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261.
Saridomichelakis MN Athanasiou LV Salame M Chatzis MK Katsoudas V Pappas IS 《Veterinary dermatology》2011,22(5):429-435
The aim of this cross-over study was to compare clindamycin pharmacokinetics in the serum of clinically normal dogs when administered orally at two dosage regimens (5.5 mg/kg, twice daily, and 11 mg/kg, once daily), separated by a 1 week wash-out period. Serum samples were obtained from six clinically normal laboratory beagles before, 3, 6, 9 and 12 h after the first and fifth dose of clindamycin at 5.5 mg/kg, twice daily, and before, 3, 6, 9, 12, 18 and 24 h after the first and third dose at 11 mg/kg, once daily. Serum clindamycin concentrations were determined by reverse-phase liquid chromatography coupled with mass spectrometry. Results were analysed using Student's paired t-test, at a 5% level of significance. Values of pharmacokinetic parameters that differed significantly between the two dosage regimens included the following: maximal concentration and area under the concentration-time curve were higher at 11 mg/kg, once daily, than at 5.5 mg/kg, twice daily; and, more importantly, the ratio of AUC(0-24) to the minimal inhibitory concentration (MIC) value of 0.5 μg/mL for a 24 h period (AUC(0-24)/MIC) was higher when clindamycin was administered at 11 than at 5.5 mg/kg, at least during the first day of drug administration. Therefore, a better pharmacokinetic profile may be expected when clindamycin is administered at 11 mg/kg, once daily, for the treatment of canine pyoderma caused by Staphylococcus pseudintermedius. 相似文献
262.
Use of frozen mussel (Mytilus galloprovincialis) and mussel meal in the diet of sea bass (Dicentrarchus labrax Linnaeus, 1758) and sea bream (Sparus aurata Linnaeus, 1758) fingerlings – a preliminary study 下载免费PDF全文
263.
Eleni Nasiou Ioannis O. Giannakou 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(2):415-430
The protein kinase HOG1 in high osmolarity glycerol pathway is one of mitogen-activated protein kinases and plays an important role in fungi cellular responses to external stress and stimuli. In this study, AlHOG1, a yeast HOG1 homolog, was isolated from tobacco pathogenic fungus Alternaria longipes and its disrupted mutants were generated using homologous recombination. Compared with the wild-type strain, the mutants grew slower under Minimal-Media, produced less conidia on Potato-Dextrose-Agar and showed a retardation phenotype in conidial germination rate. Colony color observation indicated that AlHOG1 most likely negatively regulated melanin production under nutrients-deficient or osmotic stress. The same action mode of AlHOG1 on hyphal growth in response to certain carbon or nitrogen sources was speculated. Additionally, the disrupted strains were sensitive to osmotic and oxidative stress, and showed increased resistance to dicarboximide, phenylpyrrole and carbendazim fungicides. Meanwhile, results from the comparative analysis between the AlHOG1- and the AlHK1 (encoding a group III histidine kinase)-disrupted mutants showed that AlHOG1 mediated-pathway was partly regulated by the upstream AlHK1 kinase for cellular resistance to salts osmotic stress and that AlHK1 was a primary regulator for cellular sugar and fungicides stress adaptation and likely modulated both AlHOG1-mediated pathway and other unknown mechanisms. Noteworthily, AlHOG1 but not AlHK1 was confirmed to be associated with high concentration glycerol adaptation, which is the first report to our knowledge. Overall, our results indicated that AlHOG1 was involved in multiple physiological processes in A. longipes, including mycelial and conidial development, melanin production, nutrients sensing and multi-stress responses. 相似文献
264.
Ola Al Naddaf Ioannis Livieratos Aristides Stamatakis Ioannis Tsirogiannis George Gizas Dimitrios Savvas 《Scientia Horticulturae》2011
Composted pig manure (CPM), unmixed or in mixture with perlite, was evaluated as growing medium based on both physical characteristics and agronomic performance. The physical properties were determined in CPM, perlite (0.5–2.5 mm), and their 75:25, 50:50 and 25:75 mixtures by volume. It was found that unmixed CPM has a higher bulk density (BD) than perlite, a slightly lower air space at a suction of 10 cm, and a markedly higher content in easily available water (EAW), although the water content at a suction of 10 cm is the same in the two media. Mixing CPM with perlite by 50:50 seems to increase the actual water content and decrease the actual air content at container capacity, while the BD and the EAW score intermediate to those in the unmixed constituents. Simulation of the relative hydraulic conductivity (Kr) indicates a sharp decrease in the unsaturated hydraulic conductivity (K) of CPM, perlite and their mixtures as the media suction increases. The differences in the decrease rate of Kr between the tested substrates are rather small, with the most and least steep decrease being observed in perlite and the mixture by 50:50, respectively. The agronomic performance of CPM was assessed by growing greenhouse cucumber in bags filled with perlite up to 20 cm in height, CPM up to 10 or 20 cm in height, and their mixture by 50:50 up to the same heights as CPM. A media height of 10 cm in the bag resulted in appreciably higher yields than a height of 20 cm, regardless of the type of the substrate, while the latter had no significant effect on yield. This effect of media height was ascribed to the steep decrease in K, which was minimized in all media at higher suction levels than 10 cm, thereby severely restricting water availability in their upper layers (10–20 cm) when their height in the bag was 20 cm. 相似文献
265.
It has been studied the time course of seven leaf nutrients’ (Mn, Fe, Zn, Ca, Mg, K and P) concentrations when plants of the olive cultivar ‘Koroneiki’ were grown for about 5 months (from the 30th of May till the 17th of October) in three soils from different parent materials (Marl, Gneiss schist and Peridotite), located in the region of Central Macedonia, Northern Greece. 相似文献