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The coagulase-negative staphylococcal species causing mastitis in lactating cattle were identified and possible differences in the clinical characteristics or persistence of mastitis caused by different CNS were evaluated. The effect of antimicrobial treatment was also assessed. In addition, AFLP-typing of CNS was compared with the phenotypic identification. A total of 133 clinical or subclinical quarter cases of intramammary infection caused by CNS from the practice area of the Ambulatory Clinic of the University of Helsinki were studied. Bacteriological diagnosis was based on biochemical (API) testing. Staphylococcus simulans (43.6%) followed by S. chromogenes (23.3%) were the most common CNS species isolated from the milk samples. Ninety-nine isolates were genotyped using AFLP-analysis. Only 75.0% of S. chromogenes and S. simulans isolates identified with API test were clustered with the type strains of these species. Approximately half of the mastitis cases were clinical, and in the majority clinical signs were mild. The severity and persistence of intramammary infection were unaffected by CNS species. Fifty-nine percent of the quarter cases were treated with antimicrobials, and the rest were left without treatment. Mastitis due to beta-lactamase-negative CNS was treated with penicillin G and that due to beta-lactamase-positive CNS with cloxacillin. Nineteen percent of the isolates were beta-lactamase-positive. The bacterial cure rate for quarters treated with antimicrobials was high, 85.9%, as opposed to only 45.5% for untreated quarters. Bacterial cure rates for the most common CNS species or AFLP clusters were not statistically different. Further studies on identification of CNS species are needed. 相似文献
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Janne Salminen Anne Hernesmaa Heli Karjalainen Hannu Fritze Martin Romantschuk 《Biology and Fertility of Soils》2010,46(2):113-126
Soil faunal activity stimulates terrestrial carbon and nutrient cycling and maintains soil physical structure. Interestingly, the effects of faunal activity on soil microbial community composition and activity is less clear, although the microbial community evidently is the key element of terrestrial decomposition processes and nutrient cycling. We manipulated the composition of soil fauna in mesh bags with various mesh sizes (5, 45, and 1,000 µm) to exclude faunal groups based on body size. Our experiment indicated that feeding and foraging activity of nematodes and enchytraeids modified the physical–chemical properties of the soil and had long-term effects on soil bacterial community composition (measured by denaturing gel electrophoresis, colony-forming unit counts, and phospholipid fatty acid pattern). However, microbial enzyme (phosphomonoesterase, butyrate-esterase, ß-glucosidase, N-acetyl-glucosamidase, and ß-cellobiosidase) activities (measured by fluorogenic model substrates), decomposition rate of woody and cellulose material, or C and N status of the soil were not altered by the faunal treatment. Instead, enzyme activity was reduced by soil drying. Our results give support to ideas that functional redundancy is a common characteristic of the soil organisms, but no support was found for the idea that the community composition of soil decomposers had a strong link to the regulation of C and N cycles in the soil. 相似文献
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不同水盐胁迫对番茄生长发育和产量的影响研究 总被引:1,自引:1,他引:0
【目的】探究番茄植株对不同水盐胁迫情景的响应,为合理制定盐碱化土壤下的灌溉制度提供科学依据。【方法】以粉欧宝番茄品种为研究对象,开展水盐对番茄生长发育影响的盆栽试验。试验采用完全随机布置,设置3个水分水平(W1-充分灌溉、W2-1/2的W1灌水量、W3-干旱复水)和2个盐分水平(S1-无盐和S2-0.3%含盐量),每个处理4个重复,测定了番茄耗水、干物质和产量指标,分析了不同水盐胁迫对番茄植株生长发育与产量的影响。【结果】与充分灌溉W1相比,W2水平的番茄植株耗水、干物质、植株含水率、叶质量、产量、单果质量显著减少。W3水平的植株耗水量和叶茎比显著减少,但单株干质量与鲜干比所受影响不大;单果鲜质量与干质量显著减小,但坐果率提高导致产量有所增加。盐分处理的番茄植株耗水量、单株干质量、鲜干比、叶茎比、果实总产量、单果鲜质量与干质量均小于无盐处理。水分胁迫显著影响叶片生长和单个果实发育,盐分胁迫抑制植株的生长发育及产量形成。【结论】干旱复水与无盐处理组合(W3S1)下番茄植株表现出了较好的生长发育状况和产量水平,可用于最优调亏灌溉制度的制定。 相似文献
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以"南杂二号"黄瓜为试材,利用遮光网对黄瓜幼苗连续寡照1、3、5h,均处理3、6、9、12d,研究了寡照条件下黄瓜叶片的光合特性;并利用非直角双曲线模型、指数模型、直角双曲线模型和直角双曲线修正模型4种模型模拟光响应曲线,选择最优的模型来模拟光合参数,以期为设施黄瓜种植光照优化控制提供科学依据。结果表明:直角双曲线修正模型最佳,其次为指数模型,非直角双曲线模型,直角双曲线模型,并利用最优的直角双曲线修正模型的模拟值计算出光合参数;寡照下净光合速率、最大光合速率、光饱和点、表观量子效率、气孔导度均降低,蒸腾速率先升高后降低,光补偿点、气孔限制值升高,水分利用率先减少后增加,连续寡照1、3、5h净光合速率较对照分别降低了23.56%、45.40%、62.60%。 相似文献
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Shi-xiang Zong You-qing Luo Ya-qin Cui Jian-wei Wang Wei Yan Ai-jie Liu Heliövaara Kari 《中国林学(英文版)》2009,11(1):24-27
The population density and distribution of the three major boring pests, Holcocerus artemisiae, Sphenoptera sp. and Adosopius sp. were studied in their host plant Artemisia ordosica. Results show that the larvae of H. artemisiae mainly bore the rhizome of A. ordosica, but also the larvae of Sphenoptera sp. and Adosopius sp. bore the rhizome of A. tordosica. The adults of Sphenoptera sp. and Adosopius sp. feed on the leaves of A. ordosica as a nutritional supplement. The distribution of the larvae of three pests in their host plant is completely different. H. artemisiae and Sphenoptera sp. are distributed in the whole A. ordosica plant. The newly hatched larvae first feed on the phloem and xylem of stem and then burrow to the roots before the winter
of the same year. However, Adosopius sp. is distributed mainly in the roots. The newly hatched larvae move from the phloem to the xylem, and gradually damage
the lower part of the trunk. The larvae of the three pests sometimes coexist in a single A. ordosica plant. However, the probability of the coexistence of the three pests is very small. The highest probability of coexistence
of two pests was observed in Sphenoptera sp. and H. artemisiae, while the probability of coexistence of Sphenoptera sp. and Adosopius sp. as well as H. artemisiae and Adosopius sp. was smaller. The reasons for coexistence or its avoidance are not only related to the amount of food provided nor the
volume of larval living space in a single A. ordosica plant, but also related to the development characteristics of different kinds of larvae and interspecific competition. 相似文献
19.
Juliane?HahnEmail authorView authors OrcID profile Heli?Juottonen Hannu?Fritze Eeva-Stiina?Tuittila 《Biology and Fertility of Soils》2018,54(4):533-547
Peatland restoration via rewetting aims to recover biological communities and biogeochemical processes typical to pristine peatlands. While rewetting promotes recovery of C accumulation favorable for climate mitigation, it also promotes methane (CH4) emissions. The potential for exceptionally high emissions after rewetting has been measured for Central European peatland sites previously grazed by cattle. We addressed the hypothesis that these exceptionally high CH4 emissions result from the previous land use. We analyzed the effects of cattle dung application to peat soils in a short- (2 weeks), a medium- (1 year) and a long-term (grazing) approach. We measured the CH4 production potentials, determined the numbers of methanogens by mcrA qPCR, and analyzed the methanogen community by mcrA T-RFLP-cloning-sequencing. Dung application significantly increased the CH4 production potential in the short- and the medium-term approach and non-significantly at the cattle-grazed site. The number of methanogens correlated with the CH4 production in the short- and the long-term approach. At all three time horizons, we found a shift in methanogen community due to dung application and a transfer of rumen methanogen sequences (Methanobrevibacter spp.) to the peatland soil that seemed related to increased CH4 production potential. Our findings indicate that cattle grazing of drained peatlands changes their methanogenic microbial community, may introduce rumen-associated methanogens and leads to increased CH4 production. Consequently, rewetting of previously cattle-grazed peatlands has the potential to lead to increased CH4 emissions. Careful consideration of land use history is crucial for successful climate mitigation with peatland rewetting. 相似文献
20.
Tero Heinonen Timo Pukkala Antti Asikainen Heli Peltola 《European Journal of Forest Research》2018,137(1):93-107
We used national scenario analyses to examine the effects of fertilization, use of improved regeneration material and ditch network maintenance (DNM), both separately and simultaneously, on timber production of Finnish forests under the current climate. We also analyzed how the area of artificial regeneration, forest fertilization, and DNM developed in different management and harvesting intensity scenarios. The initial data were obtained from the 11th National Forest Inventory of Finland, excluding protected forests. Four sets of even-flow harvesting scenarios with annual timber harvest targets of 60, 70, 80, and 90 million m3 were developed for 90-year simulation period. Use of improved material in artificial forest regeneration was assumed to result in 10% higher diameter and height increment compared to naturally regenerated seedlings. Sub-xeric pine-dominated and mesic spruce-dominated sites were fertilized, and 40% of drained peatlands were maintenance-ditched when they fulfilled a set of predetermined criteria for temperature sum, stand basal area, and mean tree diameter. As a result, when fertilization, improved regeneration material, and DNM were all used, the mean annual volume increment over the 90-year simulation period increased by 3.4–5.4 million m3 depending on harvesting intensity. The maximum sustainable harvest of timber would be almost 80 million m3 yr?1. The simulated fertilization area was about four times larger than the presently fertilized area, and the simulated DNM area was about the same as the current. Fertilization gave the largest additional 90-year volume increment and the DNM the smallest when they were used separately. The use of improved regeneration material gave the largest additional volume increment in southern Finland and fertilization in central and northern Finland. 相似文献