Local population structure of Hymenoscyphus fraxineus surveyed by an enlarged set of microsatellite markers

The population structure of Hymenoscyphus fraxineus, the causal agent of ash dieback, was assessed at four closely located sites in the Czech Republic. To analyse the genetic variation, one Swiss and one Norwegian population with known population structures were selected as reference points. The analysis was performed using 16 previously published and five newly developed microsatellite markers. The quality of the new markers was assessed by sequencing the flanking region, identifying the type of the mutation and analysing the inheritance and linkage between all pairs of loci. In addition, markers were tested on 45 Japanese strains to confirm their usability on native H. fraxineus populations in Asia. One of the new markers was monomorphic in all European populations and one marker exhibited a high percentage of null alleles in the Japanese samples. Twenty markers in the four Czech populations showed lower average gene diversity than in the other two European populations. One Czech population significantly differed from all the others, with a pairwise G_ST of approximately 0.2, the lowest allelic richness and very low average gene diversity, likely resulting from a founder effect. The amount of genetic differentiation between the four neighbouring Czech populations exceeds the expected value from previous Europe‐wide population studies and suggests that local population dynamics can affect the population structure of H. fraxineus.     

银川阅海公园水生植物园规划

银川阅海湿地公园是国家级湿地公园,对银川阅海公园水生植物园的规划建设,可以完善阅海湿地公园的整体布局、功能及生态系统,促进银川阅海公园生态与经济的和谐发展。同时本项目的规划建设对提高银川地区水生植物资源的开发利用、保护生物多样性、扩大银川阅海公园的知名度将产生积极而深远的影响。     

不同氮肥用量对网纹甜瓜产量和品质的影响

针对设施栽培粗网类型网纹甜瓜施氮不合理的问题,试验设置3个施氮水平：0、100、200 kg/hm²,采用田间调查与室内检测相结合的方法,测定了植株的株高、茎粗、叶片纵横径和坐果节位比较植株长势;测定伸蔓期、结果期、网纹形成期和果实成熟期的顶3叶叶片纵横径、叶色、SPAD值和叶片氮含量;以及甜瓜产量和品质等指标。结果表明：粗网网纹甜瓜的施氮量为100 kg/hm²时品质最佳,产量虽略低于200 kg/hm²的处理,但商品果率最高;顶3叶的叶色、叶片纵横径和SPAD值随着氮肥的施用量增加均表现出相关性,初步摸索了顶3叶对氮素的响应规律,指导田间生产。     

Effects of Continuously Applied Vermicompost on Media Properties,Growth, Yield,and Fruit Quality of Two Tomato Varieties

In the first and second seasons, growth and yield of tomato were also influenced by different vermicompost (VC) rates and reused VC. Obviously, increasing VC proportion led to the augmentation of electrical conductivity (EC), nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) in planting media in both seasons. However, in the first season, the nutrient absorbent ability of tomato was remarkably higher compared to those in the second season due to higher available nutrient in the media of the first season. This may influence the shoot dry weight as well as the yield of tomato in both seasons. In T4 treatment, the yield attained the highest value in the first season for both varieties with the values of 562.3 and 812.4 g/plant for small honey (V1) and graces (V2), respectively. In the second season, the N, P, K, Ca, and Mg in media significantly decreased compared to those in the first season (496.4 g/plant-V1 and 508.3 g/plant-V2). In comparison, the yields in the first season were higher those in second season by 13.2% and 59.8% for V1 and V2, respectively. However, in the second season, the maximum yield found in T5 (508.3 g/plant-V1 and 682.2 g/plant-V2) and declining by 10.6 and 19.1% compared to those in the first season for V1 and V2, respectively. This can be explained that amounts of nutrients from the media in the first season had supported the growth and development of tomato, resulting deficiency of nutrients in the media for the second season.     

基于SI-Albedo特征空间的干旱区盐渍化土壤信息提取研究——以克里雅河流域绿洲为例

选取塔里木南缘克里雅河流域绿洲为研究靶区,利用Landsat-ETM+卫星图像数据和野外调查数据分析盐渍化土壤与地表反照率(Albedo)、土壤盐分指数(SI)之间的关系。回归分析发现,盐渍化土壤在SI-Albedo特征空间分布具有显著规律,即非盐渍化土壤呈团状分布;轻、中度盐渍化土壤具有线性分布特征;非盐渍化土壤与轻度盐渍化土壤分异明显。结合分异规律,编制分类算法模型,得到研究区盐渍化土壤信息提取结果,并与传统监督最大似然分类法结果进行对比分析。结果表明,在SI-Albedo特征空间中定量快速提取盐渍化土壤信息的总体效果较好,对准确且自动提取干旱区盐渍化土壤信息以及区域尺度盐渍化遥感监测研究具有重要意义。     

Effect of Vermicompost in Media on Growth,Yield and Fruit Quality of Cherry Tomato (Lycopersicon esculentun Mill.) Under Net House Conditions

Effects of VC application on tomato production and media properties can last for the third successive season, which was verified in this study to evaluate effects of VC application on growth, productivity and fruit quality of cherry tomato for the third season. Six treatments were designed similarly in the first and second seasons (unchanged design compared to the previous season), including: VC with rice husk ash (RHS) and coconut fiber (CF) 0% VC+ 50% RHS +50% CF (T1- control), respectively. 20%VC + 40% RHS + 40% CF(T2), 40% VC+ 30% RHS + 30% CF(T3), 60% VC + 20% RHS + 20% CF(T4), 80% VC + 10% RHS + 10% CF(T5), and 100% VC(T6). The results showed that the pH, EC, N, P, K, Ca, and Mg available in media were increased with the addition of VC. This finding revealed that the addition of VC significantly improved the physic-chemical media properties, increasing the EC and also increasing the macronutrients in the media resulting in substantial increased the yield and quality of tomato fruits. It is concluded that EC of VC was the key factor for the positive increase of the productivity and quality of tomato and the salinity (EC at 3.52 dS/m) causing restriction of water to fruits that resulted in the increase of the total soluble solids in tomato fruit and without affecting yield reduction.     

Policosanol Content and Composition of Korean Rice (Oryza sativa L.) Cultivars

Policosanols (PCs) are a group of long‐chain alcohols that have been reported to have many beneficial physiological activities. In this study, the total content and composition of PCs in 15 rice (Oryza sativa L.) cultivars from Korea were characterized by gas chromatography–time‐of‐flight mass spectrometry. This method proved to be sufficiently precise and accurate with respect to the degree of endogenous biological variability found in the rice samples. Octacosanol (C28) and triacontanol (C30) were the major components of PCs in all cultivars. In addition, there were positive correlations among the determined PC contents. Given its high PC content, the Heughyangbyeo cultivar may appear to be a good candidate for future breeding programs.     

Metabolic blood profile of beef heifers during oestrous and non‐oestrous states

Haematological metabolic profiles in heifers could contribute to the development of proxies for oestrous detection and provide clues to further characterize biological changes during oestrus. One hundred and seven beef heifers were observed for oestrous behaviour twice daily for 124 days. Feed intake and productive performance (body weight and composition) traits were measured, and feed efficiency was determined using residual feed intake (kg DM/day). Blood plasma samples were collected when signs of oestrus were observed and every 30 ± 2 days. Heifers were considered in oestrus (n = 71) when plasma progesterone concentrations were <0.6 ng/ml. Least square means of blood metabolic parameters were compared between oestrous and non‐oestrous states and within oestrous groups according to performance traits and age. Heifers in oestrus exhibited higher concentrations of alkaline phosphatase, aspartate aminotransferase (AST), beta‐hydroxybutyric acid, creatine kinase (CK) and triiodothyronine (T3) than heifers in non‐oestrus. Heifers in oestrus revealed lower osmolality and concentrations of calcium, sodium and total protein than during non‐oestrus. Younger (and smaller) heifers had greater concentrations of CK, gamma‐glutamyl transferase (GGT), glucose and sodium than the older heifers. Heifers with lower fatness had increased osmolality and concentrations of cholesterol, CK, phosphorus, sodium and reduced T3 levels. Feed efficient heifers had greater levels of AST, cholesterol and GGT than inefficient heifers. Blood plasma parameters may be complementary to oestrous detection upon further validation; effects of age, feed efficiency, body size and body composition should be considered to optimize this haematological assessment.