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501.
Rune H. Økland Harald Bratli Wenche E. Dramstad Anette Edvardsen Gunnar Engan Wendy Fjellstad Einar Heegaard Oddvar Pedersen Heidi Solstad 《Landscape Ecology》2006,21(7):969-987
Knowledge of variation in vascular plant species richness and species composition in modern agricultural landscapes is important
for appropriate biodiversity management. From species lists for 2201 land-type patches in 16 1-km2 plots five data sets differing in sampling-unit size from patch to plot were prepared. Variation in each data set was partitioned
into seven sources: patch geometry, patch type, geographic location, plot affiliation, habitat diversity, ecological factors,
and land-use intensity. Patch species richness was highly predictable (75% of variance explained) by patch area, within-patch
heterogeneity and patch type. Plot species richness was, however, not predictable by any explanatory variable, most likely
because all studied landscapes contained all main patch types – ploughed land, woodland, grassland and other open land – and
hence had a large core of common species. Patch species composition was explained by variation along major environmental complex
gradients but appeared nested to lower degrees in modern than in traditional agricultural landscapes because species-poor
parts of the landscape do not contain well-defined subsets of the species pool of species-rich parts. Variation in species
composition was scale dependent because the relative importance of specific complex gradients changed with increasing sampling-unit
size, and because the amount of randomness in data sets decreased with increasing sampling-unit size. Our results indicate
that broad landscape structural changes will have consequences for landscape-scale species richness that are hard or impossible
to predict by simple surrogate variables. 相似文献
502.
Schmid B Ribbe MW Einsle O Yoshida M Thomas LM Dean DR Rees DC Burgess BK 《Science (New York, N.Y.)》2002,296(5566):352-356
One of the most complex biosynthetic processes in metallobiochemistry is the assembly of nitrogenase, the key enzyme in biological nitrogen fixation. We describe here the crystal structure of an iron-molybdenum cofactor-deficient form of the nitrogenase MoFe protein, into which the cofactor is inserted in the final step of MoFe protein assembly. The MoFe protein folds as a heterotetramer containing two copies each of the homologous alpha and beta subunits. In this structure, one of the three alpha subunit domains exhibits a substantially changed conformation, whereas the rest of the protein remains essentially unchanged. A predominantly positively charged funnel is revealed; this funnel is of sufficient size to accommodate insertion of the negatively charged cofactor. 相似文献
503.
Karin Taus Friedrich Schmoll Ziad El‐Khatib Herbert Auer Heidemarie Holzmann Stephan Aberle Shiva Pekard‐Amenitsch Stefanie Monschein Tatjana Sattler Romana Steinparzer Franz Allerberger Daniela Schmid 《Zoonoses and public health》2019,66(7):842-851
We investigated the prevalence of Hepatitis E Virus (HEV), Leptospira and Ascaris suum (A. suum) seropositivity, and of nasal methicillin‐resistant Staphylococcus aureus (MRSA) colonization among Austrian practising veterinarians, and assessed the association with occupational swine livestock exposure. The 261 participants completed a questionnaire on demographics, intensity of occupational swine livestock contact and glove use during handling animals and their secretions. Participants' blood samples were tested for HEV, Leptospira and A. suum seropositivity and nasal swabs cultured for MRSA. We compared swine veterinarians (defined as >3 swine livestock visits/week) to non‐swine veterinarians (≤3 swine livestock visits/week) with regard to the outcomes through calculating prevalence ratio (PR) and 95% confidence interval (CI). Furthermore, the relationship between occupational swine livestock contact and the study outcomes was examined by age (</≥55 years) and glove usage. The prevalence of nasal MRSA colonization was 13.4% (95% CI: 9.3–17.6), of HEV seropositivity 20.8% (95% CI: 15.8–25.7) and A. suum seropositivity 44% (95% CI: 37.7–50.2). The highest anti‐leptospiral antibodies titres were 1:200 (L. hebdomadis) and 1:100 (L. autumnalis, L. caicola) found in three non‐swine veterinarians. Compared to non‐swine veterinarians, swine veterinarians were 1.9 (95% CI: 1.0–3.4) and 1.5 (95%CI: 1.0–2.3) times more likely HEV seropositive and A. suum seropositive, respectively, and 4.8 (95%CI: 2.5; 9.3) times more likely nasally colonized with MRSA. Among glove‐using veterinarians, occupational swine contact was no longer a determinant for HEV seropositivity (PR 1.6; 95% CI: 0.8–2.9). Similar was found for A. suum seropositivity, which was no longer associated with occupational swine livestock contact in the subgroup of glove using, ≥55‐year‐old veterinarians (PR: 1.07; 95% CI: 0.4–3.3). Our findings indicate that >3 occupational swine livestock visits per week is associated with HEV and A. suum seropositivity and nasal MRSA colonization and that glove use may play a putative preventive role in acquiring HEV and A. suum. Further analytical epidemiological studies have to prove the causality of these associations. 相似文献
504.
Jelena Kolarevic Roger Selset Olga Felip Christopher Good Kevin Snekvik Harald Takle Elisabeth Ytteborg Grete Bæverfjord Torbjørn Åsgård Bendik Fyhn Terjesen 《Aquaculture Research》2013,44(11):1649-1664
The objective of this study was to determine the long‐term effects of ambient unionized ammonia nitrogen (NH3‐N) combined with different feeding regimes on Atlantic salmon Salmo salar L parr growth, welfare and smoltification. Previous studies on the parr stage of Atlantic salmon have mostly focused on acute exposure, or at low temperatures. Atlantic salmon parr were exposed for 105 days (at 12°C, pH 6.8) to four sublethal ammonia concentrations ranging from 0.1 to 35 μg L?1 NH3‐N (0.1–25 mg L?1 TAN) at two feeding levels: full feed strength (+20% overfeeding) and 1/3 of full feed strength. After 21 days, it was observed that 32 μg L?1 NH3‐N reduced growth rate of parr fed full ration, but this effect was not evident at the end of the exposure. Feed utilization was not affected by ammonia exposure at any sampling point. Increasing ammonia levels were associated with a higher prevalence and severity of gill damage at 22 days but not at the end of the exposure. The examination of welfare indicators revealed only a few pathologies, not related to ammonia exposure. In addition, higher ammonia concentrations did not appear to influence the development of hypo‐osmoregulatory ability during parr‐smolt transformation. 相似文献
505.
Klopčič Matija Mina Marco Bugmann Harald Bončina Andrej 《European Journal of Forest Research》2017,136(5-6):1071-1090
European Journal of Forest Research - In the Dinaric Mountains, the future of silver fir and Norway spruce appears to be uncertain, especially given the threat of climate change to both species and... 相似文献
506.
A.L.M. Oliveira M. Stoffels M. Schmid V.M. Reis J.I. Baldani A. Hartmann 《European Journal of Soil Biology》2009,45(1):106-113
Micropropagated sugarcane plants have been used in Brazil for almost three decades. Besides the improvement in plant health, micropropagated sugarcane carries no endophytic plant growth-promoting bacteria. The Brazilian inoculation technology to reintroduce diazotrophic bacteria in micropropagated sugarcane plantlets revealed a synergistic-like effect in PGP-bacteria mixed inoculations. The infection model of single diazotrophic bacteria species in sugarcane was studied in detail, but still many questions remain open. In this study we used a combined fluorescence in situ hybridization (FISH) and a cultivation based approach (MPN) to evaluate the colonization of sugarcane plantlets by mixed inocula. The highest colonization for three out of the five species studied was obtained with a mixed inoculum, when the Azospirillum amazonense showed an increase by almost 100 times in colonization and Herbaspirillum spp. and Burkholderia tropica was determined at 107 cells per gram root fresh weight. All of the inoculated bacterial species could be detected using the FISH probes 12 h after bacterial inoculation. The FISH results confirmed the MPN counts and showed differences in the population numbers and colonization behavior of particular bacterial inoculum strains in the different mixed inocula. A putative antagonistic effect among the inoculated H. seropedicae and H. rubrisubalbicans strains was observed using FISH, as well as the better competitiveness of B. tropica as compared to the A. amazonense strain. The observed data probably reflect also specific interactions with the sugarcane variety used in this particular inoculation system, and may not be generalized as a rule. This is the first study about the competition for sugarcane colonization in a mixed bacterial inoculum. 相似文献
507.
H?vard Bj?rgen ?ystein Wessel Per Gunnar Fjelldal Tom Hansen Harald Sveier H?kon Rydland S?b? Katrine Bones Enger Eirik Monsen Agnar Kvellestad Espen Rimstad Erling Olaf Koppang 《Veterinary research》2015,46(1)
Melanised focal changes (black spots) are common findings in the white skeletal muscle of seawater-farmed Atlantic salmon (Salmo salar). Fillets with melanised focal changes are considered as lower quality and cause large economic losses. It has been suggested that red focal changes (red spots) precede the melanised focal changes. In the present work, we examined different populations of captive and wild salmon for the occurrence of both types of changes, which were investigated for the presence of different viruses by immunohistochemistry and RT-qPCR. The occurrence of red or melanised foci varied significantly between the populations, from none in wild fish control group, low prevalence of small foci in fish kept in in-house tanks, to high prevalence of large foci in farm-raised salmon. Large amounts of Piscine orthoreovirus (PRV) antigen were detected in all foci. No other viruses were detected. Red focal changes contained significantly higher levels of PRV RNA than apparently non-affected areas in white muscle of the same individuals. Some changes displayed a transient form between a red and melanised pathotype, indicating a progression from an acute to a chronic manifestation. We conclude that PRV is associated with the focal pathological changes in the white muscle of farmed Atlantic salmon and is a premise for the development of focal melanised changes. 相似文献
508.
IMAGING DIAGNOSIS—RADIOGRAPHY AND COMPUTED TOMOGRAPHY OF RADIOULNAR ISCHEMIC NECROSIS IN A JACK RUSSELL TERRIER 下载免费PDF全文
Lisbeth Schmid Andrea Klang Nikola Katic Agustina Ansón Michaela Gumpenberger Sibylle Kneissl 《Veterinary radiology & ultrasound》2018,59(1):E7-E11
A 7‐year‐old Jack Russell Terrier with a history of minor trauma was presented for lameness of the left forelimb. Radiography and computed tomography demonstrated a localized radioulnar osteolytic lesion with cortical bone loss and enthesiophytes. Based on results of diagnostic imaging and histopathological examination, the final diagnosis was radioulnar ischemic necrosis (RUIN), complicated by pathologic fracture. A rare disorder of unknown etiology, RUIN may be secondary to tearing of the interosseous ligament and potential ischemia. It should be differentiated from neoplastic or fungal disease. To the authors´ knowledge, this is the first canine case report describing RUIN. 相似文献