基于ISSR分子标记技术的山核桃幼胚DNA甲基化初步研究

用简单序列重复区间扩增(inter-simple sequence repeat,ISSR)DNA分子标记技术对山核桃Carya cathayensis不同发育阶段幼胚(第9周至13周)的DNA进行甲基化状态检测。结果表明:17个引物在各个发育时期山核桃幼胚样品中共扩增检测到的128个甲基化位点。甲基化比例随着幼胚的发育逐渐升高,5个时期幼胚甲基化比例依次为23.44%,25.78%,28.90%,40.62%和42.19%。进一步研究表明:5个时期均存在全甲基化位点和半甲基化位点,其中,全甲基化位点所占比例分别为7.81%,8.59%,16.40%,24.22%和25.00%;半甲基化位点所占分别为15.62%,17.19%,12.50%,16.41%,17.19%。因此,山核桃幼胚5个发育时期在基因组DNA甲基化水平上存在着较大差异(P<0.01)。     

基于EnMAP-Box的遥感图像分类研究

采用2007年6月云南省勐腊县TM遥感数据,利用EnMAP-box进行了支持向量机的图像分类研究,以网格搜索法寻找最优参数,在设定的范围内,求得了最优C和g参数,用此参数进行支持向量机的遥感图像土地覆盖分类。结果表明：SVM方法较最大似然分类方法具有较高的分类精度,特别是阔叶林和橡胶林的精度明显优于最大似然分类方法;对于面积较小的次要类型,2种分类方法的精度基本保持一致;SVM的总体精度相对于最大似然分类提高了11.9％。     

河流与开放空间耦合的城市绿地生态网络构建

随着我国城市化进程的加快,城市生态环境面临着越来越严峻的考验,为了促进城市的可持续发展,构建城市绿地生态网络、维护城市生态系统的稳定性成为众多领域研究的重点。河流作为城市绿地生态网络的核心空间结构,开放空间作为绿地生态网络的基础,二者关系的研究对构建城市绿地生态网络具有重要意义。在研究河流与开放空间耦合关系的基础上,结合对城市绿地生态网络含义、功能机制、评价指标的分析,以南阳市为例,提出河流与开放空间耦合的城市绿地生态网络构建方法。     

中西方传统婚礼服饰文化相互借鉴之研究

随着中西方文化交流的日益加强,中西方传统的婚礼服饰文化在保留本国特色的同时也逐渐在相互借鉴.本文通过对中国的汉族传统婚礼服饰与西方国家婚礼服饰及文化背景进行对比研究,探讨中西方婚礼服饰文化相互借鉴对双方的影响.     

Effect of grape seed proanthocyanidin on cisplatin-induced nephrotoxicity in mice

AIM:To study the protective effect of grape seed proanthocyanidin (GSP) on nephrotoxicity induced by cisplatin (CP) in mice. METHODS:Male C57 mice were randomly divided into 4 groups: control group (N group, n=10), CP group (intraperitoneal injection of CP at dose of 20 mg/kg, n=20), GSP group (intragastric administration of GSP at dose of 500 mg/kg, n=15) and CP+GSP group (intragastric administration of GSP 30 min prior to intraperitoneal injection of CP and intragastric administration of the same dose of GSP 72 h later, n=20). On the 5th day after CP treatment, blood and kidney samples were collected. The renal pathological changes were examined by HE staining. The protein expression of glucose-regulated protein 78 (GRP78) and phosphorylated extracellular signal-regulated kinase (p-ERK) was examined by Western blotting and immunohistochemical staining. RESULTS:Renal index, blood urea nitrogen and serum creatinine were significantly higher in CP group than those in N group. The renal tissues were heavily damaged in CP group. The protein expression of GRP78 and p-ERK increased in CP group compared with N group. GSP treatment alleviated the increase in the renal index, blood urea nitrogen and serum creatinine. The damages of the renal tissues were attenuated. The protein expression of GRP78 and p-ERK was obviously reduced in CP+GSP group compared with CP group. CONCLUSION:GSP prevents kidney from CP-induced damage by suppression of endoplasmic reticulum stress.     

Strontium renelate promotes osteogenesis via regulation of Sonic hedgehog signaling molecules in rat bone marrow mesenchymal stem cells

AIM：To study the role of Sonic Hedgehog (Shh) in strontium ranelate (Sr)-induced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). METHODS：BMSCs was isolated from 4-week-old rats by adherent culture. The cells in the 3rd～5th generations were induced to differentiate into obteoblasts, and then were treated with different concentrations of Sr and cyclopamine (Cy). The activity of alkaline phosphatase (ALP) was mea-sured by colorimetry. Mineralized nodules were observed by alizarin red staining. The cellular Shh and Runx2 expression was detected by Western blotting. RESULTS：Sr at concentration of 3 mmol/L increased the activity of ALP and induced the formation of mineralized nodules. Sr at concentrations ranging from 0.1 to 5 mmol/L increased the expression of Shh and Runx2 in the BMSCs at 7 d. Furthermore, the peak expression of Shh occurred following the exposure of Sr (1 mmol/L) or Runx2 (3 mmol/L). On the other hand, Sr at concentration of 1 mmol/L showed a time-dependent increase in the expression of Shh and Runx2 from 1 d to 7 d. Cy at concentration of 10 μmmol/L not only obviously inhibited Sr-induced expression of Shh and Runx2, but also antagonized the increase in the ALP activity and mineralization induced by Sr in the BMSCs. CONCLUSION：Sr promotes osteogenic differentiation of BMSCs by increasing the expression of Shh and Runx2.     

地理标志产品“马家沟芹菜”品牌的形成与发展

“马家沟芹菜”作为蔬菜类地理标志农产品的典型代表,经过多年的培育,已经成为我国知名的品牌。本文从“马家沟芹菜”的历史形成、品牌培育以及发展和保护等方面进行系统阐述。     

不同喷灌灌水上限对小白菜生长、品质的影响

研究了喷灌和大棚条件下小白菜不同土壤含水量(分别为田间持水量的60%~100%、60%~90%、60%~80%、60%~70%)处理对小白菜生长、品质的影响。结果表明,以田间持水量的90%、100%作为灌水量上限条件下小白菜地上部干鲜质量、叶面积、叶片数均最高。小白菜可溶性糖含量以田间持水量90%和80%上限处理最高,可溶性蛋白含量以田间持水量100%上限处理最高,90%及80%上限处理其次,维生素C含量以田间持水量90%处理最高。小白菜喷灌指标以田间持水量90%作为上限(下限60%)最合适。     

豇豆叶绿体微卫星标记的开发及其在近缘种的通用性研究

从公共数据库中下载豇豆叶绿体基因组,对其进行全序列分析,揭示豇豆cpSSR的分布特点和基序特征,进一步研究豇豆cpSSR在豆类植物中的通用性。研究结果表明,豇豆叶绿体基因组上共有52个微卫星,主要分布于LSC区,且以A/T为单碱基重复基序的微卫星为主;从豇豆的52个叶绿体微卫星中筛选出8对多态性豇豆cpSSR引物,在豌豆、利马豆和菜豆中均成功扩增,表明这些豇豆cpSSR引物在其豆类近缘种中具有较好的通用性。     

清镇市黄瓜新优品种引进比较试验

为筛选适合清镇市栽培的黄瓜品种,采用设施栽培,以津研4号为对照,观察中农28、C66等7个黄瓜品种在清镇市栽培的表现。试验结果表明,8个品种产量排序为中农28C66A6A7C32中农18中农116津研4号,中农28比对照增产11.0%;各个品种单果质量差异不大;中农28和中农116对霜霉病的抗性较强,病情指数分别为16.4和16.5;中农28的抗病性及商品性均较好,适合在清镇市大范围推广。