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121.
Multiclonal origin of polyps in Gardner syndrome 总被引:4,自引:0,他引:4
Electrophoretic analysis of glucose-6-phosphate dehydrogenase was performed on polyp tissue from three black female patients with Gardner syndrome and who are heterozygous for the A and B forms of this enzyme. Polyp tissues from the three patients displayed the AB phenotype. This finding suggests a multiclonal origin of polyps in Gardner syndrome. Studies of tumors originating from such polyps may provide information about the sequence of cellular events leading to malignant transformation. 相似文献
122.
Hsu KF Loo S Guo F Chen W Dyck JS Uher C Hogan T Polychroniadis EK Kanatzidis MG 《Science (New York, N.Y.)》2004,303(5659):818-821
The conversion of heat to electricity by thermoelectric devices may play a key role in the future for energy production and utilization. However, in order to meet that role, more efficient thermoelectric materials are needed that are suitable for high-temperature applications. We show that the material system AgPb(m)SbTe(2+m) may be suitable for this purpose. With m = 10 and 18 and doped appropriately, n-type semiconductors can be produced that exhibit a high thermoelectric figure of merit material ZTmax of approximately 2.2 at 800 kelvin. In the temperature range 600 to 900 kelvin, the AgPb(m)SbTe(2+m) material is expected to outperform all reported bulk thermoelectrics, thereby earmarking it as a material system for potential use in efficient thermoelectric power generation from heat sources. 相似文献
123.
Germline transmission of exogenous genes in the chicken 总被引:7,自引:0,他引:7
R A Bosselman R Y Hsu T Boggs S Hu J Bruszewski S Ou L Kozar F Martin C Green F Jacobsen 《Science (New York, N.Y.)》1989,243(4890):533-535
Difficulties associated with in vitro manipulation and culture of the early chicken embryo have restricted generation of transgenic chickens to approaches that use replication-competent retroviruses. The need to produce transgenic chickens in the absence of replicating virus prompted development of a new method of gene transfer into the chicken. Microinjection of the replication-defective reticuloendotheliosis virus (REV) vector ME111 beneath unincubated chicken embryo blastoderms results in infection of germline stem cells. This vector contains genetic information exogenous to the chicken genome, including both the herpes simplex virus type 1 thymidine kinase gene and the Tn5 neomycin phosphotransferase gene. About 8 percent of male birds hatched from injected embryos contained vector DNA in their semen. All four positive males tested passed vector sequences onto their progeny. Analysis of G1 offspring showed that gonads of G0 male birds were mosaic with respect to insertion of vector provirus. Thus, primordial germ cells present in the unincubated chicken embryo blastoderm are susceptible to infection by defective REV vectors. 相似文献
124.
泸沽湖自然保护区森林植被及木本植物区系 总被引:4,自引:0,他引:4
泸沽湖自然保护区位于云南省宁蒗县境内,北纬27°36′~27°47′,东经100°43′~100°54′,海拔2680~3870m,这里至今还居住着保留母系家庭结构的摩梭人。根据生态——外貌学的方法,本区的森林植被可划分为6个植被类型和11个群系。云南松、苍山冷杉、丽江云杉、高山栎类是本区森林的主要建群树种。其木本植物区系特征主要表现在下列几方面。(1) 种系密度较小,每平方公里1.06属1.88种;(2) 温带性质明显,是泛北极区系的一部分,温带成分占67.7%;(3) 区系成分多样,与周围地区联系广泛,本区有12个分布类型8个变型,与热带成分的联系以泛热带和热带亚州成分较为密切;(4) 起源古老;(5) 特有现象明显,中国,西南,云南特有种分别为45、65、15种;(6) 喜钙或钙生性种类较多,占总种数的85%。 相似文献
125.
Zinc deficiency in rats caused decrease in the activity of pancreatic carboxypeptidase A, but it had no effect on pancreatic carboxypepti dase B and liver alcohol dehydrogenase. The observed reduction of enzymic activity may be related to the poor utilization of feed that occurs in zincdeficient rats. 相似文献
126.
Wang PH Hsu CI Tang SC Huang YL Lin JY Ko JL 《Journal of agricultural and food chemistry》2004,52(9):2721-2725
FIP-fve is a fungal immunomodulatory protein purified from Flammulina velutipes, an edible golden needle mushroom thought to possess potent immunomodulatory properties. When examined for its effects on lymphocytes, FIP-fve exhibited potent mitogenic effects on human peripheral blood lymphocytes, inducing G1/G0 to S phase proliferation. T cells activated by FIP-fve show significant production and secretion of interferon-gamma (IFN-gamma) associated with intercellular adhesion molecule 1 expression but low detectable levels of interleukin-4 in vitro or in vivo. However, SB203580, the p38 mitogen-activated protein kinase (p38 MAPK) inhibitor, can fully abolish the production of IFN-gamma induced by FIP-fve. At the same time, SB203580 only partially prevents the lymphocytes from progressing from G1 to S phase of the cell cycle. These findings demonstrate that FIP-fve is a potent T-cell activator, mediating its effects via cytokine regulation of p38 MAPK. The immunoprophylatic effects of FIP-fve in Th2-mediated allergic anaphylaxis are believed to be associated with the ability of FIP-fve to enhance activation of IFN-gamma-releasing Th1 cells. 相似文献
127.
Solutions of 100 mL of 1% commercial pectin each with a different degree of esterification (DE), DE94, DE65, and DE25, were reacted with 100 mL of 2 M alkaline hydroxylamine (pH 12.0) at room temperature for 4 or 18 h. These pectin hydroxamic acids (PHAs; DE94T4, DE94T18, DE65T4, and DE25T4) were used to test the inhibitory activities against semicarbazide-sensitive amine oxidase (SSAO) and angiotensin-converting enzyme (ACE). Compared to different DE pectins (DE94, DE65, and DE25), the PHAs of DE94T4, DE94T18, DE65T4, and DE25T4 showed different inhibition activities against SSAO or ACE. Commercial pectins with different DE values showed negligible SSAO or ACE inhibitions. The order of SSAO inhibition was DE65T4 > DE94T18 approximately DE25T4 > DE94T4. However, the order of ACE inhibition was DE94T4 > DE94T18 > DE65T4 > DE25T4. The SSAO activity staining or ACE-hydrolyzed products on TLC chromatogram also confirmed the inhibitory activities of PHAs against SSAO or ACE. 相似文献
128.
129.
Shi-Yen Shiau Hao-Ling Yu Sophy Hwa Shih-Yang Chen Song-Ing Hsu 《Aquaculture (Amsterdam, Netherlands)》1988,70(4):345-354
Carboxymethylcellulose (CMC) was incorporated into each of two experimental diets at one of four levels (2, 6, 10 and 14%). Each of the eight diets was fed to male tilapia hybrids (Oreochromis niloticus × O. aureus) for 9 weeks. The experiment was conducted in a closed, filtered and recirculated rearing system. The fish were fed the appropriate diets at a rate of 3% of body weight per day, 2 times a day, 6 days a week. The water temperature was maintained at 26°C. Tilapia fed diets containing, 6, 10 and 14% CMC showed significantly poorer (P<0.05) feed conversion rates than fish fed diets containing 2% CMC. The dry matter digestion coefficients of the diets did not differ significantly but generally declined as CMC level increased. Gastric emptying times decreased as the level of inclusion of CMC in the diets increased. Crude protein content and ash content of fish were similar in all dietary groups. Fish fed diets containing 6, 10 and 14% CMC had significantly lower (P<0.05) lipid content than fish fed diets with 2% CMC. 相似文献
130.
Haemocytes of the giant freshwater prawn, Macrobrachium rosenbergii, were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non-pathogenic latex beads in vitro. Isolated haemocytes of M. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast Debaryomyces hansenii, the bacteria Aeromonas hydrophila or Enterococcus faecium, or with latex beads at 25 degrees C for 2 h, followed by washing to remove free particles. At least 200 haemocytes were counted to determine the phagocytosis rate, and the results showed that haemocytes engulfed latex beads at a higher rate than the aquatic pathogens. By transmission electron microscopy, the yeast- or bacterium-engulfing haemocytes displayed morphological changes characteristic of apoptosis, including formation of cytoplasmic vacuoles, chromatin condensation and fragmentation of nuclei. This pathogen-induced apoptosis was further confirmed by DNA laddering and TUNEL (terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end-labelling) assays. Neither haemocytes treated with latex beads nor uninfected haemocytes (control group) showed signs of apoptosis after 48 h in culture. 相似文献