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11.
12.
Proliferation and collapse of subcuticular hyphae of Venturia nashicola race 1 were studied ultrastructurally, after inoculation of susceptible Japanese pear cv. Kousui, resistant Japanese pear
cv. Kinchaku, resistant Asian pear strain Mamenashi 12 and nonhost European pear cv. Flemish Beauty leaves, to understand
the nature of the resistance mechanism. After cuticle penetration by the pathogen, the hyphae were observed at lower frequency
in epidermal pectin layers and middle lamellae of leaves of the three resistant plants than in those of susceptible ones.
This result suggested that fungal growth was suppressed in the incompatible interaction between pear and V. nashicola race 1. In the pectin layers of all inoculated plants, some hyphae had modifications such as breaks in the plasmalemma with
plasmolysis, necrotic cytoplasm and degraded cell walls. More hyphae had collapsed in the leaves of the three resistant plants
than in those of the susceptible cv. Kousui. In collapsed hyphae, the polymerized cell walls broke into numerous fibrous and
amorphous pieces, showing that the scab resistance might be associated with cell wall-degrading enzymes from pear plants. 相似文献
13.
Hideo Hamaguchi Naoki Yamamoto Akinori Takeda Takehiro Masumura Tetsushi Azuma 《Soil Science and Plant Nutrition》2020,66(2):299-307
ABSTRACT We compared the protein and oil contents, seed yields, and natural abundance of 15N (σ15N) of seeds from the plants of the cultivar Enrei, which has moderate nodulating ability (Enrei) with those of its two near-isogenic lines (NILs), a super-nodulating genotype of Enrei, i.e., En-b0-1, and a non-nodulating genotype of Enrei, i.e., En1282. Plants of these three genotypes were grown on four types of field plots with different types of urea coated slow-release nitrogen (N) fertilizers (CUSLNFs) which delivered N in different manners during plant growth . The seed yield of the En1282 plants was similar to that of the Enrei plants but much greater than that of the En-b0-1 plants when the plants were grown on the field to which a CUSLNF with a long lifespan was applied. The amounts of protein per seed were positively correlated with those of oil per seed in the case of En1282 plants irrespective of the field N conditions. The seed protein contents were proportional to the seed weight in both En1282 and Enrei plants. Such a relationship was not observed in the En-b0-1 plants, and the protein and oil contents in the seeds of En-b0-1 plants were negatively correlated with each other. These observations suggested that the N supply to maturing seeds was a key to the protein contents in the En1282 and Enrei plants and that the C supply to maturing seeds was a key to the protein contents in the En-b0-1 plants. The comparison of the σ15N values, protein contents, and seed yields of seeds from En1282 plants suggested that the Enrei plants assimilated considerable amounts of N from the soil during the late plant growth. We concluded that high N2 fixation activity depressed the amounts of oil per seed and changed the protein and oil contents in soybean seeds. 相似文献
14.
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16.
Osamu Yamato Eun-Og Jo Toru Shoda Masahiro Yamasaki Yoshimitsu Maede 《Journal of veterinary diagnostic investigation》2004,16(5):469-472
This report describes a rapid and simple method for mutation screening of G(M1) gangliosidosis in Shiba dogs by direct amplification of DNA from canine whole-blood specimens using a novel polymerase chain reaction (PCR) reagent cocktail, which can eliminate the DNA extraction process and amplify the genomic DNA directly from human or murine whole blood. The strategy of this mutation screening is based on the identification of a nucleotide deletion by restriction enzyme analysis, coupled with the direct PCR amplification. The target sequence of the canine beta-galactosidase gene could be amplified directly from various forms of canine whole-blood specimens, including anticoagulated blood, blood stored frozen for 1 year, dried blood held in filter paper for 1 year at room temperature, and dry powder of blood stripped from Giemsa-stained blood films, which had been prepared 10 years earlier, resulting in the determination of genotypes in all the specimens. This method simplified the molecular diagnosis and carrier screening of G(M1) gangliosidosis in Shiba dogs, making it simple to examine specimens from the large, widely distributed population of these dogs. 相似文献
17.
Shingo Kawai Kyoko Okita Kazuhiro Sugishita Ai Tanaka Hideo Ohash 《Journal of Wood Science》1999,45(5):440-443
A modified synthetic method for phenolic-O-4 lignin substructure model dimers was developed involving protection of the phenolic hydroxyl group of acetophenons with benzoyl chloride, bromination with 4-dimethylaminopyridiniumbromide perbromide, condensation with phenols in the presence of 18-crown-6-ether, condensation with paraformaldehyde, reduction with NaBH4 and debenzoylation. This method results in shorter reaction times and increasing yields without the application of strict anhydrous and drastic conditions or chloric solvents. This alternative route could be applied to the-O-4 dilignol syntheses of four combinations of guaiacyl and syringyl derivatives. 相似文献
18.
The fatigue behavior of plywood specimens under shear through thickness was examined on the basis of strain energy to obtain
common empirical equations for the fatigue process and failure criterion under various loading conditions. Specimens were
cut from commercial plywood panels of 9-mm thickness. Loading conditions were set as follows: a square waveform at a loading
frequency of 0.5 Hz, a triangular waveform at 0.5 Hz, and a triangular waveform at 5.0 Hz. Peak stress applied was determined
to be 0.5, 0.7, and 0.9 of static strength, that is, stress levels of 0.5, 0.7, and 0.9. The stress-strain relationships were
measured throughout the fatigue test, and the strain energy was obtained at each loading cycle. Loading conditions apparently
affected the relationship between stress level and fatigue life. On the other hand, the relationship between mean strain energy
per cycle and fatigue life was found to be independent of loading conditions. Mean strain energy per cycle obtained as the
fatigue limit was 5.85 kJ/m3 per cycle. Assuming that the accumulation of strain energy is a fatigue indicator, the fatigue process and failure criterion
for the plywood specimens under the three loading conditions were commonly expressed by the relationship between cumulative
strain energy and loading cycles. 相似文献
19.
Kartika DEWI Hideo HASEGAWA Yuli Sulistya FITRIANA Mitsuhiko ASAKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1217-1222
The present report describes Syphacia
(Syphacia) maxomyos sp. n. (Nematoda:
Oxyuridae) from two species of spiny rats, Maxomys musschenbroekii from
Sulawesi and M. whiteheadi from Sumatra. It is characterized by a
cephalic plate extending laterally with dorsoventral constriction and stumpy eggs with an
operculum rim reaching pole. It is readily distinguishable by the former feature from all
of hitherto known representatives of this genus in Indonesia, but it resembles parasites
in Murini and Hydromyni rodents in continental Asia and Sahul. This is the first
Syphacia species distributed in both the Sunda Shelf and Sulawesi with
the exception of Syphacia muris, a cosmopolitan pinworm found in rodents
of the of genus Rattus. It is surmised that S. maxomyos
is specific to Maxomys and that it was introduced to Sulawesi by
dispersal of some Maxomys from the Sunda Shelf. 相似文献
20.
K. Nakamura T. Osuga K. Morishita S. Suzuki T. Morita N. Yokoyama H. Ohta M. Yamasaki M. Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(6):1746-1752