Nutrient content of maize and soil organic matter status under various tillage methods and farmyard manure levels

Abstract

A two-year field study was conducted under semi-arid conditions in Pakistan to assess the role of tillage systems and farmyard manure on soil, plant nutrients and organic matter content. Four tillage systems (zero, minimum, conventional and deep tillage) and three farm manure levels were used. Maize crop was grown up to maturity. Uptake of N, P and K in maize shoots improved in tillage systems compared to non-tillage and treatments where farmyard manure was applied. Soil N status decreased in the deep tillage systems, whereas it increased in all other tillage systems and in the farmyard manure amended treatments. Increase in soil P values was observed under minimum and conventional tillage, whereas deep tillage resulted in a decrease. Farmyard manure amendments increased soil P and soil K in all systems. Tillage did not affect soil K levels. Results demonstrate that reduced tillage is practicable in arid and semi-arid regions since it improves soil fertility.     

Effects of seed priming with plant growth-promoting rhizobacteria on wheat yield and soil properties under contrasting soils

In the present investigation, different strains of Plant growth-promoting rhizobacteria (PGPR), namely Bacillus megaterium, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their growth-promoting effects on wheat as well as on soil properties under field conditions at two different sites having sandy loam and silt loam type of soils. PGPR strains were evaluated either singly or in consortia. Amongst all the treatments, wheat inoculated with consortia was found most effective as it increased grain yield up to 53% over control in silt loam soil, whereas, corresponding effects in sandy loam soil were less pronounced as an increase of 31% was observed in corresponding treatments, respectively. Enhanced effects on soil properties were also more intense in silt loam as there was an increase of 205% organic matter as against sandy loam soil where this value was 110%.     

Dinitrogen fixation and growth responses to phosphorus and aluminum application in pigeon. pea (Cajanus cajan L.)

Abstract

Responses to aluminum phosphate (Al-P) and superphosphate (Sup-P) application in terms of dry matter production by pigeon pea and soybean were compared under field conditions. The effect of aluminum on growth and symbiotic dinitrogen fixation was examined under hydroponic conditions. In a field experiment, Al-P application significantly increased whole plant dry weight and P amount in pigeon pea, whereas in soybean, Al-P application had no significant effect on the two parameters. The results indicate that P absorption ability from Al-P is higher in pigeon pea compared to soybean. With Al-P application, leaf and whole plant Al concentration in pigeon pea significantly increased unlike in soybean.

Under hydroponic conditions, 50 nig L^?1 Al application increased the plant weight of pigeon pea while it decreased that of soybean. This treatment decreased the total nodule activity of soybean more appreciably than that of pigeon pea. Under the Al treatment conditions, whole plant P amount increased in pigeon pea, whereas it decreased in soybean, and the amount of Al increased more appreciably in pigeon pea, most of which was deposited in roots.

These results suggest that compared to soybean, pigeon pea displays a higher ability to absorb P from P compounds with a low solubility i.e. Al-P and that it is more tolerant to Al toxicity.     

A Study on Seasonal Variability of Trace Elemental Status of Forages for Grazing Ruminants

An investigation was conducted to determine the trace- mineral concentrations of forages in relation to requirements of ruminants grazing in natural pastures in the province of Punjab, Pakistan during two different seasons. Animals were closely followed during grazing and forages corresponding to those consumed by the animals were taken and analyzed for copper (Cu), iron (Fe), zinc (Zn), manganese (Mn), and cobalt (Co) concentrations. The data on the trace- mineral concentrations showed that most of these minerals varied greatly as a function of seasons and sampling periods. The forage Cu, Fe, and Zn concentrations were affected by seasonal changes but no influence of season was observed on the concentrations of forage Mn and Co concentration. Forage Zn and Co, during both seasons and at some sampling intervals, and forage Mn during summer were at marginal deficient levels, and in contrast, all other forage trace-minerals were within the required range for ruminants during both seasons. Based on these results, the supplementation of Zn, Co, and Mn would seem most important to support optimum livestock productivity.     

Soybean preference for Bradyrhizobium japonicum for nodulation

For the increase of the occupation ratio of inoculum strain in the competition with indigenous rhizobia, the relationship between Rj-genotypes of soybean and the preference of Rj-cultivars for various types of rhizobia for nodulation was investigated by using the Rj ₂ Rj ₄-genotype of soybean isolated from the cross between the Rj ₂ Rj ₃-cultivar IAC-2 and Rj ₄-one Hill (Ishizuka et al. 1993: Soil Sci. Plant Nutr., 39, 79-86). Firstly, these Rj ₂ Rj ₄-genotypes were found to harbor the Rj ₃-gene. The Rj ₂Rj₃Rj₄-genotypes of soybean were considered to exhibit a more narrow microsymbiont range for nodulation than the Rj ₂ Rj ₃-and Rj₄-cultivars. Therefore, rhizobia were isolated from the nodules of various Rj-genotypes of soybeans grown in soils, and the preference of the Rj ₂ Rj ₃ Rj ₄-genotype for indigenous rhizobia was examined. The nodule occupancy of serotype 110 was significantly higher in the bacteroids of the nodules from the Rj ₂ Rj ₃ Rj ₄-rgenotypes than in those from the other genotypes, non Rj-, Rj ₂ Rj _3-, and Rj ₄-cultivars. These results demonstrated that the Rj ₂ Rj ³ Rj ⁴-genotype prefers more actively serogroup USDA110 to the others of rhizobia. Thus, Rj ₂ Rj ₃ Rj ₄-genotype is superior to non- Rj-, Rj ₂ Rj ₃-, and Rj ₄-genotypes for the formation of efficient nodules for nitrogen fixation.     

Application of the CSM-CERES-Rice model for evaluation of plant density and irrigation management of transplanted rice for an irrigated semiarid environment

The objectives of this study were to evaluate the performance of the Cropping System Model (CSM)-CERES (Crop-Environment Resource Synthesis)-Rice for simulating growth and yield of rice under irrigated conditions for a semiarid environment in Pakistan and to determine the impact of plant density and irrigation regime on grain yield and economic returns. The crop simulation model was evaluated with experimental data collected in 2000 and 2001 in Faisalabad, Punjab, Pakistan. The experiment utilized a randomized complete block design with three replications and included three plant densities (one seedling hill^?1, PD₁; two seedlings hill^?1, PD₂; and three seedlings hill^?1, PD₃) and five irrigation regimes (625 mm, I₁; 775 mm, I₂; 925 mm, I₃; 1075 mm, I₄; and 1225 mm, I₅). To determine the most appropriate combination of plant densities and irrigation regimes, four plant densities from one seedling hill^?1 to four seedlings hill^?1 and 17 irrigation regimes ranging from 0 to 1600 mm, for a total of 68 different scenarios, were simulated for 35 years of historical daily weather data. The evaluation of CSM-CERES-Rice showed that the model was able to accurately simulate growth and yield of rice for irrigated semiarid conditions, with an average error of 11% between simulated and observed grain yield. The results of the biophysical analysis showed that the combination of the two seedlings hill^?1 plant density and the 1,300 mm irrigation regime produced the highest yield compared to all other scenarios. Furthermore, the economic analysis through the Mean-Gini Dominance (MGD) also showed the superiority of this treatment compared to the other treatment combinations. The mean monetary return ranged from ?47 to 1,265 $ ha^?1 among all 68 scenarios. However, to be able to furnish the demand of rice grain for local consumption and to increase export, there is a need to expand this technology among the rice growers of other rice producing areas in Pakistan through extension workers.     

Molecular basis of Iron Biofortification in crop plants; A step towards sustainability

Iron is one of the most important micronutrients for crop plants due to its use in important physiological processes such as photosynthesis, mitochondrial respiration, metal homeostasis, and chlorophyll synthesis. Crop plants have adapted different strategies for uptake, transport, accumulation, and storage of iron in tissues and organs which later can be consumed by humans. Estimates indicate that about 2 billion people (33% of human population) are at risk of iron deficiency in which infants, children, and pregnant women are potentially compromised. Biofortification refers to the increase in concentration of micronutrients in edible parts of plants and understanding the pathways for iron accumulation in plants is necessary for breeding iron‐enriched crops. Iron‐biofortified crops are also one of the key factors in achieving multiple United Nations Sustainable Development goals. This review article covers different strategies of iron acquisition and transport in plants, its bioavailability, coping with the iron deficiency as a global perspective, the current status of iron biofortification, and how breeding future biofortified crops could be helpful in combating the said issue in a sustainable manner.     

Variability in seed traits and genetic divergence in a clonal seed orchard of Dalbergia sissoo Roxb.

The variations in seed and pod traits, genetic superiority and genetic divergence were evaluated for a Clonal Seed Orchard (CSO) of Dalbergia sissoo Roxb. at Bithmera, India consisting of 20 clones from different agro-climatic conditions of four northern states (Uttar Pradesh, Uttarakhand, Haryana and Rajasthan). The seeds and pods of various clones in the orchard exhibited significant variability in size, weight and other characters. Significant positive correlations were observed between seed length and seed width (p<0.05), seed length and seed thickness (p<0.01), seed length and seed weight (p<0.01), seed thickness and seed weight (p<0.01), seed length and germination value (p<0.05). The genetic parameters for seed and pod traits also showed a wide range of variations in the orchard. Heritability values were found to be over 50 percent for most of the seed and pod traits. Seed weight, seed length and seed thickness showed high heritability values coupled with maximum genetic gain for these characters. Ward’s minimum variance dendrogram of clones of D. sissoo showed three distinct clusters; cluster 1 was the largest with 12 better clones whereas cluster 2 and 3 consisting of seven moderate clones and one poor clone, respectively. Mean cluster values showed sufficient variation among the clusters for seed weight, germination value and seed length. The possible hybridization between best clones of cluster 1 to the disease resistant clone of cluster 2 (resistant against deadly Gandoderma lucidum root rot disease of D. sissoo) is also suggested for further breeding programmes of the species. The deployment of clone 194 (better performed and disease resistant) is also recommended in future plantation programmes of D. sissoo in northern India.     

Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens

This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (p<0.05) lymphoproliferative responses in chickens administered with ethanolic extract of A. vera as compared to those administered with aqueous extract and control group. Microplate haemagglutination assay for humoral response on day 7th and 14th post primary and secondary injections of sheep red blood cells (SRBCs) revealed significantly higher (p<0.05) anti SRBC antibody (total Igs, IgG and IgM) titers in chickens of experimental groups as compared to the control group. None of the extracts, however, demonstrated significant effects on the development of lymphoid organs. Results of experiment-II revealed maximum protection (60%) in chickens administered with aqueous Aloe extract as compared to the ethanolic extract administered chickens (45%). Mean oocysts per gram of droppings in the control group was significantly higher (p<0.05) as compared to the chickens in both the experimental groups. Chickens administered with aqueous Aloe extract showed a minimal mean lesion score (2.3) followed by those administered with ethanolic Aloe extract (2.6) and control chickens (3.05) for caeca, and a similar pattern was observed for intestinal lesion scoring. Further, significantly higher weight gains and antibody titers (p<0.05) were observed in chickens administered with A. vera extracts as compared to those in the control group. It was concluded that A. vera may be a potential and valuable candidate to stimulate the immune responses and can be used successfully as an immunotherapeutic agent against coccidiosis in industrial broiler chickens.