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161.
Refojo D Schweizer M Kuehne C Ehrenberg S Thoeringer C Vogl AM Dedic N Schumacher M von Wolff G Avrabos C Touma C Engblom D Schütz G Nave KA Eder M Wotjak CT Sillaber I Holsboer F Wurst W Deussing JM 《Science (New York, N.Y.)》2011,333(6051):1903-1907
The corticotropin-releasing hormone receptor 1 (CRHR1) critically controls behavioral adaptation to stress and is causally linked to emotional disorders. Using neurochemical and genetic tools, we determined that CRHR1 is expressed in forebrain glutamatergic and γ-aminobutyric acid-containing (GABAergic) neurons as well as in midbrain dopaminergic neurons. Via specific CRHR1 deletions in glutamatergic, GABAergic, dopaminergic, and serotonergic cells, we found that the lack of CRHR1 in forebrain glutamatergic circuits reduces anxiety and impairs neurotransmission in the amygdala and hippocampus. Selective deletion of CRHR1 in midbrain dopaminergic neurons increases anxiety-like behavior and reduces dopamine release in the prefrontal cortex. These results define a bidirectional model for the role of CRHR1 in anxiety and suggest that an imbalance between CRHR1-controlled anxiogenic glutamatergic and anxiolytic dopaminergic systems might lead to emotional disorders. 相似文献
162.
Marilyn Holly Carla A. Kirts Yvonne Everett Jan L. Flora David O. Hansen Cornelia Butler Flora John A. Hayward David Haenke Alessandro Bonanno 《Agriculture and Human Values》1988,5(4):57-78
Marilyn Holly is an Associate Professor of Philosophy at the University of Florida. Her special interests include political philosophy, ethics and ecology, Jungian thought, and Native American spirituality in its relation to the environment. She is currently at work on a monograph-length piece on the latter topic. She has taught an undergraduate course in ethics and ecology, and plans to do so again. The following essay emerged from her search for a suitable text for such a course. 相似文献
163.
Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila 总被引:31,自引:0,他引:31
B L Tempel D M Papazian T L Schwarz Y N Jan L Y Jan 《Science (New York, N.Y.)》1987,237(4816):770-775
Potassium currents are crucial for the repolarization of electrically excitable membranes, a role that makes potassium channels a target for physiological modifications that alter synaptic efficacy. The Shaker locus of Drosophila is thought to encode a K+ channel. The sequence of two complementary DNA clones from the Shaker locus is reported here. The sequence predicts an integral membrane protein of 70,200 daltons containing seven potential membrane-spanning sequences. In addition, the predicted protein is homologous to the vertebrate sodium channel in a region previously proposed to be involved in the voltage-dependent activation of the Na+ channel. These results support the hypothesis that Shaker encodes a structural component of a voltage-dependent K+ channel and suggest a conserved mechanism for voltage activation. 相似文献
164.
Armstead I Donnison I Aubry S Harper J Hörtensteiner S James C Mani J Moffet M Ougham H Roberts L Thomas A Weeden N Thomas H King I 《Science (New York, N.Y.)》2007,315(5808):73
A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866. 相似文献
165.
Laursen KH Schjoerring JK Olesen JE Askegaard M Halekoh U Husted S 《Journal of agricultural and food chemistry》2011,59(9):4385-4396
The multielemental composition of organic and conventional winter wheat, spring barley, faba bean, and potato was analyzed with inductively coupled plasma-optical emission spectrometry (ICP-OES) and -mass spectrometry (ICP-MS). The crops were cultivated in two years at three geographically different field locations, each accommodating one conventional and two organic cropping systems. The conventional system produced the highest harvest yields for all crops except the nitrogen-fixing faba bean, whereas the dry matter content of each crop was similar across systems. No systematic differences between organic and conventional crops were found in the content of essential plant nutrients when statistically analyzed individually. However, chemometric analysis of multielemental fingerprints comprising up to 14 elements allowed discrimination. The discrimination power was further enhanced by analysis of up to 25 elements derived from semiquantitative ICP-MS. It is concluded that multielemental fingerprinting with semiquantitative ICP-MS and chemometrics has the potential to enable authentication of organic crops. 相似文献
166.
Spatial α-diversity patterns of diverse insect taxa in Northern China: Lessons for biodiversity conservation 总被引:1,自引:0,他引:1
Jan C. Axmacher Yunhui Liu Changliu Wang Liangtao Li Zhenrong Yu 《Biological conservation》2011,(9):2362-2368
Current approaches in terrestrial biodiversity conservation focus predominantly on plants and vertebrates. While these groups account for less than 4% of the estimated global species richness, it is commonly argued that especially the species richness in higher plants is a suitable indicator of overall biodiversity. We tested this assumption, investigating species richness and equitability patterns in three highly species-rich insect families and their links with the vegetation and other environmental factors. Vegetation surveys were combined with pitfall and light trapping to establish the α-diversity of ground beetles, geometrid moths and arctiid moths on 48 plots at varying altitudes between Beijing and the Inner Mongolian Plateau. Soil pH and nutrient status were also recorded. The α-diversity patterns in the three insect families were non-congruent, and links with phytodiversity were weak. The spatial α-diversity patterns in each of the three insect families were significantly correlated with the species density of individual plant families. These links varied between the three insect taxa and were mostly negative in moths. Furthermore, geometrid moth diversity decreased with increasing elevation and decreasing soil pH. Strongly diverging α-diversity patterns across different insect taxa illustrate that it is impossible to find a simple surrogate representing cross-taxon diversity for these highly diverse groups. Furthermore, phytodiversity and vegetation composition appear to play a limited role in governing insect diversity patterns. These results underline the significant risk that current plant-focused approaches in terrestrial biodiversity conservation are inadequate in addressing the conservation needs of the vast majority of species on earth. 相似文献
167.
In laboratory tests, the toxicity of acaricides targeted against house dust mites was tested on five species of stored product
mites (Acarus siro, Aleuroglyphus ovatus, Carpoglyphus lactis, Lepidoglyphus destructor, and Tyroborus lini). The formulations of benzyl-benzoate, benzyl-benzoate/permethrin/pyriproxyfen, and neem were diluted in water and applied
to filter paper in an unventilated chamber. The mortality of mites was observed after 24 h of exposure to acaricide-impregnated
filter paper. All of the tested acaricides were toxic to all of the mite species. There were significant differences in mortality
among the species and the acaricides. Benzyl-benzoate/permethrin/pyriproxyfen was the most effective, followed by benzyl-benzoate
and neem. L. destructor (LD50 0.01–0.11 μg) was the most sensitive mite species, followed by A. siro (LD50 0.04–0.12 μg), T. lini (LD50 2–21 μg), A. ovatus (LD50 3–18 μg), and C. lactis (LD50 4–64 μg). Based on the highly toxic effects of the tested acaricides against the stored product mites, the acaricides should
be considered as a potential tool in the control of stored product mites, although next screening is necessary. 相似文献
168.
Eva VOSLAROVA Petr CHLOUPEK Jan CHLOUPEK Iveta BEDANOVA Vladimira PISTEKOVA Vladimir VECEREK 《Animal Science Journal》2011,82(4):601-606
Effects of different noise levels (70 or 80 dB) that broilers were exposed to during the entire fattening period and also the effect of the timing of the first exposure to intermittent noise in the course of fattening (day 1 vs. day 7) were monitored. After 7 days of exposure to intermittent noise, experimental chickens already showed a significant decrease in live body weight in comparison with the control group. The difference between the group exposed to intermittent noise at 70 dB and the group exposed to 80 dB levels was not statistically significant, although the mean live body weight of broilers in the latter was lower during the entire fattening period. The chickens exposed to intermittent noise from day 1 of age showed lower mean live weight throughout the fattening period compared to chickens exposed to the same level of intermittent noise only from day 7 of age, although at the end of fattening the difference was statistically significant only in chickens exposed to the higher level of intermittent noise (80 dB). 相似文献
169.
Bettschart-Wolfensberger R Dicht S Vullo C Frotzler A Kuemmerle JM Ringer SK 《Veterinary anaesthesia and analgesia》2011,38(3):186-194
ObjectiveTo test if the addition of butorphanol by constant rate infusion (CRI) to medetomidine–isoflurane anaesthesia reduced isoflurane requirements, and influenced cardiopulmonary function and/or recovery characteristics.Study designProspective blinded randomised clinical trial.Animals61 horses undergoing elective surgery.MethodsHorses were sedated with intravenous (IV) medetomidine (7 μg kg?1); anaesthesia was induced with IV ketamine (2.2 mg kg?1) and diazepam (0.02 mg kg?1) and maintained with isoflurane and a CRI of medetomidine (3.5 μg kg?1 hour?1). Group MB (n = 31) received butorphanol CRI (25 μg kg?1 IV bolus then 25 μg kg?1 hour?1); Group M (n = 30) an equal volume of saline. Artificial ventilation maintained end-tidal CO2 in the normal range. Horses received lactated Ringer’s solution 5 mL kg?1 hour?1, dobutamine <1.25 μg kg?1 minute?1 and colloids if required. Inspired and exhaled gases, heart rate and mean arterial blood pressure (MAP) were monitored continuously; pH and arterial blood gases were measured every 30 minutes. Recovery was timed and scored. Data were analyzed using two way repeated measures anova, independent t-tests or Mann–Whitney Rank Sum test (p < 0.05).ResultsThere was no difference between groups with respect to anaesthesia duration, end-tidal isoflurane (MB: mean 1.06 ± SD 0.11, M: 1.05 ± 0.1%), MAP (MB: 88 ± 9, M: 87 ± 7 mmHg), heart rate (MB: 33 ± 6, M: 35 ± 8 beats minute?1), pH, PaO2 (MB: 19.2 ± 6.6, M: 18.2 ± 6.6 kPa) or PaCO2. Recovery times and quality did not differ between groups, but the time to extubation was significantly longer in group MB (26.9 ± 10.9 minutes) than in group M (20.4 ± 9.4 minutes).Conclusion and clinical relevanceButorphanol CRI at the dose used does not decrease isoflurane requirements in horses anaesthetised with medetomidine–isoflurane and has no influence on cardiopulmonary function or recovery. 相似文献
170.
Stengärde L Holtenius K Emanuelson U Hultgren J Niskanen R Tråvén M 《Veterinary journal (London, England : 1997)》2011,190(1):124-130
Sixty dairy herds were studied to investigate the association between long-term incidence of displaced abomasum and clinical ketosis and body condition score and blood profiles, including parameters estimating energy metabolism and hepatic lipidosis in the periparturient period and early lactation. Blood samples were taken around parturition and in early lactation from cows without apparent clinical symptoms of metabolic disorders. A difference in metabolism between high and low incidence herds was shown post-partum by a lower metabolic index (the revised Quantitative Insulin Sensitivity Check Index, RQUICKI), and tendencies for higher concentrations of glucose, insulin and non-esterified fatty acids in the high incidence herds. High incidence herds had more cows and produced on average 1400kg energy-corrected milk per cow per year more than the low incidence herds. No differences were found in parameters reflecting liver cell damage. In the first 3weeks post-partum the RQUICKI was a more sensitive marker of herds with a high incidence of displaced abomasum and clinical ketosis than any of the individual parameters, but further research is needed before practical applications of the RQUICKI can be foreseen. 相似文献