Relationship of p21WAF1 gene polymorphisms with protein expression in breast carcinomas

AIM: To investigate the relationship between p21^WAF1gene polymorphisms and protein expression in breast carcinoma. METHODS: Polymerase chain reaction single-strand conformation polymorphisms technique (PCR-SSCP) and immunohistochemical assay of S-P immunostaining technique were used to study polymorphisms of p21^WAF1 and protein expression respectively on the specimen of paraffin-embedded tissues in 100 cases of breast carcinomas and 40 benign breast diseases as control. RESULTS: Two p21^WAF1 gene polymorphisms were found in 18% (18/100) of breast carcinomas and 5% (2/40) of control samples. The difference between the two groups was statistically significant (χ²=3.94, P＜0.05). The positive immunohistochemical reaction of p21^WAF1 protein were found in 50% (50/100) of breast carcinomas and 12.5% (5/40) of control samples. The difference between the two groups was statistically significant (χ²=16.84, P＜0.01). The positive immunohistochemical reaction of p21^WAF1 protein were found in 100% (18/18) of breast carcinomas with p21^WAF1 gene polymorphisms and 39% (32/82) of no p21^WAF1 gene polymorphisms. The difference between two groups was statistically significant (χ²=21.95, P＜0.01). The p21^WAF1 gene polymorphisms were correlated with the protein expression in breast carcinomas (r=0.576, P＜0.01). CONCLUSION: p21^WAF1 gene polymorphisms may create the different copies of mRNA and may make relevant protein molecules.     

Differential regulating effect on estrogen receptor α and β expression in female rat hippocampus and cortex regions between different types of estrogen regents

AIM: To study the regulatory effect two different estrogen reagents on expressions of estrogen receptor α and β in female rat hippocampus and cortex regions. METHODS: 12 cycles after ovariectomy, female rats were orally injected with premarin or progynova for 3 cycles before sacrificed. Semiquantitative RT-PCR was used to detect the ERs mRNA expression and SP immunohistochemistry was performed to measure the ERs protein distribution and expression. RESULTS: In premarin group, ER α mRNA levels in both hippocampus and cortex tissues decreased significantly compared with control. ER α protein level in hippocampus was lower than that in the control. However, ER α protein level in cortex had no statistical difference. ER β mRNA in the two regions and ER β protein in cortex had no statistical differences compared with control, while ER β protein level in hippocampus was higher than that in the control. In progynova group, both mRNA and protein levels of ER β increased significantly in the two regions compared with the control, and ER α mRNA level also increased in hippocampus, but ER α mRNA level in cortex and ER α protein levels in the above two regions showed no statistical differences. CONCLUSION: There were differential regulatory effects on ER α and ER β expression in female rat cognitive regions between the two different types of estrogen reagents, which may be one of the mechanisms of varied effects in different estrogen replacement therapy reagents.     

猪伪狂犬病基因缺失疫苗的制备、安全性、免疫原性、保存期测定及区域试验

为了提供有效的伪狂犬病疫苗，用鸡胚成纤维细胞扩大培养了PrV HB-98突变株（TK^-/gG^-/LacZ^＋），研制了伪狂犬病基因缺失疫苗，并对该疫苗经肌肉接种、经口等免疫途径的最小免疫剂量进行了测定，同时也对4批疫苗的安全性、效力、免疫期和保存期进行了检测；同时将4批疫苗用于免疫23个猪场的母猪、新生仔猪和育肥猪进行区域试验。测定结果表明，疫苗经上述两种途径接种对不同阶段猪的最小免疫剂量均为10^5.0 TCID50；10倍免疫剂量的疫苗对初生仔猪、15日龄仔猪和妊娠母猪是安全的，免疫猪能抵抗强毒的攻击；疫苗在4℃和-20℃下分别可保存6个月和12个月。对伪狂犬病毒抗体阴性的70日龄商品猪和种猪的免疫期为6个月。田间试验表明，4批猪伪狂犬病基因缺失疫苗安全有效，并可用于仔猪发病时的紧急接种。为猪伪狂犬病基因工程疫苗的制备与应用提供了有力的依据。     

猪圆环病毒衣壳蛋白单克隆抗体的制备

以大肠杆菌表达的猪圆环病毒2型(PCV2)重组衣壳蛋白(GST-CAP2)为抗原,以一定的免疫程序接种BALB/c小鼠后,取其脾细胞与骨髓瘤细胞SP2/0按常规杂交瘤技术进行融合.以GST-CAP2以及猪圆环病毒1型(PCV1)重组衣壳蛋白(GST-CAP1)为包被抗原,经间接ELISA筛选获得2株能稳定分泌针对PCV的单克隆抗体的杂交瘤细胞株(M1和G1).通过dot-ELISA、Western-blotting以及间接免疫荧光(IFA)技术证明G1株分泌的单抗同时识别PCV1和PCV2, 而M1株分泌的单抗则特异性针对PCV2.该单抗的制备将为进一步建立快速检测PCV的方法奠定基础.     

脑部特异表达rtTA调控元件转基因小鼠的构建

为获得一种可调控基因表达系统,避免传统转基因在胚胎发育早期表达的毒性作用或致死作用以及基因过度表达而造成难于分析的复杂多表型,应用分子生物学方法将四环素反式激活子rtTA克隆到仅在脑部能被激活的钙调蛋白依赖性蛋白激酶CaM kinase Ⅱα亚基启动子之后,将其通过显微注射方法注入FVB小鼠受精卵雄原核中获得四环素调控系统中反应组分的转基因工具鼠.经PCR及Southern检测,最终得到3只阳性小鼠(1公2母),表达检测结果显示,其中仅2只(1公1母)后代检测到rtTA表达产物.结果表明已成功构建四环素调控系统中的转基因工具鼠,为今后研究其他脑部基因功能提供便利.     

微波干制紫心甘薯片干燥特性及其对色素的影响

进行了微波干燥紫心甘薯片脱水试验,获得了微波干燥紫心甘薯片失水规律,建立了紫心甘薯片微波干燥模型,以采用Page方程拟合较好。考察微波干燥对紫心甘薯色素的影响,结果表明：在微波功率700W、切片厚度6mm、前期干燥时间20～50s,获得的产品色素含量较高。     

小麦条锈病气象预测方法及遥感监测研究进展

从影响小麦条锈病发生发展的大尺度气候背景、局地气象因子两方面,对小麦条锈病发生发展的气象预测研究进行了综述,指出了常规预测方法存在的问题和难点。概述了高光谱遥感在小麦条锈病监测中的应用,并对遥感与作物模型的结合进行了探讨,在此基础上展望了基于大气环流的小麦条锈病中、长期预测及G IS辅助的遥感技术在小麦条锈病监测方面的应用前景。     

"星座"聚类法在农用地分等因素指标区划分中的应用——以陕西省合阳县为例

以陕西省合阳县为研究对象,以定量划分农用地分等因素指标区为目的,首先应用灰色关联理论对影响农用地等别的各个因子进行灰色关联分析,在此基础上采用"星座"聚类分析法对农用地分等因素指标区进行定量划分。运用该种方法对农用地分等因素指标区的划分与当地实际情况相符合,为进行农用地分等工作提供了科学依据。     

6种蔷薇的核形态学研究

蔷薇属植物染色体核型分析是其远缘杂交利用的细胞学基础.采用常规压片法对6个蔷薇原种根尖染色体制片中的预处理、解离、染色方法等进行了比较,并对其核形态学进行了研究.结果表明:根尖用0.002 mol/L 8-羟基喹啉在18~20 ℃下处理4 h、5 N HCl室温下解离25~30 min、改良卡宝品红染色2 h以上效果较好,染色体数目及形态分别为:多花蔷薇 (Rosa multifloraThumb)为2n=2x=14=14m,粉团蔷薇(Rosa multifloravar catheyensis)为2n=3x=21=15m+6sm,金樱子(Rosa laevigataMichx)为2n=2x=14=10m+4sm,日本无刺蔷薇(Rosa multiflora var)为2n=2x=14=10m+4sm, 硕苞蔷薇(Rosa bracteataWendl)为2n=2x=14=14m, 香水月季[Rosa odorata (Andr.) Sweet]为2n=2x=14=12m+2sm.其中,粉团蔷薇的核型类别为2A型,日本无刺蔷薇为2B型,其他品种均为1A型.     

永丰县木本粮食植物资源

介绍永丰县山林中木本粮食植物资源。