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51.
Wolfgang Schaaf Oliver Bens Anton Fischer Horst H. Gerke Werner Gerwin Uwe Grünewald Hartmut M. Holländer Ingrid Kögel‐Knabner Michael Mutz Michael Schloter Rainer Schulin Maik Veste Susanne Winter Reinhard F. Hüttl 《植物养料与土壤学杂志》2011,174(2):229-239
Ecosystems are characterized as complex systems with abiotic and biotic processes interacting between the various components that have evolved over long‐term periods. Most ecosystem studies so far have been carried out in mature systems. Only limited knowledge exists on the very initial phase of ecosystem development. Concepts on the development of ecosystems are often based on assumptions and extrapolations with respect to structure–process interactions in the initial stage. To characterize the effect of this initial phase on structure and functioning of ecosystems in later stages, it is necessary to disentangle the close interaction of spatial and temporal patterns of ecosystem structural assemblages with processes of ecosystem development. The study of initial, less complex systems could help to better identify and characterize coupled patterns and processes. This paper gives an overview of concepts for the initial development of different ecosystem compartments and identifies open questions and research gaps. The artificial catchment site “Chicken Creek” is introduced as a new research approach to investigate these patterns and processes of initial ecosystem development under defined boundary conditions. This approach allows to integrate the relevant processes with related pattern and structure development over temporal and spatial scales and to derive thresholds and stages in state and functioning of ecosystems at the catchment level. 相似文献
52.
Linden H 《Science (New York, N.Y.)》2002,297(5582):777-778
53.
Vogeley L Sineshchekov OA Trivedi VD Sasaki J Spudich JL Luecke H 《Science (New York, N.Y.)》2004,306(5700):1390-1393
Microbial sensory rhodopsins are a family of membrane-embedded photoreceptors in prokaryotic and eukaryotic organisms. Structures of archaeal rhodopsins, which function as light-driven ion pumps or photosensors, have been reported. We present the structure of a eubacterial rhodopsin, which differs from those of previously characterized archaeal rhodopsins in its chromophore and cytoplasmic-side portions. Anabaena sensory rhodopsin exhibits light-induced interconversion between stable 13-cis and all-trans states of the retinylidene protein. The ratio of its cis and trans chromophore forms depends on the wavelength of illumination, thus providing a mechanism for a single protein to signal the color of light, for example, to regulate color-sensitive processes such as chromatic adaptation in photosynthesis. Its cytoplasmic half channel, highly hydrophobic in the archaeal rhodopsins, contains numerous hydrophilic residues networked by water molecules, providing a connection from the photoactive site to the cytoplasmic surface believed to interact with the receptor's soluble 14-kilodalton transducer. 相似文献
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56.
Mertens-Talcott SU Rios J Jilma-Stohlawetz P Pacheco-Palencia LA Meibohm B Talcott ST Derendorf H 《Journal of agricultural and food chemistry》2008,56(17):7796-7802
The acai berry is the fruit of the acai palm and is traditionally consumed in Brazil but has gained popularity abroad as a food and functional ingredient, yet little information exists on its health effect in humans. This study was performed as an acute four-way crossover clinical trial with acai pulp and clarified acai juice compared to applesauce and a non-antioxidant beverage as controls. Healthy volunteers (12) were dosed at 7 mL/kg of body weight after a washout phase and overnight fast, and plasma was repeatedly sampled over 12 h and urine over 24 h after consumption. Noncompartmental pharmacokinetic analysis of total anthocyanins quantified as cyanidin-3-O-glucoside showed Cmax values of 2321 and 1138 ng/L at t max times of 2.2 and 2.0 h, and AUC last values of 8568 and 3314 ng h L(-1) for pulp and juice, respectively. Nonlinear mixed effect modeling identified dose volume as a significant predictor of relative oral bioavailability in a negative nonlinear relationship for acai pulp and juice. Plasma antioxidant capacity was significantly increased by the acai pulp and applesauce. Individual increases in plasma antioxidant capacity of up to 2.3- and 3-fold for acai juice and pulp, respectively were observed. The antioxidant capacity in urine, generation of reactive oxygen species, and uric acid concentrations in plasma were not significantly altered by the treatments. Results demonstrate the absorption and antioxidant effects of anthocyanins in acai in plasma in an acute human consumption trial. 相似文献
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Five strains of Clostridium septicum were used to prepare bacterins, bacterin-toxoids, toxoid, and combinations of bacterins or bacterin-toxoids. These preparations were tested for immunogenicity in guinea pigs vaccinated subcutaneously with 1.0 ml of product. Usually, a second vaccination was given 21 to 24 days later. The immunity of groups of vaccinated guinea pigs was challenged with as many as 22 strains of C septicum. When challenge exposed with homologous strains at 21 to 24 days after one vaccination or 10 t0 18 days after a second vaccination, 60% to 100% of the guinea pigs in each group survived. Demonstrable cross-protection among strains of C septicum varied from none to 100% protection in vaccinated guinea pigs. A combination of bacterin-toxoid prepared from four selected strains protected 70% to 100% of the vaccinated guinea pigs challenge exposed with 21 strains. Duration-of-immunity studies demonstrated a twofold to fourfold decrease in protection when the vaccination-to-challenge interval was extended an additional 3 weeks. Strains of C septicum do not have an effective common immunogen and the stimulated immunity appears to be of short duration. Antitoxin was demonstrated to be less important than other factors in protecting against C septicum infection. 相似文献
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Julia Janke Martina Bandte Christian Ulrichs Giselher Grabenweger Barbara Jäckel Hartmut Balder Carmen Büttner 《Journal of pest science》2009,82(1):47-53
The parasitoid wasp Pnigalio agraules (Wlk.) is a key natural enemy of the horsechestnut leafminer Cameraria ohridella Deschka and Dimić (Lepidoptera: Gracillariidae). As a basis for mark-release-recapture studies, aimed at investigating the
dispersal of this parasitoid in the field, adults of P. agraules were marked using a vertebrate-specific immunoglobulin (IgG). The marker was later detected by double antibody sandwich enzyme-linked
immunosorbent assay (DAS-ELISA). The IgG was either applied externally by spraying or internally by feeding an IgG-enriched
diet. Different concentrations of the marker were used and the influence of abiotic (climatic conditions, time elapsed between
marking and marker examination) and biotic factors (sex and age of the parasitoids) on the detection of the immunomarker was
tested. External marking by spraying led to more homogeneous labelling than feeding the marker. Parasitoids labelled with
0.25 mg rabbit IgG per ten individuals contained enough immunomarker to be easily distinguished from unmarked ones. Neither
the climatic conditions nor the sex or age of the insects had an influence on the detection of the marker. The IgG remained
well detectable during the entire lifespan of the parasitoids, which was not negatively affected by the marking procedure.
Serological marking can be used to investigate the dispersal behaviour of beneficial insects within mark-release-recapture
studies. 相似文献