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T Jauffrais J Kilcoyne V Séchet C Herrenknecht P Truquet F Hervé JB Bérard C Nulty S Taylor U Tillmann CO Miles P Hess 《Marine drugs》2012,10(6):1360-1382
Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell · mL(-1) at steady state in bioreactors 1 and 2, respectively. The AZA cell quota decreased as the dilution rate increased from 0.15 to 0.3 day(-1), with optimum toxin production at 0.25 day(-1). After optimization, SPE procedures allowed for the recovery of 79 ± 9% of AZAs. The preparative isolation procedure previously developed for shellfish was optimized for algal extracts, such that only four steps were necessary to obtain purified AZA1 and -2. A purification efficiency of more than 70% was achieved, and isolation from 1200 L of culture yielded 9.3 mg of AZA1 and 2.2 mg of AZA2 of >95% purity. This work demonstrated the feasibility of sustainably producing AZA1 and -2 from A. spinosum cultures. 相似文献
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Climate change spurs havoc on social-ecological system. People and places vulnerable to climate change have been the focus of many discussions. However, in the forestry sector, limited studies have been conducted that link human vulnerability to recent initiatives, such as reducing emissions from deforestation and forest degradation or REDD+, especially in highly vulnerable countries. Using case studies from the Philippines, this paper focuses on the vulnerability of two community-based forest management (CBFM) organizations, with and without REDD+ readiness intervention, to show the impacts of climate variability and extremes. Two balance-weighted approaches, the livelihood vulnerability index and the IPCC-framework, were used in the vulnerability assessments. Results revealed high vulnerability of both CBFM organizations, although Malitbog Upland Developers for Sustainable Association (MUDSA) livelihood vulnerability is largely aggravated by its exposure to disaster, climate variability and extremes, accessibility to health facilities and water supply. The long history of CBFM implementation in both organizations have minimal contribution in enhancing the adaptive capacity of members to cope with and adapt to climate change impacts. REDD+ (readiness), does not insure reduced vulnerability to climate change, unless sustainable livelihood is achieved. As the State controls forest resources, the rights of CBFM-organizations to commercially benefit from these resources are limited, a disincentive to the development of new and viable development programs in CBFM areas, such as REDD+. This also constrains the achievement of climate resiliency. 相似文献
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Tropical forests worldwide are undergoing rapid changes due to increasing human populations and varied land use practices. In an effort to protect these forests and their species, the number of protected areas has increased exponentially in recent decades. Clearly protected areas play a crucial role in conservation efforts and this strategy has been found to be successful in several studies. However, protected areas are often embedded within a matrix of other land cover types, including non-protected forests, agricultural fields, or urban areas. It is therefore critical for park management to think beyond the borders of their protected areas and work to maintain ecological integrity of the surrounding matrix and the connectivity between protected areas. This study integrates remote sensing, GIS and field observations to examine the effects of different management strategies on land cover changes. As a case study we examine a tiger reserve in Central India which is surrounded by commercially managed forests, representative of many protected areas throughout India. Findings of this study show that while protected areas play an important role in conservation, alternative approaches including favorable management policies could also be effective to extend conservation over larger areas. 相似文献
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For the past decade, supermarket chains have been positioned as the pre-eminent actor in global and national food systems. Some agri-food scholars argue that their ever-expanding transnational supply chains have established an era of stable production-consumption relations (or Food Regime), while others point to the conflicts they are encountering with governments, social movements and ‘alternative’ consumers. However, remarkably little attention has been paid to their relationship with communities and to community system sustainability. Based on fieldwork conducted in the Goulburn Valley, Australia, we argue that supermarket operations are contributing to community tensions through contestation over valued symbols and narratives about what desirable ‘progress’ looks like. We identified three interrelated points of tension being intensified by supermarket chains: whether progress is encapsulated by being an agricultural production or a modern consumption centre; whether progress should be based on a model of corporate capital or the local small business; and to what extent modern citizens can and should support community shopping instead of convenience shopping. For long-time residents, supermarkets are paradoxical actors appealing to, as well as, challenging the narrative of a community whose economic strength was based on the surrounding natural environment and local people’s endeavours. The concepts of solastalgia and structural nostalgia are relevant, with the former referring to the place-based distress experienced by residents whose local area is changing profoundly and the latter describing a process amplifying that distress. Through exploring the political paradoxes of community solastalgia, we raise new questions about supermarket authority within contemporary Food Regimes. 相似文献
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