Release and activity of allelochemicals from allelopathic rice seedlings

3-Isopropyl-5-acetoxycyclohexene-2-one-1 (1), momilactone B (2), and 5,7,4'-trihydroxy-3',5'-dimethoxyflavone (3) were isolated and identified from an allelopathic rice accession PI312777. These three compounds at low concentrations could inhibit the growth of weeds Echinochloa crusgalli and Cyperus difformis associated with rice, especially mixtures of the compounds had stronger inhibitory activity than did individual compounds. Studies with hydroponic culture, continuous root exudates trapping system (CRETS), and direct resin adsorption methods showed that a total of 7.6 n moles 1, 2, and 3 were exuded from living roots of each seedling into the environment at 10 days after seedlings were transplanted. Furthermore, 1, 2, and 3 were found in the soil growing PI312777 seedlings at day 15 after seedlings emergence and reached a total of 39.5 microg/g soil at day 30. The results indicated that PI 312777 seedlings could release sufficient quantities of 1, 2, and 3 into the environment to act as allelochemicals inhibiting the growth of associated weeds. Investigations on the distribution of 1, 2, and 3 in PI 312777 plant, and its root exudates showed that the levels of 1, 2, and 3 were significantly higher in the shoots and root exudates than in the roots, and only trace 1 was observed in the roots. The results suggest that the roots of rice seedlings are not major site of synthesis or accumulation 1, 2, and 3, but a pathway for their release into the environment. The levels of 1, 2, and 3 in the root exudates were over 2-folds higher under direct resin adsorption than under hydroponic culture and CRETS, and hence, it is the preferred method to collect and identify active allelochemicals in rice exudates in future studies on rice allelopathy.     

黄土区不同下垫面农田降雨入渗及产流关系的数值模拟

在野外试验的基础上，建立了二维农田降雨入渗及产流关系的数学模型，并用中国科学院长武县王东沟农业生态站不同下垫面径流小区实测土壤含水率及产流资料对该模型进行验证。结果显示：该模型对降雨过程中土壤入渗、产流以及雨后蒸发条件下土壤水分的模拟都有很高的精度，可以用于黄土区降雨入渗及产流关系的模拟。     

青藏高原农田生态系统碳平衡

为探讨青藏高原农田生态系统碳平衡规律及其影响因子 ,并揭示其对全球含碳温室气体源、汇变化的贡献与响应 ,1 998～ 1 999连续 2年在拉萨农业生态站农田用静态箱法和生物量取样法开展土壤—植被界面CO2 吸收与排放的试验研究。农作物生长季 ,以冬小麦为例 ,生长前期 (从播种到拔节 )土壤碳排放量高于植被碳固定 ,生长后期 (灌浆到成熟 )则是植被碳固定高于土壤碳排放量 ,从全年来看 ,土壤碳排放量略高于植被碳固定     

硒从土壤向食物链的迁移

硒是生物必需的微量营养元素 ,已经得到公认 ,硒从土壤向食物链的迁移状况直接关系到动物和人的健康。通过食物链 ,动物和人获得适合于健康需求的硒是最为安全和可行的     

淋洗对盐胁迫下大豆生长和矿质营养基因型差异的研究

植物质外体是由细胞壁的纤维素和微晶体空间以及充满空气的细胞间隙组成 ,约占植物体积 5 %的空间。很久以来 ,质外体被认为是无生命的 ,与有生命的原生质相比很少引起研究者的兴趣 ,直到 80年代中期 ,由于研究技术的发展和研究的深入[1 ] ,对质外体的认识才发生根本性的转变 ,     

镉污染土壤植物修复的EDTA调控机理

我们通过温室盆栽试验 ,在不同Cd处理的土壤中加入EDTA ,分析了印度芥菜根和地上部Cd的浓度 ,探讨EDTA进入土壤后对Cd吸收和运输的影响。结果表明 :加入EDTA ,水提取的Cd浓度增加了 40 0倍以上 ,NH4 NO3提取的Cd浓度增加了 40倍以上 ,在土壤Cd浓度较低时 ,EDTA对植物吸收Cd没有显著影响 ,当土壤添加Cd在 1 3 0mgkg- 1以上时 ,加入EDTA显著增加了地上部Cd的浓度。EDTA能增加印度芥菜地上部中Cd的浓度 ,不是由于土壤溶液中Cd浓度增加从而增加了印度芥菜根对Cd的吸收 ,可能是EDTA加入土壤后增加了这些元素在土壤溶液中的浓度 ,从而高浓度的Cd对植物根细胞产生毒害 ,增加了细胞膜的透性后 ,土壤溶液中的络合物得以进入根细胞并随蒸腾作用运输到地上部。     

Quantification of the group B soyasaponins by high-performance liquid chromatography

High-performance liquid chromatographic methods were developed for the isolation and quantitative determination of the group B soyasaponins, including 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated soyasaponins alphag, betag, and betaa, and their non-DDMP counterparts, soyasaponins V, I, and II, respectively, with formononetin used as the internal standard. The limits of quantification for soy products were 0.11-4.86 micromol/g. The within-day and between-days assay coefficients of variation were <9.8 and < 14.3%, respectively. The group B soyasaponin concentrations in 46 soybean varieties ranged from 2.50 to 5.85 micromol/g. Soy ingredients (soybean flour, toasted soy hypocotyls, soy protein isolates, textured vegetable protein, soy protein concentrates, and Novasoy) and soy foods (commercial soy milk, tofu, and tempeh) contained the group B soyasaponins from 0.20 to 114.02 micromol/g. There was no apparent correlation between isoflavone and soyasaponin concentrations in the soy products examined.     

银杏叶提取物微胶囊化研究

为使银杏叶提取物能在食品领域得到充分有价值的应用，探索采用微胶囊技术来解决银杏类黄酮难溶于水及强烈苦味的问题。本研究选用阿拉伯胶和β-环状糊精作为银杏黄酮微胶囊壁材，通过喷雾干燥进行微胶囊化。结果表明：选用阿拉伯胶和β-环状糊精(1∶1)作为银杏黄酮的微胶囊壁材，能达到较好的微胶囊化效果，且芯材与壁材的最佳配比为0.1 g/g。最佳喷雾干燥工艺条件为：进料固形物浓度为25％，进风温度为180℃，进料速度为35 mL/min。产品微胶囊化效率达90％以上。     

作物根系分区交替灌溉田间实施技术探索

作物根系分区交替灌溉方法的提出开辟了节水灌溉的新思路,该方法具有明显的节水、增产、提高水分利用效率的优点.目前对它的田间实施技术研究还不全面、不系统.本文针对其特点,探索分析能够实现根系分区交替灌溉目的的灌水技术种类、技术要点及适用条件,提出适合目前我国农村经济发展现状的根系分区交替灌溉田间实施技术,并对未来的技术发展提出了研究思路.     

多效唑对天津水蜜桃生长发育影响的研究

对长势旺产量较低的天津水蜜桃进行了叶面喷洒多效唑(PP_(333))营养生长抑制剂的研究.结果表明,新梢生长受抑的程度与多效唑的浓度呈正相关.叶绿素的含量与一定浓度的多效唑处理具有密切的关系.较高浓度和高浓度的乡效唑处理可提高果实中维生素C的含量、单果重以及果品质量,而对果实中可溶性固形物、果实硬度、着色指数和单叶面积无影响.