Risk factors associated with sero-positivity to Toxoplasma gondii in captive neotropical felids from Brazil

From September 1995 to February 2001, blood samples were collected from 865 neotropical felids belonging to 8 different species. These animals were housed in 86 institutions located in 78 cities of 20 Brazilian states. Our goal was to identify the risk factors associated with sero-positivity to Toxoplasma gondii in captive neotropical felids from Brazil. All serum samples were tested by the modified agglutination test (MAT), using formalin-fixed whole tachyzoites and mercaptoethanol. For each animal an individual questionnaire was filled with questions about tattoo number, felid species, age, sex, origin, number of animals in the group, introduction of new animals in the group, time in the institution, eating meat previously frozen for a period <7 days in the last 6 months, eating meat of run-over or euthanized animals in the last 6 months, predation of rodents or birds in the last 6 months and presence of domestic cats near the enclosures in the last 6 months. The total sero-prevalence was 55% (95% CI: 52%, 57%). We estimated a prevalence of 46% (95% CI: 40%, 54%) for jaguarundi (Puma yagouaroundi); 58% (95% CI: 53%, 63%) for ocelot (Leopardus pardalis); 50% (95% CI: 45%, 56%) for oncilla (L. tigrinus); 54% (95% CI: 46%, 62%) for margay (L. wiedii); 12% (95% CI: 4%, 31%) for Pampas-cat (L. colocolo); 83% (95% CI: 65%, 93%) for Geoffroy's-cat (L. geoffroyi); 64% (95% CI: 50%, 68%) for jaguar (Panthera onca) and 48% (95% CI: 42%, 54%) for puma (Puma concolor). Multiple logistic regression was used to evaluate the association between the variables in the questionnaire and sero-positivity to T. gondii. We concluded that the independent risk factors for toxoplasmosis were: age >3 years (OR = 4.75 [2.75; 8.2]), eating meat previously frozen for a period <7 days (OR = 2.23 [1.24; 4.01]), and consumption of animals that were run-over or euthanized (OR = 1.64; [1.14; 2.37]).     

Can carbon sequestration markets benefit low-income producers in semi-arid Africa? Potentials and challenges

The Clean Development Mechanism (CDM) of the Kyoto Protocol of the United Nations Framework Convention on Climate Change allows a country that emits C above agreed-upon limits to purchase C offsets from an entity that uses biological means to absorb or reduce greenhouse emissions. The CDM is currently offered for afforestation and reforestation projects, but may apply subsequently to sequestration in agricultural soils. Additionally, markets outside of the Protocol are developing for soil C sequestration.     

Euthanasia of honey-bee colonies: Proposal of a standard method

Honey bees are most often kept for production purposes. Sanitary, regulatory, or zootechnical circumstances may lead the beekeeper or the veterinarian to dispose of a honey-bee colony. Unfortunately and at present, no standard method of euthanasia exists, leaving the door open to many more or less acceptable practices. Based on a short survey of current practices in 8 countries, we list and rank these methods. Although imperfect, the sulfur dioxide technique appears to be the most efficient. We suggest that it should become the reference method to be taught and incorporated into veterinary and regulatory guidelines.     

Path analysis of spatial predictors of front-yard landscape in an anthropogenic environment

Contagious spatial patterns were shown to exist in the landscape of front-yards in street sections of Hochelaga-Maisonneuve, Montréal. Neighbour mimicry was hypothesized as the mechanism behind this pattern (Zmyslony and Gagnon 1998). To assess the role of spatial environmental factors in structuring this pattern, we carried out a path analysis on the front-yard landscape with five spatial factors: relative distance, street side, width, depth and type of front-yard. We removed all non-significant factors from our model with simple Mantel tests and untangled the common spatial component from the relationship between spatial factors and front-yard landscape with partial Mantel tests. We then used path analysis to evaluate the relative importance of all significant spatial factors in structuring front-yard landscape and to determine the r ² (% of landscape variation explained by spatial factors). Results showed that (1) among all spatial environmental factors, distance (proximity) remained the best predictor of front-yard vegetation – distance alone explained an average of 20% of the landscape variation of a street section, (2) depth, width and type of front-yard also structured the front-yard landscape independently of distance, (3) front-yard landscape expresses greater similarity within the same side of a street section, and (4) in two street sections of Hochelaga-Maisonneuve, spatial factors predicted over 40% of the landscape variation. This suggests (1) that landscape contagion exists also in highly humanized environments and (2) that the mimicry phenomenon was induced not only by proximity, but also by similar environmental conditions in same side street sections and whole street sections. Finally, we suggest that street sections are a very useful and appropriate unit of analysis of urban ecosystems.     

Vectorizing agrochemicals: enhancing bioavailability via carrier‐mediated transport

Systemicity of agrochemicals is an advantageous property for controlling phloem sucking insects, as well as pathogens and pests not accessible to contact products. After the penetration of the cuticle, the plasma membrane constitutes the main barrier to the entry of an agrochemical into the sap flow. The current strategy for developing systemic agrochemicals is to optimize the physicochemical properties of the molecules so that they can cross the plasma membrane by simple diffusion or ion trapping mechanisms. The main problem with current systemic compounds is that they move everywhere within the plant, and this non‐controlled mobility results in the contamination of the plant parts consumed by vertebrates and pollinators. To achieve the site‐targeted distribution of agrochemicals, a carrier‐mediated propesticide strategy is proposed in this review. After conjugating a non‐systemic agrochemical with a nutrient (α‐amino acids or sugars), the resulting conjugate may be actively transported across the plasma membrane by nutrient‐specific carriers. By applying this strategy, non‐systemic active ingredients are expected to be delivered into the target organs of young plants, thus avoiding or minimizing subsequent undesirable redistribution. The development of this innovative strategy presents many challenges, but opens up a wide range of exciting possibilities. © 2018 Society of Chemical Industry     

Effects of substrate sterilization,fungicide treatment,and mycorrhization helper bacteria on ectomycorrhizal formation of pedunculate oak (Quercus robur) inoculated with Laccaria laccata in two peat bare-root nurseries

Summary Pedunculate oak seedlings (Quercus robur) inoculated with the ectomycorrhizal fungus Laccaria lacata were grown for 1 year on fertilized sphagnum peat in two nurseries. Three factors affecting microbial populations in the substrate were studied, fungicide treatment of the seeds, peat disinfection before sowing (methyl bromide or steam pasteurization), and inoculation with mycorrhization helper bacteria. Treatment of acorns with Iprodione had no depressive effect on mycorrhiza formation. Both disinfection techniques were equivalent, stimulating or depressing mycorrhiza formation depending on the initial microflora in the peat. The introduction of two previously selected mycorrhization helper bacteria (one Pseudomonas fluorescens and one unidentified fluorescent pseudomonad), isolated from L. laccata sporocarps associated with Douglas fir—L. laccata ectomycorrhizas in other nurseries, significantly increased the mycorrhizal rate from 30 to 53% of the short roots. The implications of these results for the controlled mycorrhization of planting stocks and the specificity of mycorrhization helper bacteria are discussed.     

Fine-earth translocation by tillage in stony soils in the Guadalentin, south-east Spain: an investigation using caesium-134

Tillage erosion is increasingly recognised as an important soil erosion process on agricultural land. In view of its potential significance, there is a clear need to broaden the experimental database for the magnitude of tillage erosion to include a range of tillage implements and agricultural environments. The study discussed in this paper sought to address the need for such data by examining tillage erosion by a duckfoot chisel plough in stony soils on steep slopes in a semi-arid environment. Results of the investigation of coarse fraction (rock fragment) translocation by tillage in this environment have been presented elsewhere and the paper focuses on tillage translocation and erosion of the fine earth. Tillage translocation was measured at 10 sites, representing both upslope and downslope tillage by a duckfoot chisel plough on five different slopes, with tangents ranging from 0.02 to 0.41. A fine-earth tracer, comprising fine earth labelled with ¹³⁴Cs, was introduced into the plough layer before tillage. After a single pass of the plough, incremental samples of plough soil were excavated and sieved to separate the fine earth from the rock fragments. Translocation of the fine-earth tracer was established by analysing the ¹³⁴Cs content of the samples of fine earth. These data were used to establish translocation distances for each combination of slope and tillage direction. Translocation distances of the fine earth were not significantly different from translocation distances of the coarse fraction. For all sites, except uphill on the 0.41 slope, translocation distances were found to be linearly related to slope tangent. The soil flux due to tillage for each site was calculated using the translocation distance and the mass per unit area of the plough layer. For slopes with tangents <0.25, the relationship between soil flux and tangent was linear and the soil flux coefficient derived was 520–660 kg m⁻¹ per pass. This is much larger than the coefficients found in other studies and this high magnitude is attributed to the non-cohesive nature and high rock fragment content of the soil in this investigation. A second contrast with previous studies was found in non-linearity in the relationship between soil flux and tangent when steeper slopes were included. This was a product of variation in plough depth between the steepest slopes and the remainder of the study area. On the basis of the study it is suggested that an improved understanding of tillage erosion may be obtained by considering the dual processes of tillage detachment (mass per unit area of soil subject to tillage) and tillage displacement (equivalent to translocation distance per pass) in assessing, comparing and modelling tillage translocation. An improved model is proposed that recognises the complexity of soil redistribution by tillage, provides a framework for process-based investigation of the controls on tillage fluxes, and allows identification of potential self-limiting conditions for tillage erosion.     

Impact of xylanases with different substrate selectivity on gluten-starch separation of wheat flour

The influence on wheat flour gluten-starch separation of a xylanase from Aspergillus aculeatus (XAA) with hydrolysis selectivity toward water extractable arabinoxylan (WE-AX) and that is not inhibited by wheat flour xylanase inhibitors was compared to that of a xylanase from Bacillus subtilis (XBS) with hydrolysis selectivity toward water unextractable arabinoxylan (WU-AX) and that is inhibited by such inhibitors. XAA improved gluten agglomeration through degradation of WE-AX and concomitant reduction in viscosity, which in the laboratory scale batter procedure with a set of vibrating sieves (400, 250, and 125 microm), increased protein recoveries on the 400 microm sieve. In contrast, XBS had a negative effect as it decreased gluten protein recovery on this sieve, probably as a result of the viscosity increase that accompanied WU-AX solubilization. Hence, it was active even if most likely a considerable part of its activity was prevented by xylanase inhibitors. A combination of XAA and XBS at a low dosage yielded a distribution of gluten proteins on the different sieves comparable to that of the control. At a high combined dosage, the gluten agglomeration was better than that with XAA alone, indicating that both WE-AX and WU-AX have a negative impact on gluten agglomeration. Finally, experiments with endoxylanase addition at different moments during the separation process suggest that the status of the arabinoxylan population during dough mixing is far less critical for its impact on gluten agglomeration than that during the batter phase.