QTL detection for flowering time in faba bean and the responses to ambient temperature and photoperiod

Faba bean (Vicia faba L.) is a grain legume primarily used for animal feed and human food grown in a range of environments, globally. Time of flowering in faba bean is critical for adaptation to specific environments and is controlled largely by factors such as ambient temperature and photoperiod. The aim of this study was to investigate the genetic control of flowering time and the responses of flowering time to ambient temperature and photoperiod in faba bean. A bi-parental recombinant inbred line population (Icarus × Ascot) was evaluated over three years in field trials and three different controlled environments with varying temperatures and photoperiods. QTL analysis identified eight regions of co-localised QTLs associated with days to flowering, thermal time to flowering and node of first flower; on Chr-I.A/III/V, Chr-I.B.3, Chr-III.1, Chr-III.2, Chr-V.1 and Chr-V.2. Two of the detected regions are common with previously detected QTLs, up to two more are possibly common and the remaining four appear to be novel. For the first time, the associations of these QTLs with ambient temperature and photoperiod response were described. Candidate genes for some of the QTLs were identified using the associations with ambient temperature and photoperiod response together with knowledge extended from other legumes that have a syntenic relationship with faba bean.     

Heterosis and combining ability vis-à-vis association for green fruit yield and component traits involving male sterile lines in chilli (Capsicum annuum) under wet temperate zone of North Western Himalayas

Fourteen genetically diverse inbred lines comprising of four genic male sterile lines and 10 often pollinated lines as testers were crossed following ‘line × tester’ mating design to generate 40 F₁s. These F₁s, parents and check ‘CH-27’ were evaluated in α-lattice square design, replicated thrice during summer seasons of 2020 and 2021. The male sterile line DPChMS 9-2 was good general combiner for marketable fruit yield and eight other component traits while testers namely, DPCh 10, PBC 535, VVG, Him Palam Mirch-2 and DPCh 40 showed significant GCA for majority of yield related traits. The hybrids DPChMS 9-2 × HPM-2, DPChMS 9-2 × DPCh 40, DPChMS 9-2 × DPCh 101, DPChMS 9-2× VVG and DPChMS 26-1× HPM-1 were the most desirable based on specific combining ability (SCA) and economic heterosis for fruit yield and other important traits. Heterosis was positively correlated with SCA indicating prediction of potential hybrids can be made based on both these parameters simultaneously. Present study provides an opportunity to exploit top hybrids for stability and commercial cultivation.     

Evaluation of Cellulolytic Enzyme-Assisted Microwave Extraction of Punica granatum Peel Phenolics and Antioxidant Activity

Plant Foods for Human Nutrition - Conventional techniques for phenolics extraction from pomegranate (Punica granatum) peel (PP) have several insufficiencies like longer time duration, bioactive...     

Carbon storage and nitrogen cycling in silvopastoral systems on a sodic in northwestern India

Tree-based land-use systems could sequester carbon in soil and vegetation and improve nutrient cycling within the systems. The present investigation was aimed at analyzing the role of tree and grass species on biomass productivity, carbon sequestration and nitrogen cycling in silvopastoral systems in a highly sodic soil. The silvopastoral systems (located at Saraswati Reserved Forest, Kurukshetra, 29°4prime; to 30°15prime; N and 75°15prime; to 77°16prime; E) consisted of about six-year-old-tree species of Acacia nilotica, Dalbergia sissoo and Prosopis juliflora in the mainplots of a split-plot experiment with two species of grasses, Desmostachya bipinnata and Sporobolus marginatus, in the subplots. The total carbon storage in the trees + grass systems was 1.18 to 18.55 Mg C ha⁻¹ and carbon input in net primary production varied between 0.98 to 6.50 Mg C ha⁻¹ yr⁻¹. Carbon flux in net primary productivity increased significantly due to integration of Prosopis and Dalbergia with grasses. Compared to 'grass-only' systems, soil organic matter, biological productivity and carbon storage were greater in the silvopastoral systems. Of the total nitrogen uptake by the plants, 4 to 21 per cent was retained in the perennial tree components. Nitrogen cycling in the soil-plant system was found to be efficient. Thus, It is suggested that the silvopastoral systems, integrating trees and grasses hold promise as a strategy for improving highly sodic soils. This revised version was published online in June 2006 with corrections to the Cover Date.     

Bioamelioration of a sodic soil by silvopastoral systems in northwestern India

In sodic soils, excessive amounts of salts have an adverse effect on soil biological activity and stability of soil organic matter. The study analyzes the role of silvopastoral systems to improve soil organic matter and microbial activity with a view for effective management of soil fertility. The silvopastoral systems for the present study (located at Saraswati Reserved Forest, Kurukshetra; 29°4′ to 30°15′ N and 75°15′ to 77°16prime; E) are characterized by tree species of Acacia nilotica, Dalbergia sissoo and Prosopis juliflora along with grass species of Desmostachya bipinnata and Sporobolus marginatus. Soil microbial biomass carbon was measured using the fumigation extraction technique and nitrogen mineralization rates using aerobic incubation method. The microbial biomass carbon in the soils of D. bipinnata and S. marginatus treatments were low. In silvopastoral systems, microbial biomass carbon increased due to increase in the carbon content in the soil – plant system. A significant relationship was found between microbial biomass carbon and plant biomass carbon (r = 0.83) as well as the flux of carbon in net primary productivity (r = 0.92). Nitrogen mineralization rates were found greater in silvopastoral systems compared to 'grass-only' system. Soil organic matter was linearly related to microbial biomass carbon, soil N and nitrogen mineralization rates (r = 0.95 to 0.98, p < 0.01). On the basis of improvement in soil organic matter, enlarged soil microbial biomass pool and greater soil N availability in the tree + grass systems, agroforestry could be adopted for improving the fertility of highly sodic soil. This revised version was published online in June 2006 with corrections to the Cover Date.     

Seed Priming Increases Crop Yield Possibly by Modulating Enzymes of Sucrose Metabolism in Chickpea

The number of seeds and seed yield per plant were higher in chickpea crops raised from water and mannitol (4 %) primed seeds in comparison with the control non-primed crops. In primed plants, an enhanced acid invertase activity in the apical part of the main stem and the part immediately below it at 100 and 130 days after sowing (DAS) might result in an increased availability of hexoses to these plant parts. In addition, decreased acid invertase activity at the point of initiation of branches and in the internodes of stem observed in primed plants indicated restricted hydrolysis of sucrose during its transport through the stem, resulting in its more supply to the actively growing sinks. The activities of sucrose-cleaving enzymes, i.e. invertase and sucrose synthase (SS) in podwall of primed plants were higher at 110 DAS. At 140 DAS, a stage of rapid seed filling, increased activities of SS and sucrose phosphate synthase (SPS) were observed in seeds of primed plants. Increased SPS activity in seeds of primed crop could meet the increased assimilate requirements of the developing seeds. Higher activity of SS in seeds of primed crop may facilitate seed filling. These data suggest that enzymes of sucrose metabolism play an important role in increasing the yield of chickpea crops raised from primed seeds.     

Bioactivity of pyrogallol against melon fruit fly, <Emphasis Type="Italic">Bactrocera cucurbitae</Emphasis>

The insecticidal effects of pyrogallol were studied by treating eggs and larvae of the melon fruit fly, Bactrocera cucurbitae (Coquillett) (Tephritidae: Diptera), with various concentrations (1, 5, 25, 125, 625 and 3125 ppm) of the phenolic compound. Although egg hatching decreased following treatment of 0–8-h old eggs with pyrogallol, the decrease was not significantly different from the control. Larval period and total development period declined significantly in 64–72-h-old and 88–96-h-old B. cucurbitae larvae fed on pyrogallol-treated diet. However, in the 44–48-h-old larvae, the larval period and total development period were not affected by pyrogallol treatment at any of the tested concentrations. None of them survived up to the pupal stage at the highest concentration. Number of pupae formed and adult emergence decreased significantly in all larval instars following feeding on pyrogallol-treated diet. The analysis of enzymes in 64–72-h-old larvae treated with LC₄₀ concentration (16.21 ppm) of pyrogallol at three time intervals, i.e., 24 h, 48 h and 72 h, showed significant induction in the activities of ascorbate peroxidase (APOX) and glutathione S-transferases (GSTs) at 24 h but a decrease was observed following prolonged treatment. On the other hand, superoxide dismutase (SOD) and peroxidases (POX) activity remained suppressed during the initial treatment interval but increased with prolonged treatment in 136–144-h-old larvae. The catalase (CAT) activity was suppressed at all treatment durations whereas glutathione reductase (GR) activity was not affected by pyrogallol treatment. An increase in the activities of ascorbate peroxidase, superoxide dismutase, peroxidases and glutathione S-transferases indicates an induction of defensive response of the melon fruit fly to the toxic effects produced by ingestion of pyrogallol. Although the effects of the compound on enzyme activity were tested on second instar, it would be interesting to see the effects on other instars too.     

Pre-inoculation with Hyaloperonospora parasitica reduces incubation period and increases severity of disease caused by Albugo candida in a Brassica juncea variety resistant to downy mildew

Studies were undertaken to determine any interaction of an asymptomatic isolate of Hyaloperonospora parasitica (downy mildew) with a virulent isolate of Albugo candida (white rust) and Brassica juncea. White rust symptoms appeared 4 days earlier and were significantly more severe when a variety of B. juncea resistant to downy mildew but highly susceptible to white rust was first inoculated with A. candida followed 10 days later with H. parasitica. DNA extractions of tissues indicated H. parasitica had colonized the asymptomatic plants systemically. These are the first reports of (a) the systemic colonization by H. parasitica in a host resistant to downy mildew, and (b) the increase in susceptibility of a Brassica to white rust disease from asymptomatic colonization by H. parasitica.     

Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India

Johne's disease is chronic granulomatous infectious enteritis of animals caused by Mycobacterium avium subspecies paratuberculosis. A total of 153 animals from 19 dairy farms, 2 gaushalas (unproductive-animal rehabilitation centers), 2 goat and 2 sheep farms from different districts of the Punjab region were selected on the basis of clinical signs of disease. All samples from cattle (n = 86), buffalo (n = 34), goat (n = 25) and sheep (n = 26) were subjected to Ziehl-Neelsen staining and DNA extraction by a freeze and thaw method. Ziehl-Neelsen staining detected 71% samples positive for acid-fast bacilli whereas IS900 PCR detected 55% positive for Map DNA. IS1311 PCR-REA analysis of IS900 positive samples revealed ‘Bison’ type as the most prevalent (82%) genotype of Map, infecting all domestic ruminants. ‘Cattle’ type was present in a minority of cases (15%) from cattle, buffaloes and goats. This is the first report of ‘Cattle’ type Map from buffalo and goat species in India.