Urinary phenyl acetate: a diagnostic test for depression?

The compound 2-phenylethylamine is an "endogenous amphetamine" which may modulate central adrenergic functions. 2-Phenylethylamine is mainly metabolized by monoamine oxidase to form phenyl acetate (PAA). The 24-hour urinary excretion of PAA was measured in normal healthy volunteers and depressed patients. Patients were diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders, edition 3. In 70 percent of healthy volunteers of both sexes, the excretion of PAA ranged between 70 and 175 milligrams per 24 hours (mean = 141.1 +/- 10.2). Inpatients with major depressive disorder (unipolar type) (N = 31) excreted less PAA (68.7 +/- 7.0 milligrams per 24 hours) and 55 percent of them excreted less than 70 milligrams per 24 hours; there were no significant differences in the PAA excretion between untreated patients (N = 13) and those treated with antidepressants that were not effective (N = 18). The PAA excretion was reduced to a lesser extent in 35 less severely depressed unipolar outpatients (drug-free for 1 week) (86.3 +/- 11.8 milligrams per 24 hours). These results suggest that low PAA urinary excretion may be a reliable state marker for the diagnosis of some forms of unipolar major depressive disorders.     

Interactive effects of salinity and iron deficiency on different rice genotypes

Salinity adversely affects plant growth, photosynthesis, and availability of nutrients including iron. Rice (Oryza sativa L.) is susceptible to soil salinity and highly prone to iron (Fe) deficiency due to lower release of Fe‐chelating compounds under saline conditions. In order to investigate the effects of salinity and low iron supply on growth, photosynthesis, and ionic composition of five rice genotypes (KS‐282, Basmati Pak, Shaheen Basmati, KSK‐434 and 99417), a solution culture experiment was conducted with four treatments (control, 50 mM NaCl, Fe‐deficient, and 50 mM NaCl + Fe‐deficient). Salinity and Fe deficiency reduced shoot and root growth, photosynthetic and transpiration rates, chlorophyll concentration, and stomatal conductance. The reduction in all these parameters was more in the interactive treatment of salinity and low Fe supply. Moreover, a significant increase in shoot and root Na⁺ with corresponding decrease in K⁺ and Fe concentrations was also observed in the combined salinity and Fe‐deficiency treatment. Among the tested genotypes, Basmati Pak was the most sensitive genotype both under salt stress and Fe deficiency. The genotype KS‐282 performed better than other genotypes under salinity stress alone, whereas Shaheen Basmati was the best genotype under Fe deficiency in terms of all the studied parameters.     

Potassium isotherm partitioning based on modified quantity‐intensity relation and potassium buffering characterization of soils of North India

Background : Potassium (K) availability in soil and plant uptake is restrained by the dynamic interactions among the different pools of K. Aims : To understand these interactions, a study was undertaken to assess the quantity–intensity (Q/I) and buffering characteristics of rainfed maize (Zea mays L.) growing soils. Ten contrasting soils were evaluated for K partitioning changes in exchangeable K (ΔEK) and non‐exchangeable K (ΔNEK) pools in the soil‐solution phase and buffering characteristics using a modified version of Q/I approach. Results : The partitioned Q/I isotherms showed strong adsorption with the increase in K concentration ratio (CR^K) and the changes due to ΔEK were higher than changes due to ΔNEK. Total buffering capacity (PBC^K) significantly correlated (r = 0.92, p <0.01) with clay content with a major share contributed by buffering capacity owing to non‐exchangeable K ( ${\mathrm{PBC}}_{\Delta \mathrm{NEK}}^K$) rather than exchangeable K ( ${\mathrm{PBC}}_{\Delta \mathrm{EK}}^K$). The fixation capacity (β) factor, the magnitude of added K converted into a non‐exchangeable pool, ranged from 41 to 63%, whereas release (α) factor, the magnitude of added K converted to the exchangeable pool, ranged from 19 to 36%. Both threshold solution K (CK_r) and threshold exchangeable K (EK_r) values were found to be high in Satran clay loam (S2) and lower in Doon silty clay loam (S3) soils. The equilibrium exchangeable K (EK_o) was found close to minimum exchangeable K (E_min) in Doon silty clay loam (S3) and Babaweyl sandy clay loam (S1) soils and overall E_min constituted about 8.94 to 0.57% of the EK_o. Conclusion : It may be concluded that K Q/I isotherm partitioning provides a valuable insight to assess the dynamic relations. The ratio of α/β (K recharge index) could be used to evaluate the K enrichment capacity of soil to K additions while EK_r and E_min can be potentially useful in the elucidation of exchangeable K as K fertility index especially in soils with poor K fertilizer management.     

Ruminal dynamics of ad libitum feeding in buffalo bulls receiving different level of rumen degradable protein

Four ruminally cannulated Nili-Ravi buffalo bulls were used in a 4 × 4 Latin Square Design to determine the influence of varying level of ruminally degradable protein (RDP) on dry matter intake (DMI), ruminal characteristics, digestibility, blood pH, blood urea nitrogen (BUN) and nitrogen (N) balance. Four isonitrogenous and isocaloric diets were formulated. The C diet contained 50% RDP while medium, high and very high RDP diets had 66, 82 and 100% RDP of the total crude protein (CP) and were denoted as MRDP, HRDP and VHRDP, respectively. The bulls were fed ad libitum. Nutrients intake decreased linearly with increasing the RDP proportion of total dietary CP. A quadratic effect of RDP on ruminal pH was noticed with increasing level of RDP with quadratic maxima at 66% RDP diet. Increasing level of dietary RDP also had a quadratic effect on total bacterial and protozoal count with maximum microbial count at 82% RDP diet. Increasing dietary RDP resulted in linear increase in DM digestibility. However, neutral detergent fiber digestibility was decreased linearly with increasing the level of dietary RDP. A linear increase in ruminal NH₃–N and BUN was noticed due to increasing level of dietary RDP. Higher positive N balance was noticed in bulls fed C diet compared to those fed MRDP, HRDP and VHRDP diets. The findings of this study indicated that buffalo bulls can effectively utilize 13.12% RDP of DM without any adverse effect on rumen and blood parameters.     

Studies on very-low volume (VLV) water-based sprays for the control of cotton pests

We evaluated, water-based very-low volume (VLV) sprays of various pyrethroids at different dosage levels, comparing these with ultra-low volume (ULV) sprays for the control of cotton pests in the Nampula province of Mozambique. The results showed that karate (lambda-cyhalothrin) 2.5% SG at a dosage level of 15 g a.i./ha applied as VLV gave a significantly higher yield than karate 0.8% ULV (28 g a.i./ha). Also, karate 3.75% WG (12 and 15 g a.i./ha) gave a similar yield to that of karate 0.8% ULV (28 g a.i./ha). Karate 5% EC (15 and 20 g a.i./ha) gave a yield similar to Karate 0.8% ULV (28 g a.i./ha). Baythroid (cyfluthrin) 5% EC at 15, 22.5 and 30 g a.i./ha gave a yield similar to baythroid 1.2% ULV (36 g a.i./ha). Bulldock (beta-cyfluthrin) 12.5% SC at dosage levels of 6, 9 and 12 g a.i./ha gave a similar yield to that using baythroid 1.2% ULV (36 g a.i./ha). In all four trials, water-based VLV sprays gave a yield of cotton and degree of pest-control similar to those obtained with the ULV formulations at comparatively lower dosage levels. Integrated with other pest management practices, these could play an important role in the cost effective management of insect pests of cotton on small-scale farms in many countries.     

Relationship between rhizosphere acidification and phytoremediation in two acacia species

Purpose

Phytoremediation is the most sustainable and economical strategy for reclamation of the salt-affected soils. In order to investigate the relationship between phytoremediation and rhizosphere acidification, two experiments (greenhouse and field) were conducted using two acacia species viz. Acacia ampliceps and Acacia nilotica.

Materials and methods

In greenhouse experiment, both the species were exposed to 100 and 200 mM NaCl concentrations in solution culture. The release of organic acids from plant roots was determined after 14 and 28 days of the salt treatment. Shoot and root ash alkalinity was determined after harvesting the plants. In field experiment, both the species were grown on a saline sodic soil for 2 years. After every 6 months, plant growth data were recorded and soil samples were collected from different soil depths for physicochemical analyses.

Results and discussion

The results of greenhouse study indicated higher rhizosphere acidification by A. ampliceps than A. nilotica in terms of release of citric acid, malic acid, and tartaric acid along with ash alkalinity. The comparison of both the species in the field indicated higher amelioration in the soil properties like pH_s, EC_e, SAR, bulk density, and infiltration rate by A. ampliceps than A. nilotica.

Conclusions

It is concluded from these studies that A. ampliceps is more suitable species than A. nilotica for the phytoremediation of the salt-affected soils due to its higher rhizosphere acidification potential.

     

Influence of mycorrhizal inoculation on carrot growth,metabolites and nutrition

Mycorrhizae are fungal symbionts forming mutualistic relationships with plant roots. This study was undertaken to evaluate the overall influence of arbuscular mycorrhizal fungi (AMF) on the overall growth and development of carrot plant. Surface-sterilized seeds of carrot were sown in earthen pots filled with sterile soil. Half of the pots were inoculated with AMF spores; the other half without any AMF inoculation represented control. After germination inoculated plants, along with the controls, were sampled at 20, 40, 60 and 80 days of growth after seedling emergence. There is a progressive net increase in growth with each 20-day interval after seedling emergence. Storage roots of carrot had a higher level of metabolites and nutrients like nitrogen, phosphorus and potassium in mycorrhiza colonized plants than the control. High performance liquid chromatography analysis indicated an increase in the carbohydrate fractions in AMF-inoculated roots. Thus, mycorrhiza has been found increasing the growth, metabolites and nutrition of carrot plant.     

Genotypic variation in rice for grain yield and quality as affected by salt-affected field conditions

A field experiment was conducted under two natural field conditions at the Research Farm (normal soil) and Proka Farm (salt-affected soil) of The Institute of Soil and Environmental Sciences (ISES), University of Agriculture, Faisalabad, Pakistan, to evaluate the performance of 11 rice genotypes in normal and salt-affected conditions. The experiment was laid out in randomized complete block design (RCBD) with three replications. The gas exchange attributes were measured at vegetative stage whereas the grain and straw yields and the yield components were recorded at maturity. After harvesting, the ionic parameters including sodium (Na⁺) and potassium (K⁺) were determined. Afterward, grain quality in terms of length, width, milling recovery, broken fraction, and chalkiness was also determined for the selected genotypes. Salt-affected conditions adversely affected the physiology, yield, and quality of the tested genotypes. The genotypes KS-282 followed by Shaheen Basmati showed significantly higher photosynthetic rate, transpiration rate, and stomatal conductance under both normal and salt-affected conditions, whereas the genotypes 99404 followed by 99417 showed minimum values of gas exchange attributes. The grain and straw yields were the highest in the case of KS-282 at both sites, whereas the lowest grain and straw yields were observed in the case of 99440 followed by 99417 under both normal and salt-affected conditions. Regarding the quality attributes, Super Basmati produced longer grains but with higher broken fraction and lower milling recovery, whereas the reverse was observed in the case of KS-282.     

Enhanced <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">tuberosi</Emphasis> Resistance in Transgenic Potato Expressing a Rice GLP Superoxide Dismutase Gene

Germin like proteins (GLPs) are a large group of related and ubiquitous plant proteins which are considered to be involved in different processes important for plant development and defense. Multiple functional copies of this gene family have been reported in a number of species (wheat, barley, rice, soybean mosses and liverwort), and their role is being evaluated by gene regulation studies and transgenic approaches. To analyze the role of a rice (Oryza sativa) root expressed germin like protein1 OsRGLP1, for its antifungal activity, transgenic potato plants were developed. These transgenic potato plants were molecularly characterized and biologically assessed after inoculation with Fusarium oxysporum f. sp. tuberosi. Functional analysis showed high accumulation of H₂O₂, increased Superoxide Dismutase (SOD) activity and no oxalate oxidase activity (OxO) in transgenics in comparison to nontransformed control. This increased SOD activity, resistance to heat and sensitivity to H₂O₂ suggest it is a Fe-like SOD. OsRGLP1 expression in potato plants exhibited enhanced resistance in comparison to nontransformed wild type plants suggesting its role in providing protection against Fusarium oxysporum f. sp. tuberosi through elevated SOD level. Overall, results suggest that OsRGLP1 is a candidate for the engineering of potato plants with increased fungal tolerance however, the greater height and tuber number was observed. This phenotype associated with the resistance needs to be evaluated to determine if this is a positive or negative feature.     

Pot study on wheat growth in saline and waterlogged compacted soil: II. Root growth and leaf ionic relations

Soil compaction affects plant growth by causing increased resistance to root penetration and a decreased uptake of water and ions. A pot experiment was conducted to study the effect of soil compaction in conjunction with the soil salinity and waterlogging on root growth and leaf ionic composition of two wheat genotypes (Aqaab and MH-97). Compaction was achieved at a 10% soil moisture content by dropping 5 kg weight, controlled by a tripod stand for 20 times from 0.6 m height on a wooden block placed inside the soil-filled pots. Soil bulk density of non-compact and compact treatments was measured as 1.21 and 1.65 Mg m⁻³, respectively. The desired salinity level (15 dS m⁻¹) was developed by mixing required amount of NaCl in the soil before filling the pots. Waterlogging was developed by flooding the pots for 21 days both at tillering and booting stages. Compaction significantly reduced the root length density (RLD) of both the wheat genotypes while the combined effect of compaction×salinity was more drastic on root length density than compaction alone. Waterlogging however, did not decrease the root length density, rather it mitigated the effect of compaction. Compaction decreased the concentration of K⁺ and K⁺:Na⁺ ratio in leaves. Salinity also decreased the concentration of K⁺ and K⁺:Na⁺ ratio, but increased the concentrations of Na⁺ and Cl⁻ in the crop leaves. Salinity and compaction interacted to cause a greater reduction in K⁺ concentration and the K⁺:Na⁺ ratio, while there was lesser increase in the concentrations of Na⁺ and Cl⁻ compared with salinity alone. Waterlogging also decreased the concentration of K⁺ and K⁺:Na⁺ ratio in leaves. It intensified the effect of salinity but decreased the effect of compaction on leaf ionic composition. Therefore, the effect of compaction on root growth and ion uptake is more severe under salt-affected soil conditions than under normal soil conditions while occasional waterlogging of a compact soil for a few days makes the soil conditions favorable for root growth both under non-saline as well as saline soil conditions. Also, the performance of a genotype in stressed environment is related to maintenance of higher root length density, leaf K⁺ concentration and K⁺:Na⁺ ratio and lower leaf Na⁺ and Cl⁻ concentrations.