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111.
AIM:To study the changes of K+ channels of outer hair cells in guinea pig cochlea with streptomycin ototoxicity. METHODS:Auditory brainstem responses (ABR) and whole-cell patch clamp techniques were used.RESULTS:(1) The body weight of guinea pigs with streptomycin ototoxicity decreased significantly; (2) The ABR threshold markedly increased in streptomycin group (Ⅱ,Ⅲ);(3)The number of dissociated outer hair cells of guinea pigs (Ⅱ,Ⅲ) was lower than that of control (Ⅰ); (4) Streptomycin decreased the Ca2+-sensitive K+ currents and delayed outward K+ currents distinctly; (5) There was no significant difference of K+ currents between Ⅰ and Ⅱ/Ⅲ. CONCLUSION:These results suggest that the inhibition of K+ channels is the basis of streptomycin ototoxicity, but not the direct reason for cell death. 相似文献
112.
AIM:To determine the effects of Angiotensin II(AngII) on migration of rat smooth muscle cells and to investigate the mechanisms underlying Ang II action in the development of injured vascular disease. METHODS:VSMCs isolated from aortic media of Wistar rats and cultured by the modified explant method were adopted. In prersence and absence of AngII, the expression of AngII receptor and reorganization of the actin cytoskeleton of VSMCs were studied by immunocytochemistry technique, fluorocytochemistry technique. The migration assays were performed by a modified Boyden's chamber. And the effects of AT1R antagonist (CV-11974), AT2R antagonist (PD123319) on aforementioned target were studied.RESULTS:VSMCs migration was stimulated by addition of AngII. The dynamic reorganization of actin cytoskeleton may be an important mechanism by which AngII facilitates VSMC motility. The expression of AT1R in VSMCs can be upregulated after treatment with AngII initially, then decreased gradually. The expression of AT1R was downregulated by AT1R antagonist. The effect of AngII on VSMCs migration was mediated by AT1R, while AT2R had no significant effect.CONCLUSION:The dynamic reorganization of actin cytoskeleton is required for AngII-induced VSMC migration, and this effect is mediated by AT1R . 相似文献
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Y. J. Huang C. Toscano-Underwood B. D. L. Fitt † X. J. Hu A. M. Hall 《Plant pathology》2003,52(2):245-255
Ascospores of both A-group and B-group Leptosphaeria maculans germinated at temperatures from 5 to 20°C on leaves of oilseed rape. Germination of ascospores of both groups started 2 h after inoculation and percentage germination reached its maximum about 14 h after inoculation at all temperatures. Both the percentage of A-/B-group ascospores that had germinated after 24 h incubation and germ tube length increased with increasing temperature from 5 to 20°C. Germ tubes from B-group ascospores were longer than those from A-group ascospores at all temperatures, with the greatest difference at 20°C. Hyphae from ascospores of both groups penetrated the leaves predominantly through stomata, at temperatures from 5 to 20°C. A-group ascospores produced highly branched hyphae that grew tortuously, whereas B-group ascospores produced long, straight hyphae. The percentage of germinated ascospores that penetrated stomata increased with increasing temperature from 5 to 20°C and was greater for A-group than for B-group L. maculans after 40 h incubation. 相似文献
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在鉴定苏云金芽孢杆菌(Bacillus thuringiensis,简称Bt)Btc001菌株cry基因型的基础上,构建了Btc001菌株质粒DNA HindⅢ片段的文库,并利用聚合酶链式反应-限制性酶切片段多态性(PCIR-RFIP)方法筛选出含有crylC6全长基因的13.5kb大片段,酶切分析得到该片段的物理图谱,BamHI和EcoRI切完成了6.5kb含全长基因的亚克隆,并对这条6.5kb片段亚克隆、测序,序列在国际核酸序列数据库(GenBank)登记(AY007686),并由Bt杀虫晶体蛋白基因国际命名委员会命名为cry1Cb2基因。根据序列设计了一对用于扩增全长基因的引物S581CB和S381CB,扩增产物插入表达载体pET-21b中,诱导后在大肠杆菌BL21(DE3)中获得高效表达。表达产物对小菜蛾(Plutella xylostella)表现出较高活性,LC50达到7.9μg/ml。 相似文献
118.
20世纪80年代后,稻瘿蚊发生危害明显回升,损失相当严重。以大田试验示范为主,系统研究了新耕作条件下稻瘿蚊综合防治,首次总结提出“喷药除草、压低虫源、抓住晚秧、做好测报、挑治本田”的综合防治新技术体系。室内外试验和大田示范表明,应用草甘膦杀死越冬寄主游草,可显著减少虫源基数;灭线磷(商品名益舒宝)、氯唑磷(商品名米乐尔)是防治稻瘿蚊的高效药剂,抗蚊青占品种高抗稻瘿蚊。此外,合理利用杀虫植物、性引诱剂和天敌等亦是防治稻瘿蚊的重要措施。在面积达12.46万hm^2的示范推广实践中,本田防治效果均达80%以上。 相似文献
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采用 4 0英尺集装箱熏蒸处理纸箱 ,实验对箱中溴甲烷气体分布及散气后溴甲烷残留进行了检测研究。结果表明 ,温度高低、集装箱中纸箱堆放和电扇鼓风等情况对溴甲烷分布与扩散影响明显。集装箱装满纸箱情况下 ,当温度≥ 2 1℃ ,投药 2h后上、下检测点平均溴甲烷浓度达到最低浓度要求 ;当温度 <2 1℃时 ,投药 2 4h后上检测点平均溴甲烷浓度仍达不到最低浓度要求。当集装箱中间留有通道或使用电扇鼓风情况下 ,投药 2h后各检测点溴甲烷浓度分布达到最低浓度要求。集装箱装满纸箱情况下 ,溴甲烷向大气扩散较慢 ,即使电扇鼓风散气2h ,2 4h后溴甲烷残留量仍达不到安全指标要求。集装箱中间留有通道情况下 ,箱中溴甲烷残留量下降很快 ,并可达到安全指标要求。 相似文献
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