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21.
Haitham Sayed Hamed Kayyal Luke Ramsey Salvatore Ceccarelli Michael Baum 《Euphytica》2002,125(2):265-272
A total of 147 simple sequence repeat (SSR) markers (including86 barley and 61 wheat microsatellite markers) were tested for
their segregation in a doubled haploid (DH) and an F2 population of barley. The DH population consisted of 71 doubled haploid lines, developed from F1 plants of a cross between Tadmor and WI2291using isolated microspore culture technique. A genetic linkage map consisting
of 43 microsatellite markers was constructed using the DH population. Particularly on chromosome 4H microsatellite markers
showed distorted segregation ratios. Segregation of DH lines based on molecular markers were compared with segregation of
92 F2 lines from the same cross. The proportion of loci deviating from the expected monogenic segregation ratios in the DH population
was significantly higher (19/43loci, 44%) than in the F2 population (7/43 loci, 16%). The deviation was biased towards the WI2291 parent alleles. In line with this observation, WI2291
was found to perform better than Tadmor in regenerating green plantlets with the isolated microspore-culture technique.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
22.
The homoeologous groups of chromosomes carrying the genes for some morphological traits in wild wheat Triticum turgidum L. ssp. dicoccoides (T. dicoccoides) were determined, but not the actual chromosomes carrying them. The objectives of this study were to investigate the modes
of inheritance, and determine the chromosomes carrying some morphological traits in wild emmer (2n = 28; AABB), the progenitor of most cultivated wheats. To investigate the inheritance of morphological traits, crosses were
made between T. turgidum L. ssp. durum (T. durum cultivar Chartokhmi (IR10) and T. dicoccoides accessions TA1150 and TA1131. F2 seeds from each cross were grown in the field and six qualitative characters were investigated. Purple coleoptile, purple
auricle, purple culm, hairy auricle, hairy rachilla, and fragility of spike were controlled by single dominant genes. To determine
the chromosomal locations, accession TA1131 was crossed with the complete set of LDN D-genome disomic substitution lines.
Assessments of F2 populations showed that chromosomes 7A, 6A, 7B, 5B, 5A and 3B carried genes for purple coleoptile, purple auricle, purple
culm, hairy auricle, hairy rachilla and brittle rachis, respectively. 相似文献
23.
Amr E. El Hakim Yasser E. Shahein Sobhy Abdel-Shafy Amira M. K. Abouelella Ragaa R. Hamed 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(3):243-249
In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval Egyptian ticks, Hyalomma (H.) dromedarii, using a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines against camel tick infestation in rabbits. The rabbits received three intramuscular inoculations of GLPs (20 µg/animal) on days 0, 14, and 28. In the immunoblot analysis, Sera from the immunized rabbits recognized the native GLPs and other proteins from larval and adult H. dromedarii ticks along with those from other tick species such as Rhipicephalus sanguineus but not Ornithodoros moubata. The effects of immunity induced by these GLPs were determined by exposing rabbits to adult H. dromedarii ticks. These results demonstrated that GLP immunization led to a slightly decreased reproductive index and significantly reduced rates of egg hatchability. These results demonstrated that immunization with the purified GLPs can provide protection against infestation by H. dromedarii and some other tick species. Further studies are needed to confirm the effectiveness of immunization with GLPs against other tick species. 相似文献
24.
Existence of diazinon, an organophosphorous pesticide, in river waters of Iran near rice paddy fields has been reported by some authors. The present research aimed to determine the acute toxicity and evaluate the effect of sub-lethal concentrations of diazinon on some biochemical parameters of rainbow trout, Oncorhynchus mykiss after 7, 14 and 28 days. No significant differences were observed in the plasma levels of creatinine among the treatment groups at different sampling intervals. Acetylcholinesterase activity and the levels of total protein, albumin as well as globulin in plasma were significantly reduced at both concentrations tested (p < 0.05). Lactate dehydrogenase activity was only decreased on 7th day in 0.1 mg/L diazinon treatment (p < 0.05). Creatine kinase activity was significantly lower in 0.1 mg/L diazinon group at 14th and 28th sampling periods, whereas its activity significantly increased in fishes exposed to 0.2 mf/L diazinon only on 7th day (p < 0.05). Aspartate aminotransferase, alanine aminotransferase activities and glucose levels in diazinon treated groups were significantly higher than the controlled group at experimental periods (p < 0.05). In conclusion, long-term exposure to diazinon at sub-lethal concentrations induced biochemical alterations in rainbow trout, and offers a simply tool to evaluate toxicity-derived alterations. 相似文献
25.
M. B. Barry J. L. Pham B. Courtois C. Billot N. Ahmadi 《Genetic Resources and Crop Evolution》2007,54(8):1675-1690
Rice genetic diversity partitioning between farms, varieties and, within-variety diversity, were analysed in two villages
of Maritime Guinea with contrasted agroecological conditions. One thousand and two hundred individual plants belonging to
45 accessions collected in eight farms were genotyped using 10 SSR markers. The molecular variance was evenly shared between
and within accessions, while the farm effect was almost nil. Local varieties had a multi-line genetic structure. The number
of multilocus genotypes was proportional to the utilisation rate of the variety in the village. The F
ST values between different accessions of each variety were significant which indicated low genetic consistency in the variety
names. This varietal structure could mainly be explained by the migration phenomenon and the high varietal turnover. Compared
to allelic diversity, multilocus genotypic diversity seemed to be the most suitable indicator of the quantitative distribution
of diversity at different management scales (accession, farm and village). The within- and between-farm F
ST values were in the same order of magnitude. The within-farm diversity was not farm-specific but quantitatively high, i.e.
up to 50% of the total genotypic diversity of a given village. Given the relative importance of the within-variety diversity,
the in situ approach stands out as the most effective solution. As farms do not host specific diversity the in situ approach
could be implemented by working with a small number of farms. 相似文献
26.
Journal of Plant Diseases and Protection - The present study aimed to evaluate the effects of Zataria multiflora essential oil at different concentrations (0, 100, 200, 300 and 500 ppm) on the... 相似文献
27.
Soil salinity is becoming an increasingly serious constraint to plant growth in many parts of the world;this is particularly common in semi-arid and arid zones. This study was conducted to evaluate the... 相似文献
28.
Ahmadi Ebrahim Nazari Hassan Hossini-Fahraji Hamid 《Tropical animal health and production》2019,51(6):1611-1618
Tropical Animal Health and Production - Heat stress can potentially affect most aspects of reproduction in mammals. To our knowledge, no studies have ever been conducted for evaluating the... 相似文献
29.
Rafael De Oliveira Silva Bouda Vosough Ahmadi Sipke Joost Hiemstra Dominic Moran 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2019,136(1):63-73
Ex situ collections offer the potential to reduce extinction risks, affording option to society in maintaining future breeding opportunities for productivity and heritage traits. However, how much should we be seeking to collect and conserve in gene banks, and where? We developed a mathematical model to optimize logistical decisions of breed conservation choices and to evaluate alternative scenarios for efficiently re‐allocating genetic materials currently stored in different European gene banks, allowing for cross‐country collections, cost and cryogenic capacity differentials. We show how alternative allocations for the breeds that are currently stored in 11 European gene banks could reduce overall conservation costs by around 20% by selecting cryogenic banks that have relatively lower combination of fixed and collection costs, and are geographically closer to collection regions. Our results show that centralizing collections in one gene bank would double the costs, relative to collective European collections approaches. We also calculate marginal costs of collections and show that increasing diversity within the gene banks implies in higher costs per conserved breed. 相似文献
30.
M. Kazemi S. Kalateh Jari A. R. Ladan Moghadam 《The Journal of Horticultural Science and Biotechnology》2018,93(1):81-90
With the aim of extending the vase life of cut rose flowers, we investigated whether the application of salicylic acid (SA), pre- and postharvest, could improve this. For this purpose, two individual trials were conducted, using a completely randomised design, with three replications. In the first trial, aqueous solutions of 0, 0.5, 1.0 and 1.5 mM SA were sprayed on roses grown in a controlled greenhouse once the flower buds had appeared, and the second stage was performed two weeks before harvest. The preharvest SA application at 1.5 mM concentration prolonged the vase life of cut roses by improving membrane stability, and decreasing the lipid peroxidation properties. In the second trial, continuous exposure to (0, 0.5, 1.0 and 1.5 mM), and pulses of (0, 5, 10 and 15 mM), SA significantly extended the vase life. Flowers treated with 1.5 (continuous exposure) and 15 mM (pulse) SA showed a significant decrease in weight loss, and antioxidant activity increased, compared to the control solution, but the flowers in the control solution indicated an increase in weight loss. The results show that pre- and postharvest SA application prolonged the vase life of cut roses through improving CAT and POD activity, and decreasing lipid peroxidation. 相似文献