Diazinon resistant status in Rhipicephalus (Boophilus) microplus collected from different agro-climatic regions of India

The resistance status of Rhipicephalus (Boophilus) microplus to "Diazinon" was evaluated in 20 locations situated at various agro-climatic regions of India. Adult immersion test (AIT) was optimized using laboratory reared acaricide susceptible IVRI-I strain of R. (B.) microplus and minimum effective concentration of Diazinon was determined as 635.2 ppm. The discriminating dose (DD) was worked out as 1270.4 ppm and was tested on female ticks collected from organized and unorganized farms located at different agro-climatic regions of India. On the basis of the data generated on three variables viz., mortality, egg masses and reproductive index, the resistance level was categorized as I, II, III and IV. The average resistance factor (RF) of 6.1 (level II) was recorded in the ticks collected from the northern sub-temperate trans-gangetic plains while high average RF values of 26.65 (level III) was recorded in the ticks collected from tropical middle-gangetic plains. The tropical middle gangetic plain has a very high density of animal populations where farmers use Diazinon for tick control, for agricultural practices and for mosquito control. Due to the continuous use of OP compounds the environmental load of Diazinon has become high in the area. This is the first experimental data generated on Diazinon resistant status in ticks of India.     

Bioavailability of iron from wheat aegilops derivatives selected for high grain iron and protein contents

A coupled in vitro digestion/Caco-2 model was employed to assess iron bioavailability from wheat Aegilops derivatives selected for high iron and protein contents. The iron content in wheat genotypes used in this study correlated to a great extent with both protein (r = 0.80) and phytate (r = 0.68) contents. The iron bioavailability was based on Caco-2 cell ferritin formation from cooked digests of these derivatives (relative to WL711 control) and correlated positively with dialyzable iron (r = 0.63) and total iron content (r = 0.38) but not with the phytate content. The apparently decreased phytate/iron molar ratios, however, correlated negatively (r = -0.42) with the iron bioavailability, justifying the utilization of these parameters in biofortification programs. Iron bioavailability in the derivatives increased up to 1.5-fold, corresponding to a 1.5-2.2-fold increase observed in iron content over control. These data suggest that biofortification for iron proportionately leading to higher iron bioavailability will be the most feasible and cost-effective approach to combat micronutrient deficiency.     

Characteristics of life-form and growth-form of plant species in an alpine ecosystem of North-West Himalaya

The present study was conducted in the alpine pastures of Tungnath (30° 14’ N and 79° 13’ E) to observe life-form and growth-form patterns of alpine plant species under grazed and ungrazed conditions and to work out the plant life form spectrum. Species were categorized as plant habit, height and length of growth-cycle and life-form classes according to Raunkiaer’s system. The results show that in total of 68 species at grazed site, hemicryptophytes (He) accounted for 50.00% species, followed by cryptophytes (26.47%), chamaephytes (16.18%), phanerophytes (4.41%) and therophytes (2.94%). At the ungrazed site in 65 plant species, hemicryptophytes (He) accounted for 49.23% species, cryptophytes (26.15%), chamaephytes (15.38%), phanerophytes (6.15%) and therophytes (3.08 % species). In general, hemicrptophyte are dominant in both sites i.e. graged and ungrazed. Growth form categories were classified as forbs, shrubs, grasses and sedges and undershrubs, according to plant habit and height. On the basis of length of the growth cycle, species were categorized as plant species of short growth cycle, intermediate growth cycle and long growth cycle. The short forbs of plant habit and height, had the highest emergence, and grasses and sedges had the lowest emergence in representative species. Percentage of species with long growth cycle was highest in both sites.     

Composting of Tropical Toxic Weed Lantana camera L. Biomass and Its Suitability for Agronomic Applications

ABSTRACT

Lantana camara is an evergreen, which is the most notorious toxic weed of the terrestrial ecosystem. It is native to subtropical and tropical America, but a few taxa are indigenous to tropical Asia and Africa. An enormous quantity of green foliage is produced by this weed, which cannot be used as livestock feed due to its toxic properties. Management through utilization seems the only sustainable option for this problem. In this study, the composting of Lantana biomass was done and changes in chemical characteristics of waste biomass were measured. The composting caused decreases in pH, organic carbon, C:N ratio _totK and _totC by 2.0-, 1.25-, 1.66-, and 19-fold, respectively, but increases in electrical conductivity (EC), ash content, _totN, _totP, _totZn, and _totMg of 2.0-, 1.11-, 3.36-, 1.76-, 1.28-, and 1.70-fold, respectively. The C/N ratio (20.1) and soil respiration rate (47.12–66.20 mg CO₂-C/100 g) suggested the compost maturity at 52 days. The high bacterial (38.67 CFU × 10^?7 g^?1), fungal (30.0 CFU × 10^?3 g^?1), and actinomycetes (32.0 CFU × 10^?5 g^?1) population in composted material suggested the suitability of compost for agronomic purposes. Phytotoxity measured through compost:water extract and compost pot trial suggested the germination index (GI) in the ranges of 52.3%–122.3% and 74.5%–166.9%, respectively. The high ranges of chlorophyll, protein, and carotenoids in seedling than control suggested the non-toxicity of ready materials. Results suggested that composting can be a potential technology to manage Lantana biomass for sustainable land fertility management programs.     

Towards sustainable production of peppermint (Mentha piperita L.) through integrated use of vermicompost and cowpea green manuring with synthetic nitrogen fertilization

A field experiment with peppermint (Mentha piperita L.) was conducted in a sandy loam (Typic ustifluvent) soil during 2007 and 2008 at Lucknow, India. Ten treatments consisting of control (no synthetic or organic nitrogen fertilization), synthetic nitrogen fertilization (SN) 75, 150 and 225 kg ha^?1 alone, vermicompost (VC) 3 t + 37.5 kg SN ha^?1, VC 6 t + 75 kg SN ha^?1 and VC 9 t + 112.5 kg SN ha^?1 and intercropping of one, two and three rows of cowpea for green manuring in combination with 50, 100 and 150 kg SN ha^?1, respectively, were evaluated in a randomized block design. Integrated use of VC 9 t with 112.5 kg SN ha^?1 produced maximum essential oil (94.3 kg ha^?1), increased the herb and essential oil yields by 104 and 89%, respectively, over control and reduced SN use by 50%, without affecting the quality of essential oil. Application of VC and intercropping of cowpea for green manuring significantly improved the organic carbon, available N, P and K content in soil over SN alone. To get sustainable production of peppermint, application of VC 9 t ha^?1 along with 112.5 kg N ha^?1 through synthetic fertilizer is recommended for light textured sandy loam soils.     

Effect of seed mass on emergence and seedling development in Pterocarpus marsupium Roxb.

We investigated the effect of seed mass on emergence, seedling survival and growth ofPterocarpus marsupium Roxb., a medium to large, commercially valuable and deciduous tree species. Among the three size classes viz. small （10-12 mm）, medium （13-15 mm） and large （16-17 mm）, the maximum proportion, of seed by number （54.12%） and dry weight （51.87%） was recorded, in the medium size seed class. Seed length and seed width were greatest in the large seed class （16.50 mm, 7.33 mm） followed by medium （13.50 mm, 5.60 mm） and small （11.37 ram, 3.66 mm）. Similarly, hundred seed weight （100 sw） varied from a maximum of 12.92 g in the large seed class to intermediate 10.95 g in the medium seed class and minimum of 7.02 g in the small seed class. The large seed size showed maximum emergence and shoot length over the medium and small seed class. After six months of growth, significant variations due to seed size were also observed for the growth and dry weight of P. marsupium seedlings. Seedling vigour, expressed in terms of height, collar diameter, number of leaves and dry biomass, was sig- nificantly affected by seed class. Seedlings that emerged from large seeds showed better growth and produced heavier seedlings as compared to medium and small seeds.     

Assessment of Losses of Hybrid Sorghum ‘CSH-1’ to Earhead Caterpillars,and Comparison of Insecticidal Controls

Abstract

In hybrid sorghum ‘CSH-1’ severe infestation of the earhead caterpillars Cydia sp., Ectomyelois sp., Eublemma sp. nr. gayneri Roths., Heliothis armigera Hub., and Euproctis limbata Wlk, was recorded at Jabalpur (Madhya Pradesh) during 1968. The first two were relatively more serious. The loss in grain yield was estimated to be 18.26 per cent, i.e. about 717 kg/ha.

Among different insecticides tested, carbaryl 0.2 per cent spray proved most effective, followed by endosulfan 0.05 per cent spray. Dichlorvos 0.05 per cent gave good initial kill, but showed little persistent effect.     

Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene

Treatment of bovine mastitis caused by Staphylococcus (S.) aureus is becoming very difficult due to the emergence of multidrug-resistant strains. Hence, the search for novel therapeutic alternatives has become of great importance. Consequently, bacteriophages and their endolysins have been identified as potential therapeutic alternatives to antibiotic therapy against S. aureus. In the present study, the gene encoding lysin (LysSA4) in S. aureus phage SA4 was cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clone revealed a single 802-bp open reading frame encoding a partial protein with a calculated mass of 30 kDa. Results of this analysis also indicated that the LysSA4 sequence shared a high homology with endolysin of the GH15 phage and other reported phages. The LysSA4 gene of the SA4 phage was subsequently expressed in Escherichia coli. Recombinant LysSA4 induced the lysis of host bacteria in a spot inoculation test, indicating that the protein was expressed and functionally active. Furthermore, recombinant lysin was found to have lytic activity, albeit a low level, against mastitogenic Staphylococcus isolates of bovine origin. Data from the current study can be used to develop therapeutic tools for treating diseases caused by drug-resistant S. aureus strains.     

Studies on some Drought Resistant Traits of Pearl Millet Cultivars and their Association with Grain Production under Natural Drought

A field experiment was conducted to evaluate the performance of hybrid (BJ 104, MBH 110, CM 46 and GHB 27) and composites (PSB 3, PSB 8, WCC 75 and Local) of pearl-millet on the soils (pH 7.1, Organic carbon 0.5 %, P₂O₅ 9.6 kg/ha, K₂O 80 kg/ha, soil depth 65 cm and profile moisture 8.6 cm) of Indian Grassland and Fodder Research Institute, Jhansi under extreme drought condition (340 mm total precipitation during the crop season). It was observed that hybrids, by and large, gave higher production than composites and local variety even failed to bloom. Further the highest yield from hybrid MBH 110 was due to its drought escaping character (earliness), drought enduring capacity (CSI-12), efficient translocation and better sink strength. BJ 104 and CM 46, though were also drought escaping and possessed capacity to endure drought but inferior to MBH 110 in sink strength which was ultimately reflected in production.
An appraisal of the various agro-physiological traits revealed that earliness, high harvest index, low CSI value (chlorophyll stability index) and high sink strength were directly correlated with productivity of cultivars under drought condition which could be used as a guide lines for breeding drought resistant cultivars of pearl millet.