Proton magnetic resonance spectroscopic method for determination of phenylbutazone and oxyphenbutazone in tablets

A simple, specific, and rapid 1H nuclear magnetic resonance spectroscopic method for the assay of phenylbutazone and oxyphenbutazone is described. Spectra are recorded in CDCI3 containing 1,3-dichloro-5-nitrobenzene as an internal standard. The aromatic proton resonances for the standard, at delta 7.7 and 8.2, are well separated from those of phenylbutazone and oxyphenbutazone, which are in the region of delta 6.5-7.3 ppm. Average percent recoveries of phenylbutazone and oxyphenbutazone were 98.9 and 98.6 with standard deviations of 0.6 and 0.7, respectively. Commercial formulations were analyzed and the results obtained by the proposed method closely agreed with those found by the British Pharmacopoeia method.     

Folacin and cyanocobalamin in relation to naturalTrypanosoma evansi Infection in buffaloes

Summary The haematological values of haemoglobin (Hb), packed cell volume (PCV) and vitamin levels of folacin (folic acid), cyanocobalamin (vitamin B₁₂) of buffaloes, in a surra endemic area of Eastern Haryana, India, were determined. Surra-positive buffaloes had significantly low levels of Hb, PCV, folic acid and vitamin B₁₂. These low levels of folic acid and vitamin B₁₂ may have enhanced the clinical signs of chronic trypanosomosis, caused byTrypanosoma evansi.

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Folacina Y Cianocobalamina En Relacion Con La Infeccion Natural De Bufalos ConTrypanosoma Evansi

Resumen Se determinaron los valores hematológicos de hemoglobina (Hb), volumen corpuscular celular (PCV) y los niveles vitamínicos de ácido fólico y cianocobalamina (vitamina B₁₂) de búfalos, en un área endémica de surra en Haryana Oriental, India. Los búfalos positivos a surra tuvieron niveles bajos de Hb, PCV, ácido fólico y vitamina B₁₂. Los niveles bajos de ácido fólico y cianocobalamina, pudieron haber exacerbado los sintomas clinicos de tripanosomiasis crónica, causada porTrypanosoma evansi.

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Folacine Et Cyanocobalamine En Relation Avec La Trypanosomose ATrypanosoma Evansi Chez Des Buffles

Résumé Les valeurs hématologiques de l'hémoglobine (Hb), l'hématocrite (PCV) et les taux de la folacine (acide folique) et de la cyanocobalamine (vitamine B₁₂) chez des buffles ont été déterminés dans une région de l'Est Haryana, Inde, où sévit le surra à l'état endémique. Les buffles positifs vis-à-vis du surra présentaient des taux significativement faibles de Hb, PCV, d'acide folique et de vitamine B₁₂. Les taux peu élevés de ces deux derniers éléments peuvent renforcer les siqnes cliniques de la trypanosomose chronique, due àT. evansi.

     

谈谈家蚕化水蛹的发生与防治

化水蛹,又称为黄死蛹或化骨龙,是家蚕后期死蛹的一种,该病在蚕种生产上发生较为普遍。每年每造均有不同程度的发生,其中以高温造(3～5造)发生较多,给蚕种生产带来一定程度的损失。现在根据我们的一些试验调查和生产中的一     

Ultrastructural alterations in the kidneys of Pekin ducks fed methylmercury.

Ultrastructural alterations in the kidneys of Pekin ducks were studied after subchronic exposure to methylmercury chloride. Twenty-four male and twenty-four female Pekin ducks, 12 weeks of age were fed 0.0 (control), 0.5, 5.0 and 15.0 parts per million of MeHgCl, mixed in a mash diet for 12 weeks. Birds were observed for clinical signs daily. Kidneys were fixed in situ by perfusion with 4% glutaraldehyde in 0.05 M phosphate buffer and routinely processed for electron microscopy. Only minor morphological changes were observed in the renal corpuscle of ducks in the 0.5 ppm group. The component cells of the renal corpuscle in the 5.0 and 15.0 ppm groups showed several ultrastructural alterations, including an increase in number and size of lysosomes and vacuoles in podocytes and mild vacuolation in mesangial cells. The juxtaglomerular cells of afferent arterioles had crystallized granules. No significant changes were observed in glomerular basement membrane thickness and width of podocyte pedicels. The proximal tubules of the 5.0 and 15.0 ppm groups showed some microvillar loss and an increase in the number and size of lipid droplets, vacuoles and lysosomal bodies. The distal tubular changes in the 5.0 and 15 ppm groups included electron lucency and vacuolation of cytoplasm as well as occasional mitochondrial swelling. The collecting ducts of 5.0 ppm and 15.0 ppm groups showed varying degrees of degenerative changes in both mucus secretory as well as absorptive cells. These results suggest that subchronic exposure of Pekin ducks to methylmercury chloride causes subtle ultrastructural changes in renal corpuscle and marked ultrastructural changes in proximal tubules and collecting ducts.