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41.
黄瓜根际解钾细菌的分离筛选、鉴定及其促生效果 总被引:2,自引:0,他引:2
以黄瓜根际土壤为试材,采用亚历山大硅酸盐细菌培养基从黄瓜根际土壤中分离筛选解钾菌,用原子吸收法测定菌株的解钾能力,选出解钾能力较强的菌株进行菌种鉴定,同时研究其对黄瓜幼苗生长的影响。结果表明:从黄瓜根际土壤中共分离获得9株细菌,其中PK1、PK3和PK7具有较强的解钾能力,液体培养96h后其解钾率分别为30.05%、33.78%和44.01%。依据菌株的形态学特征和生理生化特征鉴定,PK1和PK3为恶臭假单胞菌(Pseudomones putida),PK7为胶质类芽孢杆菌(Paenibacillus mucilaginosus)。盆栽试验结果表明,与对照相比,14d后PK1提高黄瓜的根长、株高、鲜质量和干质量分别为42.60%、10.45%、48.65%、33.01%,PK3分别对应提高65.26%、21.09%、83.78%和49.48%,PK7分别对应提高79.46%、44.26%、105.41%和113.03%。 相似文献
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R. Vilcacundo D. Barrio C. Carpio A. García-Ruiz J. Rúales B. Hernández-Ledesma W. Carrillo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):294-300
Quinoa protein concentrate (QPC) was extracted and digested under in vitro gastrointestinal conditions. The protein content of QPC was in the range between 52.40 and 65.01% depending on the assay used. Quinoa proteins were almost completely hydrolyzed by pepsin at pH of 1.2, 2.0, and 3.2. At high pH, only partial hydrolysis was observed. During the duodenal phase, no intact proteins were visible, indicating their susceptibility to the in vitro simulated digestive conditions. Zebrafish larvae model was used to evaluate the in vivo ability of gastrointestinal digests to inhibit lipid peroxidation. Gastric digestion at pH 1.2 showed the highest lipid peroxidation inhibition percentage (75.15%). The lipid peroxidation activity increased after the duodenal phase. The digest obtained at the end of the digestive process showed an inhibition percentage of 82.10%, comparable to that showed when using BHT as positive control (87.13%). 相似文献
44.
R. B. Bassanezi A. B. C. Czermainski F. F. Laranjeira A. S. Moreira P. J. Ribeiro Júnior E. T. Krainski L. Amorim 《Plant pathology》2019,68(1):85-93
Leprosis is caused by the Citrus leprosis virus cytoplasmic type and is vectored by the mite Brevipalpus yothersi. Miticide applications, which cost $54 million annually, are based on inspection for the presence of mites. The aim of the present study was to characterize the spatial patterns of B. yothersi-infested trees and trees with leprosis symptoms for further improvement in sampling and disease control. The presence of mites and the occurrence of leprosis were assessed over two years in 1160 Valencia trees and 720 Natal trees in a commercial sweet orange grove in Sao Paulo State, Brazil. To assess the natural growth and dispersal of mites and leprosis, mite populations were not controlled during the experimental period. Maps of mite-infested trees and trees with leprosis symptoms were analysed at three different levels of spatial hierarchy using complementary methods, i.e. among adjacent trees within and across rows, within quadrats, and the strength and orientation of aggregation among quadrats. The study showed that the spatial patterns of virus-infected and mite-infested trees were different, with a strong aggregation pattern of trees with leprosis symptoms that increased over time. Conversely, the spatial pattern of B. yothersi showed randomness or weak aggregation at all three spatial hierarchical levels. Disease incidence increased steadily in plots of both cultivars, unlike in mite-infested trees where incidence fluctuated over time. These results have important implications for the development of better management strategies for leprosis. Sampling methods and action thresholds for mite control should consider primary disease inoculum in addition to the incidence of mites. 相似文献
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Spyra Marcin Kleemann Janina Cetin Nuket Ipek Vázquez Navarrete Cesar Jesús Albert Christian Palacios-Agundez Igone Ametzaga-Arregi Ibone La Rosa Daniele Rozas-Vásquez Daniel Adem Esmail Blal Picchi Paolo Geneletti Davide König Hannes J. Koo HongMi Kopperoinen Leena Fürst Christine 《Landscape Ecology》2019,34(7):1715-1735
Landscape Ecology - Several case studies investigated the role of ecosystem services in participatory planning processes. However, no systematic study exists that cuts across a large number of... 相似文献
47.
矩形薄壁侧堰水力特性试验研究 总被引:2,自引:2,他引:0
侧堰作为一种量水设施,精度较高、结构简单、安装和拆卸方便,较易在灌区应用和推广,有很大的研究价值。对不同宽度和高度的矩形侧堰在缓流条件下进行了试验,分析了各工况下矩形渠道侧堰上、下游水面线、能量分布、流量系数等水力因素。结果表明,主渠道侧堰段能量基本保持不变,符合De Marchi的恒定能量假定;主渠道靠近侧堰边壁、中心线以及另一边壁三处的水面波动程度不同,说明侧堰对堰前水流产生影响;基于De Marchi假定和无量纲分析原理得到的流量公式最大相对误差均在±10%以内,满足灌区量水精度要求。 相似文献
48.
高效脱色菌的特性及其在染化废水厌氧处理中的生物强化作用 总被引:14,自引:0,他引:14
从活性污泥等微生物源中分离、筛选获得10株高效脱色菌,经鉴定其属于Pseudomonas,Bacillus,Xanthomonas,Erwinia,Alealigenes,Plesiomonas。对艳红染料生产废水脱色试验表明最适温度30℃,pH6.6~8.0,湿细胞浓度20~30!g*L-1;静置培养(兼氧)脱色率高于振荡培养;外加NADH、NADPH、易降解有机物和有机废水可强化脱色菌的脱色性能。为实现脱色菌的资源化应用,试验将PseudomonasD18,ErwiniaD17,AlcaligenesD19andPlesiomonasD124株脱色菌制成混合培养物和固定化细胞,分别投加于处理染化废水的厌氧反应器,结果发现在相同COD负荷、水力负荷条件下,投加固定化细胞的反应器,其脱色率可提高5!%~10!%,出水苯胺浓度提高40!%~65!%。电镜观察发现脱色菌在胶团内外持留、增殖。厌氧反应器性能的改善主要通过功能性微生物生物量和相关基因库量的增加而实现。 相似文献
49.
Sanchez R Vázquez A Riquelme D Villén J 《Journal of agricultural and food chemistry》2003,51(21):6098-6102
A fully automated on-line reversed phase liquid chromatography-gas chromatography system is described. The system uses a prototype of the automated through oven transfer adsorption desorption interface. The system is demonstrated by presenting a new rapid method for the determination of pesticide residue in olive oil, which is injected directly with no sample pretreatment step other than filtration. Methanol:water is used as the eluent in the LC preseparation step, while the LC fraction containing the pesticide is automatically transferred to the gas chromatograph. Detection limits of pesticides varied from 0.18 to 0.44 mg/L when a flame ionization detector was used. As an example, relative standard deviation and linear calibration are presented for terbutryne. 相似文献
50.
林地开垦后坡面侵蚀过程与土壤养分流失的研究 总被引:14,自引:8,他引:14
林地被人为开垦破坏后 ,加速侵蚀是导致坡面土壤养分流失的根本原因。坡面土壤养分流失的空间分布与坡面侵蚀方式和侵蚀强度的空间分布相一致 ,浅沟沟槽是坡面土壤养分流失最严重的部位。土壤养分流失在开垦初期非常迅速 ,开垦耕种 5 a后 ,土壤养分含量可减少 5 0 %。土壤剖面中 A层的流失是土壤养分流失的标志。 相似文献