitaker BD Schmidt WF Kirk MC Barnes S 《Journal of agricultural and food chemistry》2001,49(8):3787-3792
Hexane extracts of epicuticular wax from cv. Gala apples were noted to have an unusual, broad absorbance maximum at approximately 258 nm, which led us to isolate and identify the primary UV-absorbing compounds. Column and thin-layer chromatography yielded a fraction that gave a series of paired, 260-nm-absorbing peaks on C(18) HPLC. These were shown to be a family of phenolic fatty acid esters, for which retention times increased with increasing fatty acid chain length, and paired peaks were esters of two related phenolics with the same fatty acid moiety. Alkaline hydrolysis of the esters released two water-soluble phenolics separable by C(18) HPLC. Electrospray ionization mass spectrometry gave a molecular mass of 150 for both, and (1)H NMR plus UV absorbance spectra identified them as E and Z isomers of p-coumaryl alcohol. Alkaline cleavage of the fatty acid esters in the presence of methanol or ethanol resulted in partial derivatization of E-p-coumaryl alcohol to the corresponding gamma-O-methyl or O-ethyl ether. Gradient HMQC NMR of the HPLC-purified stearate ester of E-p-coumaryl alcohol indicated that fatty acid esterification occurs at the gamma-OH rather than at the 4-OH on the phenyl ring. This is the first report of fatty acid esters of monolignols as a natural plant product. 相似文献
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J. L. Snider G. D. Collins J. Whitaker C. D. Perry D. R. Chastain 《Journal of Agronomy and Crop Science》2014,200(1):77-82
Despite exhaustive literature describing drought stress effects on photosynthesis in Gossypium hirsutum, the sensitivity of photosynthetic electron flow to water deficit is heavily debated. To address this, G. hirsutum plants were grown at a field site near Camilla, GA under contrasting irrigation regimes, and pre‐dawn water potential (ΨPD), stomatal conductance (gs), net photosynthesis (PN), actual quantum yield of photosystem II (ΦPSII) and electron transport rate (ETR) were measured at multiple times during the 2012 growing season. ΨPD values ranged from ?0.3 to ?1.1 MPa. Stomatal conductance exhibited a strong (r2 = 0.697), sigmoidal response to ΨPD, where gs was ≤0.1 mol m?2 s?1 at ΨPD values ≤ ?0.86 MPa. Neither ΦPSII (r2 = 0.015) nor ETR (r2 = 0.010) was affected by ΨPD, despite exceptionally low ΨPD values (?1.1 MPa) causing a 71.7 % decline in PN relative to values predicted for well‐watered G. hirsutum leaves at ΨPD = ?0.3 MPa. Further, PN was strongly influenced by gs, whereas ETR and ΦPSII were not. We conclude that photosynthetic electron flow through photosystem II is insensitive to water deficit in field‐grown G. hirsutum. 相似文献
9.
C. Jacyn Baker Daniel P. Roberts Norton M. Mock Bruce D. Whitaker Kenneth L. Deahl Andrey A. Averyanov 《Physiological and Molecular Plant Pathology》2005,67(6):1322
The plant apoplast is an important mediator of communication between the cell cytoplasm and its surroundings. Plant cell suspensions offer a convenient model system to gain insight into apoplastic physiology. Here, we describe a novel phenomenon that took place when two naturally occurring phenolics were added together to either soybean or tobacco cell suspensions. Acetosyringone (AS) and/or hydroxyacetophenone (HAP), phenolics found in the extracellular/apoplast of tobacco cells, were added to soybean or tobacco cell suspensions undergoing an oxidative burst. Individually, AS appeared to be utilized as a typical peroxidase substrate to scavenge hydrogen peroxide, while HAP was utilized at a much lower rate. However, when added together the rate of utilization of both phenolics increased and surprisingly resulted in the production of hydrogen peroxide. We have further characterized this novel phenomenon in suspension cells. This study demonstrates that certain phenolics in plants can cause co-oxidation which, as in animals, could alter the structure and bioactivity of surrounding phenolics. 相似文献
10.
Duarte-Vázquez MA García-Almendárez BE Regalado C Whitaker JR 《Journal of agricultural and food chemistry》2001,49(9):4450-4456
A neutral peroxidase isozyme (pI 7.2) from turnip roots (TNP) was purified to homogeneity and partially characterized. TNP is a monomeric glycoprotein with 9.1% carbohydrate content and a molecular weight of 36 kDa. Optimum pH values for activity using 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and guaiacol as H donors were 4.5 and 5.5, whereas the K(m) values were 0.7 and 3.7 mM, respectively. The ABTS K(m) was approximately 7 times higher than that reported for basic commercial horseradish peroxidase (HRP-C). TNP retained approximately 70% activity after 11 min of heating at 65 degrees C, whereas the activation energy for inactivation (132 kJ/mol) was higher than or comparable to that of other peroxidases. The low ABTS K(m) and high specific activity (1930 units/mg) gave a high catalytic efficiency (500 M(-1) s(-1)). These properties make TNP an enzyme with a high potential as an alternative to HRP in various applications. 相似文献