New screening test to predict the potential impact of ivermectin-contaminated cattle dung on dung beetles

According to European Union recommendations, a test method has been developed to evaluate the effects of veterinary pharmaceuticals on dung feeding insects. This test method was evaluated with the dung beetle Aphodius constans by using fecal residues of ivermectin after a pour-on administration. Dung of different age (and thus containing different concentrations of ivermectin) as well as mixtures of highly-contaminated spiked dung with untreated control dung were studied in five test runs in two laboratories. The concentration of ivermectin (active substance; a.s.) in the dung samples was verified analytically. The main test endpoint was the survival of first instar larvae. The LC50 using dung directly obtained from treated cattle ranged from 470 to 692 microg a.s. kg(-1) dung (dry weight; d.w.) and 67 to 97 microg a.s. kg(-1) dung (fresh weight; f.w.). Using mixtures, the outcome of two tests was almost identical: 770 to 781 microg a.s. kg(-1) dung (d.w.); 109 to 132 microg a.s. kg(-1) dung (f.w.). In comparison to the LC50 values obtained when ivermectin was spiked in control dung at several concentrations (LC50 880-985 microg a.s. kg(-1) dung (d.w.)), the LC50 values were again very similar. Three conclusions can be drawn from these results. The proposed test method seems to be robust and allows for the initiation of an international validation process (including ringtesting). Because of only small differences found in tests in which the test substance was spiked into control dung and those in which dung from treated cattle was applied, the use of a standard test method is proposed. The effects of ivermectin on ecologically relevant dung beetles obtained in a standardised test method reflect the results from field studies and are in the range of environmentally relevant concentrations.     

Cholera toxin promotes the generation of semi-mature porcine monocyte-derived dendritic cells that are unable to stimulate T cells

Cholera toxin (Ctx) is a powerful mucosal adjuvant with potential applications for oral vaccination of swine. Dendritic cells (DC) play a key role in the decision between immunity and tolerance, and are likely target cells for mediating Ctx functions in vivo. Therefore, we examined the capacity of Ctx to enhance stimulatory activity of porcine monocyte-derived DC (MoDC). Ctx promoted the development of a semi-mature DC phenotype, with decreased levels of MHC class II and CD40, but increased CD80/86 expression. These changes were associated with activation of extracellular signal-regulated kinase (ERK), but not NFkappaB or c-Jun N-terminal kinase (JNK). Functionally, Ctx-priming greatly diminished T cell stimulatory capacity both in antigen-specific and superantigen-induced proliferation assays. The lower proliferation rate was not due to increased apoptosis of either DC or T cells. Ctx suppressed TNFalpha secretion by MoDC, but induced IL-10 production. The observed effects on T cell proliferation could only be partially mimicked by IL-10 alone. However, addition of recombinant TNFalpha to co-cultures of Ctx-primed MoDC and lymphocytes restored lymphocyte proliferation in a concentration-dependent manner. Ctx-primed DC were not actively tolerogenic, since they could not suppress proliferative T cell reactions induced by untreated DC.     

Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.     

拖拉机带辅助油缸的悬挂机构速度分析

运用解析法对带有辅助油缸装置的半分置式拖拉机悬挂机构进行提升速度分析,推导主油缸与辅助油缸的同步速比计算的数学模型,并应用计算机编程优化悬挂机构的结构参数和工作参数。     

基于PDM犁的智能CAD设计系统

讨论基于产品数据管理(PDM)犁的智能CAD设计系统基本框架及其3个组成部分：设计专家系统、产品数据管理和犁的计算机辅助设计。研究了各部分的功能和技术实现，设计专家系统用于犁的参数化设计过程中参数的选择和装配图装配合理性的判断，PDM主要管理犁的辅助设计相关的数据和文件以及专家系统知识。在此基础上，介绍了集成专家系统和产品数据管理犁的计算机辅助设计软件开发。     

棉田膜内与膜间土壤溶液含盐量的变化

用定期取表层土样测定其电导率和在不同深度埋设盐分传感器探头的方法 ,在作物生长季节内 ,监测了棉田膜内和膜间不同深度的土壤溶液含盐量变化。结果为 :1棉田膜内与膜间表层土壤和 1 0 cm、30 cm深土壤溶液含盐量变化较大 ,且规律相同。未灌水时上升 ,灌水后迅速下降。膜间未灌水期间的含盐量上升幅度大于膜内 ,灌水对膜内和膜间表土洗盐、1 0 cm、30 cm深土壤溶液含盐量的降低作用没有差别。降雨可引起膜内和膜间 1 0 cm深土壤溶液电导率的变化 ,这个影响也是膜间大于膜内。 2 60 cm深的土壤溶液不存在明显的盐分累积现象 ,灌水可使这里的土壤溶液含盐量缓慢降低 ,膜间的降低过程快于膜内     

黄土高原半干旱区退耕还林对土壤微量元素的影响

为了探明黄土高原半干旱区退耕还林对土壤微量元素(Cu、Zn、Mn、Mo、Fe)含量的影响及其与影响因子间的关系,以甘肃省关川河流域退耕还林地和农地为研究对象,使用有效性评价、通径分析等方法研究土壤微量元素与土壤环境变量间的关系。结果表明:退耕后土壤微量元素含量随土层深度增加而减少,在表层土壤中呈一定的富集现象。退耕地Cu、Mn、Mo的有效性指数高于Zn、Fe,侧柏林地有效性综合指数高于云杉林地。偏相关分析显示,退耕还林区土壤微量元素有效态含量的主要影响因素为土壤有机质、全钾、全氮与土壤含水率;回归分析显示,土壤环境因子可解释38.1%~73.8%有关土壤微量元素含量的变异;通径分析表明,土壤全钾对土壤微量元素的直接作用最大,土壤全氮对微量元素的间接作用最大;决策分析表明,土壤全钾与土壤有机质是退耕还林区微量元素含量的决策变量,而土壤含水率是土壤微量元素的限制变量。     

猪丹毒病的防制

<正>猪丹毒是猪丹毒杆菌引起的一种急性、热性传染病。其临床症状与剖检特征为高热,急性败血症,亚急性皮肤疹块,慢性疣状心内膜炎及皮肤坏死与多发性非化脓性关节炎。主要侵害架子猪。1病原其病原为猪丹毒杆菌,又称红斑丹毒丝菌,为G+细长的小杆菌,不形成芽孢和荚膜,不能运动。在病料内的细菌常单在、成对或成丛状排列;在慢性病猪的心内膜,多呈长丝状。病原体杆菌寄生在病猪的所有器官。扁桃体、肠道、胆汁、骨髓体以及病猪的唾液、     

兽医诊疗失误的影响因素与应对思考

兽医诊疗是促进养殖业稳步发展的一项重要工作.但是,当前兽医诊疗工作受多种因素的影响,时常发生诊疗失误的情况,极容易延误诊疗最佳时机,导致诊疗失败,给养殖户带来经济损失.本文对兽医诊疗失误的表现形式进行分析,并从主观与客观两个层面探究影响诊疗失误的因素,进而提出应对策略,期望对规避诊疗失误风险,提高兽医诊疗水平有所帮助.     

桂花栽培与管理技术

从单干桂花栽培、受冻桂花的复壮及桂花叶枯病的防治等方面总结了桂花栽培管理技术。