全文获取类型
收费全文 | 105篇 |
免费 | 29篇 |
国内免费 | 1篇 |
专业分类
林业 | 3篇 |
农学 | 1篇 |
5篇 | |
综合类 | 18篇 |
水产渔业 | 1篇 |
畜牧兽医 | 101篇 |
植物保护 | 6篇 |
出版年
2023年 | 5篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 8篇 |
2017年 | 6篇 |
2016年 | 13篇 |
2015年 | 8篇 |
2014年 | 5篇 |
2013年 | 9篇 |
2012年 | 1篇 |
2011年 | 2篇 |
2010年 | 3篇 |
2009年 | 7篇 |
2008年 | 3篇 |
2007年 | 5篇 |
2006年 | 6篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 5篇 |
2001年 | 1篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1993年 | 1篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
排序方式: 共有135条查询结果,搜索用时 15 毫秒
21.
Reduction in the mRNA expression of sVEGFR1 and sVEGFR2 is associated with the selection of dominant follicle in cows 下载免费PDF全文
PV Ortega Serrano A Guzmán CG Hernández–Coronado H Castillo‐Juárez AM Rosales‐Torres 《Reproduction in domestic animals》2016,51(6):985-991
The vascular endothelial growth factor (VEGF) is essential for follicular development by promoting follicular angiogenesis, as well as for the proliferation and survival of granulosa cells. The biological effects of VEGF are regulated by two membrane receptors, VEGFR1 and VEGFR2, and two soluble receptors, sVEGFR1 and sVEGFR2, which play an antagonistic role. Thus, the objective of this study was to identify the mRNA expression pattern of total VEGF, VEGFR1, VEGFR2, sVEGFR1 and sVEGFR2 in bovine preselected follicles (PRF) and post‐selected follicles (POF). The mRNA expression of these five genes in both granulosa cells (GC) and theca cells (TC) was compared between follicles classified as PRF and POF based on their diameter and on their ratio of estradiol/progesterone (E2/P4). Results showed a lower expression of mRNA of sVEGFR1 and sVEGFR2 in POF than in PRF (p < .05). Regarding the mRNA expression of total VEGF, VEGFR1 and VEGFR2, there was no difference between POF and PRF follicles (p > .05). Our results showed that the mRNA expression of VEGFR2 and sVEGFR1 was more abundant than the expression of VEGFR1 and sVEGFR2, while GC was the main source of mRNA for total VEGF. On the other hand, TC was the follicular compartment where the receptors were most expressed. Our results suggest that non‐dominant follicles maintain a greater concentration of the mRNA expression of both membrane and soluble VEGF receptors. On the other hand, follicular dominance is related to a reduction in the mRNA expression of sVEGFR1 and sVEGFR2, which may favour VEGF binding with VEGFR2 and, hence, improve the follicular health and development. 相似文献
22.
RM Jerram CG Warman ESS Davies MC Robson AM Walker 《New Zealand veterinary journal》2013,61(4):197-201
CASE HISTORY A 3-year-old male Labrador retriever was presented with a history of dietary indiscretion followed by vomiting and abdominal pain. CLINICAL FINDINGS AND TREATMENT: Abdominal ultrasonography revealed the presence of a fluid-filled cystic structure in the region of the pancreas. Flocculent, dark fluid was drained from the cystic structure during exploratory celiotomy prior to resection of the lateral cystic wall. Omentum was sutured into the cystic cavity to provide drainage and enhance immune response. A jejunostomy feeding tube was placed. Post-surgical care consisted of antibiotic therapy. The dog was clinically normal at 1 and 7 months post-operatively and the pancreas appeared normal on final ultrasonographic examination. CLINICAL RELEVANCE: This case describes the use of omentum to provide physiological drainage of a pancreatic pseudocyst. This surgical technique may help clinicians manage patients with this condition in the future. 相似文献
23.
Effect of the route of administration on the mucosal and systemic immune responses to Lawsonia intracellularis vaccine in pigs 下载免费PDF全文
In an on‐farm study, 40 weaned piglets aged 3 weeks were vaccinated with Lawsonia intracellularis vaccine orally, IM or IP while a fourth group remained unvaccinated. All vaccinated animals showed increased serum levels of L. intracellularis‐specific IgG antibodies, but significantly elevated concentrations of specific IgG, IgA and cytokines were generated in ileal mucosal secretions from the orally and IP vaccinated pigs when examined at 17 days after vaccination. 相似文献
24.
A 4-month old, 200 kg, grey warmblood colt presented for a firm, non painful mass on the distal medial aspect of the left third metatarsus. Excisional biopsy revealed a diagnosis of haemangiosarcoma. Equine haemangiosarcoma is uncommon and only limited reports of successful treatment are available. The prognosis for survival is therefore considered to be poor. After two separate incidences of recurrence with incomplete excision of the tumour, intralesional treatment with cisplatin without excision or debulking was performed on three separate occasions. Intralesional cisplatin injection was performed at monthly intervals for three treatments. Four years post treatment with cisplatin, the horse remained in remission. This case report describes the diagnostic and treatment challenges for successful treatment of a primary haemangiosarcoma on the distal limb of a warmblood foal using intralesional cisplatin chemotherapy. 相似文献
25.
SE RIDGE JT HARKIN† ‡ RT BADMAN† AM MELLOR§ JWA LARSEN¶ 《Australian veterinary journal》1995,72(4):150-153
SUMMARY: Johne's disease was diagnosed in 10 alpacas ( Lama pacos ) in Australia between February 1993 and May 1994. Eight of the animals were between 12 and 24 months of age, one was a 6-year-old female, and one was a 4-year-old male. Five, including the 6-year-old and the 4-year-old alpacas, showed weight loss and diarrhoea before death or slaughter. The other cases showed no clinical signs of Johne's disease but 4 gave a positive result on faecal culture and one gave a positive result on testing with the caprine ACID assay and had acid-fast organisms in its faeces. At necropsy, all cases had grossly enlarged mesenteric lymph nodes. Johne's disease was diagnosed after histological examination of the lymph nodes with conventional culture and polymerase chain reaction testing of tissue samples. This report outlines the clinical, epidemiological, and pathological findings in these cases. 相似文献
26.
Anthony R Palmer Igshaan Samuels Clement Cupido Andiswa Finca W Fransiska Kangombe Isa AM Yunusa 《African Journal of Range and Forage Science》2016,33(1):43-51
Following a field campaign to determine the species composition, canopy cover, aboveground annual production and leaf area index (LAI) of the semi-arid savanna of north-western Namibia, we present a production model that can be used by graziers to determine the livestock carrying capacity. The model predicts the annual aboveground net primary production (ANPP) from regression equations of canopy cover by annual production fraction for plant functional classes. We tested the output of the model against another fully independent net primary production (NPP) model, namely the MODIS NPP product. The mean MODIS NPP for the 29 sites was 343 ± 22?kg dry matter (DM) ha?1 y?1 as opposed to 285 ± 142?kg DM ha?1 y?1 for the fAP model that used the regression method (p < 0.01). As a proof of concept, these landscape-scale ANPP values are used to calculate a recommended livestock carrying capacity for the Ehirovipuka Communal Conservancy, a 1 980 km2 communal area with both wildlife and livestock populations. In addition, we also provide details of a field method for predicting landscape-scale LAI from line transect data. This approach can be used to ground reference the LAI values generated from the MODIS LAI product. 相似文献
27.
Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species 下载免费PDF全文
AM Duszewska P Gręda M Baraniewicz W Bielecki W Niżański A Partyka M Tracz Z Nowak A Chełmońska‐Soyta W Olech 《Reproduction in domestic animals》2018,53(3):818-821
Wisent, or European bison (Bison bonasus), is listed as “vulnerable” on the IUCN Red List of Threatened Species and is therefore protected by international law. For the first time, a Wisent embryo has been obtained in vitro. This procedure creates a new opportunity to protect and increase Wisent reproductive potential and thereby opens new possibilities for the establishment of a controlled and broad reserve of the gene pool. 相似文献
28.
Identification of p53 as a sequence-specific DNA-binding protein 总被引:115,自引:0,他引:115
S E Kern K W Kinzler A Bruskin D Jarosz P Friedman C Prives B Vogelstein 《Science (New York, N.Y.)》1991,252(5013):1708-1711
The tumor-suppressor gene p53 is altered by missense mutation in numerous human malignancies. However, the biochemical properties of p53 and the effect of mutation on these properties are unclear. A human DNA sequence was identified that binds specifically to wild-type human p53 protein in vitro. As few as 33 base pairs were sufficient to confer specific binding. Certain guanines within this 33-base pair region were critical, as methylation of these guanines or their substitution with thymine-abrogated binding. Human p53 proteins containing either of two missense mutations commonly found in human tumors were unable to bind significantly to this sequence. These data suggest that a function of p53 may be mediated by its ability to bind to specific DNA sequences in the human genome, and that this activity is altered by mutations that occur in human tumors. 相似文献
29.
The time dependence of the orientation of Earth's inner core relative to the mantle was determined using a recently discovered 10-degree tilt in the axis of symmetry of the inner core's seismic-velocity anisotropy. Two methods of analyzing travel-time variations for rays traversing the inner core, on the basis of 29 years of data from the International Seismological Centre (1964-1992), reveal that the inner core appears to rotate about 3 degrees per year faster than the mantle. An anomalous variation in inner-core orientation from 1969 to 1973 coincides in time with a sudden change ("jerk") in the geomagnetic field. 相似文献
30.