Septicaemia secondary to infection by Corynebacterium macginleyi in an Indian python (Python molurus)

A seven-year-old female Indian python (Python molurus) weighing about 35kg was euthanased after several clinical episodes of stomatitis, pneumonia, ophthalmitis and dystocia over a period of four years. The animal had been maintained in a terrarium in a circus truck at an adequate temperature. During shows, however, the snake was considered to be exposed to stressful conditions for several hours at a time at low temperatures and with noise and bright lights. A post-mortem examination indicated ulcerative stomatitis, osteomyelitis, severe pneumonia and numerous granulomata and multifocal necrosis in stomach and spleen. Corynebacterium macginleyi was isolated in pure culture from the ulcerative stomatitis, and mixed with Stenotrophomonas maltophilia from the lungs and spleen. The findings indicated that the snake had died from a septicaemic process caused by C. macginleyi, probably originating from the stomatitis. The role of S. maltophilia as a secondary agent is discussed. The stress of the circus show and poor husbandry may have predisposed the animal to infection and septicaemia. This is the first report of C. macginleyi causing disease in a snake.     

两种蔗糖浓度对切割取样鼠胚冷冻解冻效果的研究

小鼠晚期桑椹胚、早期囊胚和扩大囊胚，经切割取样分别采用０．３６５Ｍ和０．２５Ｍ蔗糖浓度进一步细管和分步脱除甘油解冻后体外培养发育率分别为６５．５％、４６．４％和７５．４％，两种浓度间差异显著（Ｐ＜０．０５）；分步脱除甘油解冻瑟前者差异不显著（Ｐ＞０．０５），与后者差异极显著（Ｐ＜０．０１）。     

猪圆环病毒2型感染对猪繁殖与呼吸综合征灭活疫苗体液免疫的影响

本试验采用ELISA方法对单独接种猪繁殖与呼吸综合征病毒(PRRSV)变异株灭活苗组(HP组,n=3)和PCV2感染且出现病毒血症后接种PRRSV变异株灭活苗组(PCV2/HP组,n=3)不同时相血清中的PRRSV抗体进行检测;并对HP和PCV2/HP组血清中PCV2特异的抗体和核酸分别进行ELISA和荧光定量PCR检测。结果表明,在首免后70 d HP组血清平均抗体效价极显著高于PCV2/HP组(P<0.01);首免后56、63和77 d HP组平均抗体效价明显高于PCV2/HP组。其中在首免后56和63 d HP组抗体阳性率均达67%(2/3),PCV2/HP组在相应时相抗体阳性率为0;在首免后70和77 d HP组抗体阳性率均达100%(3/3),PCV2/HP组在相应时相抗体阳性率仅为0和33%(1/3)。结果提示PCV2感染可在一定程度上抑制PRRSV变异株(JXA1)特异性的抗体反应。     

非洲猪瘟自然弱毒株演变进程

自2018年非洲猪瘟(African swine fever,ASF)传入中国以来疫情得到了有效控制.但2021年以后,我国的ASF流行情况出现了新的变化,临床中出现了"自然变异株".这些毒株可能导致感染后临床症状不典型,容易与其他疫病混淆等问题.这与国外的流行演变规律一致,即当ASF在一个国家或地区流行较长时间后,其...     

犬冠状病毒在犬体内分布的初步研究

对电镜检查粪便中发现有犬冠状病毒的 4只犬 ,进行了血常规、尿常规、肝肾功能和心电图检查 ,均未发现明显异常。扑杀后经电镜负染检查 ,除肠内容物外 ,在其肝、心、脾、肾和肺等实质脏器中均发现冠状病毒粒子     

In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus

In this work the antiviral activity of 20 dehydroepiandrosterone (DHEA) analogs with different substituents at positions C-3, C-15, C-16 and C-17 were evaluated against vesicular stomatitis virus (VSV) in Vero cell cultures. The selectivity indexes (SI) obtained with DHEA and epiandrosterone (EA) were 50 and 72.6, respectively. The work showed that the compounds 21-norpregna-5,17(20)-dien-3β,16α-diyl-diacetate, 17,17-ethylendioxyandrostan-5,15-dien-3β-ol and 3β-hydroxypregn-17(20)-en-16-one had higher SI values than ribavirin, which was used as a reference drug. The antiviral mode of action of DHEA was also investigated against VSV replication in Vero cells, and time of addition experiments showed that DHEA mainly affected a late event in the virus growth cycle. Analysis of RNA and protein synthesis indicated that DHEA adversely affected positive strand RNA synthesis and viral mature particle formation.     

Direct lateral transmission of Histomonas meleagridis in turkeys

The lateral transmission of Histomonas meleagridis in turkeys was studied in floor pens without the presence of Heterakis gallinarum. Battery-reared poults (120) were transferred at 2 wk of age to concrete-floored floor pens with fresh pine shavings litter (40/group). One group received no exposure. In other groups, either 10% or 25% of the birds were inoculated per cloaca with cultured H. meleagridis (200,000/bird) and placed in the pens as seeder birds. Inoculated birds died at 10-18 days postinfection (PI) showing typical liver and cecal lesions of histomoniasis. Birds in the high-exposure group died of histomoniasis beginning 16 days PI and continuing to 100% mortality by day 23 PI. Birds in the low-exposure (LE) group died beginning on day 19 PI and continuing through day 31 PI. All but one LE bird alive on day 31 PI had severe liver and cecal lesions of histomoniasis at necropsy. There was no evidence of histomoniasis in unexposed birds. No cecal worms (H. gallinarum) were found at necropsy of dead birds or in unexposed birds at the end of the experiment. Even though H. gallinarum is the only known reservoir for H. meleagridis, these results suggest that lateral transmission of histomoniasis through a flock can occur readily through normal contact between uninfected birds and infected birds and their droppings in the total absence of cecal worms.     

静注克洛素隆在绵羊体内的药代动力学研究

应用YWG-C18色谱柱(250 mm×4.6 mm,5μm),WatersTM 486型可调波长紫外检测器(检测波长265 nm);0.01 M磷酸钾(pH=7)乙腈(3 1)为流动相;含量测定采用标准曲线法,建立了RP-HPLC法检测绵羊血浆中克洛素隆含量的方法.同时对绵羊单剂量静脉注射7 mg/kg克洛素隆的药代动力学进行了研究.血药浓度在0.005～2.0 μg/ml及2.0～50.0μg/ml范围呈良好线性关系(r=0.9941、0.9970),平均回收率93.2%,血药最低检测浓度0.005μg/ml,日内日间变异系数分别小于10%、11%.血药浓度结果经MCPKP药代动力学统计软件处理,体内药物运转符合三室开放模型,主要药物动力学参数t1/2γ=14.14 h,Kel=0.3260 h-1,AUC=153.95 mg/l@h,fc=0.055.结果表明克洛素隆静脉单次给药后体内分布较为广泛,持续作用时间长,主要通过肾脏排泄.     

青海省牛、羊棘球蚴病流行病学调查

为了解青海省家畜棘球蚴病流行现状,给防治工作提供科学依据,2011年,在不同自然环境条件下选择5个县(市)对牛、羊棘球蚴病进行了流行病学调查.结果表明,5县牛、羊棘球蚴平均感染率和感染强度分别为27.09％、33.89％和0.5、1.3,犬细粒棘球绦虫平均阳性率为10.91％.     

The Relationship Between the Positive Identification of the Embryo Proper in Equine Pregnancies Aged 18-28 Days and its Future Viability: A Field Study

Early embryonic loss (EEL) negatively affects the reproductive efficiency of equine reproduction. A sign of future EEL is when the embryo proper (EP) fails to develop within the embryonic vesicle after 30 days of gestation. The earlier the identification of impending EEL, the earlier the mare can be rebred to allow a second chance of pregnancy. The objectives of this study were to determine the percentage of embryonic vesicles with a visible EP at 18-28 days of gestation and to study the association between the presence/absence of the EP at different days of gestation and the future viability of the pregnancy. A total of 1,256 pregnancies were identified and followed by transrectal B-mode ultrasonography 12-45 days post ovulation in mares of the same Thoroughbred farm. The identification of the EP was attempted once during days 18-28. Pregnancy reconfirmation was performed on days 35-45. The percentage of vesicles with an EP increased gradually from days 18 (2.8%) to 21 (86.9%) (P < .05). From day 20 onward, the EEL rate of mares with vesicles without an EP was significantly higher (P < .05) than that of vesicles with a positive identification of an EP. In conclusion, the EP of the equine vesicle can be identified reliably with B-mode ultrasonography in the majority of mares (>71%) on day 20 of gestation. The lack of a positive identification of an EP from day 20 onward is associated with a higher EEL rate.