西咪替丁在体内对地塞米松预处理鸡免疫状态的影响

对地塞米松预处理鸡投以１０－１００ｍｇ／ｋｇ剂量的西咪替丁,进行了后者对前者作用影响的观察。结果表明：地塞米松能抑制伴刀豆球蛋白Ａ（ｃｏｎＡ）引起的鸡外周血淋巴细胞（ＢＰＬ）之ＩＬ－２活性,而西咪替丁对这种抑制作用有显著的拮抗作用;地塞米松能抑制动物对绵工细胞（ＳＲＢＣ）和牛血清白蛋白（ＢＳＡ）等２种胸腺依赖性抗原和非胸腺依赖性抗原流产布鲁氏菌（ＢＡ）的抗体应答,但这种抑制作用会因西咪替丁逆转;西     

菠萝果肉酸奶配方筛选及优化

研究以鲜牛奶、菠萝果肉为主要原料,通过正交实验,确定菠萝果肉酸奶生产最佳的工艺条件.结果表明:添加糖4%,菠萝果肉3%,琼脂0.05%,44 ℃条件下发酵3 h,获得的菠萝果肉酸奶品质最佳.另外,产品融合了酸奶和菠萝的风味,组织状态均匀,口感好,丰富了酸奶的品种.     

Mycoplasma pneumoniae induces IL-1β production through activating NLRP3 inflammasome by ROS in RAW264.7 cells

AIM: To investigate whether Mycoplasma pneumoniae (Mp)-induced interleukin-1β (IL-1β) production in RAW264.7 cells is through the activation of NLRP3 inflammasome via reactive oxygen species (ROS). ME-THODS: RAW264.7 cells were randomly divided into 3 groups. In normal group, RAW264.7 cells were treated without Mp. In model group, RAW264.7 cells were treated with 1∶ 10 multiplicity of infection (MOI) of Mp. In NAC group, RAW264.7 cells were pretreated with N- acetylcysteine (NAC) at a concentration of 5 mmol/L for 30 min before infection with Mp. The RAW264.7cells were infected with Mp (1∶ 10 MOI) for 4, 8, 16 and 24 h in model group and NAC group, respectively. The intracellular ROS level was analyzed by flow cytometry. The mRNA expressions of NLRP3, ASC and caspase-1 were detected by real-time PCR. The protein levels of NLRP3, ASC and caspase-1 p20 were determined by Western blot. The levels of pro-inflammatory cytokine IL-1β in the supernatant were measured by ELISA. RESULTS: Compared with normal group, the production of ROS were significantly increased at 4, 8, 16 and 24 h after infection, the mRNA expression of NLRP3, ASC and caspase-1 were increased at 8, 16 and 24 h after infection, the protein levels of NLRP3, ASC and caspase-1 p20 were increased at 16 and 24 h after infection, and the releases of IL-1β were increased at 24 h after infection in model group (P<0.01). Compared with the model group, the level of ROS in NAC group decreased, so as the expression of NLRP3, ASC and caspase-1 at mRNA and protein levels and the releases of IL-1β in the supernatant at the corresponding time points. CONCLUSION: Mp may stimulate the ROS production to activate NLRP3 inflammasome in RAW264.7 cells.     

基于模式搜索的轮胎纵向刚度估计方法

提出了一种含有两个参数的轮胎纵向力模型,并通过该模型利用基于模式搜索的最小二乘法对轮胎的纵向刚度和滚动半径进行了动态估计。仿真证明了该算法的可行性。将该算法应用于车辆的实际试验数据,结果证明轮胎的压力与轮胎纵向刚度近似成反比。     

基于组件技术的开放式数控系统体系结构

为实现开放式数控系统的可移植性、可扩展性及可互换性等特性,引进软件工程学思想,提出了基于组件技术的开放式数控系统体系结构。阐述了组件技术的原理及实现技术。软件设计采用双层面设计方式,利用CORBA组件模型并使用星式通讯机制,建立各组件之间的组成及通讯方式。以自主研发的混联式虚拟轴研抛机床为平台,进行了三维曲线加工轨迹仿真实验,表明基于该体系结构的数控系统具有良好的开放性及动态性能。     

谐波小波包自适应分解在故障诊断中的应用

将谐波小波包与匹配追踪方法相结合,提出了谐波小波包自适应分解的新方法。根据信号特征自适应选择谐波小波包字典内的时频原子,可以将非平稳振动信号既不交叠又无遗漏地分解到相互独立的频带上去,算法实现简单,频率分辨率好。通过仿真算例将该法与小波包变换、小波包追踪结果相比较,验证了该方法时频定位性好的优越性。将该方法应用于轴承和转子的故障诊断,结果表明,故障特征提取是有效的。     

苹果水心病的光学无损检测

通过密度法和自行研制的水心病检测仪器对苹果样品进行检测 ,结果表明 :密度无法分离该样品水心病 ;而 1～ 4级水心病的透光强度之间存在显著差异 ,可以进行水心病判断。把近红外 810 nm的计算透光强度2 5 0 0 0作为界限值可以将好果 (1级 )与 3、4级果完全分离 ,2级果有 2 9%分离     

河北省水产品加工业情况调研

水产业是朝阳产业,水产品在国际贸易中市场广阔.全国大农业出口值中水产品占30%,连续6年居大宗农产品首位,国际竞争能力日益凸现.河北省水产业以仅占大农业总产值4%的份额,出口值占到大农业的11.7%,充分显示出水产业的优势.随着改革开放的不断深入发展,国际市场逐步扩大,国际贸易日益兴旺,我国水产品价格相对低廉,在国际市场具有较高竞争力.     

Novel royal jelly proteins identified by gel-based and gel-free proteomics

Royal jelly (RJ) plays an important role in caste determination of the honeybee; the genetically same female egg develops into either a queen or worker bee depending on the time and amount of RJ fed to the larvae. RJ also has numerous health-promoting properties for humans. Gel-based and gel-free proteomics approaches and high-performance liquid chromatography-chip quadruple time-of-flight tandem mass spectrometry were applied to comprehensively investigate the protein components of RJ. Overall, 37 and 22 nonredundant proteins were identified by one-dimensional gel electrophoresis and gel-free analysis, respectively, and 19 new proteins were found by these two proteomics approaches. Major royal jelly proteins (MRJPs) were identified as the principal protein components of RJ, and proteins related to carbohydrate metabolism such as glucose oxidase, α-glucosidase precursor, and glucose dehydrogenase were also successfully identified. Importantly, the 19 newly identified proteins were mainly classified into three functional categories: oxidation-reduction (ergic53 CG6822-PA isoform A isoform 1, Sec61 CG9539-PA, and ADP/ATP translocase), protein binding (regucalcin and translationally controlled tumor protein CG4800-PA isoform 1), and lipid transport (apolipophorin-III-like protein). These new findings not only significantly increase the RJ proteome coverage but also help to provide new knowledge of RJ for honeybee biology and potential use for human health promotion.