The effect of genetic variation and nitrogen fertilization on productive characters of Greek oregano

This study aims to investigate the response of 10 Greek Oregano (Origanum vulgare subsp. hirtum) populations, collected across Greece, under 3 levels of N-fertilization during 2012 and 2013. The populations were differentiated for dry matter (from 32.7 to 63.3?g·pot^?1), for essential oil content (from 2.31 to 5.86?ml·100^?1?g dry weight) and for amount of essential oil (from 1.37 to 2.46?ml·pot^?1), for both years. Those with the highest dry matter (1-“Palaiochori” and 5-“Gliki”) and the highest essential oil content (10-“Gytheio” and 9-“Achladocastro”) were superior by 30%, as compared to the general mean of the experiment. The populations 3-“Litochoro” and 5-“Gliki” had an increased amount of essential oil (24%) as compared to the general mean of the experiment. An additional nitrogen supply (N₀ to N₁ level) increased dry matter production and amount of essential oil per pot, while decreased essential oil content for all populations. Further increase of nitrogen supply, favored six populations for dry matter production and two populations for amount of essential oil per pot while had almost no effect on five populations for essential oil content. The superior populations could be used in a breeding program as starting material for the development of new cultivars.     

Copper Binding by Activated Biochar Fibres Derived from <Emphasis Type="Italic">Luffa cylindrica</Emphasis>

The adsorption of copper (Cu(II)) from aqueous solutions by activated Luffa cylindrica biochar fibres has been investigated by means of batch equilibrium experiments and FTIR spectroscopy. The effect of various physicochemical parameters, such as pH, initial metal concentration, ionic strength, mass of the adsorbent, contact time and temperature, has been evaluated by means of batch type adsorption experiments. FTIR spectroscopy, as well as acid-base titrations, was used for the characterization of the material and the surface species formed. According to the experimental results even at pH 3, the relative sorption is above 85% and the adsorption capacity of the activated biochar fibres for Cu(II) is q _max = 248 g kg^?1. Moreover, the interaction between the surface carboxylic moieties and Cu(II) results in the formation of very stable inner-sphere complexes (?G ^o = ?11.2 kJ mol^?1 at pH 3 and ?22.4 kJ mol^?1 at pH 5.5).     

Salt tolerance evaluation of nine indigenous Greek olive cultivars

The present work was aimed to evaluate nine Greek olive (Olea europaea L.) cultivars for tolerance to sodium chloride (NaCl) salinity, most of which have never been evaluated or considered to be extinct, in order to be used in the future as olive rootstocks in areas with salt toxicity problems.One-year old, self-rooted trees of the cultivars ‘Aetonicholia Kynourias,’ ‘Arvanitolia Serron,’ ‘Ntopia Atsicholou,’ ‘Koroneiki,’ ‘Lefkolia Serron,’ ‘Ntopia Pierias,’ ‘Petrolia Serron,’ ‘Smertolia,’ and ‘Chrysophylli’ were subjected to 0, 50, 100, and 200 mM NaCl, for six months, in nutrient solution pot experiment. The experiment was conducted as a completely randomized factorial design with four replications (of one tree), with the factors being the cultivar (nine levels) and the salt concentration (four levels). According to plant growth parameter results, ‘Arvanitolia Serron’ followed by ‘Lefkolia Serron’ were found to be the most salt tolerant cultivars to growth reduction. The same cultivars accumulated less sodium (Na) and chlorine (Cl) in the leaves by retaining more ions in the roots. Moreover, ‘Arvanitolia Serron’ was able to maintain high potassium (K) levels and K/Na ratio in younger leaves under salinity.     

Response of two pomegranate (Punica granatum L.) cultivars to six boron concentrations: Growth performance,nutrient status,gas exchange parameters,chlorophyll fluorescence,and proline and carbohydrate content

One-year-old, own-rooted pomegranate cultivars “Ermioni” and “Wonderful” plants were irrigated for 75 days with modified Hoagland nutrient solutions containing 0–10 mg L^?1 boron (B). At the end of the experiment, the control plants of “Ermioni” presented better growth performance than those of “Wonderful.” However, there were no differences in the treatments with high B concentration (5.0 or 10 mg L^?1). Control “Wonderful” plants had higher fresh and dry matter than control “Ermioni” plants. Moreover, the highest B concentrations in nutrient solution led to a significant increase in chlorophyll and carbohydrate content in the leaves of cultivar “Ermioni.” Furthermore, leaf proline concentration, gas exchange, chlorophyll fluorescence, and micro–macronutrients of both cultivars were not affected by any of the tested B treatments. B concentration in plant parts was linearly correlated to B supply. The highest B concentrations were observed in roots followed by stems and apical and basal leaves.     

Identifying recreational fisheries in the Mediterranean Sea through social media

The impact of recreational fishing on fish stocks remains largely unknown, as this is inherently difficult to monitor, especially in areas such as the Mediterranean Sea where many species are targeted using a variety of fishing gears and techniques. This study attempts to complement existing data sets and construct the profile of recreational fisheries in the EU‐Mediterranean countries using videos publicly available on social media. A total of 1526 video records were selected, featuring the capture of 7799 fish specimens. The results show recreational fishing is multispecies in nature (26 species contributed to >80% % of the most numerically important species caught) and exhibits a spatially homogeneous pattern, with differences in species composition being mostly dependent on the fishing technique used rather than on the country. Such findings fill an important knowledge gap on recreational fishing activities, and the methodology provides an innovative approach to gather statistics on data‐poor thematic areas that can potentially complement other data sets, such as the EU Data Collection Multi‐Annual Programme.     

Elemental Relationships and Growth and Development of Spinach Grown with Sewage Sludge

A pot experiment with spinach (Spinacia oleraceae L. Fam.: Chenopodiaceae) was conducted aiming to investigate the effect of the addition of sewage sludge (SS) on soil substrate, the growth of spinach, and the plant interactions of CaxCd and ZnxCd. There were six substrates obtained by mixing soil and sludge in different proportions by volume (20:1, 10:1, 6.7:1, 5.0:1, 4.0:1, 3.3:1) and a control (only soil). The highest biomass was achieved in treatments 20:1 and 10:1. There was not a linear increase due to toxicity when SS was added in higher proportions. Sludge improved soil fertility by increasing organic matter and total N. Furthermore, in all plant parts of spinach, the Ca and Zn contents were synergistically interrelated with the Cd content, facilitating Cd uptake. The use of sewage sludge as soil amendment is not applicable on agricultural land due to the accumulation of Cd in plant.     

Gastro-oesophageal reflux in large-sized,deep-chested versus small-sized,barrel-chested dogs undergoing spinal surgery in sternal recumbency

Objective

The aim of this study was to investigate whether an increased frequency of gastro-oesophageal reflux (GOR) is more common in large-sized, deep-chested dogs undergoing spinal surgery in sternal recumbency than in small-sized, barrelchested dogs.

ASSESSMENT OF SENTINEL LYMPH NODE METASTASIS IN CANINE MAMMARY GLAND TUMORS USING COMPUTED TOMOGRAPHIC INDIRECT LYMPHOGRAPHY

In the staging process of the breast cancer, demonstrating metastasis of the sentinel lymph node (SLN) has an important prognostic value, in both humans and animals. The aim of this prospective case‐control study was to determine the diagnostic value of computed tomographic indirect lymphography (CT‐LG) for detecting SLN metastasis in dogs with mammary cancer. Thirty‐three female dogs with tumors in the abdominal and inguinal mammary glands were prospectively selected and subjected to CT‐LG, 1 and 5 min after injection of 1 ml of contrast agent (iopamidol) in the subareolar tissue of the neoplastic and the contralateral normal mammary glands. The pattern of postcontrast opacification, degree of postcontrast enhancement, and size and shape were assessed in 65 SLNs in total and were correlated with histopathological findings. The absence of opacification or heterogeneous opacification 1 min after contrast medium injection showed the highest sensitivity, specificity, and accuracy (93%, 100%, and 98.4%, respectively). In images taken 1 min after injection, an absolute density value lower than 444 Hounsfield units (HU) in the center of the SLN also provided significant sensitivity and specificity (93.8% and 75%, respectively). The size and shape of the SLN (maximum and minimum diameter, maximum/minimum diameter ratio, maximum diameter/height of fifth thoracic vertebral body ratio) showed the lowest sensitivity and specificity. Results of this study support the hypothesis that CT‐LG could help in the assessment of SLN metastasis in cases of mammary gland tumors in dogs.     

Optimizing diquat efficacy with the use of adjuvants

Optimizing diquat efficacy with the use of adjuvants may broaden the spectrum of weed control, but relevant research towards this direction is limited. Field and greenhouse experiments were conducted to evaluate the effect of diquat applied alone and with six commercial adjuvants (surfactants and oil-based adjuvants) on various weed species. Diquat effect was evaluated in two field experiments on natural populations of common lambsquarters (Chenopodium album L.), prostrate knotweed (Polygonum aviculare L.) and burning nettle (Urtica urens L.) along with two greenhouse trials on rigid ryegrass (Lolium rigidum L.). In field or greenhouse experiments, all the adjuvants significantly increased the control of C. album, P. aviculare, and L. rigidum, from 48, 42 and 7%, up to 82, 74 and 67%, respectively, in terms of fresh weight reduction, but to a different extent for each adjuvant. U. urens was totally (100%) controlled in terms of visual estimation either with diquat or with diquat plus any adjuvant. The differences in the effect of diquat applied with adjuvants mainly depended on the weed species examined and they were not proportional to the surface tension reduction of the spray solution caused by the adjuvants. Overall, the surfactants and the oil-based adjuvants examined in this study considerably enhanced the effect of diquat; this can broaden the spectrum of weed control against broadleaf and grass weeds in orchards and non-crop areas. The results are discussed in relation with the classification of the adjuvants.     

Effect of geraniol,a plant-based alcohol monoterpene oil,against <Emphasis Type="Italic">Meloidogyne javanica</Emphasis>

In continuing previous work on the role of AAA+ proteins of the apple proliferation agent ‘Candidatus Phytoplasma mali’ in phytoplasma virulence and suppression of virulence, 147 full-length deduced protein sequences of AAA+ ATPase AP460 of single- and multiple-strain accessions were examined. This approach revealed that in this protein two regions can be distinguished. Region 1, located in the N-terminal part, is characterized by 14 highly conserved substitutions associated with the suppression of virulence. However, these substitutions were not present in all attenuated strains. In the more diverse region 2, located in the C-terminal part, highly conserved substitutions associated with two groups of virulence were identified. In addition to the virulence-related residues, three other groups of conserved substitutions are present in strains with attenuated virulence with or without the presence of suppression-associated substitutions in region 1. In one of these groups, substitutions next to key residues of the ATPase motifs sensor 1 and 2 and arginine finger do occur that seem to affect ATPase function. This group of substitution was present in all attenuated strains. From these findings it can be concluded that two different mechanisms of suppression exist of which the supposed effect on ATPase function seems to be more important than the suppression-associated substitutions in region 1. The presence of virulent, avirulent and suppressive strains in a tree leads in the commonly occurring multiple infections to interactions in which the resulting virulence is determined by the numerical relation of virulent and attenuated strains.