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Fisheries Science - Crustacean hemocytes, which have usually been classified morphologically based on methods using Giemsa or May-Giemsa stains, have recently been categorized using monoclonal... 相似文献
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Hirao H Inoue T Hoshi K Kobayashi M Shimamura S Shimizu M Tanaka R Takashima K Mori Y Noishiki Y Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):357-362
A new valved conduit was developed using a canine aortic valve. The bioprosthetic valve was fixed with glutaraldehyde and epoxy compound (Denacol-EX313/810). A vascular graft composed of ultra-fine polyester fiber (10 mm in diameter, 200 mm in length) was used. Four dogs underwent apico-aortic valved conduit (AAVC) implantation and aortic banding (bypass group, BG), while another 4 dogs underwent aortic banding without AAVC implantation (control group, CG). Cardiac catheterization and angiocardiography were performed for assessment of hemodynamics 2 weeks and 6 months after surgery. Left ventricular systolic pressure, left ventricular end-diastolic pressure and the left ventricular-aortic pressure gradient differed significantly (P<0.01) between the BG and CG dogs. Left ventricular angiocardiography showed patency of the valved conduit in all the BG dogs. Echocardiography was performed before and 2, 4 and 6 months after surgery, and showed that while pressure overload caused concentric myocardial hypertrophy in the CG dogs, the left ventricle dilated eccentrically in the BG dogs. Furthermore, relief of left ventricular pressure overload by AAVC was maintained. 相似文献
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Fukui Y Iwayama H Matsuoka T Nagai H Koma N Mogoe T Ishikawa H Fujise Y Hirabayashi M Hochi S Kato H Ohsumi S 《The Journal of reproduction and development》2007,53(4):945-952
The present study was conducted during the Kushiro Coast Survey in an attempt to produce common minke whale embryos. In Experiment 1, we attempted to determine the appropriate culture duration (30 or 40 h) for in vitro maturation (IVM) of immature oocytes using the Well of the Well method. In Experiment 2, and intracytoplasmic sperm injection (ICSI) was applied to matured oocytes from prepubertal and adult common minke whales after IVM culture (40 or 48 h), and then their embryonic development was assessed. In Experiment 1, the maturation rate of oocytes cultured for 40 h (30.4%) was significantly higher than that of oocytes cultured for 30 h (6.8%; P<0.01). In Experiment 2, a total of 35 and 46 immature oocytes derived from adult (n=2) and prepubertal (n=6) minke whales, respectively, were cultured for 40 or 48 h. The maturation rate in the oocytes from the adult whales (34.2%) tended to be higher than that of the oocytes from the prepubertal whales (19.6%), but there was no significant difference. Following ICSI, 3 out of the 10 inseminated and cultured oocytes from the adult whales cleaved (2-, 8-, and 16-cell stages); all of these oocytes had been matured for 40 in culture. However, these oocytes did not develop to further stages. Only one of the 6 oocytes derived from the prepubertal whales, IVM cultured for 40 h and inseminated, developed to the 4-cell stage. The present results indicate that a 40 h IVM culture produces significantly higher rates of in vitro maturation than a 30 h IVM culture for common minke whale oocytes. Following ICSI, some oocytes cleaved to the 16-cell stage, but no further development was observed. 相似文献
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Furukawa S Nagashima Y Hoshi K Hirao H Tanaka R Maruo K Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):41-44
In veterinary medicine, dopamine is currently being administered clinically by infusion for treatment of kidney disorders at low doses (< or = 3 microg/kg/min) and for assessment of hemodynamics at high doses (> or = 5 microg/kg/min). However, since high doses of dopamine cause peripheral vasoconstriction due to its effect on alpha adrenoceptors, high doses have no longer been recommended. The present study was conducted to explore possible regimens for the use of dopamine infusion in dogs. The regional (renal and cardiac) blood flow for 60 min was measured by using colored microspheres at three doses (3, 10 and 20 microg/kg/min) of dopamine infusion in healthy anesthetized mongrel dogs. The effects on kidney and peripheral hemodynamics at each dose and the resultant cardiac output, mean arterial blood pressure and total peripheral resistance were determined. Renal blood flow increased markedly at 3 microg/kg/min dopamine. Improvement in hemodynamics indicated by marked increase in cardiac blood flow, cardiac output and mean arterial blood pressure and decreased total peripheral resistance was observed at higher doses (10 and 20 microg/kg/min). At 10 microg/kg/min, in addition to the satisfactory increase in cardiac blood flow, there was also a stable satisfactory increase in renal blood flow. However, at 20 microg/kg/min, increased myocardial oxygen consumption (manifested by marked increased in cardiac output), arrythmia and irregular increase in renal blood flow were detected. This study suggests that the clinical use of dopamine infusion in dogs could be safely expanded to moderately higher doses. 相似文献
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Heat shock proteins (HSPs) are a group of highly conserved molecular chaperones. Shrimp HSPs have recently been a topic of increasing interest because of their roles in shrimp immunity and homeostasis. In penaeid shrimp, HSP70s and the cognate forms, heat shock cognate (HSC) 70s, have been reported, but their responses towards various stimulations are different. We found a novel type HSP70 (MjHSP70-2) from the hyperexpansion of the large segmental duplication that is present in kuruma shrimp Marsupenaeus japonicus, which shows about 60 % identity with reported shrimp HSP70s. In a phylogenetic tree, MjHSP70-2 formed a sister clade with eukaryote HSP70 family while MjHSP70 was located close to the shrimp HSP70 and HSC70 group. MjHSP70-2 gene expression was not significantly increased by heat shock or pathogen challenge by Vibrio penaeicida, but it was significantly increased by infection with white spot syndrome virus. In contrast, MjHSP70 gene expression was increased by heat shock but decreased by infection with V. penaeicida. The kuruma shrimp genome was found to have 400-fold more copies of the MjHSP70-2 gene than the putative single-copy gene transglutaminase. In conclusion, our results reveal the presence of a novel type HSP70 gene, HSP70-2, from kuruma shrimp. There are multiple forms of HSP70 in crustaceans, and these HSP70s behave differently under various stressors. 相似文献
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Hirao H Hoshi K Kobayashi M Shimizu M Shimamura S Tanaka R Machida N Maruo K Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):559-562
A three-month-old male Golden Retriever had symptoms including exercise intolerance, dyspnea and syncope and was diagnosed with subvalvular aortic stenosis. Cardiac catheterization revealed a left ventricular-aortic systolic pressure gradient of 90 mm Hg. Surgical correction of the condition was achieved using cardiopulmonary bypass. The subvalvular fibrous lesion was resected through an aortotomy. The stenosis was dilated from 8.5 mm to 12.0 mm in diameter. Postoperatively the dog was asymptomatic. Seven months after surgery, the pressure gradient decreased to 44 mm Hg. However, after another three months, the dog died suddenly without any premonitory signs. Postmortem examination revealed that pathologic changes caused by increased left ventricular pressure overload were not severe. 相似文献
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Minoru Ichihara Satoshi Uchida Sei Fujii Masayuki Yamashita Hitoshi Sawada Hidehiro Inagaki 《Weed Biology and Management》2014,14(2):99-105
This study aimed to determine whether seedling herbivory by the field cricket, Teleogryllus emma (Ohmachi et Matsuura), promoted the mortality of Italian ryegrass (Lolium multiflorum Lam.), a non‐native grass weed in Japan, whether the impact of seedling herbivory differed depending on the depth of seedling emergence and the influence of cricket density on seedling mortality. First, the seedlings at emergence depths of 0, 1 and 2 cm were exposed to the cricket for 2 days in plastic cups and the amount of seedling mortality by herbivory was calculated. The level of mortality of 60 seedlings that emerged from seeds on the soil surface was 92.4%, significantly higher than that of the seedlings that emerged from seeds at depths of 1 cm (10.8%) and 2 cm (9.6%). Second, the seedlings at the emergence depths of 0 and 1 cm were exposed to the crickets at four different densities (zero, one, two and three individuals per 5700 cm2) for 5 days in plastic containers and the amount of seedling mortality by herbivory was calculated. The level of mortality by herbivory of the seedlings that emerged from 100 seeds on the soil surface was higher (33.3–61.3%) than that of the seedlings that emerged from seeds at a depth of 1 cm (4.7–13.1%) in the containers with one‐to‐three crickets. The level of seedling mortality tended to increase with an increasing cricket density. These results suggest that seedling herbivory by crickets is an important factor in weed mortality, particularly in no‐till fields and in field margins, where the seeds are more prevalent near the soil surface. 相似文献