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651.
652.
653.
M C Healey H H Hwang Y Y Elsner A V Johnston 《Canadian journal of veterinary research》1991,55(2):121-127
The objective of this study was to demonstrate that a field isolate of Actinobacillus seminis (As8C) will adhere to epithelial cells and that this adhesion can be inhibited by pretreating the bacteria with mouse serum containing polyclonal antibodies (PoAbs) prepared against this isolate. An indirect fluorescent antibody test, transmission electron microscopy, and phase-contrast microscopy confirmed the adhesion of As8C to an established culture of bovine kidney epithelial cells (BKECs). In a bacterial adhesion assay, 40 As8C were estimated to adhere to each BKEC after 60 min. Using a bacterial inhibition assay, PoAbs diluted 10(-2) or 10(-3) inhibited the adhesion of As8C to BKECs by approximately 90%. Bacterial inhibition decreased to about 50% when the PoAbs were diluted to 10(-4). There was less than 10% inhibition of adhesion of As8C to BKECs when higher dilutions of PoAbs were used. The inhibition of As8C adhesion to BKECs was less than 20% following pretreatment of BKECs with 10(-2) to 10(-5) dilutions of PoAbs. Moreover, pretreatment of As8C with a 10(-2) dilution of PoAbs did not appear to adversely affect bacterial growth on agar. It is likely that the PoAbs interrupted the adhesion of As8C to BKECs by sterically interfering with a bacterial adhesin-epithelial cell receptor interaction. 相似文献
654.
Wook-Hun Chung Jae-Hoon Lee Dai-Jung Chung Wo-Jong Yang A-Jin Lee Chi-Bong Choi Hwa-Seok Chang Dae-Hyun Kim Hyo Jin Chung Hyun Jung Suh Soo-Han Hwang Hoon Han Sun Hee Do Hwi-Yool Kim 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(3):329-335
Here, percutaneous spinal cord injury (SCI) methods using a balloon catheter in adult rats are described. A balloon catheter was inserted into the epidural space through the lumbosacral junction and then inflated between T9-T10 for 10min under fluoroscopic guidance. Animals were divided into three groups with respect to inflation volume: 20 µL (n = 18), 50 µL (n = 18) and control (Fogarty catheter inserted but not inflated; n = 10). Neurological assessments were then made based on BBB score, magnetic resonance imaging and histopathology. Both inflation volumes produced complete paralysis. Gradual recovery of motor function occurred when 20 µL was used, but not after 50 µL was applied. In the 50 µL group, all gray and white matter was lost from the center of the lesion. In addition, supramaximal damage was noted, which likely prevented spontaneous recovery. This percutaneous spinal cord compression injury model is simple, rapid with high reproducibility and the potential to serve as a useful tool for investigation of pathophysiology and possible protective treatments of SCI in vivo. 相似文献
655.
Seonah Lee Jeongyeon Hwang Hyunwook Kim Yeonjung Hong Gahyun Lee Doori Chung Jaehwan Kim Kidong Eom 《Veterinary radiology & ultrasound》2023,64(2):233-242
An improved understanding of the CT characteristics for histologically confirmed primary intestinal tumors would be helpful for guiding prognosis and treatment plans in affected dogs. This retrospective, multi-center, analytical study aimed to evaluate the CT characteristics for the differentiation of adenocarcinoma, lymphoma, and spindle cell sarcoma (SCS) in dogs. Thirty-seven dogs who underwent contrast CT and histopathological examinations were included (adenocarcinomas, n = 11; lymphomas, n = 12; SCS, n = 14). Quantitative and qualitative CT parameters, including tumor morphology, contrast enhancement pattern, Hounsfield unit (HU) value, and presence or absence of intraabdominal lymphadenopathy, were evaluated for each included small intestine tumor CT case. Adenocarcinomas tended to show endophytic growth, intestinal obstruction, and a heterogeneous enhancement pattern. Lymphomas tended to show exophytic growth, contrast enhancement of the intestinal tumor mucosal layer, a homogeneous enhancement pattern, and the presence of lymphadenopathies in the abdominal cavity. SCSs tended to show lobulated growth, a large cystic portion within the tumor, a heterogeneous enhancement pattern, a large size with fat stranding sign, and lower HU values in postcontrast images. Cut-off values of the minimum diameter/fifth lumbar vertebral mid-body height (≥5.80; area under the curve [AUC] = 0.97, P < 0.001) and minimum HU value/HU value of the aorta (≤0.26; AUC = 0.96, P < 0.001) were derived to discriminate SCS from the two other tumor types. In conclusion, contrast CT characteristics may be useful in differentiating small intestinal adenocarcinomas, lymphomas, and SCSs in dogs. 相似文献
656.
Avin Arjoonsingh DVM Jillian M. Haines DVM MS DACVIM Julianne K. Hwang PhD Sarah C. Guess DVM MS DACVIM K. Jane Wardrop DVM MS DACVP 《Journal of Veterinary Emergency and Critical Care》2023,33(6):656-664
Objective
To assess storage lesion development, platelet function, and bacterial growth in canine platelet concentrates (PCs) stored in a platelet additive solution (PAS) or a plasma control at 4°C for 21 days.Design
Prospective, ex vivo, experimental controlled study.Setting
University veterinary teaching hospital.Animals
Ten units of canine PCs collected from blood bank donations.Interventions
The PCs were separated into 2 bags, 1 containing 100% plasma and the other containing 35% plasma and 65% of a PAS (Plasma-Lyte A), and stored at 4°C for 21 days. At days 0, 7, 14, and 21, PCs were analyzed for the presence of swirling, aggregate formation, platelet counts, platelet indices, glucose, lactate, lactate dehydrogenase, Pvco 2, Pvo 2, aggregation via light aggregometry, activation percentages using flow cytometry, and bacterial growth.Measurements and main results
Cold-stored PCs in both PAS and plasma control maintained mean pH >6.8 and mean lactate <9.0 mmol/L over 21 days, with no difference in glucose utilization. Swirl was maintained in both solutions for most days (76/80 combined total samples), with no difference in aggregate formation between solutions. The Pvco 2 was higher in plasma on all days (P < 0.001), with no difference in Pvo 2. Platelet indices did not reflect significant storage lesion development in either solution. Lactate dehydrogenase did not differ between solutions but did increase from day 7 to day 21. Mean maximal aggregation percentage was reduced overall but with no significant difference between solutions. The only observed difference in mean activation percentage between solutions was in PAS on day 7, which was significantly higher than plasma (P < 0.05). No bacterial growth occurred during storage.Conclusions
Cold storage in PAS and plasma allowed PCs to be stored for up to 21 days with minimal storage lesion development, maintenance of platelet function, limited platelet activation, and no bacterial growth within stored bags. 相似文献657.
Seo KW Choi US Jung YC Hong SJ Byeun YE Kang MS Pachrin B Kim WH Hwang CY Kim DY Youn HY Lee CW 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):201-204
A 10-year-old Maltese dog was presented with abdominal distention and dyspnea. Cytological examination of pleural and peritoneal effusion was suggestive of malignant effusion of glandular origin. Numerous, multifocal, tan to white nodules were disseminated throughout the surface of the abdominal organs and peritoneum at biopsy. Histologically, the tumors were revealed to be an epithelial type of mesothelioma. Neoplastic cells co-expressed cytokeratin and vimentin. Intravenous administration of cisplatin was chosen as the treatment. During treatment, the dog's overall body condition improved and the clinical signs were relieved without significant side effects. The survival time from diagnosis to sudden death by unknown cause was 153 days. 相似文献
658.
Park HM Hwang DN Kang BT Jung DI Song GS Lee SJ Yhee JY Yu CH Doster AR Sur JH 《Veterinary pathology》2007,44(6):921-923
We describe a 10-month-old, intact female American Cocker Spaniel with pulmonary lymphomatoid granulomatosis (PLG). On clinical examination, this dog presented with nonproductive dry cough, serous nasal discharge, dyspnea, and lack of appetite. Radiography showed a consolidated lesion in the left cranial lung lobe. Histopathologic examination showed a mixed population of atypical lymphoid cells that had infiltrated into the pulmonary blood vessels angiocentrically. The lymphocytes were CD3 positive, consistent with a pan-T-cell phenotype. The lymphoid cells in the lesion were also positive for CD20cy and CD79a, indicative of the presence of B cells. We also observed large Reed-Sternberg-like cells that were positive for CD15 and CD30, similar to observations in human pulmonary Hodgkin's disease (PHD). In conclusion, canine PLG in this Cocker Spaniel was associated with B and T cells, which is first identified in a case of canine PLG. It was histopathologically similar to human lymphomatoid granulomatosis and immunophenotypically similar to human PHD. 相似文献
659.
Edelstein AS Chow GM Altman EI Colton RJ Hwang DM 《Science (New York, N.Y.)》1991,251(5001):1590-1592
An unusual distribution of particle sizes has been observed following the formation of molybdenum particles by argon ion sputtering. Many of the molybdenum particles produced by sputtering at the threshold pressure for particle formation in the vapor appear to be single crystalline cubes. There are two prominent peaks in the edge length distribution of the cubes, one centered at 4.8 nanometers with a halfwidth of approximately 1.3 nanometers and the other at 17.5 nanometers. The peak for the larger cubes is approximately square and has a total width of 7.0 nanometers. Evidence is presented that the larger cubes are formed by a 3 by 3 by 3 self-arrangement of the smaller cubes, which contain approximately 7000 atoms. Self-arrangement in inorganic structures is normally only observed when the building blocks are atoms, molecules, or clusters of less than 100 atoms. 相似文献
660.
Shiliang Liu Xiaolu Jiang Xiaoke Hu Jun Gong Hueymin Hwang & Kangsen Mai 《Aquaculture Research》2004,35(7):678-682
The effect of chronic (2 weeks) temperature (19, 22, 25, 28 and 31°C) on certain non‐specific immune parameters of two species of scallops, Argopecten irradians (Lamarck 1819) and Chlamys farreri (Jones & Preston 1904) were studied. The survival ratio of the two species of scallops at 31°C was the lowest. Haemocyte concentration of C. farreri in the 19 and 22°C treatment groups was significantly lower than that of the 25 and 28°C treatment groups but significantly higher than that of the 31°C treatment group. With elevation of seawater temperature, the alkaline phosphatase (ALP) and acid phosphatase (ACP) activities in sera of two scallop species increased and reached the peak at 25°C and then decreased. However, the specific activity of ALP and ACP of the 31°C treatment group was the highest. The results demonstrated that temperature of seawater significantly affected immunity of scallops. The concept that the stress of high environmental temperature on C. farreri may be partially responsible for the mass mortality of the organism is also introduced. 相似文献