干旱区绿洲农业结构与水资源合理利用研究——以新疆策勒绿洲为例

 绿洲农业是依靠灌溉发展起来的生态系统。干旱地区地表水资源共同特点是春、秋季供水紧张,夏季相对富裕;但种植业产业结构上用水又集中在春季,水的利用矛盾重重。本文分析新疆策勒绿洲近50年农业统计资料,结合课题组调查收集的第一手资料,在充分分析干旱区绿洲农业结构与水资源利用特点的基础上,用资源最优分配模型和线性优化模型方法,提出了绿洲农业结构、作物布局和水资源利用最佳方案,为干旱区绿洲水资源合理利用、区域经济发展提供科学依据。     

Impact of DNA damage on the frequency of sperm chromosomal aneuploidy in normal and subfertile men

Background: Various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. Moreover, sperm DNA damage is shown to be associated with male infertility. In this study, the rate of DNA damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. Methods: Semen samples were obtained from healthy normal and subfertile (oligozoospermia, asthenozoospermia, and oligoasthenozoospermia) men. The frequency of aneuploidy was assessed using primed in situ labeling (PRINS) analysis with specific primers for chromosomes 18, 21, X, and Y. Sperm DNA damage was assessed using alkaline comet assay. Results: The mean frequencies of disomy for the patients were significantly higher than normal for all chromosomes (P<0.01). The extent of DNA damage in sperms of subfertiles was significantly higher than in normal individuals (P<0.001). The obtained results indicated that higher rate of DNA damages led to higher frequency of chromosomal disomy except for asthenozoospermia samples which exhibited higher rate of DNA damage and lower frequency of chromosomal disomy. Conclusions: These results demonstrate that men with oligozoospermia and oligoasthenozoospermia have an elevated risk for chromosome abnormalities in their sperm, particularly sex chromosomes. DNA damage might be involved in the process of malsegregation of chromosomes.Asthenozoospermia, DNA damage, Primed in situ labeling (PRINS), Comet assay     

Effect of processing on the in vitro digestibility of proteins and carbohydrates in some Indian legumes

The effect of different types of processing such as boiling, pressure cooking, puffing, frying, germination, and germination followed by cooking on the protein and carbohydrate digestibility of chickpea, horsegram and cowpea were studied in in vitro systems. In the case of chickpea, the protein digestibility was not significantly improved by any of the treatments. However, for horsegram and cowpea, improvement in protein digestibility was observed after some of the different processing treatments. Frying decreased the protein digestibility in all of the pulses. All of the treatments, except germination, caused a marked increase in in vitro carbohydrate digestibility.     

Evaluation of ELISA in the serodiagnosis of bovine farcy

Mycobacterium farcinogenes is the causal agent of bovine farcy, a chronic infectious disease of zebu cattle in some parts of tropical Africa. Whole cell homologous antigen of M. farcinogenes was used in the standardization and evaluation of an enzyme linked immunosorbent assay (ELISA) for the detection of circulating antibodies against bovine farcy using sera from confirmed bovine farcy and from bovine farcy-free cattle. The cut-off optical density (OD) value was decided at 1.8 using filter 405nm after one hour of incubation at 37°C. Accordingly, 115 out of 124 (92.7%) serum samples from clinically proven bovine farcy cattle were reported sero-positive. Sera from cattle infected with M. avium and M. paratuberculosis revealed OD value <1.8, indicating the differential diagnostic ability of M. farcinogenes antigen. Our test sensitivity was 92.7% and specificity was 97%, therefore could be routinely employed to support early clinical diagnosis, epidemiological surveys and for screening animals before exportation to farcy-free regions.     

The prevalence of <Emphasis Type="Italic">Mycobacterium bovis</Emphasis>-infection and atypical mycobacterioses in cattle in and around Morogoro,Tanzania

A study was conducted to determine the prevalence of Mycobacterium bovis-infection and atypical mycobacterioses in different cattle herd management systems in and around Morogoro, Tanzania. Between April and June 2005, a total of 728 bovines from 49 herds were tested for M. bovis-infection and atypical mycobacterioses. Milk samples were taken from tuberculin positive animals and analysed for the presence of mycobacteria. Total prevalences of 2.5% and 10.1% were found for M. bovis-infection and atypical mycobacterioses respectively, with more M. bovis-infection in cattle in the extensive management system and more atypical mycobacterioses in cattle in the intensive management system. From 8 out of 42 milk samples (19%) atypical mycobacteria were cultured. A higher prevalence of M. bovis-infection in the extensive sector could be due to several factors. In addition, such high prevalence puts herd owners and their families at risk for BTB. Therefore control of BTB, as well as education of cattle owners is important, especially in the extensive sector.     

伊犁河谷盐渍化区土壤盐分离子的空间变异特征

利用地统计学,结合地理信息系统(GIS),在面积约为400 km2的伊犁河谷典型研究区总共布设了土壤取样点60个,测定土壤表层(0～20 cm)总盐分及其组成(Ca2+、Mg2+、K+、Na+、SO42-、Cl-、CO32-、HCO3-)的空间变异性规律.结果表明:总盐分,Ca2+,HCO3-为正态分布,其余盐分离子符...     

Immune responses of whiteleg shrimp,Litopenaeus vannamei (Boone, 1931), to bacterially expressed dsRNA specific to VP28 gene of white spot syndrome virus

In this study, dsRNA specific to VP28 gene of white spot syndrome virus (WSSV) of shrimp was synthesized in Escherichia coli in large scale and studied the immune response of shrimp to dsRNA‐VP28. The haematological parameters such as clotting time and total haemocytes counts, and immunological parameters such as prophenoloxidase (proPO), superoxide dismutase (SOD), superoxide anion (SOA) and malondialdehyde content, as well as the mRNA expression of ten immune‐related genes were examined to estimate the effect of dsRNA‐VP28 on the innate immunity of Litopenaeus vannamei. The activities of proPO, SOA and SOD significantly increased in haemocyte after dsRNA‐VP28 treatment, whereas MDA content did not change significantly. Among the ten immune‐related genes examined, only the mRNA expression of proPO, cMnSOD, haemocyanin, crustin, BGBP, lipopolysaccharides (LPs), lectin and lysozyme in haemocytes, gill and hepatopancreas of L. vannamei, was significantly upregulated at 12 h after dsRNA‐VP28 treatment, while no significant expression changes were observed in Toll receptor and tumour receptor genes. The increase of proPO and SOD activities, and SOA level and mRNA expression level of proPO, cMnSOD, haemocyanin, crustin, BGBP, LPs, lectin and lysozyme after dsRNA‐VP28 stimulation indicate that these immune‐related genes were involved in dsRNA‐VP28‐induced innate immunity in shrimp.     

Pan‐continental invasion of Pseudorasbora parva: towards a better understanding of freshwater fish invasions

In recent years, policy‐makers have sought the development of appropriate tools to prevent and manage introductions of invasive species. However, these tools are not well suited for introductions of non‐target species that are unknowingly released alongside intentionally‐introduced species. The most compelling example of such invasion is arguably the topmouth gudgeon Pseudorasbora parva, a small cyprinid species originating from East Asia. A combination of sociological, economical and biological factors has fuelled their rapid invasion since the 1960s; 32 countries (from Central Asia to North Africa) have been invaded in less than 50 years. Based on a combination of monitoring surveys (2535 populations sampled) and literature reviews, this paper aims to quantify and characterise important invasion parameters, such as pathways of introduction, time between introduction and detection, lag phase and plasticity of life history traits. Every decade, five new countries have reported P. parva introduction, mainly resulting from the movement of Chinese carps for fish farming. The mean detection period after first introduction was 4 years, a duration insufficient to prevent their pan‐continental invasion. High phenotypic plasticity in fitness related traits such as growth, early maturity, fecundity, reproductive behaviour and the ability to cope with novel pathogens has predisposed P. parva to being a strong invader. The Pseudorasbora parva invasion has provided quantitative data for the development of 1) early warning systems across different spatial scales; 2) rapid eradication programmes prior to natural spread in open systems and 3) sound risk assessments with emphasis on plasticity of life history traits.