日粮锰水平对辽宁绒山羊与蛋白质代谢相关的血液指标的影响

为了研究日粮中添加锰对辽宁绒山羊蛋白质代谢的影响,试验采用单因子完全随机试验设计,将24只辽宁绒山羊公羊随机平均分为Ⅰ、Ⅱ、Ⅲ组,分别饲喂锰含量为20,40,60 mg/kg的日粮,测定了血液中谷丙转氨酶、谷草转氨酶、总蛋白、尿素氮含量。结果表明:随着锰含量的增加,谷丙转氨酶活性和总蛋白含量显著提高(P<0.05),尿素氮含量显著降低(P<0.05),但谷草转氨酶活性未受显著影响(P>0.05)。综合分析认为,含锰60 mg/kg的日粮对促进辽宁绒山羊的蛋白质代谢较为适宜。     

营养液条件下油酸对黄瓜幼苗生长的影响

水培条件下，研究不同油酸水平对黄瓜幼苗生长的直接影响。与对照相比，生物量和生理指标等表明，油酸10μL/L水平对黄瓜生长几乎没有负面影响，而油酸水平为100，500，1000μL/L则表现出一定的抑制效应。其中，1000μL/L水平中黄瓜的生长明显受到抑制。试验还表明，油酸对黄瓜幼苗没有表现出直接的生长刺激效应。     

抗寒锻炼期间三种楸树叶片激素含量变化

在抗寒锻炼期间,以梓楸、滇楸、灰楸3种楸树为材料,利用酶联免疫吸附方法(ELISA)测定其叶片内源激素IAA(吲哚乙酸)、ABA(脱落酸)、GA3(赤霉素)、ZR(玉米核苷)的含量。结果表明:抗寒锻炼期间,3种楸树叶片ABA含量基本上均呈上升趋势,其它3种激素含量变化趋势不一致。说明在抗寒锻炼期间,3种楸树的抗寒能力逐渐增加。     

Historical processes and landscape context influence genetic structure in peripheral populations of the collared lizard (<Emphasis Type="Italic">Crotaphytus collaris</Emphasis>)

Populations at the periphery of a species’ range often show reduced genetic variability within populations and increased genetic divergence among populations compared to those at the core, but the mechanisms that give rise to this core-periphery pattern in genetic structure can be multifaceted. Peripheral population characteristics may be a product of historical processes, such as founder effects or population expansion, or due to the contemporary influence of landscape context on gene flow. We sampled collared lizards (Crotaphytus collaris) at four locations within the northern Flint Hills of Kansas, which is at the northern periphery of their range, to determine the genetic variability and extent of genetic divergence among populations for ten microsatellite loci (n = 229). We found low genetic variability (average allelic richness = 3.37 ± 0.23 SE; average heterozygosity = 0.54 ± 0.05 SE) and moderate population divergence (average F_ST = 0.08 ± 0.01 SE) among our sample sites relative to estimates reported in the literature at the core of the species’ range in Texas. We also identified differences in dispersal rates among sampling locations. Gene flow within the Flint Hills was thus greater than for other peripheral populations of collared lizards, such as the Missouri glade system where most of the mesic grasslands have been converted to forest since the last glacial retreat, which appears to have greatly impeded gene flow among populations. Our findings signify the importance of considering landscape context when evaluating core-peripheral trends in genetic diversity and population structure.     

Prevalence of antimicrobial residues in raw table eggs from farms and retail outlets in Enugu State,Nigeria

The use of antimicrobial agents in poultry production results in their accumulation in the body tissues and products such as milk and egg. The subsequent accumulation of these drugs and their metabolites in body cells is known as drug residue. This study was designed to determine the prevalence of antimicrobial residues in eggs from poultry farms and retail outlets in Enugu State, Nigeria. Eggs from 25 selected commercial farms and ten retail outlets were screened for the prevalence of antimicrobial residue. Also, structured questionnaires were administered to 25 commercial farms in the state to determine the management practices and the most widely used antimicrobial drugs in farms and possible association between the management practices and the occurrence of antimicrobial residues in eggs from these farms. All the 25 farms surveyed use oxytetracycline. Eggs from nine of the surveyed farms tested positive for antimicrobial residue and three of the ten surveyed farms also tested positive for antimicrobial residue. No association was observed (p 0.05; Fisher’s exact test) between the occurrence of antibiotic residues in eggs and farm size, feed source and housing systems. This study was able to demonstrate the presence of antimicrobial residues in eggs destined for human consumption. Drugs like nitrofurans which has been banned for use in food animals are still very much in use in Enugu State, Nigeria. Antibiotics given as feed additives may give rise to drug residues in food animals.     

供港活猪4种疫病抗体水平监测及免疫效果分析

为掌握广东惠州地区供港活猪主要疫病的免疫状况,我们对供港活猪主要疫病抗体水平作了一次跟踪监测,并对免疫效果进行了分析.2012-2014年,分5次共采取了900头供港猪血清,采用ELISA方法对猪瘟、猪繁殖与呼吸障碍综合征、伪狂犬病、0型口蹄疫的抗体水平进行检测.结果显示,猪瘟抗体免疫合格率在68.9％ ～78.3％之间,口蹄疫在67.2％～82.8％之间,免疫效果不理想;猪繁殖与呼吸障碍综合征抗体免疫合格率在73.9％～ 92.8％之间,伪狂犬病在86.7％ ～ 93.9％之间,免疫效果较理想.     

细胞色素C氧化酶的纯化及动力学性质研究

采用硫酸铵分级沉淀和CytochromeC -Sepharose 4B亲和层析 ,从牛心线粒体中纯化了细胞色素C氧化酶 (CytochromeCOxidase ,简称CCO ) ,其比活性为 1 7 88s- 1 ·mg- 1 ,聚丙烯酰胺凝胶电泳为一条带 ,血红素A (HemeaA)含量达到 1 5 4nmol/mg。纯化后的CCO具有典型的CCO特征 ,还原态CCO峰值出现在 60 5nm和 443nm ,氧化态CCO峰值出现在 598nm和 42 4nm。CCO的稳态动力学参数Vm1、Km1、Vm2 、Km2 分别为 1 42s- 1 、 0 53μmol/L、 2 71s- 1 、 1 81 μmol/L。     

Effect of indomethacin on tumor invasion in human laryngeal cancer Hep-2 cells

AIM: To assess the effect of indomethacin on tumor invasion in a human laryngeal cancer Hep-2 cell line in vitro. METHODS: Hep-2 cells were exposed to indomethacin at different concentrations for 48 h. Then cell growth rate, the colony formation in soft agar medium and cell mobility were examined, and monolayer invasion assay was performed to assess cell invasion index. RESULTS: Preteatment with indomethacin inhibited the colony formation of Hep-2 cells and the cell mobility, and decreased the invasion index. CONCLUSION: Indomethacin can inhibit the invasion of Hep-2 cells.     

青海省泽库县草地现状与畜牧业可持续发展对策

在综述泽库县草地退化现状的基础上,综合分析了草地退化的原因,提出了畜牧业可持续发展的策略.泽库县的草地退化是自然因素和人为因素综合作用的结果.人类活动和气候变化是导致泽库县草地退化、区域生态环境恶化的两大因素,鼠虫害对它们产生的影响起了促进作用.只有合理利用和保护天然草地,优化产业结构,使草地生态系统步入良性循环,才能使泽库县的草地畜牧业协调发展.