Effects of Increasing Temperatures on Spikelet Fertility in Different Rice Cultivars based on Temperature Gradient Chamber Experiments

Spikelet sterility in rice (Oryza sativa L.) induced by high temperatures is a major concern given global warming predictions. We studied differences among eight rice cultivars in spikelet fertility at five different temperature levels in temperature gradient chamber (TGC) experiments. Six japonica and two indica cultivars were exposed to high‐temperature gradients in TGCs during the 2005 flowering season. Spikelet sterility increased with temperature in TGCs and differed among cultivars because of both variations in temperature tolerance and timing of heading. The correlation between spikelet fertility of individual panicles and both air temperature and panicle temperature during flowering was analyzed to compare tolerances among cultivars. The temperature (T₇₅) at which spikelet fertility was 75 % of maximum ranged from 34 to 39 °C air temperature and differed significantly among cultivars. Indica varieties had higher T₇₅ values than japonica varieties. The T₇₅ values based on panicle temperature also differed among cultivars, but the difference between indica and japonica varieties were less significant. We concluded that the higher temperature tolerances of indica cultivars in our experiments could be attributed to lower spikelet temperatures, and cultivars with similar spikelet temperatures still had different heat tolerances due to differences in pollination ability.     

An economic evaluation of Kanazawa and Shichika irrigation water’s multi-functional roles using CVM

In order to confirm that the regional function of Kanazawa’s irrigation water is representative of the urban type, and that Shichika’s irrigation water is representative of the rural, the contingent valuation method (CVM) was applied to determine the economic value of the functions aside from agricultural production, that is, the multi-functional roles of the irrigation water. The economic value of the regional function in Kanazawa was significantly lower than in the Shichika area, reflecting a difference in charge for irrigation water and people’s preference or consciousness in urban and rural areas. In urban areas, people tend to place a higher value on ground view, peace of mind and environmental friendliness. In contrast, in rural areas, irrigation water is presently used for domestic purposes. In other words, in urban areas people see water as public goods, while in rural areas the people see water as private goods. In addition, differences in the economic value of each item that was statistically significant for CVM were estimated using the Turnbull model comparing the items approved or rejected by the respondent.     

Complete replication of hepatitis C virus in cell culture

Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.     

Chemical interesterification of blends of palm stearin, coconut oil, and canola oil: physicochemical properties

trans-Free interesterified fat was produced for possible usage as a margarine. Palm stearin, coconut oil, and canola oil were used as substrates for chemical interesterification. The main aim of the present study was to evaluate the physicochemical properties of blends of palm stearin, coconut oil, and canola oil submitted to chemical interesterification using sodium methoxide as the catalyst. The original and interesterified blends were examined for fatty acid composition, softening and melting points, solid fat content, and consistency. Chemical interesterification reduced softening and melting points, consistency, and solid fat content. The interesterified fats showed desirable physicochemical properties for possible use as a margarine. Therefore, our result suggested that the interesterified fat without trans-fatty acids could be used as an alternative to partially hydrogenated fat.     

Analysis of water balance in the Tedori river alluvial fan areas of Japan: focused on quantitative analysis of groundwater recharge from river and ground surface, especially paddy fields

Water balance in the Tedori River alluvial fan areas was analyzed for all components of the hydrological cycle based on exchange of the channel/soil surface and aquifer horizon fractions with river water. The results were summarized on an annual basis, as well as for the irrigation and non-irrigation periods. The study area received 6.28 mm/day of precipitation and had an outflow of 2.32 mm/day as direct runoff, resulting in 3.96 mm/day of water being supplied to the soil surface. The channel/soil horizon fraction received this 3.96 mm/day, as well as 9.12 mm/day intake water from the head works. Conversely, 2.74 and 2.85 mm/day were lost by evapotranspiration and percolation, respectively. Thus, surface runoff of 7.49 mm/day flowed from the study area to the Sea of Japan or drainage canals near the river mouth. In the aquifer horizon fraction, 2.85 mm/day of water was supplied from the channel/soil horizon fraction and 2.15 mm/day was supplied from the Tedori River, while 1.73 mm/day was extracted by groundwater. Thus, 3.27 mm/day of groundwater flowed out to the Sea of Japan or into downstream drainage canals. An outline of the water balance of the irrigation and non-irrigation period is also shown. Because various hydrological components are closely related to each other, planning and management of water resources for individual goals are not adequate, but require the integrated aspect of water balance for sustainable water use.     

Cat-scratch disease in veterinary-associated populations and in its cat reservoir in Taiwan

In Taiwan, the first human case of cat-scratch disease (CSD) was diagnosed by a serologic test in 1998. Since then, no studies have been conducted to understand the epidemiology of the infection in Taiwan. Therefore, this study is the first epidemiologic survey of CSD in cats and humans in this country. Using veterinary-associated individuals as the study population, it was identified that 1.7% of them were seropositive for B. henselae, and residence was the only factor associated with seropositivity. Bartonella species were successfully isolated from 25 (19.1%) of the 131 cats tested. Only B. henselae and B. clarridgeiae were obtained from bacteremic cats. Furthermore, 9.2% of 131 cats were dually-infected with genotypes I and II of B. henselae. It is the highest prevalence of co-infection that has ever been reported worldwide. In cats, the seroprevalence was 23.7% by indirect immunofluorescence antibody assay with B. henselae Houston-1 (type I) as the antigen. When 12 bacteremic but seronegative cats were re-tested by IFA slides coated with B. henselae U-4 antigen (type II), 9 cats were identified to be seropositive. Our study further suggested that using only direct PCR of 16S-23S rRNA intergenic region or the combination of the PCR method and indirect immuno-fluorescence test will be useful to diagnose Bartonella-free cats.     

Fucoxanthin and fucoxanthinol enhance the amount of docosahexaenoic acid in the liver of KKAy obese/diabetic mice

This study examined the effect of dietary fucoxanthin or fucoxanthinol on the amount of docosahexaenoic acid (DHA) in the liver of KKAy mice, a model for obese/type II diabetes. In the first experiment, mice were fed diets containing crude fucoxanthin or glyceroglycolipid for 4 weeks. Results showed a significant increase in the level of DHA in mice fed 0.53% crude fucoxanthin, from 2.3% in control mice to 5.1% of fatty acid composition of total liver lipids. On the other hand, in mice fed crude glyceroglycolipid, the level of DHA as a proportion of total liver fatty acids remained unchanged. To clarify the enhancement of hepatic DHA, in the second experiment, KKAy mice were fed a diet containing purified fucoxanthin or its deacetylated derivative, fucoxanthinol. Results from a quantitative analysis using an internal standard showed that in mice fed 0.2% fucoxanthin, the amount of hepatic DHA was 2-fold higher than in control mice, whereas DHA levels in the small intestine remained unchanged. Furthermore, 0.2% fucoxanthinol led to 1.8- and 1.2-fold increases in the amount of hepatic DHA and arachidonic acid compared to control mice, respectively. These results indicate for the first time that dietary fucoxanthin and fucoxanthinol enhance the amount of DHA in the liver of KKAy mice.