Influence of Selenium on Cadmium Toxicity in Cucumber (Cucumis sativus cv. 4200) at an Early Growth Stage in a Hydroponic System

An experiment was established to assess the ability of selenium (Se) to reduce cadmium (Cd) toxicity when tomato was grown hydroponically. A factorial experiment was arranged in a completely randomized design with six replicates in cucumber (Cucumis sativus cv. 4200). The Se was applied at four levels [0 mg L^–1 (Se0), 2 mg L^–1 Se (Se1), 4 mg L^–1 Se (Se2), and 6 mg L^–1 Se (Se3)], whereas Cd was applied at three levels [0 µM Cd (Cd0), 5 µM Cd (Cd1), and 7 µM Cd (Cd2)]. The Se improved the dry weight of roots even when plants were exposed to Cd. Treatment Se1 improved the dry weight of shoots in Cd1 and Cd2. Treatments Se1 and Se2 improved photosynthesis in Cd1. Treatment Se1 significantly improved stomatal conductance in Cd2 at all levels of Se relative to Cd2. The greatest Cd concentration in leaves was observed in Cd2 × Se0 and while Se concentration in solution increased in response to Se1, Se2, and Se3. The greatest Se level reduced Cd uptake the most. Growth and photosynthetic attributes can be negatively affected by Cd, but Se has the ability to buffer, or improve, several attributes.     

Application of biosolids to soil affects Cu and Zn accumulation and antioxidant activity of lettuce (Lactuca sativa L.)

The application of biosolids to agricultural fields is becoming increasingly common. The effect of biosolids on the behavior of metals in different plants has been reported to be variable and ambiguous. A greenhouse experiment was conducted on a soil spiked with four rates of copper (Cu) (0, 50, 100, and 150 mg kg^?1) and zinc (Zn) (0, 150, 300, and 450 mg kg^?1) in lettuce culture. When Cu and Zn were spiked to anaerobically-digested biosolids, their availability in lettuce increased. Root and shoot fresh weight decreased due to a decrease in photosynthetic rate. superoxide dismutase (SOD) and peroxidases (POD) activity increased after the application of Cu and Zn. The bioavailability of Zn in lettuce was greater than that of Cu because of a higher transfer factor in plants and due to a higher endogenous Zn concentration.These results will allow for better fertilization management when biosolids are applied to tomato culture.     

Least limiting water range for different soil management practices in dryland farming in Iran

Integrated evaluation of soil physical properties using the least limiting water range (LLWR) approach may allow a better knowledge of soil water availability. We determined the LLWR for four tillage practices consisted of conventional tillage (CT), reduced tillage (RT), no-tillage (NT) and fallow no-tillage (NT_f). In addition, LLWR was determined for abandoned soils (i.e. control), compacted soils, ploughed compacted soils and abandoned soils with super absorbent polymers (SAPs) application. Soil water retention, penetration resistance (PR), air-filled porosity and bulk density were determined for the 0–5 and 0–25-cm depths. Mean LLWR (0.07–0.08 cm³ cm^?3) was lower in compacted soils than the soils under CT, NT, NT_f, RT, tilled, abandoned and SAP practices but it was not different among tillage practices. The values of LLWR were 0.12 cm³ cm^?3 for NT and CT. LLWR for tilled plots (0.12 cm³ cm^?3) became greater than compacted soils by 1.3 times. Analysis of the lower and upper limits of the LLWR further indicated that PR was the only limiting factor for soil water content, but aeration was not a limiting factor. The LLWR was more dependent on soil water content at permanent wilting point and at PR.     

Oocyte viability and cortical activation under different salt solutions in Prochilodus lineatus (Teleostei: Prochilodontidae)

This study aimed to evaluate the effect of five salt solutions in the maintenance of morphological features of cortical alveolus, hydration and fertilization capacity of Prochilodus lineatus oocytes. For this purpose, five saline solutions were tested: Ringer's solution, Ringer's lactate solution, Hank's balanced salt solution (HBSS), Hank's balanced salt solution without calcium (HBSS without calcium) and solution for salmonid eggs. Oocytes were maintained for 2 hr in saline solution with controlled temperature subsequently evaluated for hydration, cortical activation and fertilization ability. In the evaluation of the fertilization ability, two controls were used: C1—fertilized oocytes after extrusion—and C2—oocytes kept in ovarian fluid and fertilized after 2 hr. There was a significant reduction in the viability of oocytes C2 (28.8% ± 12.9%) compared to C1 (65.3% ± 26.7%), and no significant differences were found between treatments HBSS and HBSS without calcium and C2. Only HBSS and HBSS without calcium maintained the non‐activated state of the gametes, with a fertilization rate of 16.4% ± 6.7% and 5.6% ± 2.3%, respectively; however, they did not extend the viability of oocytes, such that they continued to undergo degradation during the storage period, similar to oocytes retained only in ovarian fluid.     

Comparison of intradermal testing and serum testing for allergen-specific IgE using monoclonal IgE antibodies in 84 atopic dogs

OBJECTIVE: To compare an ELISA measuring serum allergen-specific IgE with intradermal skin testing in canine atopic dermatitis. PROCEDURE: Eighty-four dogs with the clinical diagnosis of atopic dermatitis underwent intradermal skin testing and serum testing for allergen-specific IgE. Tests were performed in a blinded fashion. Positive reactions were compared and the sensitivity and specificity of the serum test (using intradermal skin test as the standard) were determined overall and for individual allergen groups (grass pollens, weed pollens, tree pollens, house dust mites and fleas). RESULTS: The sensitivity of the ELISA overall was 90.4%. Evaluating the individual allergen groups, the sensitivity for dust mite hypersensitivity was 95.1%, for fleas 85.4%, for tree pollens 84.3%, for grass pollens 95.1% and for weed pollens 96.4%. The specificity was 91.6% overall, for dust mites 96.3%, for fleas 92.7%, for tree pollens 95.2%, for grass pollens 94% and for weed pollens 80.7%. CONCLUSION: The evaluated ELISA seemed reliable for the diagnosis of atopy in practice and can be recommended as a screening test prior to intradermal skin testing or for use in dogs when immunotherapy is not a therapeutic option.     

Land suitability evaluation using image processing based on determination of soil texture–structure and soil features

Land evaluation is the process of land performance predictions over time based on land uses and soil features. Traditional methods in determining soil features are proved to be time-consuming and costly. Therefore, in order to overcome these limitations, a simpler automated method using the image segmentation was developed in this study. The method was designed by integrating dynamic region merging and genetic algorithm. An area index was calculated for each soil profile using the automated method. It was used to present the amount of soil coarse particles and thereupon to determine the rating value of text-structure. Using the method, the mean intersection over union of above 0.7 was obtained for detecting the coarse particles which confirms its suitability. Data analysis showed that (a) compared to the Storie-land index (R² = 0.71), the Square root-land index was more correlated to the harvest index (R² = 0.73), and (b) comparing to manual methods, not only the automated text-structure had a higher correlation with the harvest index (R² = 0.64) but also it decreased the determination time (>3.75 times). Furthermore, among the models developed by response surface methodology for estimation of soil features, the developed model for estimation of soil lime showed the highest accuracy (R² = 0.89). In conclusion, since the developed method is more accurate, more economic and faster than the usual manual methods, it can be widely used in land suitability evaluation.