Microbial biomass and N mineralization in relation to N supply of sugar beet under reduced tillage

Reduced tillage may affect N supply of plants by influencing soil microbial biomass and thereby N release. The aim of this study was to evaluate changes in microbial biomass due to tillage in relation to N mineralization and to assess the contribution to the N supply of sugar beet. For this purpose, in a field trial near Göttingen in 1995 microbial biomass and net N mineralization were determined in an in situ incubation of ploughed and reduced tilled soil in plots which were not given application of mineral N fertilizer. In reduced tilled soil the increase in mineral N concentration in the upper 10 cm of soil was mainly attributed to an increase in microbial biomass. The organic matter was more easily decomposable, indicated by the increase in C_mic/C_org and N_mic/N_t ratios; this was further supported by the enhanced turnover of microbial biomass in reduced tillage plots. A regression function was used to relate seasonal fluctuations of microbial biomass, soil moisture and soil temperature to N mineralization rate. There was a good agreement between measured and calculated N mineralization rate. Reduced tillage affected N mineralization by affecting the quantity and quality of microbial biomass. In 0–30 cm soil depth 169 kg N/ha were mineralized, 30 kg more N than in ploughed soil. However, despite improved N availability, the N uptake of sugar beet was decreased in reduced tilled soil. Because the N concentration in plants did not differ, it was concluded that sugar beet growth in reduced tilled soil was impaired due to other factors than N supply.