Salicylic acid improves the antioxidant ability against arsenic-induced oxidative stress in sunflower (Helianthus annuus) seedling

The present study investigated the potential role of external salicylic acid (SA) in alleviating Arsenic (As) toxicity in sunflower leaves. The exposure of plants to 10 µM As inhibited biomass production and intensively increased the accumulation of As in both roots and leaves. The levels of some important parameters associated with oxidative stress, namely lipid peroxidation, electrolyte leakage, and hydrogen peroxide (H₂O₂) production were increased. SA application alleviated the negative effect of As on growth and led to decrease in oxidative injuries. Furthermore, SA application led to higher activity of catalase, ascorbate peroxidase, and glutathione peroxidase, and concomitantly decreased superoxide dismutase and guaiacol peroxidase activities. As important antioxidants, ascorbate and glutathione contents in sunflower leaves exposed to As were significantly decreased by SA treatment. These results reveal that SA is more effective in alleviating As toxicity at higher concentrations than that at lower concentrations.     

Bovine herpesvirus-1 (BHV-1), bovine leukemia virus (BLV) and bovine viral diarrhea virus (BVDV) infections in Algerian dromedary camels (<Emphasis Type="Italic">Camelus dromaderius</Emphasis>)

This study was performed to investigate the presence of bovine herpesvirus-1 (BHV-1), bovine leukemia virus (BLV) and bovine viral diarrhea virus (BVDV) infections in dromedary camels (Camelus dromaderius) kept in mixed herds with sheep and goats in Algeria, since the prevalence of BHV-1, BVDV, and BLV infections among dromedary camels in Algeria is unknown. Totally, 111 camel sera were collected from two provinces (Laghouat and Ghardaia) in Algeria. The sera were analyzed for BHV-1 specific antibodies, BVDV specific antibodies and BVDV antigen using the ELISA, and BLV nucleic acid using PCR. The seropositivity rate was 9.0% for BVDV-specific antibody, although 41.4% of camels tested were positive for BVDV antigen. Moreover, there was no evidence of BHV-1 and BLV infections. The results indicated that camels might represent an important source for BVDV infection in all ruminants, including cattle, sheep, and goats bred in mixed herds in Algeria, since they had a higher BVDV prevalence rates. Therefore, the prevention and control measures for BVDV infection should put in place in camel populations to limit the spread of BVDV infection to ruminant populations in Algeria.     

Therapeutic trial of fluvastatin in a cell line xenograft model of canine mammary gland cancer

The Hippo signalling pathway is involved in breast cancer and canine mammary tumour (CMT). This study sought to evaluate the efficacy of fluvastatin on the Hippo pathway and its main effectors, YAP and TAZ, in vivo in a murine CMT cell line xenograft model. On treatment day 1, mice were divided into four groups: vehicle, fluvastatin, doxorubicin or a combination therapy. Tumour volumes were monitored with callipers and tissues harvested on day 28th of treatment. Histopathological examination of tumour tissues and major organs was performed as well as tumour evaluation of necrosis, apoptosis, cellular proliferation, expression of YAP, TAZ and the mRNA levels of four of their target genes (CTGF, CYR61, ANKRD1 and RHAMM2). Results showed a statistically significant variation in tumour volumes only for the combination therapy and final tumour weight only for the doxorubicin group compared to control. There was no significant difference in tumour necrosis, expression of CC3, ki-67, YAP and TAZ measured by immunohistochemistry and in the mRNA levels of the target genes. Unexpectedly, lung metastases were found in the control group (9) and not in the fluvastatin treated group (7). In addition, mass spectrometry-based quantification of fluvastatin reveals concentrations comparable to levels reported to exert therapeutic effects. This study shows that fluvastatin tumours concentration reached therapeutic levels without having an effect on the hippo pathway or various tumour parameters. Interestingly, only the control group had lung metastases. This study is the first to explore the repurposing of statins for cancer treatment in veterinary medicine.     

名优茶连续式理条机参数优化设计与试验

针对传统茶叶理条做形装备主要依靠经验法设计,存在做形质量不佳的问题,该研究运用数值法对连续式茶叶理条机结构与作业参数进行优化,以改善连续式理条机的理条作业性能。通过对连续式理条机进行运动学分析,得到锅槽速度、加速度表达式,并探究锅槽运动与茶叶理条状态之间的关系;通过研究茶叶在连续式理条机锅槽内的运动规律和受力情况,建立茶叶-正U形槽动力学模型,分析了茶叶在锅槽运动周期内各阶段的受力特点及对茶叶成形的作用;运用EDEM软件建立茶叶理条仿真模型,对影响茶叶成形的曲柄转速、锅槽振幅、锅槽倾角、凸棱个数和凸棱高度等关键设计参数进行仿真模拟,得到上述因素作用下茶叶颗粒的平均速度、平均受力与做形时间的关系曲线,并以仿真结果为依据设计三因素三水平正交试验,以成条率、碎茶率为评价指标,利用Design-Expert软件进行数据处理与回归分析,并对优化结果进行验证试验。仿真结果表明：曲柄转速、锅槽振幅和凸棱个数等对理条质量影响较大,茶叶纵向占比率随理条时间增加而升高,促使茶叶主茎脉轴线方向受力形成条状外形;以曲柄转速为195 r/min、锅槽振幅为99 mm、凸棱个数为2的最优参数组合进行验证,成条率为87.39%,碎茶率为1.85%,与优化结果基本一致。研究结果可为连续式茶叶理条机优化设计提供理论参考。     

A GIS-based susceptibility indexing method for irrigation and drinking water management planning: Application to Chebba-Mellouleche Aquifer, Tunisia

Locating high quality groundwater resources in semi-arid regions with growing population and agricultural development is an expensive undertaking. Simple susceptibility indexing method, based on vulnerability and quality index, can be used to facilitate this application. The GIS technique provides an efficient environment to reach this objective. The contamination susceptibility index was calculated by taking the product of the vulnerability index (VI) and the quality index (QI). The VI index was calculated using DRASTIC method to evaluate the hydrogeological characteristics of the Aquifer. The quality index calculation procedure, based on the water classification, was introduced to evaluate hydrochemical data. The susceptibility indexing method was applied in the Chebba-Mellouleche Aquifer, located in Eastern Tunisia. The results show a clear degradation of the water quality throughout the Aquifer. In addition, the susceptibility indexing map which incorporates hydrogeological and hydrochemical datasets reveals a similarity with areas of high anthropogenic activities. Thus, it is more realistic to estimate the groundwater contamination. The main aim of this study is to give an overview of the drinking and irrigation water quality in relation to the hydrogeological characteristics of the Chebba-Mellouleche Aquifer. This overview can form the basis for further investigations.     

Assessment of groundwater risk using intrinsic vulnerability and hazard mapping: Application to Souassi aquifer, Tunisian Sahel

Special attention has been paid to risk assessment in water resource management in arid and semi-arid regions like the Souassi aquifer, Tunisia. Risk assessment, using vulnerability and hazard mapping, is considered as a fundamental aspect of sustainable groundwater management. To determine the degree of risk affecting the study area, an attempt has been made to combine hydro-geologic parameters using the DRASTIC method and the hazard assessment by taking the product of the weighted hazard value (H_I), the ranking factor (Q_N) and the reduction factor (R_f).All parameters used in this risk assessment were prepared, classified, weighted and integrated in a GIS environment. Data treatment shows that large areas in the Souassi aquifer can be classified as high or very high risk areas corresponding to pollution sources located in high vulnerability zones. The areas with low and very low risk are situated in the south, which could, consequently, be interesting for future development and long term planning of protective measures. Sensitivity analyses indicated that the removal of groundwater depth, net recharge and aquifer media parameters from the DRASTIC index, causes large variation in vulnerability assessment. Moreover, hydraulic conductivity and topography were found to be more effective in assessing aquifer vulnerability. Therefore, they should have higher weights than those assumed by the DRASTIC standard method, and contrary to the impact of the vadose zone parameter. The validity of the DRASTIC and the risk methods, verified by comparing the distribution of nitrates in the groundwater and the different vulnerability classes, shows a high similarity.